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  • 99
    Thermo Fisher end dna labeling kit
    End Dna Labeling Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1970 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher end labeling kit
    End Labeling Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega deoxynucleotidyl transferase dutp nick end labeling tunel assay
    Autophagy impairs the function of ASPP2 on inducing apoptosis. Huh7.5 cells were treated with autophagy inhibitor 3-MA and transfected with ASPP2-p. (A) Immunofluorescence assay was used to detect the effect of 3-MA on inhibiting ASPP2-induced autophagy. (B) <t>TUNEL</t> and Calcein AM/PI assays were used to detect the effect of autophagy inhibition via 3-MA on ASPP2-induced apoptosis and cell death. The values represent the mean ± standard deviation of three independent experiments. ASPP2, apoptosis-stimulating protein of p53; TUNEL, terminal <t>deoxynucleotidyl</t> transferase <t>dUTP</t> nick-end labeling; AM, acetoxymethyl ester; PI, propidium iodide; 3-MA, 3-methyladenosine; ASPP2-p, ASPP2 plasmid.
    Deoxynucleotidyl Transferase Dutp Nick End Labeling Tunel Assay, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega deadend fluorometric tunel system
    Autophagy impairs the function of ASPP2 on inducing apoptosis. Huh7.5 cells were treated with autophagy inhibitor 3-MA and transfected with ASPP2-p. (A) Immunofluorescence assay was used to detect the effect of 3-MA on inhibiting ASPP2-induced autophagy. (B) <t>TUNEL</t> and Calcein AM/PI assays were used to detect the effect of autophagy inhibition via 3-MA on ASPP2-induced apoptosis and cell death. The values represent the mean ± standard deviation of three independent experiments. ASPP2, apoptosis-stimulating protein of p53; TUNEL, terminal <t>deoxynucleotidyl</t> transferase <t>dUTP</t> nick-end labeling; AM, acetoxymethyl ester; PI, propidium iodide; 3-MA, 3-methyladenosine; ASPP2-p, ASPP2 plasmid.
    Deadend Fluorometric Tunel System, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 6827 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher deoxynucleotidyl transferase dutp nick end labeling tunel assay
    Autophagy impairs the function of ASPP2 on inducing apoptosis. Huh7.5 cells were treated with autophagy inhibitor 3-MA and transfected with ASPP2-p. (A) Immunofluorescence assay was used to detect the effect of 3-MA on inhibiting ASPP2-induced autophagy. (B) <t>TUNEL</t> and Calcein AM/PI assays were used to detect the effect of autophagy inhibition via 3-MA on ASPP2-induced apoptosis and cell death. The values represent the mean ± standard deviation of three independent experiments. ASPP2, apoptosis-stimulating protein of p53; TUNEL, terminal <t>deoxynucleotidyl</t> transferase <t>dUTP</t> nick-end labeling; AM, acetoxymethyl ester; PI, propidium iodide; 3-MA, 3-methyladenosine; ASPP2-p, ASPP2 plasmid.
    Deoxynucleotidyl Transferase Dutp Nick End Labeling Tunel Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Promega deoxynucleotidyl transferase mediated dutp nick end labeling tunel assay
    PAb induced apoptosis in vivo as revealed by <t>TUNEL</t> assay. a – c Sections from the tumour-bearing mice treated with NS, control IgG, or PAb, were stained with <t>FITC-dUTP</t> as described in materials and methods (×200). d An apparent increase in the number of apoptotic cells and apoptotic index was observed within the remaining tumours tissue of mice treated with PAb compared to mice injected subcutaneously with control IgG, *represents significant difference between PAb treatment group mice with the NS group and control IgG group mice ( P
    Deoxynucleotidyl Transferase Mediated Dutp Nick End Labeling Tunel Assay, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Boehringer Mannheim dig oligonucleotide 3 end labeling kit
    PAb induced apoptosis in vivo as revealed by <t>TUNEL</t> assay. a – c Sections from the tumour-bearing mice treated with NS, control IgG, or PAb, were stained with <t>FITC-dUTP</t> as described in materials and methods (×200). d An apparent increase in the number of apoptotic cells and apoptotic index was observed within the remaining tumours tissue of mice treated with PAb compared to mice injected subcutaneously with control IgG, *represents significant difference between PAb treatment group mice with the NS group and control IgG group mice ( P
    Dig Oligonucleotide 3 End Labeling Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher end dna labelling kit
    PML immunofluorescence combined with Tel-FISH in U2OS, CM and QGP cell lines. U2OS cell line represents a universal positive control for ALT mechanism. CM cell line, a <t>TERTp</t> wild-type cell line, presented a high co-localization of telomeric <t>DNA</t> with PML exhibiting an ALT positive phenotype. QGP1, the TERTp mutated cell line does not present frequent co-localization of telomeric DNA with being ALT negative. These findings recapitulate the observations in our series of PETs.
    End Dna Labelling Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Trevigen deoxynucleotidyl transferase dutp nick end labeling tunel assay
    PML immunofluorescence combined with Tel-FISH in U2OS, CM and QGP cell lines. U2OS cell line represents a universal positive control for ALT mechanism. CM cell line, a <t>TERTp</t> wild-type cell line, presented a high co-localization of telomeric <t>DNA</t> with PML exhibiting an ALT positive phenotype. QGP1, the TERTp mutated cell line does not present frequent co-localization of telomeric DNA with being ALT negative. These findings recapitulate the observations in our series of PETs.
    Deoxynucleotidyl Transferase Dutp Nick End Labeling Tunel Assay, supplied by Trevigen, used in various techniques. Bioz Stars score: 92/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Promega terminal deoxynucleotidyl transferase dutp nick end labeling
    PML immunofluorescence combined with Tel-FISH in U2OS, CM and QGP cell lines. U2OS cell line represents a universal positive control for ALT mechanism. CM cell line, a <t>TERTp</t> wild-type cell line, presented a high co-localization of telomeric <t>DNA</t> with PML exhibiting an ALT positive phenotype. QGP1, the TERTp mutated cell line does not present frequent co-localization of telomeric DNA with being ALT negative. These findings recapitulate the observations in our series of PETs.
    Terminal Deoxynucleotidyl Transferase Dutp Nick End Labeling, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Promega deadend fluorometric terminal deoxynucleotidyl transferase dutp nick end labeling tunel system
    Increased apoptotic cell death following overventilation (OV) in lungs lacking TRIM72 and/or Cav1. A : representative terminal <t>deoxynucleotidyl</t> transferase <t>dUTP</t> nick-end labeling <t>(TUNEL)</t> staining on lung tissue at ×100; WT, TRIM72 KO , Cav1 KO , and
    Deadend Fluorometric Terminal Deoxynucleotidyl Transferase Dutp Nick End Labeling Tunel System, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling tunel staining
    Increased apoptotic cell death following overventilation (OV) in lungs lacking TRIM72 and/or Cav1. A : representative terminal <t>deoxynucleotidyl</t> transferase <t>dUTP</t> nick-end labeling <t>(TUNEL)</t> staining on lung tissue at ×100; WT, TRIM72 KO , Cav1 KO , and
    Terminal Deoxynucleotidyl Transferase Mediated Deoxyuridine Triphosphate Nick End Labeling Tunel Staining, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA deoxynucleotidyl transferase dutp nick end labeling tunel assay
    Percentage of sperm with DNA fragmentation in male partners of women with recurrent implantation failure ( a and b ), recurrent miscarriage ( c and d ), and in a group of recent fathers (control) ( e and f ) as measured by sperm chromatin disruption test (left hand graphs) and terminal <t>deoxynucleotidyl</t> transferase <t>dUTP</t> nick end labelling (right hand graphs). All measurements were made on sperm in semen and immediately following density gradient centrifugation. Values show mean ± s.e.m.
    Deoxynucleotidyl Transferase Dutp Nick End Labeling Tunel Assay, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa deoxynucleotidyl transferase dutp nick end labeling tunel assay
    Percentage of sperm with DNA fragmentation in male partners of women with recurrent implantation failure ( a and b ), recurrent miscarriage ( c and d ), and in a group of recent fathers (control) ( e and f ) as measured by sperm chromatin disruption test (left hand graphs) and terminal <t>deoxynucleotidyl</t> transferase <t>dUTP</t> nick end labelling (right hand graphs). All measurements were made on sperm in semen and immediately following density gradient centrifugation. Values show mean ± s.e.m.
    Deoxynucleotidyl Transferase Dutp Nick End Labeling Tunel Assay, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega deadend colorimetric tunel system
    Percentage of sperm with DNA fragmentation in male partners of women with recurrent implantation failure ( a and b ), recurrent miscarriage ( c and d ), and in a group of recent fathers (control) ( e and f ) as measured by sperm chromatin disruption test (left hand graphs) and terminal <t>deoxynucleotidyl</t> transferase <t>dUTP</t> nick end labelling (right hand graphs). All measurements were made on sperm in semen and immediately following density gradient centrifugation. Values show mean ± s.e.m.
    Deadend Colorimetric Tunel System, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Autophagy impairs the function of ASPP2 on inducing apoptosis. Huh7.5 cells were treated with autophagy inhibitor 3-MA and transfected with ASPP2-p. (A) Immunofluorescence assay was used to detect the effect of 3-MA on inhibiting ASPP2-induced autophagy. (B) TUNEL and Calcein AM/PI assays were used to detect the effect of autophagy inhibition via 3-MA on ASPP2-induced apoptosis and cell death. The values represent the mean ± standard deviation of three independent experiments. ASPP2, apoptosis-stimulating protein of p53; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; AM, acetoxymethyl ester; PI, propidium iodide; 3-MA, 3-methyladenosine; ASPP2-p, ASPP2 plasmid.

