Journal: Aging (Albany NY)
Article Title: Tanshinone IIA attenuates atherosclerosis via inhibiting NLRP3 inflammasome activation
Figure Lengend Snippet: Tan IIA inhibits oxLDL-induced CD36 expression by inhibiting Nrf2 expression and PPARγ activity. ( A , B ) Effects of oxLDL on CD36 expressions at mRNA levels measured by qRT-PCR and protein levels detected by Western blot in macrophage, respectively; GAPDH, glyceraldehyde-3-phosphate dehydrogenase as an internal control. ( C , D ) Effects of Tan IIA on oxLDL-induced CD36 expressions at mRNA levels and protein levels in macrophages, respectively. ( E ) Effects of Tan IIA on oxLDL-induced caspase-1 activation in the cells detected by Western blot. ( F ) Effects of Tan IIA on oxLDL-induced IL-1β productions measured by ELISA in macrophages. ( G , H ) Effects of Nrf2 and PPARγ gene knockdown by siRNA on CD36 expression on the cell membrane, respectively, detected by flow cytometry, presented by mean fluorescence intensity (MFI). ( I , J ) Effects of Tan IIA on oxLDL-mediated Nrf2 expression measured by RT-PCR and oxLDL-mediated PPARγ activity measured by a commercial kit in the cells.
Article Snippet: The measurements of all the above cytokines, except for IL-1β, were performed using commercially available ELISA kits (R & D Systems) according to the manufactures’ instructions.
Techniques: Expressing, Activity Assay, Quantitative RT-PCR, Western Blot, Activation Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Fluorescence, Reverse Transcription Polymerase Chain Reaction