elisa enzyme-linked immunosorbent assay methodology Search Results


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  • 99
    Thermo Fisher elisa method
    Mean (±SEM) lung keratinocyte–derived chemokine (KC), <t>TNF-α,</t> and IL-10 levels 6 and 24 hours after intratracheal administration of P. aeruginosa . In ( A ) and ( B ), mice were infected with P. aeruginosa at a dose of 1.1 × 10 6 CFU. BALFs were collected at 6 hours after infection and measured for KC and TNF-α expression by <t>ELISA</t> with determinations made in triplicate. In ( C ) and ( D ), BALFs were collected at 24 hours after infection and measured for KC and TNF-α expression. ( E ) Endogenous IL-10 (mouse IL-10) and exogenous IL-10 (human IL-10) levels were measured by ELISA in a similar manner from 6- and 24-hour BALF samples. * P
    Elisa Method, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore elisa procedures
    Prevalence of <t>AAV-binding</t> antibodies in sera collected from four different cat populations comprising a total of 99 cats living in the Northeastern United States. ( a ) Box plots showing levels of IgG antibodies in cat serum samples that bind to each AAV serotype capsid in each group: Group 1, 35 client-owned cats; Group 2, 20 feral cats; Group 3, 30 SPF cats that had received FPV vaccine; and Group 4 Pre-vac, 14 SPF cats that had not received FPV vaccine. The quantities of AAV-binding IgG antibodies were determined by AAV-binding antibody <t>ELISA</t> for each serotype using 1:5-diluted serum samples and expressed as ODc. The values indicate averages of technical duplicates. (b) Selection of an optimal number of clusters used for a k-means clustering analysis of a total of 99 cats. The graph shows within-cluster sum of squares (WSS) values as a function of the number of clusters (k). We selected an elbow point at k = 5, indicated with a dotted line, as an optimal k for clustering. The k-means clustering identified 5 clusters, Clusters Ak to Ek (Supplementary Table S1 ). (c ) A t-SNE plot showing the distribution of cats from 4 different populations (Group 1, red; Group 2, green; Group 3, purple; and Group 4 Pre-vac, cyan). They form 5 distinct clusters (Clusters Ak to Ek, Supplementary Table S1 ) based on the AAV-binding antibody spectra. (d , e) Violin plots showing AAV-binding antibody levels for each serotype in each cluster identified by k-means clustering ( d ) and visualized by t-SNE ( e ).
    Elisa Procedures, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher bicinchoninic acid method
    Changes in the NLRP3 inflammasome-associated molecules and cytokines during oxazolone-induced colitis. ( A – H ) mRNA levels of NLRP3 ( A ), caspase-1 ( B ), IL-1β ( C ), IL-18 ( D ), INF-γ ( E ), TNF-α ( F ), IL-4 ( G ), and IL-13 ( H ) in oxazolone-induced mice colonic tissue. The levels of indicated mRNA were determined by quantitative reverse transcription-polymerase chain reaction. mRNA levels are expressed as ratios, relative to the mean value for normal colonic tissue. ( I , J ) Protein concentration of IL-4 ( I ) and IL-13 ( J ) in oxazolone-induced mice colonic tissue. Protein concentrations were measured by the corresponding enzyme-linked immunosorbent assay. The protein levels in each sample were standardized using the modified <t>bicinchoninic</t> acid method. Each box plot represents median, 10–90% range, minimum, and maximum values. p values were calculated by post hoc steel significant difference multiple comparison. N = 9. **p
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    93
    ALPCO elisa method
    Changes in the NLRP3 inflammasome-associated molecules and cytokines during oxazolone-induced colitis. ( A – H ) mRNA levels of NLRP3 ( A ), caspase-1 ( B ), IL-1β ( C ), IL-18 ( D ), INF-γ ( E ), TNF-α ( F ), IL-4 ( G ), and IL-13 ( H ) in oxazolone-induced mice colonic tissue. The levels of indicated mRNA were determined by quantitative reverse transcription-polymerase chain reaction. mRNA levels are expressed as ratios, relative to the mean value for normal colonic tissue. ( I , J ) Protein concentration of IL-4 ( I ) and IL-13 ( J ) in oxazolone-induced mice colonic tissue. Protein concentrations were measured by the corresponding enzyme-linked immunosorbent assay. The protein levels in each sample were standardized using the modified <t>bicinchoninic</t> acid method. Each box plot represents median, 10–90% range, minimum, and maximum values. p values were calculated by post hoc steel significant difference multiple comparison. N = 9. **p
    Elisa Method, supplied by ALPCO, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Abcam elisa method
    Antigen dose curves of antigen specific IgG, <t>IgG1</t> and IgG2c responses induced by influenza antigen and with or without 1.5 μg of QS-21 by the needle and syringe intramuscularly (IM). IM groups were represented by and (unadjuvanted and with 1.5 μg of QS-21 ). ( a ) Total antigen specific IgG ( b ) IgG1 and ( c ) IgG2c induced by co-administering of influenza antigen different doses (6, 60, 600, and 6000 ng) unadjuvanted or with 1.5 μg of QS-21 by IM, 21 days post immunisation. <t>ELISA</t> antibody data represent the Mean ± SEM, statistical significance is when p
    Elisa Method, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Innogenetics sandwich elisa method
    HMGB1 released by HSV-2-infected cells activates fibroblast migration and stimulates <t>HIV-1</t> expression. MRC5 fibroblasts were subjected to migration assays in the presence of (A) human recombinant HMGB1 or (B) supernatants from mock (M) or HSV-2-infected HEC-1 cells. HMGB1 concentrations reached 270 ng/ml in the supernatants collected from infected cells, versus 50 ng/ml in mock-infected cell media. Control experiments used glycyrrhizin or neutralizing antibodies against HMGB1 or its receptors RAGE, TLR-4 and TLR-2. Data are expressed as -fold increases in cell migration compared to non treated control cells. ACH-2 cells, that contain a latent HIV-1 provirus, were grown in the presence of (C) human recombinant HMGB1 or (D) supernatants from mock (M) or HSV-2-infected HEC-1 cells. P24 antigen was measured by <t>ELISA</t> after 36 h. Data are expressed as the -fold increase in p24 antigen compared with non treated control cells. Virion-free supernatants were obtained by ultracentrifugation. Results are either representative of several experiments (A, C, D) or expressed as the mean and SD of 3 independent experiments (B).
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    92
    Bio-Rad elisa method
    HMGB1 released by HSV-2-infected cells activates fibroblast migration and stimulates <t>HIV-1</t> expression. MRC5 fibroblasts were subjected to migration assays in the presence of (A) human recombinant HMGB1 or (B) supernatants from mock (M) or HSV-2-infected HEC-1 cells. HMGB1 concentrations reached 270 ng/ml in the supernatants collected from infected cells, versus 50 ng/ml in mock-infected cell media. Control experiments used glycyrrhizin or neutralizing antibodies against HMGB1 or its receptors RAGE, TLR-4 and TLR-2. Data are expressed as -fold increases in cell migration compared to non treated control cells. ACH-2 cells, that contain a latent HIV-1 provirus, were grown in the presence of (C) human recombinant HMGB1 or (D) supernatants from mock (M) or HSV-2-infected HEC-1 cells. P24 antigen was measured by <t>ELISA</t> after 36 h. Data are expressed as the -fold increase in p24 antigen compared with non treated control cells. Virion-free supernatants were obtained by ultracentrifugation. Results are either representative of several experiments (A, C, D) or expressed as the mean and SD of 3 independent experiments (B).
    Elisa Method, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Cayman Chemical elisa method
    Real-time PCR assessment of <t>PPARγ</t> gene expression in different human cell lines (A) and analysis of DNA-binding activity of PPARγ in nuclear extracts of human BSMCs by <t>ELISA</t> (B). C+, positive control; Sfm, serum-free medium; Rgz, rosiglitazone. Data are expressed as mean ± SEM. *** P
    Elisa Method, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    RayBiotech tgf β
    Effects of MitoQ, MSU, TRX siRNA and TXNIP siRNA on TXNIP/NLRP3/IL-1β axis and pro-fibrotic protein expression and apoptosis in HK-2 cells cultured under HG conditions . A : IF showing FN ( Aa-c ) and Collagen I ( Ad-f ) expression in HK-2 cells subjected to HG treatment following MitoQ treatment. B: WB analysis of Caspase-1, cleaved IL-1β and IL-18 expression in HK-2 cells cultured under HG conditions and treated with different concentrations of MitoQ (50,100, 150 nM). C-H: ELISA of Caspase-1 ( C ), IL-1β ( D ), IL-18 ( E ), <t>TGF-β</t> ( F ) activity; TGF-β expression ( G ); and relative apoptosis ( H ) in HK-2 cells subjected to HG conditions and treated with different concentrations of MitoQ (50, 100, 150 nM). I: Luminex analysis of IL-1β, IL-18, FN and Collagen I expression in HK-2 cells subjected to an HG environment and treated with MitoQ, MSU or TXNIP siRNA. J, K: WB and densitometric analysis of NLRP3, IL-1β, IL-18 and FN expression in HK-2 cells exposed to an HG environment and pretreated with MitoQ, TRX siRNA or TXNIP siRNA. Values are the mean ± SE; *P
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    92
    Bender MedSystems elisa method
    Effects of MitoQ, MSU, TRX siRNA and TXNIP siRNA on TXNIP/NLRP3/IL-1β axis and pro-fibrotic protein expression and apoptosis in HK-2 cells cultured under HG conditions . A : IF showing FN ( Aa-c ) and Collagen I ( Ad-f ) expression in HK-2 cells subjected to HG treatment following MitoQ treatment. B: WB analysis of Caspase-1, cleaved IL-1β and IL-18 expression in HK-2 cells cultured under HG conditions and treated with different concentrations of MitoQ (50,100, 150 nM). C-H: ELISA of Caspase-1 ( C ), IL-1β ( D ), IL-18 ( E ), <t>TGF-β</t> ( F ) activity; TGF-β expression ( G ); and relative apoptosis ( H ) in HK-2 cells subjected to HG conditions and treated with different concentrations of MitoQ (50, 100, 150 nM). I: Luminex analysis of IL-1β, IL-18, FN and Collagen I expression in HK-2 cells subjected to an HG environment and treated with MitoQ, MSU or TXNIP siRNA. J, K: WB and densitometric analysis of NLRP3, IL-1β, IL-18 and FN expression in HK-2 cells exposed to an HG environment and pretreated with MitoQ, TRX siRNA or TXNIP siRNA. Values are the mean ± SE; *P
    Elisa Method, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Biomedica elisa methods
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Elisa Methods, supplied by Biomedica, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    LINCO elisa method
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Elisa Method, supplied by LINCO, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Abcam elisa kit method
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Elisa Kit Method, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Meso Scale Diagnostics LLC electrochemiluminescent detection method
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Electrochemiluminescent Detection Method, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 91/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega enzyme linked immunosorbent assay elisa method
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Enzyme Linked Immunosorbent Assay Elisa Method, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    GE Healthcare elisa method
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Elisa Method, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Immundiagnostik AG enzyme linked immunosorbent assay method
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Enzyme Linked Immunosorbent Assay Method, supplied by Immundiagnostik AG, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Inova Diagnostics elisa methods
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Elisa Methods, supplied by Inova Diagnostics, used in various techniques. Bioz Stars score: 89/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    USCN Life elisa method
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Elisa Method, supplied by USCN Life, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Cayman Chemical eia method
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Eia Method, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 89/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Abbott Laboratories elisa method
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Elisa Method, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Calbiotech elisa procedure
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Elisa Procedure, supplied by Calbiotech, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Merck KGaA enzyme linked immunosorbent assay method
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Enzyme Linked Immunosorbent Assay Method, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MyBiosource elisa method
    The effect of several antioxidants used as competitors in the monoclonal <t>anti-MDA-LDL</t> IgM <t>ELISA.</t> Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.
    Elisa Method, supplied by MyBiosource, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Mean (±SEM) lung keratinocyte–derived chemokine (KC), TNF-α, and IL-10 levels 6 and 24 hours after intratracheal administration of P. aeruginosa . In ( A ) and ( B ), mice were infected with P. aeruginosa at a dose of 1.1 × 10 6 CFU. BALFs were collected at 6 hours after infection and measured for KC and TNF-α expression by ELISA with determinations made in triplicate. In ( C ) and ( D ), BALFs were collected at 24 hours after infection and measured for KC and TNF-α expression. ( E ) Endogenous IL-10 (mouse IL-10) and exogenous IL-10 (human IL-10) levels were measured by ELISA in a similar manner from 6- and 24-hour BALF samples. * P

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: Effect of IL-10 on Neutrophil Recruitment and Survival after Pseudomonas aeruginosa Challenge

    doi: 10.1165/rcmb.2008-0202OC

    Figure Lengend Snippet: Mean (±SEM) lung keratinocyte–derived chemokine (KC), TNF-α, and IL-10 levels 6 and 24 hours after intratracheal administration of P. aeruginosa . In ( A ) and ( B ), mice were infected with P. aeruginosa at a dose of 1.1 × 10 6 CFU. BALFs were collected at 6 hours after infection and measured for KC and TNF-α expression by ELISA with determinations made in triplicate. In ( C ) and ( D ), BALFs were collected at 24 hours after infection and measured for KC and TNF-α expression. ( E ) Endogenous IL-10 (mouse IL-10) and exogenous IL-10 (human IL-10) levels were measured by ELISA in a similar manner from 6- and 24-hour BALF samples. * P

    Article Snippet: Lung KC, TNF-α, mouse IL-10, and human IL-10 levels were measured by the ELISA method (Cytoset; Biosource, Invitrogen, Camarillo, CA) according to the manufacturer's instructions.

    Techniques: Derivative Assay, Mouse Assay, Infection, Expressing, Enzyme-linked Immunosorbent Assay

    Prevalence of AAV-binding antibodies in sera collected from four different cat populations comprising a total of 99 cats living in the Northeastern United States. ( a ) Box plots showing levels of IgG antibodies in cat serum samples that bind to each AAV serotype capsid in each group: Group 1, 35 client-owned cats; Group 2, 20 feral cats; Group 3, 30 SPF cats that had received FPV vaccine; and Group 4 Pre-vac, 14 SPF cats that had not received FPV vaccine. The quantities of AAV-binding IgG antibodies were determined by AAV-binding antibody ELISA for each serotype using 1:5-diluted serum samples and expressed as ODc. The values indicate averages of technical duplicates. (b) Selection of an optimal number of clusters used for a k-means clustering analysis of a total of 99 cats. The graph shows within-cluster sum of squares (WSS) values as a function of the number of clusters (k). We selected an elbow point at k = 5, indicated with a dotted line, as an optimal k for clustering. The k-means clustering identified 5 clusters, Clusters Ak to Ek (Supplementary Table S1 ). (c ) A t-SNE plot showing the distribution of cats from 4 different populations (Group 1, red; Group 2, green; Group 3, purple; and Group 4 Pre-vac, cyan). They form 5 distinct clusters (Clusters Ak to Ek, Supplementary Table S1 ) based on the AAV-binding antibody spectra. (d , e) Violin plots showing AAV-binding antibody levels for each serotype in each cluster identified by k-means clustering ( d ) and visualized by t-SNE ( e ).

    Journal: Scientific Reports

    Article Title: Adeno-associated virus-binding antibodies detected in cats living in the Northeastern United States lack neutralizing activity

    doi: 10.1038/s41598-020-66596-4

    Figure Lengend Snippet: Prevalence of AAV-binding antibodies in sera collected from four different cat populations comprising a total of 99 cats living in the Northeastern United States. ( a ) Box plots showing levels of IgG antibodies in cat serum samples that bind to each AAV serotype capsid in each group: Group 1, 35 client-owned cats; Group 2, 20 feral cats; Group 3, 30 SPF cats that had received FPV vaccine; and Group 4 Pre-vac, 14 SPF cats that had not received FPV vaccine. The quantities of AAV-binding IgG antibodies were determined by AAV-binding antibody ELISA for each serotype using 1:5-diluted serum samples and expressed as ODc. The values indicate averages of technical duplicates. (b) Selection of an optimal number of clusters used for a k-means clustering analysis of a total of 99 cats. The graph shows within-cluster sum of squares (WSS) values as a function of the number of clusters (k). We selected an elbow point at k = 5, indicated with a dotted line, as an optimal k for clustering. The k-means clustering identified 5 clusters, Clusters Ak to Ek (Supplementary Table S1 ). (c ) A t-SNE plot showing the distribution of cats from 4 different populations (Group 1, red; Group 2, green; Group 3, purple; and Group 4 Pre-vac, cyan). They form 5 distinct clusters (Clusters Ak to Ek, Supplementary Table S1 ) based on the AAV-binding antibody spectra. (d , e) Violin plots showing AAV-binding antibody levels for each serotype in each cluster identified by k-means clustering ( d ) and visualized by t-SNE ( e ).

