Journal: Tissue Engineering and Regenerative Medicine

Article Title: Fabrication of Functional Cell Sheets with Human Thyrocytes from Non-Tumorous Thyroid Tissue

doi: 10.1007/s13770-019-00198-8

Figure Lengend Snippet: List of TaqMan ® gene expression assay mix (20x)/PCR primers used in RT-qPCR studies

Article Snippet: The conditions for the reactions were set at 95 °C for 20 s and 40 cycles at 95 °C for 1 s and 60 °C for 20 s. The cycle threshold values were determined automatically by the Applied Biosystems StepOnePlus Real-Time PCR System, and the fold-change in the gene expression was calculated by the 2 −ΔΔCT method. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Gene symbol Gene name Assay ID Species Amplicon length TPO Thyroid peroxidase Hs00892519_m1 Human 53 TG Thyroglobulin Hs00174974_m1 Human 69 TGFB Transforming growth factor beta 1 Hs00998133_m1 Human 57 IL6 Interleukin 6 Hs00985639_m1 Human 66 EGF Epidermal growth factor Hs01099999_m1 Human 70 Open in a separate window List of TaqMan ® gene expression assay mix (20x)/PCR primers used in RT-qPCR studies Fabrication of cell sheets Human primary thyroid cell sheets were constructed using TRCDs.

Techniques: Gene Expression, Amplification

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Fabrication of Functional Cell Sheets with Human Thyrocytes from Non-Tumorous Thyroid Tissue

doi: 10.1007/s13770-019-00198-8

Figure Lengend Snippet: Changes in the gene expression of thyroid cell markers and cytokines. A-E RT-qPCR analyses of TPO, TG, IL-6, TGFB and EGF for cells cultured in DMEM and HDM. GAPDH was set as the housekeeper gene. TPO: thyroid peroxidase; TG: thyroglobulin; TGFB: transforming growth factor-β; EGF: epidermal growth factor. *p < 0.05, **p < 0.01, **p < 0.01, ***p < 0.001 (two-way ANOVA), n.s.: non significance, n = 6 in 2 cases

Article Snippet: The conditions for the reactions were set at 95 °C for 20 s and 40 cycles at 95 °C for 1 s and 60 °C for 20 s. The cycle threshold values were determined automatically by the Applied Biosystems StepOnePlus Real-Time PCR System, and the fold-change in the gene expression was calculated by the 2 −ΔΔCT method. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Gene symbol Gene name Assay ID Species Amplicon length TPO Thyroid peroxidase Hs00892519_m1 Human 53 TG Thyroglobulin Hs00174974_m1 Human 69 TGFB Transforming growth factor beta 1 Hs00998133_m1 Human 57 IL6 Interleukin 6 Hs00985639_m1 Human 66 EGF Epidermal growth factor Hs01099999_m1 Human 70 Open in a separate window List of TaqMan ® gene expression assay mix (20x)/PCR primers used in RT-qPCR studies Fabrication of cell sheets Human primary thyroid cell sheets were constructed using TRCDs.

Techniques: Gene Expression, Quantitative RT-PCR, Cell Culture