Journal: Virchows Archiv

Article Title: Preanalytical variables and performance of diagnostic RNA-based gene expression analysis in breast cancer

doi: 10.1007/s00428-014-1652-0

Figure Lengend Snippet: EndoPredict test results achieved after different storage times and temperatures before fixation. The solid line represents the reference score which was derived from the mean EP score of the tissue sections that have been stored for 10 min at 20 °C before fixation. Dotted lines represent the reference score ±3× its standard deviation as determined by precision study . Measurements were performed in duplicate ( n = 2). Mean values are presented, with standard errors of the means indicated by bars

Article Snippet: EndoPredict tests (Sividon Diagnostics, Cologne, Germany) were performed as previously described using SuperScript III PLATINUM One-Step Quantitative RT-PCR System (Life Technologies, Darmstadt, Germany) and VERSANT kPCR Molecular System (Siemens Healthcare Diagnostics, Tarrytown, USA) [ , ].

Techniques: Derivative Assay, Standard Deviation

Journal: Virchows Archiv

Article Title: Preanalytical variables and performance of diagnostic RNA-based gene expression analysis in breast cancer

doi: 10.1007/s00428-014-1652-0

Figure Lengend Snippet: EndoPredict test results achieved after different fixation times. The solid line represents the reference score which was derived from the mean EP score of the tissue sections that have been fixed for 20 h in neutral buffered formalin. Dotted lines represent the reference score ±3× its standard deviation as determined by precision study . Measurements were performed in duplicate ( n = 2). Mean values are presented, with standard errors of the means indicated by bars

Article Snippet: EndoPredict tests (Sividon Diagnostics, Cologne, Germany) were performed as previously described using SuperScript III PLATINUM One-Step Quantitative RT-PCR System (Life Technologies, Darmstadt, Germany) and VERSANT kPCR Molecular System (Siemens Healthcare Diagnostics, Tarrytown, USA) [ , ].

Techniques: Derivative Assay, Standard Deviation

Journal: Virchows Archiv

Article Title: Preanalytical variables and performance of diagnostic RNA-based gene expression analysis in breast cancer

doi: 10.1007/s00428-014-1652-0

Figure Lengend Snippet: Correlation of the relative expression values of the EndoPredict genes of interest between whole sections and manually microdissected sections

Article Snippet: EndoPredict tests (Sividon Diagnostics, Cologne, Germany) were performed as previously described using SuperScript III PLATINUM One-Step Quantitative RT-PCR System (Life Technologies, Darmstadt, Germany) and VERSANT kPCR Molecular System (Siemens Healthcare Diagnostics, Tarrytown, USA) [ , ].

Techniques: Expressing

Journal: Virchows Archiv

Article Title: Preanalytical variables and performance of diagnostic RNA-based gene expression analysis in breast cancer

doi: 10.1007/s00428-014-1652-0

Figure Lengend Snippet: Correlation between the EP ( a ) and EPclin ( b ) scores of paired whole and tumor-enriched, manually microdissected tissue sections from ER+/HER2− breast cancer patients ( n = 39). Pearson correlation coefficient of EP scores was 0.97 with a concordance of EndoPredict risk classification of 87 %. Pearson correlation coefficient of EPclin scores was 0.98 with a concordance of EndoPredict risk classification of 95 %. Black filled circles represent tissue samples with TCC ≥30 %, open circles tissue sections with TCC <30 %

Article Snippet: EndoPredict tests (Sividon Diagnostics, Cologne, Germany) were performed as previously described using SuperScript III PLATINUM One-Step Quantitative RT-PCR System (Life Technologies, Darmstadt, Germany) and VERSANT kPCR Molecular System (Siemens Healthcare Diagnostics, Tarrytown, USA) [ , ].

Techniques:

Journal: Virchows Archiv

Article Title: Preanalytical variables and performance of diagnostic RNA-based gene expression analysis in breast cancer

doi: 10.1007/s00428-014-1652-0

Figure Lengend Snippet: Mean difference of relative expression values (delta Cq value [nonstored]-delta Cq value [stored]) of the EndoPredict genes of interest between nonstored and stored FFPE tissue sections

Article Snippet: EndoPredict tests (Sividon Diagnostics, Cologne, Germany) were performed as previously described using SuperScript III PLATINUM One-Step Quantitative RT-PCR System (Life Technologies, Darmstadt, Germany) and VERSANT kPCR Molecular System (Siemens Healthcare Diagnostics, Tarrytown, USA) [ , ].

Techniques: Expressing

Journal: Virchows Archiv

Article Title: Preanalytical variables and performance of diagnostic RNA-based gene expression analysis in breast cancer

doi: 10.1007/s00428-014-1652-0

Figure Lengend Snippet: Correlation of EP scores between stored and nonstored FFPE tissue sections. EP scores have been determined at the beginning of the study, after 12 months at +4 °C ( a ) or after 12 months at +20 °C ( b ), respectively. Pearson correlation coefficients were 0.99 and >0.99 with a concordance of EndoPredict risk classification of 100 %

Article Snippet: EndoPredict tests (Sividon Diagnostics, Cologne, Germany) were performed as previously described using SuperScript III PLATINUM One-Step Quantitative RT-PCR System (Life Technologies, Darmstadt, Germany) and VERSANT kPCR Molecular System (Siemens Healthcare Diagnostics, Tarrytown, USA) [ , ].

Techniques:

Journal: BMC Cancer

Article Title: Decentral gene expression analysis: analytical validation of the Endopredict genomic multianalyte breast cancer prognosis test

doi: 10.1186/1471-2407-12-456

Figure Lengend Snippet: RNA input range and reproducibility of EndoPredict. ( A ) EP scores depending on amount of input RNA. RNAs from 6 different FFPE samples were diluted and EP scores were assessed dependent on RNA input (C q -ARG as surrogate marker). 95% confidence intervals (CI) of EP scores calculated from the noise model are indicated. ( B ) Correlation between C q -ARG and total RNA concentration assessed by RIBOGREEN assay. Lower RNA input limit is indicated by dotted lines. ( C ) Reproducibility of 160 EP scores assessed in three different RNA samples (low risk, close to the decision point, high risk) over time (11 different working days distributed over 21 calendar days). Individual EP measurement results are indicated by dots.

Article Snippet: Here, we analytically validated the EndoPredict multianalyte gene expression assay according to the adapted guideline MM17-A of the Clinical and Laboratory Standards Institute (CLSI) addressing the analytical validation of nucleic acid-based qualitative and semiquantitative multiplex assays [ ].

Techniques: Marker, Concentration Assay