Journal: Advanced Science

Article Title: The Hemagglutinin of Influenza A Virus Induces Ferroptosis to Facilitate Viral Replication

doi: 10.1002/advs.202404365

Figure Lengend Snippet: Lipid peroxidation resulting from IAV hemagglutinin impairs MAVS‐mediated antiviral immunity. A) The IFNβ promoter activity induced by MAVS in HEK293T WT cells or NCOA4 KO cells, with or without transfection of PR8 HA. HEK293 WT or KO cells were transfected with vector or PR8 HA and MAVS, followed by IFNβ promoter activity determined by luciferase assay. B–D) The effects of PR8 HA on MAVS‐induced IFNβ promoter activity with and without lipid peroxidation inhibition. HEK293T cells were transfected with either vector or PR8 HA and MAVS. After 6 h post‐transfection, the cells were treated with NCOA4‐9a (2.5 µ m ), DFO (100 µ m ), or Fer‐1 (5 µ m ) for 24 h. The IFNβ promoter activity was detected by luciferase assay. E) Western blotting analysis was performed to investigate the impact of PR8 HA on MAVS aggregates. HEK293T cells were co‐transfected with vector or HA‐HA PR8 and Flag‐MAVS in HEK293T cells, and cell lysates were harvested at 24 h for SDS‐PAGE and SDD‐AGE, followed by western blotting analysis. F) Western blotting analysis of the effect of PR8 HA for MAVS aggregates in NCOA4 KO cells. In HEK293T WT or NCOA4 KO cells, vector or HA‐HA PR8 and Flag‐MAVS were co‐transfected separately, and cell lysates were harvested at 24 h post‐transfection for SDD‐AGE vs SDS‐PAGE, followed by western blotting analysis. G‐I) The effects of PR8 HA on MAVS aggregates with and without lipid peroxidation inhibition. HEK293T cells were transfected with either vector or PR8 HA and MAVS. After 6 h of transfection, the cells were treated with NCOA4‐9a (2.5 µ m ), DFO (100 µ m ) or Fer‐1 (5 µ m ) for 24 h. Cell lysates were harvested at 24 h post‐transfection for SDD‐AGE vs SDS‐PAGE, followed by western blotting analysis. J) 4‐HNE levels were analyzed by western blotting in HEK293T cells transfected with 1, 2, and 4 µg of PR8 HA. K‐M) HEK293T cells were transfected with Flag‐MAVS, followed by treatment with 0, 5, 10 and 20 µ m 4‐HNE for 12 h. Cell lysates were harvested for analysis. K) The IFNβ promoter activity induced by 4‐HNE on MAVS was determined by luciferase assay. L) The effects of 4‐HNE on MAVS aggregates were analyzed by western blotting. M) The ρ‐IRF3 level induced by 4‐HNE on MAVS was analyzed by western blotting. Data were shown as mean ± SEM ( n = 3) from triplicate independent experiments, and significance was analyzed by two‐tailed Student's t‐test. (** p < 0.01; *** p < 0.001; ns, no significant).

Article Snippet: The inhibitors and stimulators used in this study were as follows: RSL3 (HY‐100218A), Ferrostatin‐1 (Fer‐1, HY‐100579), Liproxstatin‐1 (Lip‐1, HY‐12726), Deferoxamine mesylate (DFO, HY‐B0988), Chloroquine (CQ, HY‐17589A), and Polyinosinic‐polycytidylic acid (Poly (I:C), HY‐107202) were MCE; NCOA4‐9a (2650557‐72‐3), and 4‐Hydroxynonenal (4‐HNE, 75899‐68‐2) were from TargetMol.

Techniques: Activity Assay, Transfection, Plasmid Preparation, Luciferase, Inhibition, Western Blot, SDS Page, Two Tailed Test

Journal: iScience

Article Title: Deciphering decarbonization trajectories in China by spatiotemporal-accumulation modeling of electricity carbon footprint

doi: 10.1016/j.isci.2025.111963

Figure Lengend Snippet: Qualitatively describe three scenarios of indirect greenhouse gas emissions (GHG ele ) from cradle-to-gate power consumption in LCPI component production

Article Snippet: In order to quantify the synergistic decarbonization behavior between LCPI manufacturing and the power grid under the cumulative effect of heterogenous-embodied-carbon devices, we have defined two comparison benchmarks for validating the proposed model. As shown in , the first benchmark (BS1) is based on the constant GHG ele as reported in Ecoinvent V3.7, where results are only influenced by the energy structure of the power grid.

Techniques: Expressing

Journal: Nature Communications

Article Title: Integrated 3D printing of flexible electroluminescent devices and soft robots

doi: 10.1038/s41467-022-32126-1

Figure Lengend Snippet: a Schematic illustration showing the multi-material direct ink writing of flexible EL devices. The electroluminescent devices consist of an electroluminescent elastomer (ELE) layer and an insulating dielectric elastomer (IDE) layer sandwiched between two ion conducting elastomer (ICE) layers. The robust interface is built through the covalent bonds. b Schematic illustration of the electroluminescent soft robot (ELbot) featuring chameleon-like adaptiveness to external light variation.

Article Snippet: Both devices consisted of a ELE layer (with “SUSTech” logo) and an ICE layer.

Techniques:

Journal: Nature Communications

Article Title: Integrated 3D printing of flexible electroluminescent devices and soft robots

doi: 10.1038/s41467-022-32126-1

Figure Lengend Snippet: a Schematic illustration of the multi-material printing process for fabricating an EL device, which consisted of two ion conducting elastomer (ICE) layers and one electroluminescent elastomer (ELE) layer. The addition of SiO 2 nanoparticles in the inks leads to physically-crosslinked gels, which are fluidized by the shearing force during ink extrusion and can be recovered to the gel state right after printing. Further UV-initiated polymerization of DMAPS/PEGDA or TEAc/PEGDA leads to the formation of an intact network structure. b Images of the 3DP EL devices under mechanical deformation (i.e., twisting and bending), which are powered with an alternating current. Scale bar: 5 mm. c Shear storage moduli ( G ') and loss moduli ( G '') of the ICE, ELE, and IDE inks as a function of shear stress at 25 ∘ C, measured under an oscillatory mode at a frequency of 1 Hz. d Apparent viscosity of the optimized ICE, ELE, and IDE inks as a function of shear rate at 25 ∘ C. e Images of the high-fidelity electroluminescent prints of the ELE patterns. f Summary of the physical parameters (i.e., modulus ( E ), strength ( S ), elongation at break ( λ ) and conductivity ( σ )) of the ICE samples fabricated from both molding and the 3D printing techniques. g Plotting of the luminance intensity of the 3DP ELE patterns using inks with or without SiO 2 NPs against the applied voltage per thickness ( E ) (thickness of ELE layer was 150 μm, frequency of the applied voltage was 500 Hz). h Plotting of the luminance intensity of the 3DP ELE patterns against the applied voltage per thickness ( E ) at various frequencies of the applied voltage. Data in f , g , and h are means ± S.D., n = 3 independent samples.

Article Snippet: Both devices consisted of a ELE layer (with “SUSTech” logo) and an ICE layer.

Techniques: Shear, Viscosity

Journal: Nature Communications

Article Title: Integrated 3D printing of flexible electroluminescent devices and soft robots

doi: 10.1038/s41467-022-32126-1

Figure Lengend Snippet: a Schematic illustration of the multi-material DIW process for fabricating EL devices. ICE, IDE and ELE Inks were printed sequentially. b – d SEM images of the multi-layer structure of the 3DP samples ( b ), the interface between the ICE and ELE layers ( c ), and the interface between two ICE layers ( d ) of the 3DP samples. Scale bar: 200 μm ( b ) and 50 μm ( c , d ). e Simulated displacement distributions within the 3DP EL device (top) and a physically-laminated sample (bottom) at an overall applied strain of 35%. Both devices consisted of a ELE layer (with “SUSTech” logo) and an ICE layer. The simulation was performed via finite element analysis. Mechanically compliant and robust interface was evidenced in the 3DP EL device, while weak interface and interfacial delamination were detected for the physically-laminated EL device. f The designated CAD models and the images of the 3DP EL devices with multi-color electroluminescent components with alternative voltage on or off. Scale bar: 5 mm. g Images of a 3DP flexible EL wristband, indicating the stable electroluminescent features under mechanical deformation, such as bending and twisting. Scale bar: 1 cm.

Article Snippet: Both devices consisted of a ELE layer (with “SUSTech” logo) and an ICE layer.

Techniques: