Journal: Micromachines

Article Title: Enhancement of Molecular Transport into Film Stacked Structures for Micro-Immunoassay by Unsteady Rotation.

doi: 10.3390/mi14040744

Figure Lengend Snippet: Figure 4. Scheme of sandwich ELISA using a conventional 96-well plate and 3D-Stack methods. Black Y is goat anti-human IgA antibody; Green Y with orrange dots is detection antibody with HRP; gray dots are BSA for blocking; red dot are human IgA, Orange squares in last step are AMB substrate.

Article Snippet: Human IgA ELISA Quantitation kit (E80-102), ELISA Blocking Buffer (E104), Wash Solution(E106), and TMB One Component Substrate (E102) were purchased from Bethyl Laboratories.

Techniques: Sandwich ELISA, Blocking Assay

Journal: Micromachines

Article Title: Enhancement of Molecular Transport into Film Stacked Structures for Micro-Immunoassay by Unsteady Rotation.

doi: 10.3390/mi14040744

Figure Lengend Snippet: Figure 15. Calibration curve for ELISA of IgA using 96 well and 96 well with 3D-Stack.

Article Snippet: Human IgA ELISA Quantitation kit (E80-102), ELISA Blocking Buffer (E104), Wash Solution(E106), and TMB One Component Substrate (E102) were purchased from Bethyl Laboratories.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Archives of Razi Institute

Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

doi: 10.22092/ARI.2021.356677.1890

Figure Lengend Snippet: Schematic representation of the principle of SARS-CoV-2-RBD neutralizing ELISA assay2.2. Assay Optimization

Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Archives of Razi Institute

Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

doi: 10.22092/ARI.2021.356677.1890

Figure Lengend Snippet: Standard curve of anti-SARS-COV-2-RBD nAbs for the in-house developed SARS-COV-2-RBD neutralizing ELISA assay

Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Archives of Razi Institute

Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

doi: 10.22092/ARI.2021.356677.1890

Figure Lengend Snippet: Mean ± standard deviation of OD values of different anti-SARS-COV-2-RBD neutralizing antibody standards generated by the designed neutralizing ELISA kit

Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

Techniques: Standard Deviation, Generated, Enzyme-linked Immunosorbent Assay, Concentration Assay, Negative Control, Positive Control

Journal: Archives of Razi Institute

Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

doi: 10.22092/ARI.2021.356677.1890

Figure Lengend Snippet: Cut-off value results of the in-house neutralizing ELISA; A) Optimized ROC analysis cut-off value of positive and B) Concentration and log concentration limits for the optimal established cut-off in this assay

Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Archives of Razi Institute

Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

doi: 10.22092/ARI.2021.356677.1890

Figure Lengend Snippet: Receiver operating characteristic curve analysis for the optimal cut-off value for detecting the positive anti-SARS-COV-2-RBD neutralizing antibodies using the in-house developed neutralizing ELISA kit

Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Archives of Razi Institute

Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

doi: 10.22092/ARI.2021.356677.1890

Figure Lengend Snippet: Serum panels using the in-house designed SARS-COV-2-RBD neutralizing ELISA assay; A) Positive control samples for the assay validation and B) Negative control samples for the assay validation

Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Biomarker Discovery, Negative Control, Concentration Assay

Journal: Archives of Razi Institute

Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

doi: 10.22092/ARI.2021.356677.1890

Figure Lengend Snippet: Agreement of the results between the in-house SARS-COV-2-RBD neutralizing ELISA assay and gold standard test at the in-house established cut-off

Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Archives of Razi Institute

Article Title: Application and Validation of SARS-CoV-2 RBD Neutralizing ELISA Assay

doi: 10.22092/ARI.2021.356677.1890

Figure Lengend Snippet: Validation parameters of the in-house SARS-COV-2-RBD neutralizing ELISA assay

Article Snippet: To immobilize the hACE2 protein at 400 ng/well in 100 μl of 1X working Coating Buffer on the microtiter plates wells, a calculated volume from Stock solution vial of Recombinant His-Tag-Human ACE2 protein in a concentration of 2.8 mg/ml (Raybiotech) was diluted immediately before usage with the prepared 1X working coating buffer from 5X stock ELISA Plate Coating Buffer (Elabscience) to a required volume at the appropriate concentration (4 ng/μl), which was determined in the initial experiments.

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay