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electrogenic nitric oxide reductase  (Federation of European Neuroscience Societies)
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sodium bicarbonate cotransporter nbc3  (Galbraith Laboratories Inc)
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electrogenic sodium-bicarbonate cotransporter 1  (Heermann Anesthesia Foundation)
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rabbit anti rat a 2 ar  (Alomone Labs)
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gene exp slc4a4 rn00584747 m1  (Thermo Fisher)
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Thermo Fisher gene exp slc4a4 rn00584747 m1
Quantitative RT-PCR data showing expression of: (A) maturation-phase ameloblast marker Klk4, and tight-junction protein genes Cldn1, Cldn4, Cldn8 (claudins 1, 4, and 8), and Tjp1 (ZO-1); (B) electrolyte transporter genes Slc9a1 (NHE1), Slc4a2 (AE2), <t>Slc4a4</t> (NBCe1), Slc26a4 (pendrin), and Cftr (CFTR). Expression levels in HAT-7 cells grown in Matrigel with Hepato-STIM medium (spheroid) were normalised to expression levels in HAT-7 cells grown on Transwell permeable supports in differentiation medium (monolayer). Data are mean ± SEM. * p < 0.05 (n = 4).
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electrogenic ion pumps  (Sinauer Associates Inc)
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Sinauer Associates Inc electrogenic ion pumps
Quantitative RT-PCR data showing expression of: (A) maturation-phase ameloblast marker Klk4, and tight-junction protein genes Cldn1, Cldn4, Cldn8 (claudins 1, 4, and 8), and Tjp1 (ZO-1); (B) electrolyte transporter genes Slc9a1 (NHE1), Slc4a2 (AE2), <t>Slc4a4</t> (NBCe1), Slc26a4 (pendrin), and Cftr (CFTR). Expression levels in HAT-7 cells grown in Matrigel with Hepato-STIM medium (spheroid) were normalised to expression levels in HAT-7 cells grown on Transwell permeable supports in differentiation medium (monolayer). Data are mean ± SEM. * p < 0.05 (n = 4).
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4070 tester  (Agilent technologies)
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Agilent technologies 4070 tester
Quantitative RT-PCR data showing expression of: (A) maturation-phase ameloblast marker Klk4, and tight-junction protein genes Cldn1, Cldn4, Cldn8 (claudins 1, 4, and 8), and Tjp1 (ZO-1); (B) electrolyte transporter genes Slc9a1 (NHE1), Slc4a2 (AE2), <t>Slc4a4</t> (NBCe1), Slc26a4 (pendrin), and Cftr (CFTR). Expression levels in HAT-7 cells grown in Matrigel with Hepato-STIM medium (spheroid) were normalised to expression levels in HAT-7 cells grown on Transwell permeable supports in differentiation medium (monolayer). Data are mean ± SEM. * p < 0.05 (n = 4).
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gene exp slc4a10 mm00473806 m1  (Thermo Fisher)
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Thermo Fisher gene exp slc4a10 mm00473806 m1
Quantitative RT-PCR data showing expression of: (A) maturation-phase ameloblast marker Klk4, and tight-junction protein genes Cldn1, Cldn4, Cldn8 (claudins 1, 4, and 8), and Tjp1 (ZO-1); (B) electrolyte transporter genes Slc9a1 (NHE1), Slc4a2 (AE2), <t>Slc4a4</t> (NBCe1), Slc26a4 (pendrin), and Cftr (CFTR). Expression levels in HAT-7 cells grown in Matrigel with Hepato-STIM medium (spheroid) were normalised to expression levels in HAT-7 cells grown on Transwell permeable supports in differentiation medium (monolayer). Data are mean ± SEM. * p < 0.05 (n = 4).
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electrogel  (electro cap international)
90
electro cap international electrogel
Quantitative RT-PCR data showing expression of: (A) maturation-phase ameloblast marker Klk4, and tight-junction protein genes Cldn1, Cldn4, Cldn8 (claudins 1, 4, and 8), and Tjp1 (ZO-1); (B) electrolyte transporter genes Slc9a1 (NHE1), Slc4a2 (AE2), <t>Slc4a4</t> (NBCe1), Slc26a4 (pendrin), and Cftr (CFTR). Expression levels in HAT-7 cells grown in Matrigel with Hepato-STIM medium (spheroid) were normalised to expression levels in HAT-7 cells grown on Transwell permeable supports in differentiation medium (monolayer). Data are mean ± SEM. * p < 0.05 (n = 4).
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Image Search Results


Quantitative RT-PCR data showing expression of: (A) maturation-phase ameloblast marker Klk4, and tight-junction protein genes Cldn1, Cldn4, Cldn8 (claudins 1, 4, and 8), and Tjp1 (ZO-1); (B) electrolyte transporter genes Slc9a1 (NHE1), Slc4a2 (AE2), Slc4a4 (NBCe1), Slc26a4 (pendrin), and Cftr (CFTR). Expression levels in HAT-7 cells grown in Matrigel with Hepato-STIM medium (spheroid) were normalised to expression levels in HAT-7 cells grown on Transwell permeable supports in differentiation medium (monolayer). Data are mean ± SEM. * p < 0.05 (n = 4).

Journal: Frontiers in Pharmacology

Article Title: Three-Dimensional Culture of Ameloblast-Originated HAT-7 Cells for Functional Modeling of Defective Tooth Enamel Formation

doi: 10.3389/fphar.2021.682654

Figure Lengend Snippet: Quantitative RT-PCR data showing expression of: (A) maturation-phase ameloblast marker Klk4, and tight-junction protein genes Cldn1, Cldn4, Cldn8 (claudins 1, 4, and 8), and Tjp1 (ZO-1); (B) electrolyte transporter genes Slc9a1 (NHE1), Slc4a2 (AE2), Slc4a4 (NBCe1), Slc26a4 (pendrin), and Cftr (CFTR). Expression levels in HAT-7 cells grown in Matrigel with Hepato-STIM medium (spheroid) were normalised to expression levels in HAT-7 cells grown on Transwell permeable supports in differentiation medium (monolayer). Data are mean ± SEM. * p < 0.05 (n = 4).

Article Snippet: Amplification was performed using QuantStudio 5 with TaqPath qPCR Master Mix, CG (Applied Biosystems, Foster City, CA, United States) and predesigned primers (Life Technologies Magyarország Kft., Budapest): Klk4 (Rn01498536_m1), Cldn1 (Rn00581740_m1), Cldn4 (Rn01196224_s1), Cldn8 (Rn01767199_s1), Tjp1/ZO-1 (Rn02116071_s1), Slc4a2/AE2 (Rn00566910_m1), Slc4a4/NBCe1 (Rn00584747_m1), Slc9a1/NHE1 (Rn00561924_m1), CFTR (Rn01455971_m1), Slc26a4/pendrin (Rn00693043_m1).

Techniques: Quantitative RT-PCR, Expressing, Marker