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Image Search Results
Journal: Nature Communications
Article Title: Identification of limb-specific Lmx1b auto-regulatory modules with Nail-patella syndrome pathogenicity
doi: 10.1038/s41467-021-25844-5
Figure Lengend Snippet: a Human LARM constructs electroporated into chick wing buds display dorsally restricted expression ( hLARM1 , n = 13; hLARM2 , n = 7; hLARM1/2 region , n = 3). b Similarly, transgenic mice containing the human LARM sequences linked to a LacZ reporter demonstrate dorsally accentuated activity. All three LARM1 transgenic embryos display limb-restricted, dorsally accentuated activity. LARM2 transgenic embryos exhibit tight dorsally restricted activity in the limb (5/5). LacZ staining is reduced-to-absent in the posterior distal autopod mesoderm (5th digit region, yellow arrow). Transgenic embryos containing both hLARM1/2 (~8 kb, hLARM1/2 ) also show activity restricted to the dorsal limb (7/7). However, focal ventral activity at the zeugopod/autopod junction is also evident (yellow arrowhead).
Article Snippet: The constructs were used to generate
Techniques: Construct, Expressing, Transgenic Assay, Activity Assay, Staining