Journal: bioRxiv

Article Title: Melanocore uptake by keratinocytes occurs through phagocytosis and involves Protease-activated receptor-2 activation

doi: 10.1101/2021.04.13.439501

Figure Lengend Snippet: XB2 keratinocytes were transfected with non-targeting siRNA (siCtrl), siRNA targeting PAR-2 (siPAR-2), Rac1 (siRac1) or BARS (siBARS) (A and B) and treated or not with EHT 1864 (EHT) or EIPA (C and D). Cells were then incubated with 0.1 g/l of melanocores. The transfected cells and the cells treated with the drugs were fixed 24 hours or 2 hours later, respectively and examined by brightfield microscopy. Nuclei were visualized by DAPI staining (blue). Scale bars = 10 μm. (B) Quantification of internalized melanocores/cell after a 24h pulse. (D) Quantification of internalized melanocores/cell after a 2h pulse. p values (unpaired Student t-test) were considered statistically significant when <0.05 (*), <0.01 (**), <0.001 (***) or non-significant (n.s.) when >0.05. Plots show mean ± SD of three independent experiments.

Article Snippet: In the assays using inhibitors, cells were incubated with EIPA (Tocris, MN, USA; 50 μM) or EHT 1864 (Santa Cruz Biotechnology, Dallas, TX, USA; 20 μM) for 20 minutes or overnight, respectively, before being fed with melanocores (0.1 g/l), melanosomes or Rhodamine B isothiocyanate–Dextran 70kDa (Sigma, Darmstadt, Germany; 0.1μg/μl) for 2h and processed for immunofluorescence as described below.

Techniques: Transfection, Incubation, Microscopy, Staining

Journal: bioRxiv

Article Title: Melanocore uptake by keratinocytes occurs through phagocytosis and involves Protease-activated receptor-2 activation

doi: 10.1101/2021.04.13.439501

Figure Lengend Snippet: XB2 keratinocytes were transfected with non-targeting siRNA (siCtrl), siRNA targeting PAR-2 (siPAR-2), Rac1 (siRac1) or BARS (siBARS) (A and B) and treated or not with EHT 1864 (EHT) or EIPA (C and D). Cells were then incubated with 0.1 g/l of melanosomes. The transfected cells and the cells treated with the drugs were fixed 24 hours or 2 hours later, respectively and examined by brightfield microscopy. Nuclei were visualized by DAPI staining (blue). Scale bars = 10 μm. (B) Quantification of internalized melanosomes/cell after a 24h pulse. (D) Quantification of internalized melanosomes/cell after a 2h pulse. p values (unpaired Student t-test) were considered statistically significant when <0.05 (*), <0.01 (**), <0.001 (***) or non-significant (n.s.) when >0.05. Plots show mean ± SD of three independent experiments.

Article Snippet: In the assays using inhibitors, cells were incubated with EIPA (Tocris, MN, USA; 50 μM) or EHT 1864 (Santa Cruz Biotechnology, Dallas, TX, USA; 20 μM) for 20 minutes or overnight, respectively, before being fed with melanocores (0.1 g/l), melanosomes or Rhodamine B isothiocyanate–Dextran 70kDa (Sigma, Darmstadt, Germany; 0.1μg/μl) for 2h and processed for immunofluorescence as described below.

Techniques: Transfection, Incubation, Microscopy, Staining

Journal: eLife

Article Title: Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis

doi: 10.7554/eLife.76664

Figure Lengend Snippet:

Article Snippet: Chemical compound, drug , EIPA , Cayman Chemical , Cat#14406; CAS:1154-25-2 , .

Techniques: Generated, Control, Cell Culture, Staining, Software

Journal: Pharmaceutics

Article Title: Modified PEG-Lipids Enhance the Nasal Mucosal Immune Capacity of Lipid Nanoparticle mRNA Vaccines

doi: 10.3390/pharmaceutics16111423

Figure Lengend Snippet: Function and Concentration of Endocytosis Inhibitors.

Article Snippet: Nystatin, chlorpromazine hydrochloride, and ethylisopropylamiloride (EIPA) were purchased from Solarbio Life Sciences (Beijing, China).

Techniques: Concentration Assay, Inhibition

Journal: ACS Omega

Article Title: Development of a Simultaneous Analytical Method for Amines Corresponding to 10 Typical Nitrosamines

doi: 10.1021/acsomega.4c06293

Figure Lengend Snippet: MS chromatograms of 0.2 μg/mL of each amine standard solution (top) and the blank solution (bottom). (A), (B), (C), (D), (E), (F), (G), (H), (I), and (J) are chromatograms of MeP, DMA, MOR, MBA, DEA, EIPA, DIPA, DPA, MPA, and DBA, respectively.

Article Snippet: DMA, EIPA, DIPA, MeP, and MOR were procured from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan).

Techniques:

Journal: ACS Omega

Article Title: Development of a Simultaneous Analytical Method for Amines Corresponding to 10 Typical Nitrosamines

doi: 10.1021/acsomega.4c06293

Figure Lengend Snippet: (A), (B), (C), (D), (E), (F), (G), (H), (I), and (J) are linearity of MeP, DMA, MOR, MBA, DEA, EIPA, DIPA, DPA, MPA, and DBA, respectively.

Article Snippet: DMA, EIPA, DIPA, MeP, and MOR were procured from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan).

Techniques:

Journal: ACS Omega

Article Title: Development of a Simultaneous Analytical Method for Amines Corresponding to 10 Typical Nitrosamines

doi: 10.1021/acsomega.4c06293

Figure Lengend Snippet: MS chromatograms of each amine at LOD concentrations. (A–J) Chromatograms of MeP, DMA, MOR, MBA, DEA, EIPA, DIPA, DPA, MPA, and DBA. MeP, MOR, MBA, DEA, EIPA, DIPA, and DPA are at 0.001 μg/mL, and DMA, MPA, and DBA are at 0.003 μg/mL.

Article Snippet: DMA, EIPA, DIPA, MeP, and MOR were procured from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan).

Techniques:

Journal: ACS Omega

Article Title: Development of a Simultaneous Analytical Method for Amines Corresponding to 10 Typical Nitrosamines

doi: 10.1021/acsomega.4c06293

Figure Lengend Snippet: Repeatability Results of Each Amine Solution at the LOQ Concentration

Article Snippet: DMA, EIPA, DIPA, MeP, and MOR were procured from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan).

Techniques:

Journal: ACS Omega

Article Title: Development of a Simultaneous Analytical Method for Amines Corresponding to 10 Typical Nitrosamines

doi: 10.1021/acsomega.4c06293

Figure Lengend Snippet: Repeatability Results of Each Amine Solution at 0.2 μg/mL Concentration

Article Snippet: DMA, EIPA, DIPA, MeP, and MOR were procured from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan).

Techniques:

Journal: ACS Omega

Article Title: Development of a Simultaneous Analytical Method for Amines Corresponding to 10 Typical Nitrosamines

doi: 10.1021/acsomega.4c06293

Figure Lengend Snippet: Numbers of APIs with Each Amine Detected

Article Snippet: DMA, EIPA, DIPA, MeP, and MOR were procured from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan).

Techniques:

Journal: ACS Omega

Article Title: Development of a Simultaneous Analytical Method for Amines Corresponding to 10 Typical Nitrosamines

doi: 10.1021/acsomega.4c06293

Figure Lengend Snippet: MS Transitions of Each Amine

Article Snippet: DMA, EIPA, DIPA, MeP, and MOR were procured from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan).

Techniques: