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  • 96
    JEOL transmission electron microscope
    Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 96/100, based on 21623 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    JEOL scanning electron microscope
    <t>Field-emission</t> <t>scanning</t> <t>electron</t> <t>microscope</t> morphologies of surfaces and cross sections of NBT thick films annealed at 750°C with various thicknesses.
    Scanning Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 95/100, based on 10045 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/scanning electron microscope/product/JEOL
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    95
    Hitachi Ltd scanning electron microscope
    <t>Field</t> <t>emission</t> <t>scanning</t> <t>electron</t> micrographs of the fibres. The surface structure of the silk fibres derived from the non-transgenic silkworm lineages ( a ) and the transgenic silkworm lineages MASP1-2-1 ( b ), MASP1-12-5 ( c ) and MASP1-16-2 ( d ); the cross section structure of the silk fibres derived from the non-transgenic silkworm lineages ( a’ , a” ) and the transgenic silkworm lineages MASP1-2-1 (b’ , b” ), MASP1-12-5 ( c’ , c” ) and MASP1-16-2 ( d’ , d” ). Scale bars in a-d, 10μm; in a’-d’, 5 μm; in a”-d”, 1 μm.
    Scanning Electron Microscope, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 95/100, based on 8594 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/scanning electron microscope/product/Hitachi Ltd
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    95
    JEOL scanning electron microscopy
    TEM image (left) and FE-SEM image (right) with 1% uranyl acetate negative staining. ( A ) FITC-BSA-loaded liposomes and ( B ) FITC-BSA-loaded lipoparticles. ( C ) FITC-BSA-loaded liposomes and ( D ) FITC-BSA-loaded lipoparticles. The scale bar is 100 nm. ( E ) Particle size distribution of FITC-BSA-loaded lipoparticles containing a protamine/FITC-BSA core. Abbreviations: TEM, transmission <t>electron</t> microscope; FE-SEM, <t>field-emission</t> <t>scanning</t> electron microscope; FITC-BSA, fluorescein isothiocyanate-conjugated bovine serum albumin.
    Scanning Electron Microscopy, supplied by JEOL, used in various techniques. Bioz Stars score: 95/100, based on 10131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Hitachi Ltd scanning electron microscopy
    <t>SEM</t> images and EDS analysis of sintered ceramics. SEM images of sintered ceramics: ( a ) <t>AZO:Y</t> 0 , ( b ) AZO:Y 0.1 , ( c ) AZO:Y 0.15 , and ( d ) AZO:Y 0.2 . EDS analysis of a ( e ) white ZnAl 2 O 4 particle and ( f ) AZO:Y grain in the AZO:Y 0.2 ceramic sample.
    Scanning Electron Microscopy, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 95/100, based on 10758 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/scanning electron microscopy/product/Hitachi Ltd
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    92
    Hitachi Ltd transmission electron microscope
    <t>SEM</t> images and EDS analysis of sintered ceramics. SEM images of sintered ceramics: ( a ) <t>AZO:Y</t> 0 , ( b ) AZO:Y 0.1 , ( c ) AZO:Y 0.15 , and ( d ) AZO:Y 0.2 . EDS analysis of a ( e ) white ZnAl 2 O 4 particle and ( f ) AZO:Y grain in the AZO:Y 0.2 ceramic sample.
    Transmission Electron Microscope, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 92/100, based on 10725 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transmission electron microscope/product/Hitachi Ltd
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    95
    JEOL transmission electron microscopy tem
    (A) Picture showing 0.1% <t>CsA-loaded</t> NMF ( right ) in comparison to water ( left ). (B) <t>TEM</t> image for 0.1% CsA-loaded NMF ( scale : 500 nm).
    Transmission Electron Microscopy Tem, supplied by JEOL, used in various techniques. Bioz Stars score: 95/100, based on 13107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Carl Zeiss scanning electron microscope
    <t>SEM</t> images and fiber diameter distribution of <t>electrospun</t> nanofibers ( a ) MH ( b ) MHFSP1 ( c ) MHFSP3 ( d ) MHFSP5.
    Scanning Electron Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 4712 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Carl Zeiss scanning electron microscopy
    <t>SEM</t> images and fiber diameter distribution of <t>electrospun</t> nanofibers ( a ) MH ( b ) MHFSP1 ( c ) MHFSP3 ( d ) MHFSP5.
    Scanning Electron Microscopy, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 5089 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Philips Healthcare transmission electron microscope
    <t>SEM</t> images and fiber diameter distribution of <t>electrospun</t> nanofibers ( a ) MH ( b ) MHFSP1 ( c ) MHFSP3 ( d ) MHFSP5.
    Transmission Electron Microscope, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 6254 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    JEOL jem 1400 transmission electron microscope
    A super-low dose of LPS induces mitochondrial fission in murine macrophages. A , WT BMDMs were treated with a super-low dose of LPS (50 pg/ml) for 1 h and then labeled with MitoTracker Red to stain the mitochondria. The nuclei were stained using DAPI ( blue ). The cells were visualized under a Zeiss LSM510 laser-scanning confocal microscope (original magnification ×400). The merged images were magnified and are shown at the right. B , WT BMDMs were treated with 50 pg/ml LPS for 1 h. Cells were prepared and visualized under a JEOL <t>JEM</t> 1400 transmission electron microscope. The arrows denote fragmented mitochondria.
    Jem 1400 Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 94/100, based on 3736 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Philips Healthcare scanning electron microscope
    A super-low dose of LPS induces mitochondrial fission in murine macrophages. A , WT BMDMs were treated with a super-low dose of LPS (50 pg/ml) for 1 h and then labeled with MitoTracker Red to stain the mitochondria. The nuclei were stained using DAPI ( blue ). The cells were visualized under a Zeiss LSM510 laser-scanning confocal microscope (original magnification ×400). The merged images were magnified and are shown at the right. B , WT BMDMs were treated with 50 pg/ml LPS for 1 h. Cells were prepared and visualized under a JEOL <t>JEM</t> 1400 transmission electron microscope. The arrows denote fragmented mitochondria.
    Scanning Electron Microscope, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 2008 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Carl Zeiss transmission electron microscope
    A super-low dose of LPS induces mitochondrial fission in murine macrophages. A , WT BMDMs were treated with a super-low dose of LPS (50 pg/ml) for 1 h and then labeled with MitoTracker Red to stain the mitochondria. The nuclei were stained using DAPI ( blue ). The cells were visualized under a Zeiss LSM510 laser-scanning confocal microscope (original magnification ×400). The merged images were magnified and are shown at the right. B , WT BMDMs were treated with 50 pg/ml LPS for 1 h. Cells were prepared and visualized under a JEOL <t>JEM</t> 1400 transmission electron microscope. The arrows denote fragmented mitochondria.
    Transmission Electron Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 3031 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    JEOL jem 1010 transmission electron microscope
    Morphology of platelet and MK cells from the proband and a control. ( A ) Morphology of platelets in the bone marrow smears, which were stained with May-Grunwald Giemsa. The platelet in the proband is much larger than its normal size by reference to the red blood cells and to the control. The analysis was performed on an optical microscope (Leica DMR) with a 50×/1.40 numerical aperture oil objective lens (Leica). ( B ) Morphology and size (indicated by red arrows in the proband) of platelets from the peripheral blood observed by electronic microscopy (magnification of 10000×). ( C ) Morphology of a representative cultured MK from the bone marrow observed by electronic microscopy (magnification of 6000×). Ultrathin sections were examined by a <t>JEM-1010</t> transmission electron microscope (JEOL) at an accelerating voltage of 80 kV.
    Jem 1010 Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 94/100, based on 3129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    JEOL jem 1230 transmission electron microscope
    Phagosomal escape of F. tularensis . J774 cells were infected with F. tularensis at an MOI of 1,000 for 2 h and, after washing, incubated for another 6 h before they were fixed and analyzed by transmission electron microscopy (TEM). (A) Electron micrographs of infected J774 cells were acquired with a JEOL <t>JEM</t> 1230 Transmission Electron Microscope (JEOL Ltd., Tokyo, Japan). Black arrows indicate vacuolar membranes surrounding intracellular bacteria. (B) Bacteria were divided into one of 4 categories based on the membrane integrity of the surrounding vacuolar membrane. Micrographs in (A) illustrate the categories “Cytoplasm” (LVS and Δ iglE/E ) or “Intact phagosome” (Δ iglE and Δ iglC ).
    Jem 1230 Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 94/100, based on 3184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Field-emission scanning electron microscope morphologies of surfaces and cross sections of NBT thick films annealed at 750°C with various thicknesses.

