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  • 99
    Thermo Fisher human interleukin 10 il 10 enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa kit
    Human Interleukin 10 Il 10 Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore rat tumor necrosis factor α tnf α enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa kit
    Rat Tumor Necrosis Factor α Tnf α Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hemagen enzyme linked immunosorbent assay enzyme linked immunosorbent assay
    Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay, supplied by Hemagen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sarstedt enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa
    Antibody responses in vaccinated mice against the GBSV components (n = 10-12). All data are presented as mean ± standard error of the mean. (A) Serum <t>ELISA</t> IgG antibodies against individual GBSV peptides. P values presented compared with mock-vaccinated animals. (B) Serum IgG isotypes against P6 and ScaAB after intranasal immunization with GBSV or LAIV + GBSV. ELISA indicates enzyme-linked <t>immunosorbent</t> assay; GBSV, group B streptococcus vaccine; LAIV, live attenuated influenza vaccine; PBS, phosphate-buffered saline.
    Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa, supplied by Sarstedt, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MyBiosource plasma wnt5a enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa kit
    Antibody responses in vaccinated mice against the GBSV components (n = 10-12). All data are presented as mean ± standard error of the mean. (A) Serum <t>ELISA</t> IgG antibodies against individual GBSV peptides. P values presented compared with mock-vaccinated animals. (B) Serum IgG isotypes against P6 and ScaAB after intranasal immunization with GBSV or LAIV + GBSV. ELISA indicates enzyme-linked <t>immunosorbent</t> assay; GBSV, group B streptococcus vaccine; LAIV, live attenuated influenza vaccine; PBS, phosphate-buffered saline.
    Plasma Wnt5a Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by MyBiosource, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Enzo Biochem competitive corticosterone cort enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa
    Plasma <t>corticosterone</t> (CORT) (A and D), urate glucocorticoid metabolites (GCM) (B and E), and feather CORT (C and F) levels measured by radioimmunoassay (RIA) are more reproducible than enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> across a range of sample dilutions . Symbols (open and filled squares, circles, triangles, and diamonds) represent a sample from an individual condor that was analyzed over a range of sample dilutions. CORT and GCM levels in diluted samples are expressed as a percent difference from expected values (horizontal dashed line at 0% diff.), based on levels measured in the most dilute sample (i.e., the lowest amount of sample in milligrams per 100 μL assay solution), and assumes sample matrix interferences are minimized in this most dilute sample. The vertical hash-marked region in each panel reflects the range of sample dilutions (x-axis) used for all samples in this study. The horizontal grey-shaded region reflects the CORT or GCM measurement uncertainty (± 2 RSD, based on intra-assay precision) for each assay and sample type; symbols within this region do not measurably differ from expected GC levels.
    Competitive Corticosterone Cort Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa
    Plasma <t>corticosterone</t> (CORT) (A and D), urate glucocorticoid metabolites (GCM) (B and E), and feather CORT (C and F) levels measured by radioimmunoassay (RIA) are more reproducible than enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> across a range of sample dilutions . Symbols (open and filled squares, circles, triangles, and diamonds) represent a sample from an individual condor that was analyzed over a range of sample dilutions. CORT and GCM levels in diluted samples are expressed as a percent difference from expected values (horizontal dashed line at 0% diff.), based on levels measured in the most dilute sample (i.e., the lowest amount of sample in milligrams per 100 μL assay solution), and assumes sample matrix interferences are minimized in this most dilute sample. The vertical hash-marked region in each panel reflects the range of sample dilutions (x-axis) used for all samples in this study. The horizontal grey-shaded region reflects the CORT or GCM measurement uncertainty (± 2 RSD, based on intra-assay precision) for each assay and sample type; symbols within this region do not measurably differ from expected GC levels.
    Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam immunosorbent assay enzyme linked immunosorbent assay
