empty vector expression plasmid Search Results


95
Addgene inc taggcaccatggtccactgcag mythdf1 e4
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Addgene inc lentiviral gfp cre empty vector
(A-F) Wild type (A- B), Nlrp3 L351P/L351P (C-D) or Nlrp3 A350V/A350V (E-F) BMDMs that were transduced with a Cre-recombinase expressing <t>lentiviral</t> vector, were left unstimulated (Ctrl) or stimulated with LPS and then left untreated or treated with ATP or nigericin (Nig) in absence or presence of MCC950/CRID3. Supernatants was analyzed for IL-1β (A,C,E) and IL-18 (B,D,F) secretion. Graphs show mean ± s.d. of triplicate wells and represent three independent experiments.
Lentiviral Gfp Cre Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc puro cre empty vector
(A-F) Wild type (A- B), Nlrp3 L351P/L351P (C-D) or Nlrp3 A350V/A350V (E-F) BMDMs that were transduced with a Cre-recombinase expressing <t>lentiviral</t> vector, were left unstimulated (Ctrl) or stimulated with LPS and then left untreated or treated with ATP or nigericin (Nig) in absence or presence of MCC950/CRID3. Supernatants was analyzed for IL-1β (A,C,E) and IL-18 (B,D,F) secretion. Graphs show mean ± s.d. of triplicate wells and represent three independent experiments.
Puro Cre Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc luciferase vector ori empty plasmid
(A-F) Wild type (A- B), Nlrp3 L351P/L351P (C-D) or Nlrp3 A350V/A350V (E-F) BMDMs that were transduced with a Cre-recombinase expressing <t>lentiviral</t> vector, were left unstimulated (Ctrl) or stimulated with LPS and then left untreated or treated with ATP or nigericin (Nig) in absence or presence of MCC950/CRID3. Supernatants was analyzed for IL-1β (A,C,E) and IL-18 (B,D,F) secretion. Graphs show mean ± s.d. of triplicate wells and represent three independent experiments.
Luciferase Vector Ori Empty Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc luc cre empty vector
(A-F) Wild type (A- B), Nlrp3 L351P/L351P (C-D) or Nlrp3 A350V/A350V (E-F) BMDMs that were transduced with a Cre-recombinase expressing <t>lentiviral</t> vector, were left unstimulated (Ctrl) or stimulated with LPS and then left untreated or treated with ATP or nigericin (Nig) in absence or presence of MCC950/CRID3. Supernatants was analyzed for IL-1β (A,C,E) and IL-18 (B,D,F) secretion. Graphs show mean ± s.d. of triplicate wells and represent three independent experiments.
Luc Cre Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc pet his6 sumo vector
(A-F) Wild type (A- B), Nlrp3 L351P/L351P (C-D) or Nlrp3 A350V/A350V (E-F) BMDMs that were transduced with a Cre-recombinase expressing <t>lentiviral</t> vector, were left unstimulated (Ctrl) or stimulated with LPS and then left untreated or treated with ATP or nigericin (Nig) in absence or presence of MCC950/CRID3. Supernatants was analyzed for IL-1β (A,C,E) and IL-18 (B,D,F) secretion. Graphs show mean ± s.d. of triplicate wells and represent three independent experiments.
Pet His6 Sumo Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc gall 10 his6 mbp tev ura s cerevisiae expression vector
(A-F) Wild type (A- B), Nlrp3 L351P/L351P (C-D) or Nlrp3 A350V/A350V (E-F) BMDMs that were transduced with a Cre-recombinase expressing <t>lentiviral</t> vector, were left unstimulated (Ctrl) or stimulated with LPS and then left untreated or treated with ATP or nigericin (Nig) in absence or presence of MCC950/CRID3. Supernatants was analyzed for IL-1β (A,C,E) and IL-18 (B,D,F) secretion. Graphs show mean ± s.d. of triplicate wells and represent three independent experiments.
Gall 10 His6 Mbp Tev Ura S Cerevisiae Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A-F) Wild type (A- B), Nlrp3 L351P/L351P (C-D) or Nlrp3 A350V/A350V (E-F) BMDMs that were transduced with a Cre-recombinase expressing lentiviral vector, were left unstimulated (Ctrl) or stimulated with LPS and then left untreated or treated with ATP or nigericin (Nig) in absence or presence of MCC950/CRID3. Supernatants was analyzed for IL-1β (A,C,E) and IL-18 (B,D,F) secretion. Graphs show mean ± s.d. of triplicate wells and represent three independent experiments.

Journal: bioRxiv

Article Title: MCC950/CRID3 potently targets the NACHT domain of wildtype NLRP3 but not disease-associated mutants for inflammasome inhibition

doi: 10.1101/634493

Figure Lengend Snippet: (A-F) Wild type (A- B), Nlrp3 L351P/L351P (C-D) or Nlrp3 A350V/A350V (E-F) BMDMs that were transduced with a Cre-recombinase expressing lentiviral vector, were left unstimulated (Ctrl) or stimulated with LPS and then left untreated or treated with ATP or nigericin (Nig) in absence or presence of MCC950/CRID3. Supernatants was analyzed for IL-1β (A,C,E) and IL-18 (B,D,F) secretion. Graphs show mean ± s.d. of triplicate wells and represent three independent experiments.

Article Snippet: To induce lentivirus production, the lentiviral GFP.Cre empty vector (20781, Addgene) together with 2 nd generation packaging vector psPAX2 (12260, Addgene) and VSV-G-expressing envelope plasmid pCMV-VSV-G (8454, Addgene) were transfected into HEK293 T cells using jetPRIME transfection reagent (114-15, PolyPlus Transfect).

Techniques: Transduction, Expressing, Plasmid Preparation