    Journal: IEEE transactions on ultrasonics, ferroelectrics, and frequency control

    Article Title: Structure and Electrical Properties of Na0.5Bi0.5TiO3 Ferroelectric Thick Films Derived From a Polymer Modified Sol-Gel Method

    doi: 10.1109/TUFFC.2011.2054

    Figure Lengend Snippet: Field-emission scanning electron microscope morphologies of surfaces and cross sections of NBT thick films annealed at 750°C with various thicknesses.

    Article Snippet: The surface and cross-sectional morphologies of the NBT thick films were examined by a field-emission scanning electron microscope (FESEM, JEOL JSM-7000F, Tokyo, Japan).

    Techniques: Microscopy

    Field emission scanning electron micrographs of the fibres. The surface structure of the silk fibres derived from the non-transgenic silkworm lineages ( a ) and the transgenic silkworm lineages MASP1-2-1 ( b ), MASP1-12-5 ( c ) and MASP1-16-2 ( d ); the cross section structure of the silk fibres derived from the non-transgenic silkworm lineages ( a’ , a” ) and the transgenic silkworm lineages MASP1-2-1 (b’ , b” ), MASP1-12-5 ( c’ , c” ) and MASP1-16-2 ( d’ , d” ). Scale bars in a-d, 10μm; in a’-d’, 5 μm; in a”-d”, 1 μm.

    Journal: Scientific Reports

    Article Title: Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin

    doi: 10.1038/s41598-018-34150-y

    Figure Lengend Snippet: Field emission scanning electron micrographs of the fibres. The surface structure of the silk fibres derived from the non-transgenic silkworm lineages ( a ) and the transgenic silkworm lineages MASP1-2-1 ( b ), MASP1-12-5 ( c ) and MASP1-16-2 ( d ); the cross section structure of the silk fibres derived from the non-transgenic silkworm lineages ( a’ , a” ) and the transgenic silkworm lineages MASP1-2-1 (b’ , b” ), MASP1-12-5 ( c’ , c” ) and MASP1-16-2 ( d’ , d” ). Scale bars in a-d, 10μm; in a’-d’, 5 μm; in a”-d”, 1 μm.

    Article Snippet: The surface and cross sections of the fibres were placed on scanning electron microscopy stubs, coated with platinum at an accelerating voltage of 2 kV for two minutes, observed and photographed using a field emission scanning electron microscope (SU8010, Hitachi, Japan).

    Techniques: Derivative Assay, Transgenic Assay

    TEM image (left) and FE-SEM image (right) with 1% uranyl acetate negative staining. ( A ) FITC-BSA-loaded liposomes and ( B ) FITC-BSA-loaded lipoparticles. ( C ) FITC-BSA-loaded liposomes and ( D ) FITC-BSA-loaded lipoparticles. The scale bar is 100 nm. ( E ) Particle size distribution of FITC-BSA-loaded lipoparticles containing a protamine/FITC-BSA core. Abbreviations: TEM, transmission electron microscope; FE-SEM, field-emission scanning electron microscope; FITC-BSA, fluorescein isothiocyanate-conjugated bovine serum albumin.

    Journal: International Journal of Nanomedicine

    Article Title: The comparison of protein-entrapped liposomes and lipoparticles: preparation, characterization, and efficacy of cellular uptake

    doi: 10.2147/IJN.S25646

    Figure Lengend Snippet: TEM image (left) and FE-SEM image (right) with 1% uranyl acetate negative staining. ( A ) FITC-BSA-loaded liposomes and ( B ) FITC-BSA-loaded lipoparticles. ( C ) FITC-BSA-loaded liposomes and ( D ) FITC-BSA-loaded lipoparticles. The scale bar is 100 nm. ( E ) Particle size distribution of FITC-BSA-loaded lipoparticles containing a protamine/FITC-BSA core. Abbreviations: TEM, transmission electron microscope; FE-SEM, field-emission scanning electron microscope; FITC-BSA, fluorescein isothiocyanate-conjugated bovine serum albumin.

    Article Snippet: The morphology of the lipoparticles was examined using field emission-scanning electron microscopy (FE-SEM; JSM 6500F; JEOL).

    Techniques: Transmission Electron Microscopy, Negative Staining, Transmission Assay, Microscopy

    SEM images and EDS analysis of sintered ceramics. SEM images of sintered ceramics: ( a ) AZO:Y 0 , ( b ) AZO:Y 0.1 , ( c ) AZO:Y 0.15 , and ( d ) AZO:Y 0.2 . EDS analysis of a ( e ) white ZnAl 2 O 4 particle and ( f ) AZO:Y grain in the AZO:Y 0.2 ceramic sample.

    Journal: Nanoscale Research Letters

    Article Title: Nearly full-dense and fine-grained AZO:Y ceramics sintered from the corresponding nanoparticles

    doi: 10.1186/1556-276X-7-481

    Figure Lengend Snippet: SEM images and EDS analysis of sintered ceramics. SEM images of sintered ceramics: ( a ) AZO:Y 0 , ( b ) AZO:Y 0.1 , ( c ) AZO:Y 0.15 , and ( d ) AZO:Y 0.2 . EDS analysis of a ( e ) white ZnAl 2 O 4 particle and ( f ) AZO:Y grain in the AZO:Y 0.2 ceramic sample.

    Article Snippet: The morphology, microstructure, and composition analyses of the AZO:Y nanoparticles and the sintered bodies were performed using a scanning electron microscopy(SEM)/energy-dispersive X-ray analysis (EDAX) system (S-4800, Hitachi Ltd., Tokyo, Japan).

    Techniques:

    SEM images and the calculated particle sizes. Images of AZOY nanoparticles calcined at 600 °C for 2 h: ( a ) AZO:Y 0 , ( b ) AZO:Y 0.1 , ( c ) AZO:Y 0.15 , and ( d ) AZO:Y 0.2 . ( e ) The plot of the particle sizes calculated from ( a to d ) SEM images as a function of Y 2 O 3 content.

    Journal: Nanoscale Research Letters

    Article Title: Nearly full-dense and fine-grained AZO:Y ceramics sintered from the corresponding nanoparticles

    doi: 10.1186/1556-276X-7-481

    Figure Lengend Snippet: SEM images and the calculated particle sizes. Images of AZOY nanoparticles calcined at 600 °C for 2 h: ( a ) AZO:Y 0 , ( b ) AZO:Y 0.1 , ( c ) AZO:Y 0.15 , and ( d ) AZO:Y 0.2 . ( e ) The plot of the particle sizes calculated from ( a to d ) SEM images as a function of Y 2 O 3 content.

    Article Snippet: The morphology, microstructure, and composition analyses of the AZO:Y nanoparticles and the sintered bodies were performed using a scanning electron microscopy(SEM)/energy-dispersive X-ray analysis (EDAX) system (S-4800, Hitachi Ltd., Tokyo, Japan).

    Techniques:

    Field emission scanning electron microscopy (FE-SEM) images of the surface after (a) first anodization and second anodization for (b) 5 min, (c) 10 min, and (d) 20 min.

    Journal: ACS Omega

    Article Title: Preparation of a Highly Oleophobic Magnesium Alloy AZ31 Surface with Hierarchical Structure and Fluorination

    doi: 10.1021/acsomega.0c01225

    Figure Lengend Snippet: Field emission scanning electron microscopy (FE-SEM) images of the surface after (a) first anodization and second anodization for (b) 5 min, (c) 10 min, and (d) 20 min.

    Article Snippet: 4.6 CharacterizationThe surface structure was observed by field emission scanning electron microscopy (FE-SEM; SU6600, Hitachi, Japan).

    Techniques: Electron Microscopy

    (A) Picture showing 0.1% CsA-loaded NMF ( right ) in comparison to water ( left ). (B) TEM image for 0.1% CsA-loaded NMF ( scale : 500 nm).

    Journal: Translational Vision Science & Technology

    Article Title: Topical, Aqueous, Clear Cyclosporine Formulation Design for Anterior and Posterior Ocular Delivery

    doi: 10.1167/tvst.4.3.1

    Figure Lengend Snippet: (A) Picture showing 0.1% CsA-loaded NMF ( right ) in comparison to water ( left ). (B) TEM image for 0.1% CsA-loaded NMF ( scale : 500 nm).

    Article Snippet: Shapes and surface morphology of CsA-loaded NMF were determined with transmission electron microscopy (TEM) (JEOL JEM 1200 EX II Electron Microscope; Peabody, MA) using negative staining with uranyl acetate (UA).

    Techniques: Transmission Electron Microscopy

    SEM images and fiber diameter distribution of electrospun nanofibers ( a ) MH ( b ) MHFSP1 ( c ) MHFSP3 ( d ) MHFSP5.

    Journal: Medicina

    Article Title: Controlled Release of Metformin Hydrochloride from Core-Shell Nanofibers with Fish Sarcoplasmic Protein

    doi: 10.3390/medicina55100682

    Figure Lengend Snippet: SEM images and fiber diameter distribution of electrospun nanofibers ( a ) MH ( b ) MHFSP1 ( c ) MHFSP3 ( d ) MHFSP5.

    Article Snippet: Scanning Electron Microscopy (SEM) The morphologies of the electrospun fibers were examined using a scanning electron microscope(SEM) (EVA MA 10, Zeiss, San Diego, CA, USA) at an accelerating voltage of 10 kV.

    Techniques:

    Surface morphology of mesoporous zinc oxide (ZnO) structures obtained by Field-Emission Scanning Electron Microscope (FESEM): ( a ) top and cross-section views—scale bar is 1 μm; ( b ) FESEM image at higher magnification—scale bar is 100 nm; ( c ) X-ray diffraction pattern; ( d ) infrared spectrum.

    Journal: Materials

    Article Title: Gentamicin-Releasing Mesoporous ZnO Structures

    doi: 10.3390/ma11020314

    Figure Lengend Snippet: Surface morphology of mesoporous zinc oxide (ZnO) structures obtained by Field-Emission Scanning Electron Microscope (FESEM): ( a ) top and cross-section views—scale bar is 1 μm; ( b ) FESEM image at higher magnification—scale bar is 100 nm; ( c ) X-ray diffraction pattern; ( d ) infrared spectrum.

    Article Snippet: The morphology of the samples was evaluated by means of Field-Emission Scanning Electron Microscope (FESEM, Supra® 40, Carl Zeiss AG, Oberkochen, Germany).

    Techniques: Microscopy

    A super-low dose of LPS induces mitochondrial fission in murine macrophages. A , WT BMDMs were treated with a super-low dose of LPS (50 pg/ml) for 1 h and then labeled with MitoTracker Red to stain the mitochondria. The nuclei were stained using DAPI ( blue ). The cells were visualized under a Zeiss LSM510 laser-scanning confocal microscope (original magnification ×400). The merged images were magnified and are shown at the right. B , WT BMDMs were treated with 50 pg/ml LPS for 1 h. Cells were prepared and visualized under a JEOL JEM 1400 transmission electron microscope. The arrows denote fragmented mitochondria.

    Journal: The Journal of Biological Chemistry

    Article Title: Molecular and Cellular Mechanisms Responsible for Cellular Stress and Low-grade Inflammation Induced by a Super-low Dose of Endotoxin *

    doi: 10.1074/jbc.M114.569210

    Figure Lengend Snippet: A super-low dose of LPS induces mitochondrial fission in murine macrophages. A , WT BMDMs were treated with a super-low dose of LPS (50 pg/ml) for 1 h and then labeled with MitoTracker Red to stain the mitochondria. The nuclei were stained using DAPI ( blue ). The cells were visualized under a Zeiss LSM510 laser-scanning confocal microscope (original magnification ×400). The merged images were magnified and are shown at the right. B , WT BMDMs were treated with 50 pg/ml LPS for 1 h. Cells were prepared and visualized under a JEOL JEM 1400 transmission electron microscope. The arrows denote fragmented mitochondria.