    Article Snippet: These 4 wells, which were coated with the reference cat serum but not with AAV, underwent the same ELISA procedures with Sample Buffer only rather than diluted cat serum-containing Sample Buffer.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Selection

    Changes in the NLRP3 inflammasome-associated molecules and cytokines during oxazolone-induced colitis. ( A – H ) mRNA levels of NLRP3 ( A ), caspase-1 ( B ), IL-1β ( C ), IL-18 ( D ), INF-γ ( E ), TNF-α ( F ), IL-4 ( G ), and IL-13 ( H ) in oxazolone-induced mice colonic tissue. The levels of indicated mRNA were determined by quantitative reverse transcription-polymerase chain reaction. mRNA levels are expressed as ratios, relative to the mean value for normal colonic tissue. ( I , J ) Protein concentration of IL-4 ( I ) and IL-13 ( J ) in oxazolone-induced mice colonic tissue. Protein concentrations were measured by the corresponding enzyme-linked immunosorbent assay. The protein levels in each sample were standardized using the modified bicinchoninic acid method. Each box plot represents median, 10–90% range, minimum, and maximum values. p values were calculated by post hoc steel significant difference multiple comparison. N = 9. **p

    Journal: Scientific Reports

    Article Title: NLRP3 inflammasome has a protective effect against oxazolone-induced colitis: a possible role in ulcerative colitis

    doi: 10.1038/srep39075

    Figure Lengend Snippet: Changes in the NLRP3 inflammasome-associated molecules and cytokines during oxazolone-induced colitis. ( A – H ) mRNA levels of NLRP3 ( A ), caspase-1 ( B ), IL-1β ( C ), IL-18 ( D ), INF-γ ( E ), TNF-α ( F ), IL-4 ( G ), and IL-13 ( H ) in oxazolone-induced mice colonic tissue. The levels of indicated mRNA were determined by quantitative reverse transcription-polymerase chain reaction. mRNA levels are expressed as ratios, relative to the mean value for normal colonic tissue. ( I , J ) Protein concentration of IL-4 ( I ) and IL-13 ( J ) in oxazolone-induced mice colonic tissue. Protein concentrations were measured by the corresponding enzyme-linked immunosorbent assay. The protein levels in each sample were standardized using the modified bicinchoninic acid method. Each box plot represents median, 10–90% range, minimum, and maximum values. p values were calculated by post hoc steel significant difference multiple comparison. N = 9. **p

    Article Snippet: Protein levels in the lysate were measured with the modified bicinchoninic acid method (Thermo Fisher Scientific Inc.).

    Techniques: Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Protein Concentration, Enzyme-linked Immunosorbent Assay, Modification

    Effects of exogenous IL-1β and IL-18 on oxazolone-induced colitis. Mice received intraperitoneal injections of IL-1β (0.1–10 μg/kg), IL-18 (0.1–10 μg/kg), or vehicle after colitis induction. ( A , D ) Colonic histological score in mice given IL-1β ( A ) or IL-18 ( D ). Histological scores were calculated according to the criteria ( Supplementary Table 4 ). B , C , E , and F : mRNA levels of IL-4 ( B , E ) and IL-13 ( C , F ) in oxazolone-induced mice colonic tissue after IL-1β ( B , C ) and IL-18 ( E , F ) administration. The levels of indicated mRNA were determined by quantitative reverse transcription-polymerase chain reaction. mRNA levels are expressed as ratios, relative to the mean value for normal colonic tissue. G – J : Protein concentrations of IL-4 ( G , I ) and IL-13 ( H , J ) in mice given IL-1β ( G , H ) or IL-18 ( I , J ) into OXA-treated mice. Protein concentrations were measured by the corresponding enzyme-linked immunosorbent assay. The protein levels in each sample were standardized using the modified bicinchoninic acid method. Each box plot represents median, 10–90% range, minimum, and maximum values. p values were calculated by post hoc steel significant difference multiple comparison. N = 7–10. **p

    Journal: Scientific Reports

    Article Title: NLRP3 inflammasome has a protective effect against oxazolone-induced colitis: a possible role in ulcerative colitis

    doi: 10.1038/srep39075

    Figure Lengend Snippet: Effects of exogenous IL-1β and IL-18 on oxazolone-induced colitis. Mice received intraperitoneal injections of IL-1β (0.1–10 μg/kg), IL-18 (0.1–10 μg/kg), or vehicle after colitis induction. ( A , D ) Colonic histological score in mice given IL-1β ( A ) or IL-18 ( D ). Histological scores were calculated according to the criteria ( Supplementary Table 4 ). B , C , E , and F : mRNA levels of IL-4 ( B , E ) and IL-13 ( C , F ) in oxazolone-induced mice colonic tissue after IL-1β ( B , C ) and IL-18 ( E , F ) administration. The levels of indicated mRNA were determined by quantitative reverse transcription-polymerase chain reaction. mRNA levels are expressed as ratios, relative to the mean value for normal colonic tissue. G – J : Protein concentrations of IL-4 ( G , I ) and IL-13 ( H , J ) in mice given IL-1β ( G , H ) or IL-18 ( I , J ) into OXA-treated mice. Protein concentrations were measured by the corresponding enzyme-linked immunosorbent assay. The protein levels in each sample were standardized using the modified bicinchoninic acid method. Each box plot represents median, 10–90% range, minimum, and maximum values. p values were calculated by post hoc steel significant difference multiple comparison. N = 7–10. **p

    Article Snippet: Protein levels in the lysate were measured with the modified bicinchoninic acid method (Thermo Fisher Scientific Inc.).

    Techniques: Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Modification

    Effects of NLRP3 deficiency on oxazolone-induced colitis. ( A ) Changes in body weight during the experimental period. Round represents WT, wild-type. ( B , C ) Changes in histological score during experimental period. Histological scores were calculated according to the criteria ( Supplementary Table 4 ). ( D , E ) Histological findings in wild-type ( D ) and NLRP3 −/− mice ( E ) with colitis on day 1. Compared to wild-type mice, NLRP3 −/− mice exhibited severe colitis on day 1. F – I : mRNA expression of IL-1β, IL-18, IL-4, and IL-13. The levels of indicated mRNA were determined by quantitative reverse transcription-polymerase chain reaction. mRNA levels are expressed as ratios, relative to the mean value for normal colonic tissue. ( J , K ) Protein concentrations of IL-4 and IL-13. Protein concentrations were measured by the corresponding enzyme-linked immunosorbent assay. The protein levels in each sample were standardized using the modified bicinchoninic acid method. ( L – Q ) Protein expression of IL-1β and IL-18. Pro- and mature IL-1β and pro- and mature IL-18 were investigated by western blot using its corresponding antibodies ( Supplementary Table 2 ) and relevant bands were quantified with laser-scanning densitometry. β-actin was used as the normalization control. R, S: Changes in the histological score in mice given IL-1β or IL-18. NLRP3 −/− mice received intraperitoneal injections of IL-1β (0.1 μg/kg), IL-18 (0.1 μg/kg), or vehicle after colitis induction. Histological scores were calculated according to the criteria ( Supplementary Table 4 ). Each box plot represents median, 10–90% range, minimum, and maximum values. p values were calculated by post hoc steel significant difference multiple comparison. N = 5–10. **p

    Journal: Scientific Reports

    Article Title: NLRP3 inflammasome has a protective effect against oxazolone-induced colitis: a possible role in ulcerative colitis

    doi: 10.1038/srep39075

    Figure Lengend Snippet: Effects of NLRP3 deficiency on oxazolone-induced colitis. ( A ) Changes in body weight during the experimental period. Round represents WT, wild-type. ( B , C ) Changes in histological score during experimental period. Histological scores were calculated according to the criteria ( Supplementary Table 4 ). ( D , E ) Histological findings in wild-type ( D ) and NLRP3 −/− mice ( E ) with colitis on day 1. Compared to wild-type mice, NLRP3 −/− mice exhibited severe colitis on day 1. F – I : mRNA expression of IL-1β, IL-18, IL-4, and IL-13. The levels of indicated mRNA were determined by quantitative reverse transcription-polymerase chain reaction. mRNA levels are expressed as ratios, relative to the mean value for normal colonic tissue. ( J , K ) Protein concentrations of IL-4 and IL-13. Protein concentrations were measured by the corresponding enzyme-linked immunosorbent assay. The protein levels in each sample were standardized using the modified bicinchoninic acid method. ( L – Q ) Protein expression of IL-1β and IL-18. Pro- and mature IL-1β and pro- and mature IL-18 were investigated by western blot using its corresponding antibodies ( Supplementary Table 2 ) and relevant bands were quantified with laser-scanning densitometry. β-actin was used as the normalization control. R, S: Changes in the histological score in mice given IL-1β or IL-18. NLRP3 −/− mice received intraperitoneal injections of IL-1β (0.1 μg/kg), IL-18 (0.1 μg/kg), or vehicle after colitis induction. Histological scores were calculated according to the criteria ( Supplementary Table 4 ). Each box plot represents median, 10–90% range, minimum, and maximum values. p values were calculated by post hoc steel significant difference multiple comparison. N = 5–10. **p

    Article Snippet: Protein levels in the lysate were measured with the modified bicinchoninic acid method (Thermo Fisher Scientific Inc.).

    Techniques: Mouse Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Modification, Western Blot

    Antigen dose curves of antigen specific IgG, IgG1 and IgG2c responses induced by influenza antigen and with or without 1.5 μg of QS-21 by the needle and syringe intramuscularly (IM). IM groups were represented by and (unadjuvanted and with 1.5 μg of QS-21 ). ( a ) Total antigen specific IgG ( b ) IgG1 and ( c ) IgG2c induced by co-administering of influenza antigen different doses (6, 60, 600, and 6000 ng) unadjuvanted or with 1.5 μg of QS-21 by IM, 21 days post immunisation. ELISA antibody data represent the Mean ± SEM, statistical significance is when p

    Journal: Scientific Reports

    Article Title: Potent response of QS-21 as a vaccine adjuvant in the skin when delivered with the Nanopatch, resulted in adjuvant dose sparing

    doi: 10.1038/srep29368

    Figure Lengend Snippet: Antigen dose curves of antigen specific IgG, IgG1 and IgG2c responses induced by influenza antigen and with or without 1.5 μg of QS-21 by the needle and syringe intramuscularly (IM). IM groups were represented by and (unadjuvanted and with 1.5 μg of QS-21 ). ( a ) Total antigen specific IgG ( b ) IgG1 and ( c ) IgG2c induced by co-administering of influenza antigen different doses (6, 60, 600, and 6000 ng) unadjuvanted or with 1.5 μg of QS-21 by IM, 21 days post immunisation. ELISA antibody data represent the Mean ± SEM, statistical significance is when p

    Article Snippet: IgG1 and IgG2c isotypes responses were assessed using the same ELISA method (IgG1, ab97240; 1:4000, and IgG2c ab97255; 1:2000; Abcam).

    Techniques: Enzyme-linked Immunosorbent Assay

    Adjuvant dose curve of antigen specific IgG, IgG1 and IgG2c responses induced by influenza antigen and co-delivery of different dose of QS-21 or 1.5 μg of QA by Nanopatch. Nanopatch groups were represented by (unadjuvanted , QS-21 ) and (QA ) ( a ) Total antigen specific IgG ( b ) IgG1 and ( c ) IgG2c induced by co-administering 6 ng of influenza antigen with QS-21 at different doses (0, 0.5, 1.5, 3.0 and 6.0 μg) or 1.5 μg QA delivered by the Nanopatch, 21 days post immunisation. ELISA antibody data represent the Mean ± SEM, statistical significance is when p

    Journal: Scientific Reports

    Article Title: Potent response of QS-21 as a vaccine adjuvant in the skin when delivered with the Nanopatch, resulted in adjuvant dose sparing

    doi: 10.1038/srep29368

    Figure Lengend Snippet: Adjuvant dose curve of antigen specific IgG, IgG1 and IgG2c responses induced by influenza antigen and co-delivery of different dose of QS-21 or 1.5 μg of QA by Nanopatch. Nanopatch groups were represented by (unadjuvanted , QS-21 ) and (QA ) ( a ) Total antigen specific IgG ( b ) IgG1 and ( c ) IgG2c induced by co-administering 6 ng of influenza antigen with QS-21 at different doses (0, 0.5, 1.5, 3.0 and 6.0 μg) or 1.5 μg QA delivered by the Nanopatch, 21 days post immunisation. ELISA antibody data represent the Mean ± SEM, statistical significance is when p

    Article Snippet: IgG1 and IgG2c isotypes responses were assessed using the same ELISA method (IgG1, ab97240; 1:4000, and IgG2c ab97255; 1:2000; Abcam).

    Techniques: Enzyme-linked Immunosorbent Assay

    Total serum antigen specific IgG response comparing influenza antigen doses with/without 1.5 μg of QS-21 day 21 day post immunisation delivered by Nanopatch or needle and syringe intramuscular (IM) route. Nanopatch groups were represented by (unadjuvanted , QS-21 ), IM groups were represented by and (unadjuvanted and of QS-21 ). ( a ) Total serum antigen specific IgG was unadjuvanted influenza antigen, delivered by Nanopatch (at 6 ng) and IM (at 6, 60, 600 and 6000 ng). ( b ) Total serum antigen specific IgG was co-delivered with 1.5 μg of QS-21 and influenza antigen, delivered by Nanopatch (at 6 ng) or IM (at 6, 60, 600 and 6000 ng). ( c ) Total serum antigen specific IgG was co-delivered with 1.5 μg of QS-21 and influenza antigen, delivered by Nanopatch (at 6 ng) compared to unadjuvanted IM (at 6, 60, 600 and 6000 ng). ELISA antibody data represent the Mean ± SEM, statistical significance is when p

    Journal: Scientific Reports

    Article Title: Potent response of QS-21 as a vaccine adjuvant in the skin when delivered with the Nanopatch, resulted in adjuvant dose sparing

    doi: 10.1038/srep29368

    Figure Lengend Snippet: Total serum antigen specific IgG response comparing influenza antigen doses with/without 1.5 μg of QS-21 day 21 day post immunisation delivered by Nanopatch or needle and syringe intramuscular (IM) route. Nanopatch groups were represented by (unadjuvanted , QS-21 ), IM groups were represented by and (unadjuvanted and of QS-21 ). ( a ) Total serum antigen specific IgG was unadjuvanted influenza antigen, delivered by Nanopatch (at 6 ng) and IM (at 6, 60, 600 and 6000 ng). ( b ) Total serum antigen specific IgG was co-delivered with 1.5 μg of QS-21 and influenza antigen, delivered by Nanopatch (at 6 ng) or IM (at 6, 60, 600 and 6000 ng). ( c ) Total serum antigen specific IgG was co-delivered with 1.5 μg of QS-21 and influenza antigen, delivered by Nanopatch (at 6 ng) compared to unadjuvanted IM (at 6, 60, 600 and 6000 ng). ELISA antibody data represent the Mean ± SEM, statistical significance is when p

    Article Snippet: IgG1 and IgG2c isotypes responses were assessed using the same ELISA method (IgG1, ab97240; 1:4000, and IgG2c ab97255; 1:2000; Abcam).

    Techniques: Enzyme-linked Immunosorbent Assay

    Total serum antigen specific IgG response comparing QS-21 dose 21 days post immunisation at 1.5 μg delivered by Nanopatch, or 10 μg or 50 μg delivered by the needle and syringe intramuscular (IM) route. Nanopatch groups were represented by (6 ng , 60 ng and 120 ng of influenza antigen) ; IM groups were represented by (10 μg or 50 μg of QS-21 ) with different dose of influenza antigen (6 ng, 60 ng, 120 ng and 600 ng). ( a ) Antigen specific endpoint titres of (i) Nanopatch induced response by 6 ng, 60 ng or 120 ng of influenza antigen without QS-21, (ii) IM induced response by 10 μg of QS-21 with different dose of influenza antigen (6 ng, 60 ng, 120 ng and 600 ng, (iii) IM induced response by 50 μg of QS-21 with different dose of influenza antigen (6 ng, 60 ng, 120 ng and 600 ng; ( b ) Nanopatch and IM induce antigen specific IgG from different dose of QS-21 (1.5 μg, 10 μg and 50 μg) and ( c ) Haemagglutination Inhibition (HI) titres of one of the influenza strain A/Victoria/361/2011 (H3N2) from Nanopatch and IM at 60 ng and 120 ng of antigen with 1.5 μg, 10 μg and 50 μg of QS-21. ELISA antibody data represent the Mean ± SEM, statistical significance is when p

    Journal: Scientific Reports

    Article Title: Potent response of QS-21 as a vaccine adjuvant in the skin when delivered with the Nanopatch, resulted in adjuvant dose sparing

    doi: 10.1038/srep29368

    Figure Lengend Snippet: Total serum antigen specific IgG response comparing QS-21 dose 21 days post immunisation at 1.5 μg delivered by Nanopatch, or 10 μg or 50 μg delivered by the needle and syringe intramuscular (IM) route. Nanopatch groups were represented by (6 ng , 60 ng and 120 ng of influenza antigen) ; IM groups were represented by (10 μg or 50 μg of QS-21 ) with different dose of influenza antigen (6 ng, 60 ng, 120 ng and 600 ng). ( a ) Antigen specific endpoint titres of (i) Nanopatch induced response by 6 ng, 60 ng or 120 ng of influenza antigen without QS-21, (ii) IM induced response by 10 μg of QS-21 with different dose of influenza antigen (6 ng, 60 ng, 120 ng and 600 ng, (iii) IM induced response by 50 μg of QS-21 with different dose of influenza antigen (6 ng, 60 ng, 120 ng and 600 ng; ( b ) Nanopatch and IM induce antigen specific IgG from different dose of QS-21 (1.5 μg, 10 μg and 50 μg) and ( c ) Haemagglutination Inhibition (HI) titres of one of the influenza strain A/Victoria/361/2011 (H3N2) from Nanopatch and IM at 60 ng and 120 ng of antigen with 1.5 μg, 10 μg and 50 μg of QS-21. ELISA antibody data represent the Mean ± SEM, statistical significance is when p

    Article Snippet: IgG1 and IgG2c isotypes responses were assessed using the same ELISA method (IgG1, ab97240; 1:4000, and IgG2c ab97255; 1:2000; Abcam).

    Techniques: Inhibition, Enzyme-linked Immunosorbent Assay

    HMGB1 released by HSV-2-infected cells activates fibroblast migration and stimulates HIV-1 expression. MRC5 fibroblasts were subjected to migration assays in the presence of (A) human recombinant HMGB1 or (B) supernatants from mock (M) or HSV-2-infected HEC-1 cells. HMGB1 concentrations reached 270 ng/ml in the supernatants collected from infected cells, versus 50 ng/ml in mock-infected cell media. Control experiments used glycyrrhizin or neutralizing antibodies against HMGB1 or its receptors RAGE, TLR-4 and TLR-2. Data are expressed as -fold increases in cell migration compared to non treated control cells. ACH-2 cells, that contain a latent HIV-1 provirus, were grown in the presence of (C) human recombinant HMGB1 or (D) supernatants from mock (M) or HSV-2-infected HEC-1 cells. P24 antigen was measured by ELISA after 36 h. Data are expressed as the -fold increase in p24 antigen compared with non treated control cells. Virion-free supernatants were obtained by ultracentrifugation. Results are either representative of several experiments (A, C, D) or expressed as the mean and SD of 3 independent experiments (B).

    Journal: PLoS ONE

    Article Title: Stepwise Release of Biologically Active HMGB1 during HSV-2 Infection

    doi: 10.1371/journal.pone.0016145

    Figure Lengend Snippet: HMGB1 released by HSV-2-infected cells activates fibroblast migration and stimulates HIV-1 expression. MRC5 fibroblasts were subjected to migration assays in the presence of (A) human recombinant HMGB1 or (B) supernatants from mock (M) or HSV-2-infected HEC-1 cells. HMGB1 concentrations reached 270 ng/ml in the supernatants collected from infected cells, versus 50 ng/ml in mock-infected cell media. Control experiments used glycyrrhizin or neutralizing antibodies against HMGB1 or its receptors RAGE, TLR-4 and TLR-2. Data are expressed as -fold increases in cell migration compared to non treated control cells. ACH-2 cells, that contain a latent HIV-1 provirus, were grown in the presence of (C) human recombinant HMGB1 or (D) supernatants from mock (M) or HSV-2-infected HEC-1 cells. P24 antigen was measured by ELISA after 36 h. Data are expressed as the -fold increase in p24 antigen compared with non treated control cells. Virion-free supernatants were obtained by ultracentrifugation. Results are either representative of several experiments (A, C, D) or expressed as the mean and SD of 3 independent experiments (B).

    Article Snippet: HIV production was evaluated by measuring p24 antigen with an ELISA method (Innogenetics, Les Ulis, France).