    Plasma <t>corticosterone</t> (CORT) (A and D), urate glucocorticoid metabolites (GCM) (B and E), and feather CORT (C and F) levels measured by radioimmunoassay (RIA) are more reproducible than enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> across a range of sample dilutions . Symbols (open and filled squares, circles, triangles, and diamonds) represent a sample from an individual condor that was analyzed over a range of sample dilutions. CORT and GCM levels in diluted samples are expressed as a percent difference from expected values (horizontal dashed line at 0% diff.), based on levels measured in the most dilute sample (i.e., the lowest amount of sample in milligrams per 100 μL assay solution), and assumes sample matrix interferences are minimized in this most dilute sample. The vertical hash-marked region in each panel reflects the range of sample dilutions (x-axis) used for all samples in this study. The horizontal grey-shaded region reflects the CORT or GCM measurement uncertainty (± 2 RSD, based on intra-assay precision) for each assay and sample type; symbols within this region do not measurably differ from expected GC levels.
    Immunosorbent Assay Enzyme Linked Immunosorbent Assay, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Lifespan Biosciences enzyme linked immunosorbent assay enzyme linked immunosorbent assay
    Plasma <t>corticosterone</t> (CORT) (A and D), urate glucocorticoid metabolites (GCM) (B and E), and feather CORT (C and F) levels measured by radioimmunoassay (RIA) are more reproducible than enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> across a range of sample dilutions . Symbols (open and filled squares, circles, triangles, and diamonds) represent a sample from an individual condor that was analyzed over a range of sample dilutions. CORT and GCM levels in diluted samples are expressed as a percent difference from expected values (horizontal dashed line at 0% diff.), based on levels measured in the most dilute sample (i.e., the lowest amount of sample in milligrams per 100 μL assay solution), and assumes sample matrix interferences are minimized in this most dilute sample. The vertical hash-marked region in each panel reflects the range of sample dilutions (x-axis) used for all samples in this study. The horizontal grey-shaded region reflects the CORT or GCM measurement uncertainty (± 2 RSD, based on intra-assay precision) for each assay and sample type; symbols within this region do not measurably differ from expected GC levels.
    Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay, supplied by Lifespan Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher enzyme linked immunosorbent elisa assay
    MnSOD2-induced reduction of T cell proliferation and IL-2 secretion PBMC from healthy donors were prepared by Ficoll density gradient centrifugation, labeled with CFSE according to the manufacturer 's instructions, and stimulated directly with anti-CD3 and anti-CD28 antibodies (mouse anti-human, BD, 1 μg/ml each) and IL-2 (100 U/ml). Cells were grown in the absence or presence of MnSOD2 (1U/ml) for 20 and 40 h prior to flow cytometry (A). IL-2 secretion was analyzed using commercial enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> (eBioscience, Austria) using “day 6” T cells at a density of 1 × 10 5 cells/well onto 96-well culture plates in the absence and presence of 1 U/ml MnSOD2 as described in Materials and Methods section (B). Data are represented as mean percentage (%) ± SD based on five independent experiments. * p -value
    Enzyme Linked Immunosorbent Elisa Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems interleukin 23 il 23 enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa kit
    MnSOD2-induced reduction of T cell proliferation and IL-2 secretion PBMC from healthy donors were prepared by Ficoll density gradient centrifugation, labeled with CFSE according to the manufacturer 's instructions, and stimulated directly with anti-CD3 and anti-CD28 antibodies (mouse anti-human, BD, 1 μg/ml each) and IL-2 (100 U/ml). Cells were grown in the absence or presence of MnSOD2 (1U/ml) for 20 and 40 h prior to flow cytometry (A). IL-2 secretion was analyzed using commercial enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> (eBioscience, Austria) using “day 6” T cells at a density of 1 × 10 5 cells/well onto 96-well culture plates in the absence and presence of 1 U/ml MnSOD2 as described in Materials and Methods section (B). Data are represented as mean percentage (%) ± SD based on five independent experiments. * p -value
    Interleukin 23 Il 23 Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa kit
    MnSOD2-induced reduction of T cell proliferation and IL-2 secretion PBMC from healthy donors were prepared by Ficoll density gradient centrifugation, labeled with CFSE according to the manufacturer 's instructions, and stimulated directly with anti-CD3 and anti-CD28 antibodies (mouse anti-human, BD, 1 μg/ml each) and IL-2 (100 U/ml). Cells were grown in the absence or presence of MnSOD2 (1U/ml) for 20 and 40 h prior to flow cytometry (A). IL-2 secretion was analyzed using commercial enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> (eBioscience, Austria) using “day 6” T cells at a density of 1 × 10 5 cells/well onto 96-well culture plates in the absence and presence of 1 U/ml MnSOD2 as described in Materials and Methods section (B). Data are represented as mean percentage (%) ± SD based on five independent experiments. * p -value
    Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa kits
    MnSOD2-induced reduction of T cell proliferation and IL-2 secretion PBMC from healthy donors were prepared by Ficoll density gradient centrifugation, labeled with CFSE according to the manufacturer 's instructions, and stimulated directly with anti-CD3 and anti-CD28 antibodies (mouse anti-human, BD, 1 μg/ml each) and IL-2 (100 U/ml). Cells were grown in the absence or presence of MnSOD2 (1U/ml) for 20 and 40 h prior to flow cytometry (A). IL-2 secretion was analyzed using commercial enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> (eBioscience, Austria) using “day 6” T cells at a density of 1 × 10 5 cells/well onto 96-well culture plates in the absence and presence of 1 U/ml MnSOD2 as described in Materials and Methods section (B). Data are represented as mean percentage (%) ± SD based on five independent experiments. * p -value
    Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa Kits, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore enzyme linked immunosorbent assay enzyme linked immunosorbent assay plates
    FAT1 domain/epitope specificity of mAb198.3. ( A ) Scheme of FAT1 constructs generated to identify the mAb198.3 epitope. Below the scheme are reported the WB of HeLa and HEK-293T cells transfected with the FAT1 constructs or the D12 domain expressed in E. coli . ( B ) Enzyme-linked <t>immunosorbent</t> assay peptide competition analysis: ELISA plates coated with rD8 and rD12 were coincubated with mAb198.3 and different concentrations of the 25 mer peptides 1 and 7 or an irrelevant peptide. The binding inhibition was measured as compared with samples incubated with mAb198.3 alone. ( C and D ) Fluorescence-activated cell sorting and IHC peptide competition analysis on HCT15 cells or colon cancer tissues, respectively, incubated with mAb198.3 and the 25 mer peptides 1 or 7 and the irrelevant peptide. ( E ) Sequence alignment of the FAT1 domains D8 and D12 containing the mAb198.3 epitope. The consensus is reported above the alignment. The peptides 7 and 1 are highlighted.
    Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EUROIMMUN galactomannoprotein gp enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa
    FAT1 domain/epitope specificity of mAb198.3. ( A ) Scheme of FAT1 constructs generated to identify the mAb198.3 epitope. Below the scheme are reported the WB of HeLa and HEK-293T cells transfected with the FAT1 constructs or the D12 domain expressed in E. coli . ( B ) Enzyme-linked <t>immunosorbent</t> assay peptide competition analysis: ELISA plates coated with rD8 and rD12 were coincubated with mAb198.3 and different concentrations of the 25 mer peptides 1 and 7 or an irrelevant peptide. The binding inhibition was measured as compared with samples incubated with mAb198.3 alone. ( C and D ) Fluorescence-activated cell sorting and IHC peptide competition analysis on HCT15 cells or colon cancer tissues, respectively, incubated with mAb198.3 and the 25 mer peptides 1 or 7 and the irrelevant peptide. ( E ) Sequence alignment of the FAT1 domains D8 and D12 containing the mAb198.3 epitope. The consensus is reported above the alignment. The peptides 7 and 1 are highlighted.
    Galactomannoprotein Gp Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche bromodeoxyuridine brdu enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa
    FAT1 domain/epitope specificity of mAb198.3. ( A ) Scheme of FAT1 constructs generated to identify the mAb198.3 epitope. Below the scheme are reported the WB of HeLa and HEK-293T cells transfected with the FAT1 constructs or the D12 domain expressed in E. coli . ( B ) Enzyme-linked <t>immunosorbent</t> assay peptide competition analysis: ELISA plates coated with rD8 and rD12 were coincubated with mAb198.3 and different concentrations of the 25 mer peptides 1 and 7 or an irrelevant peptide. The binding inhibition was measured as compared with samples incubated with mAb198.3 alone. ( C and D ) Fluorescence-activated cell sorting and IHC peptide competition analysis on HCT15 cells or colon cancer tissues, respectively, incubated with mAb198.3 and the 25 mer peptides 1 or 7 and the irrelevant peptide. ( E ) Sequence alignment of the FAT1 domains D8 and D12 containing the mAb198.3 epitope. The consensus is reported above the alignment. The peptides 7 and 1 are highlighted.
    Bromodeoxyuridine Brdu Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore fgf23 ifgf23 enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa kit
    FAT1 domain/epitope specificity of mAb198.3. ( A ) Scheme of FAT1 constructs generated to identify the mAb198.3 epitope. Below the scheme are reported the WB of HeLa and HEK-293T cells transfected with the FAT1 constructs or the D12 domain expressed in E. coli . ( B ) Enzyme-linked <t>immunosorbent</t> assay peptide competition analysis: ELISA plates coated with rD8 and rD12 were coincubated with mAb198.3 and different concentrations of the 25 mer peptides 1 and 7 or an irrelevant peptide. The binding inhibition was measured as compared with samples incubated with mAb198.3 alone. ( C and D ) Fluorescence-activated cell sorting and IHC peptide competition analysis on HCT15 cells or colon cancer tissues, respectively, incubated with mAb198.3 and the 25 mer peptides 1 or 7 and the irrelevant peptide. ( E ) Sequence alignment of the FAT1 domains D8 and D12 containing the mAb198.3 epitope. The consensus is reported above the alignment. The peptides 7 and 1 are highlighted.
