Journal: Retrovirology

Article Title: Interference of retroviral envelope with vaccine-induced CD8 + T cell responses is relieved by co-administration of cytokine-encoding vectors

doi: 10.1186/s12977-017-0352-7

Figure Lengend Snippet: Induction of GagL 85–93 -specific CD8 + T cells by co-immunization with cytokine-encoding plasmids. CB6F1 mice were immunized once with a DNA plasmid encoding Leader-Gag, a mix of plasmids encoding Leader-Gag and Env (LG + Env), or a mix of plasmids encoding Leader-Gag, Env and cytokines as indicated; 25 µg of each plasmid were injected intramuscularly followed by in vivo electroporation. Two weeks after immunization, the frequency of GagL 85–93 -specific CD8 + T cells in blood cells was analysed by MHC I tetramer staining ( a ), the production of IFNγ by GagL 85–93 -specific CD8 + T cells was analysed by intracellular cytokine staining after peptide restimulation ( b ). Data were acquired in two to four independent experiments with three to four mice per group per experiment. Each dot indicates an individual mouse, the bars indicate the mean values. Data were analysed by Kruskall–Wallis One Way Analysis of Variance on Ranks and Dunn’s multiple comparison procedure, statistically significant differences ( P < 0.05) compared to unvaccinated mice are indicated by *, significant differences compared to mice immunized with the combination of Leader-Gag and Env plasmids (LG + Env) are indicated by #

Article Snippet: Immediately following the DNA injection, in vivo electroporation was performed using the BTX AgilePulse system (BTX Molecular Delivery Systems, Holliston, MA) or the Ichor electroporation system (Ichor Medical Systems, San Diego, CA) using electroporation protocols described before [ , ].

Techniques: Plasmid Preparation, Injection, In Vivo, Electroporation, Staining, Comparison

Journal: Retrovirology

Article Title: Interference of retroviral envelope with vaccine-induced CD8 + T cell responses is relieved by co-administration of cytokine-encoding vectors

doi: 10.1186/s12977-017-0352-7

Figure Lengend Snippet: Binding antibody responses to plasmid combination vaccines. CB6F1 mice were immunized once with a DNA plasmid encoding Leader-Gag, a mix of plasmids encoding Leader-Gag and Env (LG + Env), or a mix of plasmids encoding Leader-Gag, Env and cytokines as indicated; 25 µg of each plasmid were injected intramuscularly followed by in vivo electroporation. Two weeks after immunization, blood samples were collected and F-MuLV-binding antibodies were analysed. The figure shows absorption values for plasma samples diluted 1:50 in PBS. Data were acquired in two to four independent experiments with three to four mice per group per experiment. Each dot indicates an individual mouse, bars indicate median values. Data were analysed by Kruskall–Wallis One Way Analysis of Variance on Ranks and Dunn’s multiple comparison procedure for statistical significance; statistically significant differences ( P < 0.05) compared to unvaccinated mice are indicated by *, significant differences compared to mice immunized with the combination of Leader-Gag and Env plasmids (LG + Env) are indicated by #

Article Snippet: Immediately following the DNA injection, in vivo electroporation was performed using the BTX AgilePulse system (BTX Molecular Delivery Systems, Holliston, MA) or the Ichor electroporation system (Ichor Medical Systems, San Diego, CA) using electroporation protocols described before [ , ].

Techniques: Binding Assay, Plasmid Preparation, Vaccines, Injection, In Vivo, Electroporation, Clinical Proteomics, Comparison

Journal: Retrovirology

Article Title: Interference of retroviral envelope with vaccine-induced CD8 + T cell responses is relieved by co-administration of cytokine-encoding vectors

doi: 10.1186/s12977-017-0352-7

Figure Lengend Snippet: Protection from FV challenge infection by co-immunization with cytokine-encoding plasmids. CB6F1 mice were immunized once with a DNA plasmid encoding Leader-Gag, a mix of plasmids encoding Leader-Gag and Env (LG + Env), or a mix of plasmids encoding Leader-Gag, Env and cytokines as indicated; 25 µg of each plasmid were injected intramuscularly followed by in vivo electroporation. Three weeks after immunization, mice were infected with 5000 spleen focus forming units FV. The development of splenomegaly was monitored by palpation of the spleen twice a week ( a ). Three weeks after FV challenge, mice were sacrificed and spleen weight ( b ) and viral loads in spleen cells were determined ( c ). Data were acquired in two to four independent experiments with three to four mice per group per experiment. The bars in ( a ) indicate the mean values, whiskers indicate the standard error of the means. Each dot ( b , c ) indicates an individual mouse, bars indicate mean ( b ) or median ( c ) values. Data were analysed by Kruskall–Wallis One Way Analysis of Variance on Ranks and Dunn’s multiple comparison procedure, statistically significant differences ( P < 0.05) compared to unvaccinated mice are indicated by *, significant differences compared to mice immunized with the combination of Leader-Gag and Env plasmids (LG + Env) are indicated by #

Article Snippet: Immediately following the DNA injection, in vivo electroporation was performed using the BTX AgilePulse system (BTX Molecular Delivery Systems, Holliston, MA) or the Ichor electroporation system (Ichor Medical Systems, San Diego, CA) using electroporation protocols described before [ , ].

Techniques: Infection, Plasmid Preparation, Injection, In Vivo, Electroporation, Comparison

Journal:

Article Title: Challenges and opportunities in dermal/transdermal delivery

doi:

Figure Lengend Snippet: Ongoing clinical trials involving microporation techniques for transdermal delivery.

Article Snippet: Uppsala University (Karolinska Institutet and Cyto Pulse Sciences, Inc.) , DNA vaccine delivered by intradermal electroporation (device: Derma Vax) , Prostate cancer.

Techniques: Clinical Proteomics, Electroporation, Control, Injection