    Techniques: Infection, Migration, Expressing, Recombinant, Enzyme-linked Immunosorbent Assay

    Real-time PCR assessment of PPARγ gene expression in different human cell lines (A) and analysis of DNA-binding activity of PPARγ in nuclear extracts of human BSMCs by ELISA (B). C+, positive control; Sfm, serum-free medium; Rgz, rosiglitazone. Data are expressed as mean ± SEM. *** P

    Journal: British Journal of Pharmacology

    Article Title: Synergistic interaction between PPAR ligands and salbutamol on human bronchial smooth muscle cell proliferation

    doi: 10.1111/j.1476-5381.2012.02180.x

    Figure Lengend Snippet: Real-time PCR assessment of PPARγ gene expression in different human cell lines (A) and analysis of DNA-binding activity of PPARγ in nuclear extracts of human BSMCs by ELISA (B). C+, positive control; Sfm, serum-free medium; Rgz, rosiglitazone. Data are expressed as mean ± SEM. *** P

    Article Snippet: Nuclear protein extracts was obtained from serum-starved BSMC in the presence or absence of rosiglitazone 0.5 μM (Nuclear extract Kit, Cayman Chemical Company, Ann Arbor, MI, USA) and then the presence of functional PPARγ protein was confirmed by a sensitive and specific ELISA method (Cayman Chemical Company).

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Binding Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Positive Control

    Effects of MitoQ, MSU, TRX siRNA and TXNIP siRNA on TXNIP/NLRP3/IL-1β axis and pro-fibrotic protein expression and apoptosis in HK-2 cells cultured under HG conditions . A : IF showing FN ( Aa-c ) and Collagen I ( Ad-f ) expression in HK-2 cells subjected to HG treatment following MitoQ treatment. B: WB analysis of Caspase-1, cleaved IL-1β and IL-18 expression in HK-2 cells cultured under HG conditions and treated with different concentrations of MitoQ (50,100, 150 nM). C-H: ELISA of Caspase-1 ( C ), IL-1β ( D ), IL-18 ( E ), TGF-β ( F ) activity; TGF-β expression ( G ); and relative apoptosis ( H ) in HK-2 cells subjected to HG conditions and treated with different concentrations of MitoQ (50, 100, 150 nM). I: Luminex analysis of IL-1β, IL-18, FN and Collagen I expression in HK-2 cells subjected to an HG environment and treated with MitoQ, MSU or TXNIP siRNA. J, K: WB and densitometric analysis of NLRP3, IL-1β, IL-18 and FN expression in HK-2 cells exposed to an HG environment and pretreated with MitoQ, TRX siRNA or TXNIP siRNA. Values are the mean ± SE; *P

    Journal: Redox Biology

    Article Title: Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis

    doi: 10.1016/j.redox.2018.02.013

    Figure Lengend Snippet: Effects of MitoQ, MSU, TRX siRNA and TXNIP siRNA on TXNIP/NLRP3/IL-1β axis and pro-fibrotic protein expression and apoptosis in HK-2 cells cultured under HG conditions . A : IF showing FN ( Aa-c ) and Collagen I ( Ad-f ) expression in HK-2 cells subjected to HG treatment following MitoQ treatment. B: WB analysis of Caspase-1, cleaved IL-1β and IL-18 expression in HK-2 cells cultured under HG conditions and treated with different concentrations of MitoQ (50,100, 150 nM). C-H: ELISA of Caspase-1 ( C ), IL-1β ( D ), IL-18 ( E ), TGF-β ( F ) activity; TGF-β expression ( G ); and relative apoptosis ( H ) in HK-2 cells subjected to HG conditions and treated with different concentrations of MitoQ (50, 100, 150 nM). I: Luminex analysis of IL-1β, IL-18, FN and Collagen I expression in HK-2 cells subjected to an HG environment and treated with MitoQ, MSU or TXNIP siRNA. J, K: WB and densitometric analysis of NLRP3, IL-1β, IL-18 and FN expression in HK-2 cells exposed to an HG environment and pretreated with MitoQ, TRX siRNA or TXNIP siRNA. Values are the mean ± SE; *P

    Article Snippet: 2.13 Analysis of Caspase-1, IL-1β, IL-18 and TGF-β activity Caspase-1 (R & D Systems, USA), IL-1β, IL-18 and TGF-β (Ray Biotech, USA) expression and activity levels in the above cells were detected with the corresponding ELISA Kits, according to the manufacturers’ instructions , .

    Techniques: Expressing, Cell Culture, Western Blot, Enzyme-linked Immunosorbent Assay, Activity Assay, Luminex

    The effect of several antioxidants used as competitors in the monoclonal anti-MDA-LDL IgM ELISA. Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Cultivation and Immortalization of Human B-Cells Producing a Human Monoclonal IgM Antibody Binding to MDA-LDL: Further Evidence for Formation of Atherogenic MDA-LDL Adducts in Humans In Vivo

    doi: 10.1155/2017/6047142

    Figure Lengend Snippet: The effect of several antioxidants used as competitors in the monoclonal anti-MDA-LDL IgM ELISA. Aldehyde residues of MDA were reduced, presumably to alcohols, by antioxidants, thus precluding binding of the MCA.

    Article Snippet: Identification of Productive Clones At the end of the HAT selection period, supernatants of each well were tested for antibodies binding to Cu++ -oxidized LDL and/or MDA-LDL with commercially available ELISA methods (oLAb-ELISA, Biomedica, Vienna, Austria; MDA-LDL IgG/IgM, LDN Ltd., Nordhorn, Germany) adapted for the purposes of this investigation [ ].

    Techniques: Multiple Displacement Amplification, Enzyme-linked Immunosorbent Assay, Binding Assay