    Fgf23 Ifgf23 Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems solid phase enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa
    FAT1 domain/epitope specificity of mAb198.3. ( A ) Scheme of FAT1 constructs generated to identify the mAb198.3 epitope. Below the scheme are reported the WB of HeLa and HEK-293T cells transfected with the FAT1 constructs or the D12 domain expressed in E. coli . ( B ) Enzyme-linked <t>immunosorbent</t> assay peptide competition analysis: ELISA plates coated with rD8 and rD12 were coincubated with mAb198.3 and different concentrations of the 25 mer peptides 1 and 7 or an irrelevant peptide. The binding inhibition was measured as compared with samples incubated with mAb198.3 alone. ( C and D ) Fluorescence-activated cell sorting and IHC peptide competition analysis on HCT15 cells or colon cancer tissues, respectively, incubated with mAb198.3 and the 25 mer peptides 1 or 7 and the irrelevant peptide. ( E ) Sequence alignment of the FAT1 domains D8 and D12 containing the mAb198.3 epitope. The consensus is reported above the alignment. The peptides 7 and 1 are highlighted.
    Solid Phase Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fak total enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa kits
    FAT1 domain/epitope specificity of mAb198.3. ( A ) Scheme of FAT1 constructs generated to identify the mAb198.3 epitope. Below the scheme are reported the WB of HeLa and HEK-293T cells transfected with the FAT1 constructs or the D12 domain expressed in E. coli . ( B ) Enzyme-linked <t>immunosorbent</t> assay peptide competition analysis: ELISA plates coated with rD8 and rD12 were coincubated with mAb198.3 and different concentrations of the 25 mer peptides 1 and 7 or an irrelevant peptide. The binding inhibition was measured as compared with samples incubated with mAb198.3 alone. ( C and D ) Fluorescence-activated cell sorting and IHC peptide competition analysis on HCT15 cells or colon cancer tissues, respectively, incubated with mAb198.3 and the 25 mer peptides 1 or 7 and the irrelevant peptide. ( E ) Sequence alignment of the FAT1 domains D8 and D12 containing the mAb198.3 epitope. The consensus is reported above the alignment. The peptides 7 and 1 are highlighted.
    Fak Total Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems sensitivity hs enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa
    Plasma myeloperoxidase (MPO) and histone levels. MPO ( A ), histone ( B ) and histone H3 ( C ) levels were measured by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma from non-pregnant (Non-P), normal pregnant (NP) and pre-eclampsia (PE) women. Sample numbers ( n ) in each group are indicated. Data were analysed by Kruskal–Wallis and Mann–Whitney tests with Bonferroni correction for multiple comparisons. ns, not statistically significant. Correlations between plasma deoxyribonucleic acid (DNA) levels and MPO ( D ) or histone ( E ) levels in normal pregnant and PE women were analysed by computing Pearson’s correlation coefficient.
    Sensitivity Hs Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche transcriptase rt enzyme linked immunosorbent assay enzyme linked immunosorbent assay elisa
    Plasma myeloperoxidase (MPO) and histone levels. MPO ( A ), histone ( B ) and histone H3 ( C ) levels were measured by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma from non-pregnant (Non-P), normal pregnant (NP) and pre-eclampsia (PE) women. Sample numbers ( n ) in each group are indicated. Data were analysed by Kruskal–Wallis and Mann–Whitney tests with Bonferroni correction for multiple comparisons. ns, not statistically significant. Correlations between plasma deoxyribonucleic acid (DNA) levels and MPO ( D ) or histone ( E ) levels in normal pregnant and PE women were analysed by computing Pearson’s correlation coefficient.
    Transcriptase Rt Enzyme Linked Immunosorbent Assay Enzyme Linked Immunosorbent Assay Elisa, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Plasma myeloperoxidase (MPO) and histone levels. MPO ( A ), histone ( B ) and histone H3 ( C ) levels were measured by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma from non-pregnant (Non-P), normal pregnant (NP) and pre-eclampsia (PE) women. Sample numbers ( n ) in each group are indicated. Data were analysed by Kruskal–Wallis and Mann–Whitney tests with Bonferroni correction for multiple comparisons. ns, not statistically significant. Correlations between plasma deoxyribonucleic acid (DNA) levels and MPO ( D ) or histone ( E ) levels in normal pregnant and PE women were analysed by computing Pearson’s correlation coefficient.
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    Antibody responses in vaccinated mice against the GBSV components (n = 10-12). All data are presented as mean ± standard error of the mean. (A) Serum ELISA IgG antibodies against individual GBSV peptides. P values presented compared with mock-vaccinated animals. (B) Serum IgG isotypes against P6 and ScaAB after intranasal immunization with GBSV or LAIV + GBSV. ELISA indicates enzyme-linked immunosorbent assay; GBSV, group B streptococcus vaccine; LAIV, live attenuated influenza vaccine; PBS, phosphate-buffered saline.

    Journal: Virology : Research and Treatment

    Article Title: Prevention of Influenza A(H7N9) and Bacterial Infections in Mice Using Intranasal Immunization With Live Influenza Vaccine and the Group B Streptococcus Recombinant Polypeptides

    doi: 10.1177/1178122X17710949

    Figure Lengend Snippet: Antibody responses in vaccinated mice against the GBSV components (n = 10-12). All data are presented as mean ± standard error of the mean. (A) Serum ELISA IgG antibodies against individual GBSV peptides. P values presented compared with mock-vaccinated animals. (B) Serum IgG isotypes against P6 and ScaAB after intranasal immunization with GBSV or LAIV + GBSV. ELISA indicates enzyme-linked immunosorbent assay; GBSV, group B streptococcus vaccine; LAIV, live attenuated influenza vaccine; PBS, phosphate-buffered saline.

    Article Snippet: The enzyme-linked immunosorbent assay Enzyme-linked immunosorbent assay (ELISA) was conducted to determine serum IgG, IgG1, IgG2a, and nasal IgA antibodies in 96-well microplates (Sarstedt AG & Co, Nümbrecht, Germany) as previously described.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Plasma corticosterone (CORT) (A and D), urate glucocorticoid metabolites (GCM) (B and E), and feather CORT (C and F) levels measured by radioimmunoassay (RIA) are more reproducible than enzyme-linked immunosorbent assay (ELISA) across a range of sample dilutions . Symbols (open and filled squares, circles, triangles, and diamonds) represent a sample from an individual condor that was analyzed over a range of sample dilutions. CORT and GCM levels in diluted samples are expressed as a percent difference from expected values (horizontal dashed line at 0% diff.), based on levels measured in the most dilute sample (i.e., the lowest amount of sample in milligrams per 100 μL assay solution), and assumes sample matrix interferences are minimized in this most dilute sample. The vertical hash-marked region in each panel reflects the range of sample dilutions (x-axis) used for all samples in this study. The horizontal grey-shaded region reflects the CORT or GCM measurement uncertainty (± 2 RSD, based on intra-assay precision) for each assay and sample type; symbols within this region do not measurably differ from expected GC levels.

    Journal: PLoS ONE

    Article Title: Glucocorticoid measurement in plasma, urates, and feathers from California condors (Gymnogyps californianus) in response to a human-induced stressor

    doi: 10.1371/journal.pone.0205565

    Figure Lengend Snippet: Plasma corticosterone (CORT) (A and D), urate glucocorticoid metabolites (GCM) (B and E), and feather CORT (C and F) levels measured by radioimmunoassay (RIA) are more reproducible than enzyme-linked immunosorbent assay (ELISA) across a range of sample dilutions . Symbols (open and filled squares, circles, triangles, and diamonds) represent a sample from an individual condor that was analyzed over a range of sample dilutions. CORT and GCM levels in diluted samples are expressed as a percent difference from expected values (horizontal dashed line at 0% diff.), based on levels measured in the most dilute sample (i.e., the lowest amount of sample in milligrams per 100 μL assay solution), and assumes sample matrix interferences are minimized in this most dilute sample. The vertical hash-marked region in each panel reflects the range of sample dilutions (x-axis) used for all samples in this study. The horizontal grey-shaded region reflects the CORT or GCM measurement uncertainty (± 2 RSD, based on intra-assay precision) for each assay and sample type; symbols within this region do not measurably differ from expected GC levels.

    Article Snippet: We evaluated the precision and accuracy of a competitive corticosterone (CORT) enzyme-linked immunosorbent assay (ELISA) (Cat. No. ADI-900-097, Enzo Life Sciences), and a corticosterone 125 I double antibody radioimmunoassay (RIA) kit (Prod.

    Techniques: RIA Assay, Enzyme-linked Immunosorbent Assay, Intra Assay

    Measurements by radioimmunoassay (RIA) vs. enzyme-linked immunosorbent assay (ELISA) are different but significantly correlated for plasma corticosterone (CORT) and urate glucocorticoid metabolites (GCM). Each data point represents a condor sample measured by both RIA and ELISA. The dashed line indicates idealized agreement (y = x) between the RIA and ELISA values. In all sample types ELISA measurements trended lower than RIA measurements. (A) Plasma CORT concentrations by ELISA and RIA. (B) Urate GCM concentrations measured by RIA and ELISA, levels measured by ELISA are systematically lower by ~50%-600% compared to RIA. (C) Feather CORT concentrations measured by RIA and ELISA appear to agree only for lower CORT concentration samples (

    Journal: PLoS ONE

    Article Title: Glucocorticoid measurement in plasma, urates, and feathers from California condors (Gymnogyps californianus) in response to a human-induced stressor

    doi: 10.1371/journal.pone.0205565

    Figure Lengend Snippet: Measurements by radioimmunoassay (RIA) vs. enzyme-linked immunosorbent assay (ELISA) are different but significantly correlated for plasma corticosterone (CORT) and urate glucocorticoid metabolites (GCM). Each data point represents a condor sample measured by both RIA and ELISA. The dashed line indicates idealized agreement (y = x) between the RIA and ELISA values. In all sample types ELISA measurements trended lower than RIA measurements. (A) Plasma CORT concentrations by ELISA and RIA. (B) Urate GCM concentrations measured by RIA and ELISA, levels measured by ELISA are systematically lower by ~50%-600% compared to RIA. (C) Feather CORT concentrations measured by RIA and ELISA appear to agree only for lower CORT concentration samples (

    Article Snippet: We evaluated the precision and accuracy of a competitive corticosterone (CORT) enzyme-linked immunosorbent assay (ELISA) (Cat. No. ADI-900-097, Enzo Life Sciences), and a corticosterone 125 I double antibody radioimmunoassay (RIA) kit (Prod.

    Techniques: RIA Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay

    MnSOD2-induced reduction of T cell proliferation and IL-2 secretion PBMC from healthy donors were prepared by Ficoll density gradient centrifugation, labeled with CFSE according to the manufacturer 's instructions, and stimulated directly with anti-CD3 and anti-CD28 antibodies (mouse anti-human, BD, 1 μg/ml each) and IL-2 (100 U/ml). Cells were grown in the absence or presence of MnSOD2 (1U/ml) for 20 and 40 h prior to flow cytometry (A). IL-2 secretion was analyzed using commercial enzyme-linked immunosorbent assay (ELISA) (eBioscience, Austria) using “day 6” T cells at a density of 1 × 10 5 cells/well onto 96-well culture plates in the absence and presence of 1 U/ml MnSOD2 as described in Materials and Methods section (B). Data are represented as mean percentage (%) ± SD based on five independent experiments. * p -value

    Journal: Oncotarget

    Article Title: VHL-dependent alterations in the secretome of renal cell carcinoma: Association with immune cell response?

    doi:

    Figure Lengend Snippet: MnSOD2-induced reduction of T cell proliferation and IL-2 secretion PBMC from healthy donors were prepared by Ficoll density gradient centrifugation, labeled with CFSE according to the manufacturer 's instructions, and stimulated directly with anti-CD3 and anti-CD28 antibodies (mouse anti-human, BD, 1 μg/ml each) and IL-2 (100 U/ml). Cells were grown in the absence or presence of MnSOD2 (1U/ml) for 20 and 40 h prior to flow cytometry (A). IL-2 secretion was analyzed using commercial enzyme-linked immunosorbent assay (ELISA) (eBioscience, Austria) using “day 6” T cells at a density of 1 × 10 5 cells/well onto 96-well culture plates in the absence and presence of 1 U/ml MnSOD2 as described in Materials and Methods section (B). Data are represented as mean percentage (%) ± SD based on five independent experiments. * p -value

    Article Snippet: Determination cell proliferation and IL-2 secretion in the absence and presence of MnSOD2 IL-2 secretion was analyzed using commercial enzyme-linked immunosorbent (ELISA) assay (eBioscience, Austria) based on the supplier's protocol.