    Article Snippet: Samples were sliced and prepared on grids for visualization on a JEOL JEM 1400 transmission electron microscope.

    Techniques: Labeling, Staining, Microscopy, Transmission Assay

    Morphology of platelet and MK cells from the proband and a control. ( A ) Morphology of platelets in the bone marrow smears, which were stained with May-Grunwald Giemsa. The platelet in the proband is much larger than its normal size by reference to the red blood cells and to the control. The analysis was performed on an optical microscope (Leica DMR) with a 50×/1.40 numerical aperture oil objective lens (Leica). ( B ) Morphology and size (indicated by red arrows in the proband) of platelets from the peripheral blood observed by electronic microscopy (magnification of 10000×). ( C ) Morphology of a representative cultured MK from the bone marrow observed by electronic microscopy (magnification of 6000×). Ultrathin sections were examined by a JEM-1010 transmission electron microscope (JEOL) at an accelerating voltage of 80 kV.

    Journal: PLoS ONE

    Article Title: A Missense Mutation in the Alpha-Actinin 1 Gene (ACTN1) Is the Cause of Autosomal Dominant Macrothrombocytopenia in a Large French Family

    doi: 10.1371/journal.pone.0074728

    Figure Lengend Snippet: Morphology of platelet and MK cells from the proband and a control. ( A ) Morphology of platelets in the bone marrow smears, which were stained with May-Grunwald Giemsa. The platelet in the proband is much larger than its normal size by reference to the red blood cells and to the control. The analysis was performed on an optical microscope (Leica DMR) with a 50×/1.40 numerical aperture oil objective lens (Leica). ( B ) Morphology and size (indicated by red arrows in the proband) of platelets from the peripheral blood observed by electronic microscopy (magnification of 10000×). ( C ) Morphology of a representative cultured MK from the bone marrow observed by electronic microscopy (magnification of 6000×). Ultrathin sections were examined by a JEM-1010 transmission electron microscope (JEOL) at an accelerating voltage of 80 kV.

    Article Snippet: The samples were then processed for electron microscopy according to standard procedures, and sections were analyzed using a JEOL JEM-1010 transmission electron microscope.

    Techniques: Staining, Microscopy, Cell Culture, Transmission Assay

    Phagosomal escape of F. tularensis . J774 cells were infected with F. tularensis at an MOI of 1,000 for 2 h and, after washing, incubated for another 6 h before they were fixed and analyzed by transmission electron microscopy (TEM). (A) Electron micrographs of infected J774 cells were acquired with a JEOL JEM 1230 Transmission Electron Microscope (JEOL Ltd., Tokyo, Japan). Black arrows indicate vacuolar membranes surrounding intracellular bacteria. (B) Bacteria were divided into one of 4 categories based on the membrane integrity of the surrounding vacuolar membrane. Micrographs in (A) illustrate the categories “Cytoplasm” (LVS and Δ iglE/E ) or “Intact phagosome” (Δ iglE and Δ iglC ).

    Journal: Virulence

    Article Title: A mutagenesis-based approach identifies amino acids in the N-terminal part of Francisella tularensis IglE that critically control Type VI system-mediated secretion

    doi: 10.1080/21505594.2016.1258507

    Figure Lengend Snippet: Phagosomal escape of F. tularensis . J774 cells were infected with F. tularensis at an MOI of 1,000 for 2 h and, after washing, incubated for another 6 h before they were fixed and analyzed by transmission electron microscopy (TEM). (A) Electron micrographs of infected J774 cells were acquired with a JEOL JEM 1230 Transmission Electron Microscope (JEOL Ltd., Tokyo, Japan). Black arrows indicate vacuolar membranes surrounding intracellular bacteria. (B) Bacteria were divided into one of 4 categories based on the membrane integrity of the surrounding vacuolar membrane. Micrographs in (A) illustrate the categories “Cytoplasm” (LVS and Δ iglE/E ) or “Intact phagosome” (Δ iglE and Δ iglC ).

    Article Snippet: Sections were viewed with a JEOL JEM 1230 Transmission Electron Microscope (JEOL Ltd., Tokyo, Japan).

    Techniques: Infection, Incubation, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Microscopy