    Journal: Oncology Letters

    Article Title: DNA damage regulated autophagy modulator 1 recovers the function of apoptosis-stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells

    doi: 10.3892/ol.2018.8453

    Figure Lengend Snippet: Autophagy impairs the function of ASPP2 on inducing apoptosis. Huh7.5 cells were treated with autophagy inhibitor 3-MA and transfected with ASPP2-p. (A) Immunofluorescence assay was used to detect the effect of 3-MA on inhibiting ASPP2-induced autophagy. (B) TUNEL and Calcein AM/PI assays were used to detect the effect of autophagy inhibition via 3-MA on ASPP2-induced apoptosis and cell death. The values represent the mean ± standard deviation of three independent experiments. ASPP2, apoptosis-stimulating protein of p53; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; AM, acetoxymethyl ester; PI, propidium iodide; 3-MA, 3-methyladenosine; ASPP2-p, ASPP2 plasmid.

    Article Snippet: Briefly, calcein-AM (1 µg/ml) and PI (1 µg/ml) were added into the supernatant, and the cells were incubated with calcein-AM/PI for 15 min. Apoptosis was detected using the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay (Promega Corporation, Madison, WI, USA) and the slides were mounted with 50% glycerol, as described previously ( ).

    Techniques: Transfection, Immunofluorescence, TUNEL Assay, Inhibition, Standard Deviation, Plasmid Preparation

    Overexpression of DRAM recovered the pro-apoptotic function of ASPP2 in Huh7.5 cells. Huh7.5 cells were transfected with plasmids encoding ASPP2 or DRAM, or co-transfected with the two plasmids for 48 h. (A) Western blotting detection of overexpression of ASPP2 and DRAM. (B) TUNEL (left panel) and Calcein AM/PI (right panel) assays were used to detect apoptosis and cell death, respectively. The values represent the mean ± standard deviation of three independent experiments. ASPP2, apoptosis-stimulating protein of p53; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; AM, acetoxymethyl ester; PI, propidium iodide; DRAM, DNA damage regulated autophagy modulator 1.

    Journal: Oncology Letters

    Article Title: DNA damage regulated autophagy modulator 1 recovers the function of apoptosis-stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells

    doi: 10.3892/ol.2018.8453

    Figure Lengend Snippet: Overexpression of DRAM recovered the pro-apoptotic function of ASPP2 in Huh7.5 cells. Huh7.5 cells were transfected with plasmids encoding ASPP2 or DRAM, or co-transfected with the two plasmids for 48 h. (A) Western blotting detection of overexpression of ASPP2 and DRAM. (B) TUNEL (left panel) and Calcein AM/PI (right panel) assays were used to detect apoptosis and cell death, respectively. The values represent the mean ± standard deviation of three independent experiments. ASPP2, apoptosis-stimulating protein of p53; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; AM, acetoxymethyl ester; PI, propidium iodide; DRAM, DNA damage regulated autophagy modulator 1.

    Article Snippet: Briefly, calcein-AM (1 µg/ml) and PI (1 µg/ml) were added into the supernatant, and the cells were incubated with calcein-AM/PI for 15 min. Apoptosis was detected using the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay (Promega Corporation, Madison, WI, USA) and the slides were mounted with 50% glycerol, as described previously ( ).

    Techniques: Over Expression, Transfection, Western Blot, TUNEL Assay, Standard Deviation

    ASPP2 overexpression fails to induce apoptotic cell death in Huh7.5 cells. (A) TUNEL and (B) Calcein AM/PI were used to detect the levels of apoptosis and cell death in Huh7.5 cells transfected with ASPP2-p for 48 h, respectively. The values represent the mean ± standard deviation of three independent experiments. ASPP2, apoptosis-stimulating protein of p53; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; AM, acetoxymethyl ester; PI, propidium iodide

    Journal: Oncology Letters

    Article Title: DNA damage regulated autophagy modulator 1 recovers the function of apoptosis-stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells

    doi: 10.3892/ol.2018.8453

    Figure Lengend Snippet: ASPP2 overexpression fails to induce apoptotic cell death in Huh7.5 cells. (A) TUNEL and (B) Calcein AM/PI were used to detect the levels of apoptosis and cell death in Huh7.5 cells transfected with ASPP2-p for 48 h, respectively. The values represent the mean ± standard deviation of three independent experiments. ASPP2, apoptosis-stimulating protein of p53; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; AM, acetoxymethyl ester; PI, propidium iodide

    Article Snippet: Briefly, calcein-AM (1 µg/ml) and PI (1 µg/ml) were added into the supernatant, and the cells were incubated with calcein-AM/PI for 15 min. Apoptosis was detected using the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay (Promega Corporation, Madison, WI, USA) and the slides were mounted with 50% glycerol, as described previously ( ).

    Techniques: Over Expression, TUNEL Assay, Transfection, Standard Deviation

    PAb induced apoptosis in vivo as revealed by TUNEL assay. a – c Sections from the tumour-bearing mice treated with NS, control IgG, or PAb, were stained with FITC-dUTP as described in materials and methods (×200). d An apparent increase in the number of apoptotic cells and apoptotic index was observed within the remaining tumours tissue of mice treated with PAb compared to mice injected subcutaneously with control IgG, *represents significant difference between PAb treatment group mice with the NS group and control IgG group mice ( P

    Journal: Apoptosis

    Article Title: Polyclonal rabbit anti-murine plasmacytoma cell globulins induce myeloma cells apoptosis and inhibit tumour growth in mice

    doi: 10.1007/s10495-010-0568-7

    Figure Lengend Snippet: PAb induced apoptosis in vivo as revealed by TUNEL assay. a – c Sections from the tumour-bearing mice treated with NS, control IgG, or PAb, were stained with FITC-dUTP as described in materials and methods (×200). d An apparent increase in the number of apoptotic cells and apoptotic index was observed within the remaining tumours tissue of mice treated with PAb compared to mice injected subcutaneously with control IgG, *represents significant difference between PAb treatment group mice with the NS group and control IgG group mice ( P

    Article Snippet: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay Cell apoptosis in vivo was investigated using a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay according to the manufacturer’s instructions (Promega, USA).

    Techniques: In Vivo, TUNEL Assay, Mouse Assay, Staining, Injection

    PML immunofluorescence combined with Tel-FISH in U2OS, CM and QGP cell lines. U2OS cell line represents a universal positive control for ALT mechanism. CM cell line, a TERTp wild-type cell line, presented a high co-localization of telomeric DNA with PML exhibiting an ALT positive phenotype. QGP1, the TERTp mutated cell line does not present frequent co-localization of telomeric DNA with being ALT negative. These findings recapitulate the observations in our series of PETs.

    Journal: Scientific Reports

    Article Title: TERT promoter mutations in pancreatic endocrine tumours are rare and mainly found in tumours from patients with hereditary syndromes

    doi: 10.1038/srep29714

    Figure Lengend Snippet: PML immunofluorescence combined with Tel-FISH in U2OS, CM and QGP cell lines. U2OS cell line represents a universal positive control for ALT mechanism. CM cell line, a TERTp wild-type cell line, presented a high co-localization of telomeric DNA with PML exhibiting an ALT positive phenotype. QGP1, the TERTp mutated cell line does not present frequent co-localization of telomeric DNA with being ALT negative. These findings recapitulate the observations in our series of PETs.

    Article Snippet: DNA oligonucleotides corresponding to the −124 and −146 regions of the TERTp were synthesized and labelled with biotin using the Biotin 3′ End DNA Labelling Kit (Thermo Scientific).

    Techniques: Immunofluorescence, Fluorescence In Situ Hybridization, Positive Control

    Increased apoptotic cell death following overventilation (OV) in lungs lacking TRIM72 and/or Cav1. A : representative terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining on lung tissue at ×100; WT, TRIM72 KO , Cav1 KO , and

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: TRIM72 modulates caveolar endocytosis in repair of lung cells

    doi: 10.1152/ajplung.00089.2015

    Figure Lengend Snippet: Increased apoptotic cell death following overventilation (OV) in lungs lacking TRIM72 and/or Cav1. A : representative terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining on lung tissue at ×100; WT, TRIM72 KO , Cav1 KO , and

    Article Snippet: Unstained paraffin sections (5 μm) from ventilated lungs were assayed for apoptosis using the DeadEnd fluorometric terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) system (Promega, Madison, WI), according to the manufacturer's instructions.

    Techniques: TUNEL Assay, Staining

    Percentage of sperm with DNA fragmentation in male partners of women with recurrent implantation failure ( a and b ), recurrent miscarriage ( c and d ), and in a group of recent fathers (control) ( e and f ) as measured by sperm chromatin disruption test (left hand graphs) and terminal deoxynucleotidyl transferase dUTP nick end labelling (right hand graphs). All measurements were made on sperm in semen and immediately following density gradient centrifugation. Values show mean ± s.e.m.

    Journal: Asian Journal of Andrology

    Article Title: Sperm DNA fragmentation, recurrent implantation failure and recurrent miscarriage

    doi: 10.4103/1008-682X.144946

    Figure Lengend Snippet: Percentage of sperm with DNA fragmentation in male partners of women with recurrent implantation failure ( a and b ), recurrent miscarriage ( c and d ), and in a group of recent fathers (control) ( e and f ) as measured by sperm chromatin disruption test (left hand graphs) and terminal deoxynucleotidyl transferase dUTP nick end labelling (right hand graphs). All measurements were made on sperm in semen and immediately following density gradient centrifugation. Values show mean ± s.e.m.

    Article Snippet: The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed using the Fluorescein FragEL™ DNA Fragmentation Detection Kit (Merck Chemicals Ltd., Nottingham, UK).

    Techniques: Gradient Centrifugation