    Techniques: Gradient Centrifugation, Labeling, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay

    Interleukin (IL)-12 production by human peripheral blood mononuclear cells (PBMC) co-cultured with Lactobacillus casei ssp. casei . The cells were co-cultured with the indicated doses of live L. casei ssp. casei, L. paracasei ssp. paracasei or L. acidophilus for 48 h. IL-12 production was analysed by enzyme-linked immunosorbent assay (ELISA). Data are shown as the mean ± s.e.m. of three independent experiments.

    Journal: Clinical and Experimental Immunology

    Article Title: Natural killer cell activities of synbiotic Lactobacillus casei ssp. casei in conjunction with dextran

    doi: 10.1111/j.1365-2249.2005.02975.x

    Figure Lengend Snippet: Interleukin (IL)-12 production by human peripheral blood mononuclear cells (PBMC) co-cultured with Lactobacillus casei ssp. casei . The cells were co-cultured with the indicated doses of live L. casei ssp. casei, L. paracasei ssp. paracasei or L. acidophilus for 48 h. IL-12 production was analysed by enzyme-linked immunosorbent assay (ELISA). Data are shown as the mean ± s.e.m. of three independent experiments.

    Article Snippet: Culture supernatants were collected and analysed by enzyme-linked immunosorbent assay (ELISA) for secreted IL-12 ( e Bioscience, San Diego, CA, USA).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

    FAT1 domain/epitope specificity of mAb198.3. ( A ) Scheme of FAT1 constructs generated to identify the mAb198.3 epitope. Below the scheme are reported the WB of HeLa and HEK-293T cells transfected with the FAT1 constructs or the D12 domain expressed in E. coli . ( B ) Enzyme-linked immunosorbent assay peptide competition analysis: ELISA plates coated with rD8 and rD12 were coincubated with mAb198.3 and different concentrations of the 25 mer peptides 1 and 7 or an irrelevant peptide. The binding inhibition was measured as compared with samples incubated with mAb198.3 alone. ( C and D ) Fluorescence-activated cell sorting and IHC peptide competition analysis on HCT15 cells or colon cancer tissues, respectively, incubated with mAb198.3 and the 25 mer peptides 1 or 7 and the irrelevant peptide. ( E ) Sequence alignment of the FAT1 domains D8 and D12 containing the mAb198.3 epitope. The consensus is reported above the alignment. The peptides 7 and 1 are highlighted.

    Journal: British Journal of Cancer

    Article Title: FAT1: a potential target for monoclonal antibody therapy in colon cancer

    doi: 10.1038/bjc.2016.145

    Figure Lengend Snippet: FAT1 domain/epitope specificity of mAb198.3. ( A ) Scheme of FAT1 constructs generated to identify the mAb198.3 epitope. Below the scheme are reported the WB of HeLa and HEK-293T cells transfected with the FAT1 constructs or the D12 domain expressed in E. coli . ( B ) Enzyme-linked immunosorbent assay peptide competition analysis: ELISA plates coated with rD8 and rD12 were coincubated with mAb198.3 and different concentrations of the 25 mer peptides 1 and 7 or an irrelevant peptide. The binding inhibition was measured as compared with samples incubated with mAb198.3 alone. ( C and D ) Fluorescence-activated cell sorting and IHC peptide competition analysis on HCT15 cells or colon cancer tissues, respectively, incubated with mAb198.3 and the 25 mer peptides 1 or 7 and the irrelevant peptide. ( E ) Sequence alignment of the FAT1 domains D8 and D12 containing the mAb198.3 epitope. The consensus is reported above the alignment. The peptides 7 and 1 are highlighted.

    Article Snippet: Enzyme-linked immunosorbent assay Enzyme-linked immunosorbent assay plates (NuncMaxisorp; Sigma Aldrich, St Louis, MO, USA) were coated with 1 μ g of His-tagged FAT1 recombinant domains in PBS (pH 7.4) at 4 °C overnight.

    Techniques: Construct, Generated, Western Blot, Transfection, Enzyme-linked Immunosorbent Assay, Binding Assay, Inhibition, Incubation, Fluorescence, FACS, Immunohistochemistry, Sequencing

    Plasma myeloperoxidase (MPO) and histone levels. MPO ( A ), histone ( B ) and histone H3 ( C ) levels were measured by enzyme-linked immunosorbent assay (ELISA) in plasma from non-pregnant (Non-P), normal pregnant (NP) and pre-eclampsia (PE) women. Sample numbers ( n ) in each group are indicated. Data were analysed by Kruskal–Wallis and Mann–Whitney tests with Bonferroni correction for multiple comparisons. ns, not statistically significant. Correlations between plasma deoxyribonucleic acid (DNA) levels and MPO ( D ) or histone ( E ) levels in normal pregnant and PE women were analysed by computing Pearson’s correlation coefficient.

    Journal: Thrombosis and haemostasis

    Article Title: Increased Neutrophil Activation and Plasma DNA Levels in Patients with Pre-Eclampsia

    doi: 10.1055/s-0038-1675788

    Figure Lengend Snippet: Plasma myeloperoxidase (MPO) and histone levels. MPO ( A ), histone ( B ) and histone H3 ( C ) levels were measured by enzyme-linked immunosorbent assay (ELISA) in plasma from non-pregnant (Non-P), normal pregnant (NP) and pre-eclampsia (PE) women. Sample numbers ( n ) in each group are indicated. Data were analysed by Kruskal–Wallis and Mann–Whitney tests with Bonferroni correction for multiple comparisons. ns, not statistically significant. Correlations between plasma deoxyribonucleic acid (DNA) levels and MPO ( D ) or histone ( E ) levels in normal pregnant and PE women were analysed by computing Pearson’s correlation coefficient.

    Article Snippet: Plasma MPO levels were measured by enzyme-linked immunosorbent assay (ELISA) (R & D Systems, DMYE00B).

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Effect of Escherichia coli –secretory immunoglobulin A (SIgA) immune complexes on mucus secretion. Three‐dimensional (3D) model constructs were collected after stimulation with HS or SIgA or E. coli strain HS (HS) and SIgA. Cultures with media (none) only or with immunoglobulin (Ig)G were used as controls. (a) Immunochemistry staining for mucin‐2 (MUC2) levels. Human small intestine (control) (left panel) and 3D model are shown at higher magnification (×100). (b) After 3 (open bars) and 6 h (closed bars) of stimulation, transcriptional analysis of MUC2 regulation was performed by quantitative reverse transcription–polymerase chain reaction (qRT–PCR). Values represent means ± standard error (s.e.) of three independent experiments with two replicates each. (c) After 6 h of stimulation, MUC2 protein expression was also detected by Western blot. (d) Values represent means ± s.e. of four independent Western blot experiments. Band density was normalized to glyceraldehyde 3‐phosphate dehydrogenase (GAPDH). (e) After 3 (open bars) and 6 h (closed bars) of stimulation, secretion of MUC2 into the supernatants was measured by enzyme‐linked immunosorbent assay (ELISA). The dashed lines represent the MUC2 levels of the cells exposed to media only (none). * P‐ values

    Journal: Clinical and Experimental Immunology

    Article Title: Free and complexed‐secretory immunoglobulin A triggers distinct intestinal epithelial cell responses

    doi: 10.1111/cei.12801

    Figure Lengend Snippet: Effect of Escherichia coli –secretory immunoglobulin A (SIgA) immune complexes on mucus secretion. Three‐dimensional (3D) model constructs were collected after stimulation with HS or SIgA or E. coli strain HS (HS) and SIgA. Cultures with media (none) only or with immunoglobulin (Ig)G were used as controls. (a) Immunochemistry staining for mucin‐2 (MUC2) levels. Human small intestine (control) (left panel) and 3D model are shown at higher magnification (×100). (b) After 3 (open bars) and 6 h (closed bars) of stimulation, transcriptional analysis of MUC2 regulation was performed by quantitative reverse transcription–polymerase chain reaction (qRT–PCR). Values represent means ± standard error (s.e.) of three independent experiments with two replicates each. (c) After 6 h of stimulation, MUC2 protein expression was also detected by Western blot. (d) Values represent means ± s.e. of four independent Western blot experiments. Band density was normalized to glyceraldehyde 3‐phosphate dehydrogenase (GAPDH). (e) After 3 (open bars) and 6 h (closed bars) of stimulation, secretion of MUC2 into the supernatants was measured by enzyme‐linked immunosorbent assay (ELISA). The dashed lines represent the MUC2 levels of the cells exposed to media only (none). * P‐ values

    Article Snippet: Mucus and cytokine production Levels of mucus in culture supernatants were measured by using a commercial mucin 2 (MUC2) enzyme‐linked immunosorbent assay (ELISA) (MyBioSource, San Diego, CA, USA).

    Techniques: Construct, Staining, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay