Journal: Arthritis research & therapy

Article Title: Epigallocatechin-3-gallate suppresses the global interleukin-1beta-induced inflammatory response in human chondrocytes.

doi: 10.1186/ar3368

Figure Lengend Snippet: Figure 1 Inhibition of IL-1b induced cytokine expression in human OA chondrocytes by EGCG (a, b). Chondrocytes were pretreated for 2 h with EGCG (100 μM) and then stimulated or not stimulated with IL-1b (5 ng/ml) for 24 h. Values represent Mean ± SE of three different patients. # represents P < 0.05 Vs control; * P < 0.05 Vs IL-1b stimulated chondrocytes. EGCG, epigallocatechin-3-gallate; GCSF, granulocyte colony stimulating factor; GM-CSF, granulocyte-macrophages colony stimulating factor; IFN-g, interferon-g; IL, interleukin; LIF, leukemia inhibitory factor; MCSF, macrophages colony stimulating factor; MIF, macrophage migratory inhibitory factor; TNF, tumor necrosis factor.

Article Snippet: Enzyme linked immune-sorbant assays (ELISA) The effect of IL-1b and/or EGCG (100 μM) on the level of IL-6, IL-8 and TNF-a secreted by chondrocytes in the culture medium was further confirmed by ELISAs (R&D Systems, Minneapolis, MN, USA) and all the assays were performed according to the manufacturer’s instructions.

Techniques: Inhibition, Expressing, Control

Journal: Arthritis research & therapy

Article Title: Epigallocatechin-3-gallate suppresses the global interleukin-1beta-induced inflammatory response in human chondrocytes.

doi: 10.1186/ar3368

Figure Lengend Snippet: Figure 2 Effect of EGCG on IL-1b stimulated secretion of chemokines in human OA chondrocytes (a-b). Chondrocytes were pretreated for 2 h with EGCG (100 μM) and then stimulated or not stimulated with IL-1b (5 ng/ml) for 24 h. Values represent Mean ± SE of three different patients. # represents P < 0.05 Vs control; * P < 0.05 Vs IL-1b stimulated chondrocytes. BLC, B-lymphocyte chemoattractant; EGCG, epigallocatechin-3-gallate; ENA-78, epithelial neutrophil activating peptide-78; GCP-2, granulocyte chemotactic protein-2; GRO, growth-related oncogene; IL- interleukin; MCP, monocyte chemotactic protein; MDC, macrophage-derived chemokine; MIG, monokine induced by interferon- gamma; MIP, macrophage inflammatory protein; NAP-2, nucleosome assembly protein-2; RANTES, regulated upon activation normal T-cell expressed and secreted;IP-10,10 kDa interferon gamma-induced protein.

Article Snippet: Enzyme linked immune-sorbant assays (ELISA) The effect of IL-1b and/or EGCG (100 μM) on the level of IL-6, IL-8 and TNF-a secreted by chondrocytes in the culture medium was further confirmed by ELISAs (R&D Systems, Minneapolis, MN, USA) and all the assays were performed according to the manufacturer’s instructions.

Techniques: Control, Derivative Assay, Activation Assay

Journal: Arthritis research & therapy

Article Title: Epigallocatechin-3-gallate suppresses the global interleukin-1beta-induced inflammatory response in human chondrocytes.

doi: 10.1186/ar3368

Figure Lengend Snippet: Figure 3 Effect of EGCG on secretion of growth and angiogenic factors in human OA chondrocytes stimulated with IL-1b (a, b). Chondrocytes were pretreated for 2 h with EGCG (100 μM) and then stimulated or not stimulated with IL-1b (5 ng/ml) for 24 h. Values represent Mean ± SE of three different patients. # represents P < 0.05 Vs control; * P < 0.05 Vs IL-1b stimulated chondrocytes. BDNF, brain- derived neurotrophic factor; EGCG, epigallocatechin-3-gallate; EGF, endothelial growth factor; FGF, fibroblast growth factor; GDNF, glial cell derived neurotrophic factor; HGF, hepatocyte growth factor; IGF, insulin-like growth factor; IGFBP, insulin-like growth factor binding protein; NT, neurotrophin; PDGF-BB, platelet-derived growth factor receptor, beta polypeptide subunit B; PIGF, phosphatidylinositol glycan anchor biosynthesis; TGF, transforming growth factor; TIMP, tissue inhibitor of MMPs; VEGF, vascular endothelial growth factor.

Article Snippet: Enzyme linked immune-sorbant assays (ELISA) The effect of IL-1b and/or EGCG (100 μM) on the level of IL-6, IL-8 and TNF-a secreted by chondrocytes in the culture medium was further confirmed by ELISAs (R&D Systems, Minneapolis, MN, USA) and all the assays were performed according to the manufacturer’s instructions.

Techniques: Control, Derivative Assay, Binding Assay, Glycoproteomics

Journal: Arthritis research & therapy

Article Title: Epigallocatechin-3-gallate suppresses the global interleukin-1beta-induced inflammatory response in human chondrocytes.

doi: 10.1186/ar3368

Figure Lengend Snippet: Figure 4 Effect of EGCG IL-6, IL-8 and TNF-a gene expression and production in IL-1b-stimulated human OA chondrocytes. Chondrocytes were pretreated with EGCG (10 to 100 μM) for 2 h and stimulated with IL-1b for (a, b) 8 h or (c, d, e, and f) 24 h. Gene expression for IL-6, IL-8 and TNF-a was determined by quantitative RT-PCR normalized to GAPDH and then compared with the levels present in un-stimulated chondrocytes. Levels of IL-6, IL-8 and TNF-a in the culture supernatants were quantified by sandwich ELISA. Value represents Mean ± SE of three different patients. # represents P < 0.05 Vs control; * P < 0.05 Vs IL-1b stimulated chondrocytes. EGCG, epigallocatechin-3-gallate; IL-interleukin; TNF, tumor necrosis factor.

Article Snippet: Enzyme linked immune-sorbant assays (ELISA) The effect of IL-1b and/or EGCG (100 μM) on the level of IL-6, IL-8 and TNF-a secreted by chondrocytes in the culture medium was further confirmed by ELISAs (R&D Systems, Minneapolis, MN, USA) and all the assays were performed according to the manufacturer’s instructions.

Techniques: Gene Expression, Quantitative RT-PCR, Sandwich ELISA, Control

Journal: Arthritis research & therapy

Article Title: Epigallocatechin-3-gallate suppresses the global interleukin-1beta-induced inflammatory response in human chondrocytes.

doi: 10.1186/ar3368

Figure Lengend Snippet: Figure 5 EGCG mediated inhibition of NF-B and MAPKs in IL- 1b stimulated human OA chondrocytes and regulation of IL-6, IL-8 and TNF-a. (a) Human OA chondrocytes were pretreated with EGCG (10 to 100 μM) for 2 h and stimulated with IL-1b for 24 h. NF- Bp65 was determined in cell lysate by highly sensitive and specific ELISA (Assay design). TNF-a-treated extract of HeLa cells (supplied with the kit) was used as a positive control. The assay is developed with a chemiluminescent substrate and the signal is detected using luminometer (Lumat LB 9507; Berthold Technologies). NF-B p65 activity was expressed as relative light unit (RLU). (b) After pretreatment with EGCG (10 to 100 μM) for one hour, chondrocytes were stimulated with IL-1b for one half-hour, and then phosphorylation of JNK, ERK and p38-MAPK was determined by Western immunobloting. (c, d, and e) Effect of specific inhibitors for mitogen activated protein kinases and NF-B on the gene expression of IL-6, IL-8 and TNF-a in IL-1b stimulated human OA chondrocytes. Primary chondrocytes were pretreated with specific inhibitors for 2 h and were stimulated with IL-1b for 6 h. Relative gene expression of IL-6, IL-8 and TNF-a normalized to GAPDH and compared with un-stimulated control, were determined by quantitative RT-PCR. Concentrations of specific inhibitors of p38 (SB202190), JNK (SP600125), ERK (PD98059) and NF-B (MG132) used in these studies were 100 μM, 10 μM, 50 μM and 100 μM, respectively. Value represents Mean ± SE of three different patients. # represents P < 0.05 Vs control; * P < 0.05 Vs IL-1b stimulated chondrocytes. EGCG, epigallocatechin-3-gallate;IL-interleukin; ERK, extracellular signal-regulated kinases; JNK, cJun-N-termial Kinases; MAPKs, mitogen activated protein kinases; NF-B, nuclear factor kappa-B; TNF, tumor necrosis factor.

Article Snippet: Enzyme linked immune-sorbant assays (ELISA) The effect of IL-1b and/or EGCG (100 μM) on the level of IL-6, IL-8 and TNF-a secreted by chondrocytes in the culture medium was further confirmed by ELISAs (R&D Systems, Minneapolis, MN, USA) and all the assays were performed according to the manufacturer’s instructions.

Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Positive Control, Activity Assay, Phospho-proteomics, Western Blot, Gene Expression, Control, Quantitative RT-PCR

Journal: Arthritis research & therapy

Article Title: Epigallocatechin-3-gallate suppresses the global interleukin-1beta-induced inflammatory response in human chondrocytes.

doi: 10.1186/ar3368

Figure Lengend Snippet: Figure 6 Gene expression and production of TRAF-6 and IRAK-1 in IL-1b-stimulated human OA chondrocytes. (a) and (c) Effect of EGCG pretreatment on the gene expression of TRAF-6 and IRAK-1 in IL-1b-stimulated OA chondrocytes, respectively. (b) and (d) Effect of EGCG on the production of TRAF-6 and IRAK-1 in IL-1b-stimulated OA chondrocytes, respectively. Value represents Mean ± SD of three different patients. # represents P < 0.05 Vs control; * P < 0.05 Vs IL-1b stimulated chondrocytes. EGCG, epigallocatechin-3-gallate; IL, interleukin; IRAK-1, interleukin-1 receptor-associated kinase 1; TRAF-6, TNF receptor-associated factor 6.

Article Snippet: Enzyme linked immune-sorbant assays (ELISA) The effect of IL-1b and/or EGCG (100 μM) on the level of IL-6, IL-8 and TNF-a secreted by chondrocytes in the culture medium was further confirmed by ELISAs (R&D Systems, Minneapolis, MN, USA) and all the assays were performed according to the manufacturer’s instructions.

Techniques: Gene Expression, Control

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Nanchangmycin suppresses influenza A virus infection by blocking endosomal acidification

doi: 10.3724/abbs.2025102

Figure Lengend Snippet: Subcellular distribution of viral NP MDCK cells were infected with H1N1 at an MOI of 2 for 4 h at 37°C in the presence of DMSO, nanchangmycin, EGCG, or RBV. The viral NP protein was stained with an NP-specific monoclonal antibody and an Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (green). The nuclei were counterstained with DAPI (blue). Scale bar, 50 μm.

Article Snippet: Oseltamivir carboxylate (OSV), oseltamivir phosphate (OSE), epigallocatechin gallate (EGCG) and ribavirin (RBV) were purchased from TargetMol.

Techniques: Infection, Staining

Journal: Journal of agricultural and food chemistry

Article Title: Quantitation of Flavanols, Proanthocyanidins, Isoflavones, Flavanones, Dihydrochalcones, Stilbenes, Benzoic Acid Derivatives Using Ultraviolet Absorbance after Identification by Liquid Chromatography-Mass Spectrometry

doi: 10.1021/jf3006905

Figure Lengend Snippet: Catechins and Proanthocyanidins at 278 nm a

Article Snippet: Theaflavin, theaflavin 3- and 3′- O -gallate mixture, procyanidin B 1 , procyanidin B 2 , procyanidin C 1 , (+)-catechin, (−)-epicatechin, (−)-epicatechin-3- O -gallate, (−)-gallocatechin, (−)-epigallocatechin, (−)-epigallocatechin-3- O -gallate, (+)-taxifolin, daidzin, genistein, genistin, and glycetin were obtained from Chromadex, Inc. (Irvine, CA).

Techniques: