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rea794  (Miltenyi Biotec)
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Miltenyi Biotec rea794
Rea794, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m san nuclease  (ArcticZymes)
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ArcticZymes m san nuclease
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anti cd105 apc  (Miltenyi Biotec)
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Miltenyi Biotec anti cd105 apc
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protein eb1  (ProSci Incorporated)
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ProSci Incorporated protein eb1
Figure 4. Dose-dependent depletion of KSP from monopolar spindles in 1 and 5-treated cells. HeLa cells were incubated in either 1 (A–I) or 5 (J–R) for 4 h and the fixed and processed for KSP (green), the microtubule-binding protein <t>EB1</t> (red) and DNA (blue) localization, and images were acquired using equivalent exposure times for the green channel.
Protein Eb1, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endtag dna end labeling 33 kit  (Vector Laboratories)
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Vector Laboratories endtag dna end labeling 33 kit
Figure 4. Dose-dependent depletion of KSP from monopolar spindles in 1 and 5-treated cells. HeLa cells were incubated in either 1 (A–I) or 5 (J–R) for 4 h and the fixed and processed for KSP (green), the microtubule-binding protein <t>EB1</t> (red) and DNA (blue) localization, and images were acquired using equivalent exposure times for the green channel.
Endtag Dna End Labeling 33 Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human advanced glycation end  (Cusabio)
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Cusabio human advanced glycation end
Figure 4. Dose-dependent depletion of KSP from monopolar spindles in 1 and 5-treated cells. HeLa cells were incubated in either 1 (A–I) or 5 (J–R) for 4 h and the fixed and processed for KSP (green), the microtubule-binding protein <t>EB1</t> (red) and DNA (blue) localization, and images were acquired using equivalent exposure times for the green channel.
Human Advanced Glycation End, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x end repair buffer  (New England Biolabs)
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New England Biolabs 1x end repair buffer
Figure 4. Dose-dependent depletion of KSP from monopolar spindles in 1 and 5-treated cells. HeLa cells were incubated in either 1 (A–I) or 5 (J–R) for 4 h and the fixed and processed for KSP (green), the microtubule-binding protein <t>EB1</t> (red) and DNA (blue) localization, and images were acquired using equivalent exposure times for the green channel.
1x End Repair Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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double strand specific dnase treatment  (ArcticZymes)
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ArcticZymes double strand specific dnase treatment
Figure 4. Dose-dependent depletion of KSP from monopolar spindles in 1 and 5-treated cells. HeLa cells were incubated in either 1 (A–I) or 5 (J–R) for 4 h and the fixed and processed for KSP (green), the microtubule-binding protein <t>EB1</t> (red) and DNA (blue) localization, and images were acquired using equivalent exposure times for the green channel.
Double Strand Specific Dnase Treatment, supplied by ArcticZymes, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paired end reagent kit  (Illumina Inc)
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Illumina Inc paired end reagent kit
Figure 4. Dose-dependent depletion of KSP from monopolar spindles in 1 and 5-treated cells. HeLa cells were incubated in either 1 (A–I) or 5 (J–R) for 4 h and the fixed and processed for KSP (green), the microtubule-binding protein <t>EB1</t> (red) and DNA (blue) localization, and images were acquired using equivalent exposure times for the green channel.
Paired End Reagent Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kit  (Cusabio)
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Cusabio elisa kit
Figure 4. Dose-dependent depletion of KSP from monopolar spindles in 1 and 5-treated cells. HeLa cells were incubated in either 1 (A–I) or 5 (J–R) for 4 h and the fixed and processed for KSP (green), the microtubule-binding protein <t>EB1</t> (red) and DNA (blue) localization, and images were acquired using equivalent exposure times for the green channel.
Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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instant sticky end ligase 2× master mix  (New England Biolabs)
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New England Biolabs instant sticky end ligase 2× master mix
Figure 4. Dose-dependent depletion of KSP from monopolar spindles in 1 and 5-treated cells. HeLa cells were incubated in either 1 (A–I) or 5 (J–R) for 4 h and the fixed and processed for KSP (green), the microtubule-binding protein <t>EB1</t> (red) and DNA (blue) localization, and images were acquired using equivalent exposure times for the green channel.
Instant Sticky End Ligase 2× Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext end repair module  (New England Biolabs)
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New England Biolabs nebnext end repair module
Figure 4. Dose-dependent depletion of KSP from monopolar spindles in 1 and 5-treated cells. HeLa cells were incubated in either 1 (A–I) or 5 (J–R) for 4 h and the fixed and processed for KSP (green), the microtubule-binding protein <t>EB1</t> (red) and DNA (blue) localization, and images were acquired using equivalent exposure times for the green channel.
Nebnext End Repair Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 4. Dose-dependent depletion of KSP from monopolar spindles in 1 and 5-treated cells. HeLa cells were incubated in either 1 (A–I) or 5 (J–R) for 4 h and the fixed and processed for KSP (green), the microtubule-binding protein EB1 (red) and DNA (blue) localization, and images were acquired using equivalent exposure times for the green channel.

Journal: Bioorganic & Medicinal Chemistry

Article Title: Synthesis and characterization of tritylthioethanamine derivatives with potent KSP inhibitory activity

doi: 10.1016/j.bmc.2011.07.054

Figure Lengend Snippet: Figure 4. Dose-dependent depletion of KSP from monopolar spindles in 1 and 5-treated cells. HeLa cells were incubated in either 1 (A–I) or 5 (J–R) for 4 h and the fixed and processed for KSP (green), the microtubule-binding protein EB1 (red) and DNA (blue) localization, and images were acquired using equivalent exposure times for the green channel.

Article Snippet: Cells were then probed with either mouse anti-tubulin (Sigma, Co, St. Louis, MO), or mouse anti-KSP (Abcam, Cambridge, MA) in blocking buffer overnight at 4 C. To counterstain the microtubule cytoskeleton in KSP localization experiments, cells were also probed for the presence of the microtubule end-binding protein EB1 using a custom rabbit polyclonal antibody against whole recombinant human EB1 (Pro-Sci, Poway, CA).

Techniques: Incubation, Binding Assay