Journal: bioRxiv

Article Title: Cell type–resolved transcriptomic map of skeletal muscle in women with polycystic ovary syndrome

doi: 10.64898/2026.03.13.711523

Figure Lengend Snippet: A) A fixed 5-plex panel outlines different cell types in skeletal muscle: M-I fibers (TNNC1, yellow), M-II fibers (MYH2, purple), satellite cells (PAX7, cyan), fibroblasts (FBLN2, red), and endothelial cells (CD34, white). B-C) Multiplex staining of five different DEGs is visualized in green (COL4A3, COL15A1, ELN, LAMB1, and XIRP2), with the top panels showing only the DEG together with DAPI, and bottom panels displaying all seven channels (5-plex panel, DEG, and DAPI). Representative images are shown for control samples, PCOS patients before treatment (PCOS week 0), and PCOS patients after 16 weeks of metformin treatment.

Article Snippet: Primary antibodies for the DEGs were: COL4A2 (C1926, Sigma-Aldrich), COL15A1 (HPA017913, Atlas Antibodies AB), ELN (HPA018111, Atlas Antibodies AB), LAMB1 (sc-17763, Santa Cruz Biotechnology, Dallas, TX), and XIRP2 (HPA074599, Atlas Antibodies AB).

Techniques: Multiplex Assay, Staining, Control

Journal: European Spine Journal

Article Title: Platelet-rich plasma induces annulus fibrosus cell proliferation and matrix production

doi: 10.1007/s00586-014-3198-x

Figure Lengend Snippet: Oligonucleotide primers and probes and Gene Expression Assays (from Applied Biosystems) used for real-time PCR; fw, forward; rev, reverse; serial number is indicated for Gene Expression Assays

Article Snippet: Primer fw 5′–3′ Primer rev 5′–3′ Probe (5′FAM/3′TAMRA) Glyceraldehyde-3-phosphate dehydrogenase GAPDH GGC TGC TTT TAA TTC TGG CAA A AAT CAT ACT GGA ACA TGT AGA CCA TGT A TGG ACA TCG TCG CCA TCA ATG ACC Collagen type I COL1A2 TGC AGT AAC TTC GTG CCT AGC A CGC GTG GTC CTC TAT CTC CA CAT GCC AAT CCT TAC AAG AGG CAA CTG Collagen type II COL2A1 AAG AAA CAC ATG TGG TTT GGA GAA A TGG GAG CCA GGT TGT CAT CAA CGG TGG CTT CCA CTT CAG CTA TGG Aggrecan ACAN CCA ACG AAA CCT ATG ACG TGT ACT GCA CTC GTT GGC TGC CTC ATG TTG CAT AGA AGA CCT CGC CCT CCA T Biglycan BGN Bt03244532_m1 Decorin DCN Bt03230914_m1 Elastin ELN Bt03216594_m1 Versican VCN Bt03217632_m1 Open in a separate window Oligonucleotide primers and probes and Gene Expression Assays (from Applied Biosystems) used for real-time PCR; fw, forward; rev, reverse; serial number is indicated for Gene Expression Assays Whole disc organ culture model Application of platelet preparation was further tested in an organ culture model. PRP was chosen for the organ culture experiment, because injection of PL could not generate reproducible results, due to repeated leakage of PL from the AF defect.

Techniques: Gene Expression, Real-time Polymerase Chain Reaction