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BioTherapeutics Inc
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Promega
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Image Search Results
Journal: Cell reports
Article Title: De novo recovery of Ghana virus, an African bat Henipavirus, reveals differential tropism and attenuated pathogenicity compared to Nipah virus
doi: 10.1016/j.celrep.2026.117074
Figure Lengend Snippet: (A–E) Dose-response curves are shown for various small-molecule inhibitors against rGhV M-GS* (purple) and rNiV (orange). CHO-B2 cells were infected at an MOI of 0.05 and treated with (A) EIDD-2749, (B) GHP-88309, (C) remdesivir, (D) EIDD-1931 (molnupiravir active form), or (E) favipiravir (T-705). (F and G) Additional dose-response curves were measured in primary human astrocytes for (F) EIDD-2749 and (G) GHP-88309 under the same MOI and assay conditions. (H) Summary of half-maximal inhibitory concentrations (IC 50 ) derived from the dose-response curves in (A–G). Each row indicates the respective compound, virus, calculated IC 50 , corresponding 95% confidence interval, and the cell type in which the assay was performed. IC 50 values were determined in GraphPad Prism by nonlinear regression of (inhibitor) vs. normalized response. All experiments were conducted in biological triplicate. Error bars depict standard deviation.
Article Snippet:
Techniques: Infection, Derivative Assay, Virus, Standard Deviation
Journal: bioRxiv
Article Title: Lassa virus NP DEDDh 3’-5’ exoribonuclease activity is required for optimal viral RNA replication
doi: 10.1101/2023.04.12.536665
Figure Lengend Snippet: (A). Vero cells were treated with 5’-FU and EIDD-1931 at indicated concentrations. At 48 hr post treatment, cell viability was measured using CellTiter-Glo Viability Assay (Promega). Data shown are the average (n=4) and SEM. (B) and (C). Vero cells were treated with 5-FU and EIDD-1931 at different concentrations as indicated. Cells were infected with wt rLASV (wt) and ExoN-rLASV (ExoN-) at MOI 0.1. At 48 hpi, virus titers were determined by plaque assay. Log10 virus titer changes relative to the virus titer of non-treated cells are shown. Data presented are the mean and the SEM of three independent experiments.
Article Snippet: Treatment of Vero cells with
Techniques: Viability Assay, Infection, Virus, Plaque Assay