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  • 99
    Qiagen effectene
    BTK expression is induced in HIV-1 infected cells (A) To assess BTK expression in a series of HIV-1 matched cell targets, 50 µg of whole cell extracts from B cells (BJAB), uninfected T cells (CEM and Jurkat), uninfected monocytic cells (U937 and U937-derived macrophage model cells, mΦ), HIV-1 infected T cells (ACH2 and J1.1), and infected monocytic cells (U1 and U1 mΦ) were run on a 4–20% SDS-PAGE and immunoblotted against BTK and β-actin. (B) Jurkat and U937 cells were transfected with the HIV-1 clone pNL4-3 using <t>Effectene</t> transfection reagent, harvested at 48 hr and lysed in Laemmli sample buffer. Twenty microgram of whole cell extract was assayed by western blot using α-BTK and α-β-actin antibodies. Phosphorylated BTK (p-BTK) is suggestive of BTK activation. (C) Cytosolic and nuclear extracts were prepared from Jurkat and J1.1 cells as described in Materials and Methods. The extracts were analyzed by western blot using anti-BTK antibody. Lamin A was used as a nuclear control. (D) Total cell lysates of both uninfected U937 and infected U1 cells were loaded onto a size-exclusion chromatography column and proteins separated in the presence of high salt buffer (500 mM NaCl) to minimize non-specific binding. No detergent was used during fractionation. A sampling (250 µl) of every 5 th fraction from 10 – 55 was acetone precipitated and resuspended in 30 µl of Laemmli buffer, and then 15 µl were run on a gel for western blotting using BTK and β-actin antibody. More soluble actin was present in the U1 fractions as compared to uninfected U937 cells.
    Effectene, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 14020 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/effectene/product/Qiagen
    Average 99 stars, based on 14020 article reviews
    Price from $9.99 to $1999.99
    effectene - by Bioz Stars, 2020-05
    99/100 stars
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    92
    Thermo Fisher effectene
    BTK expression is induced in HIV-1 infected cells (A) To assess BTK expression in a series of HIV-1 matched cell targets, 50 µg of whole cell extracts from B cells (BJAB), uninfected T cells (CEM and Jurkat), uninfected monocytic cells (U937 and U937-derived macrophage model cells, mΦ), HIV-1 infected T cells (ACH2 and J1.1), and infected monocytic cells (U1 and U1 mΦ) were run on a 4–20% SDS-PAGE and immunoblotted against BTK and β-actin. (B) Jurkat and U937 cells were transfected with the HIV-1 clone pNL4-3 using <t>Effectene</t> transfection reagent, harvested at 48 hr and lysed in Laemmli sample buffer. Twenty microgram of whole cell extract was assayed by western blot using α-BTK and α-β-actin antibodies. Phosphorylated BTK (p-BTK) is suggestive of BTK activation. (C) Cytosolic and nuclear extracts were prepared from Jurkat and J1.1 cells as described in Materials and Methods. The extracts were analyzed by western blot using anti-BTK antibody. Lamin A was used as a nuclear control. (D) Total cell lysates of both uninfected U937 and infected U1 cells were loaded onto a size-exclusion chromatography column and proteins separated in the presence of high salt buffer (500 mM NaCl) to minimize non-specific binding. No detergent was used during fractionation. A sampling (250 µl) of every 5 th fraction from 10 – 55 was acetone precipitated and resuspended in 30 µl of Laemmli buffer, and then 15 µl were run on a gel for western blotting using BTK and β-actin antibody. More soluble actin was present in the U1 fractions as compared to uninfected U937 cells.
    Effectene, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/effectene/product/Thermo Fisher
    Average 92 stars, based on 178 article reviews
    Price from $9.99 to $1999.99
    effectene - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    91
    Promega effectene
    BTK expression is induced in HIV-1 infected cells (A) To assess BTK expression in a series of HIV-1 matched cell targets, 50 µg of whole cell extracts from B cells (BJAB), uninfected T cells (CEM and Jurkat), uninfected monocytic cells (U937 and U937-derived macrophage model cells, mΦ), HIV-1 infected T cells (ACH2 and J1.1), and infected monocytic cells (U1 and U1 mΦ) were run on a 4–20% SDS-PAGE and immunoblotted against BTK and β-actin. (B) Jurkat and U937 cells were transfected with the HIV-1 clone pNL4-3 using <t>Effectene</t> transfection reagent, harvested at 48 hr and lysed in Laemmli sample buffer. Twenty microgram of whole cell extract was assayed by western blot using α-BTK and α-β-actin antibodies. Phosphorylated BTK (p-BTK) is suggestive of BTK activation. (C) Cytosolic and nuclear extracts were prepared from Jurkat and J1.1 cells as described in Materials and Methods. The extracts were analyzed by western blot using anti-BTK antibody. Lamin A was used as a nuclear control. (D) Total cell lysates of both uninfected U937 and infected U1 cells were loaded onto a size-exclusion chromatography column and proteins separated in the presence of high salt buffer (500 mM NaCl) to minimize non-specific binding. No detergent was used during fractionation. A sampling (250 µl) of every 5 th fraction from 10 – 55 was acetone precipitated and resuspended in 30 µl of Laemmli buffer, and then 15 µl were run on a gel for western blotting using BTK and β-actin antibody. More soluble actin was present in the U1 fractions as compared to uninfected U937 cells.
    Effectene, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/effectene/product/Promega
    Average 91 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    effectene - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    92
    Roche effectene
    BTK expression is induced in HIV-1 infected cells (A) To assess BTK expression in a series of HIV-1 matched cell targets, 50 µg of whole cell extracts from B cells (BJAB), uninfected T cells (CEM and Jurkat), uninfected monocytic cells (U937 and U937-derived macrophage model cells, mΦ), HIV-1 infected T cells (ACH2 and J1.1), and infected monocytic cells (U1 and U1 mΦ) were run on a 4–20% SDS-PAGE and immunoblotted against BTK and β-actin. (B) Jurkat and U937 cells were transfected with the HIV-1 clone pNL4-3 using <t>Effectene</t> transfection reagent, harvested at 48 hr and lysed in Laemmli sample buffer. Twenty microgram of whole cell extract was assayed by western blot using α-BTK and α-β-actin antibodies. Phosphorylated BTK (p-BTK) is suggestive of BTK activation. (C) Cytosolic and nuclear extracts were prepared from Jurkat and J1.1 cells as described in Materials and Methods. The extracts were analyzed by western blot using anti-BTK antibody. Lamin A was used as a nuclear control. (D) Total cell lysates of both uninfected U937 and infected U1 cells were loaded onto a size-exclusion chromatography column and proteins separated in the presence of high salt buffer (500 mM NaCl) to minimize non-specific binding. No detergent was used during fractionation. A sampling (250 µl) of every 5 th fraction from 10 – 55 was acetone precipitated and resuspended in 30 µl of Laemmli buffer, and then 15 µl were run on a gel for western blotting using BTK and β-actin antibody. More soluble actin was present in the U1 fractions as compared to uninfected U937 cells.
    Effectene, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/effectene/product/Roche
    Average 92 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    effectene - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Stratagene effectene
    BTK expression is induced in HIV-1 infected cells (A) To assess BTK expression in a series of HIV-1 matched cell targets, 50 µg of whole cell extracts from B cells (BJAB), uninfected T cells (CEM and Jurkat), uninfected monocytic cells (U937 and U937-derived macrophage model cells, mΦ), HIV-1 infected T cells (ACH2 and J1.1), and infected monocytic cells (U1 and U1 mΦ) were run on a 4–20% SDS-PAGE and immunoblotted against BTK and β-actin. (B) Jurkat and U937 cells were transfected with the HIV-1 clone pNL4-3 using <t>Effectene</t> transfection reagent, harvested at 48 hr and lysed in Laemmli sample buffer. Twenty microgram of whole cell extract was assayed by western blot using α-BTK and α-β-actin antibodies. Phosphorylated BTK (p-BTK) is suggestive of BTK activation. (C) Cytosolic and nuclear extracts were prepared from Jurkat and J1.1 cells as described in Materials and Methods. The extracts were analyzed by western blot using anti-BTK antibody. Lamin A was used as a nuclear control. (D) Total cell lysates of both uninfected U937 and infected U1 cells were loaded onto a size-exclusion chromatography column and proteins separated in the presence of high salt buffer (500 mM NaCl) to minimize non-specific binding. No detergent was used during fractionation. A sampling (250 µl) of every 5 th fraction from 10 – 55 was acetone precipitated and resuspended in 30 µl of Laemmli buffer, and then 15 µl were run on a gel for western blotting using BTK and β-actin antibody. More soluble actin was present in the U1 fractions as compared to uninfected U937 cells.
    Effectene, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/effectene/product/Stratagene
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    effectene - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    BTK expression is induced in HIV-1 infected cells (A) To assess BTK expression in a series of HIV-1 matched cell targets, 50 µg of whole cell extracts from B cells (BJAB), uninfected T cells (CEM and Jurkat), uninfected monocytic cells (U937 and U937-derived macrophage model cells, mΦ), HIV-1 infected T cells (ACH2 and J1.1), and infected monocytic cells (U1 and U1 mΦ) were run on a 4–20% SDS-PAGE and immunoblotted against BTK and β-actin. (B) Jurkat and U937 cells were transfected with the HIV-1 clone pNL4-3 using Effectene transfection reagent, harvested at 48 hr and lysed in Laemmli sample buffer. Twenty microgram of whole cell extract was assayed by western blot using α-BTK and α-β-actin antibodies. Phosphorylated BTK (p-BTK) is suggestive of BTK activation. (C) Cytosolic and nuclear extracts were prepared from Jurkat and J1.1 cells as described in Materials and Methods. The extracts were analyzed by western blot using anti-BTK antibody. Lamin A was used as a nuclear control. (D) Total cell lysates of both uninfected U937 and infected U1 cells were loaded onto a size-exclusion chromatography column and proteins separated in the presence of high salt buffer (500 mM NaCl) to minimize non-specific binding. No detergent was used during fractionation. A sampling (250 µl) of every 5 th fraction from 10 – 55 was acetone precipitated and resuspended in 30 µl of Laemmli buffer, and then 15 µl were run on a gel for western blotting using BTK and β-actin antibody. More soluble actin was present in the U1 fractions as compared to uninfected U937 cells.

    Journal: Journal of neurovirology

    Article Title: Role of Bruton’s Tyrosine Kinase inhibitors in HIV-1 infected cells

    doi: 10.1007/s13365-015-0323-5

    Figure Lengend Snippet: BTK expression is induced in HIV-1 infected cells (A) To assess BTK expression in a series of HIV-1 matched cell targets, 50 µg of whole cell extracts from B cells (BJAB), uninfected T cells (CEM and Jurkat), uninfected monocytic cells (U937 and U937-derived macrophage model cells, mΦ), HIV-1 infected T cells (ACH2 and J1.1), and infected monocytic cells (U1 and U1 mΦ) were run on a 4–20% SDS-PAGE and immunoblotted against BTK and β-actin. (B) Jurkat and U937 cells were transfected with the HIV-1 clone pNL4-3 using Effectene transfection reagent, harvested at 48 hr and lysed in Laemmli sample buffer. Twenty microgram of whole cell extract was assayed by western blot using α-BTK and α-β-actin antibodies. Phosphorylated BTK (p-BTK) is suggestive of BTK activation. (C) Cytosolic and nuclear extracts were prepared from Jurkat and J1.1 cells as described in Materials and Methods. The extracts were analyzed by western blot using anti-BTK antibody. Lamin A was used as a nuclear control. (D) Total cell lysates of both uninfected U937 and infected U1 cells were loaded onto a size-exclusion chromatography column and proteins separated in the presence of high salt buffer (500 mM NaCl) to minimize non-specific binding. No detergent was used during fractionation. A sampling (250 µl) of every 5 th fraction from 10 – 55 was acetone precipitated and resuspended in 30 µl of Laemmli buffer, and then 15 µl were run on a gel for western blotting using BTK and β-actin antibody. More soluble actin was present in the U1 fractions as compared to uninfected U937 cells.

    Article Snippet: HIV-1 proviral DNA construct pNL4.3 (5 µg) was also transfected in U937 and Jurkat cells using Effectene (Qiagen) according to the manufacturer's instructions ( ).

    Techniques: Expressing, Infection, Derivative Assay, SDS Page, Transfection, Western Blot, Activation Assay, Size-exclusion Chromatography, Binding Assay, Fractionation, Sampling

    siRNA mediated BTK knockdown induces apoptosis in HIV-1 infected cells (A) Latently infected HLM-1 cells were used for selective depletion with siLuc (control) or two different siRNAs targeting BTK (siBTK #1 and siBTK #2). Log phase growing cells (5×10 5 /ml) were transfected with either siLuc or siBTK (10 nm) using Effectene. Cells were harvested at 24, 48 and 72 h post-transfection, and 20 µg of whole cell lysates were resolved by SDS-PAGE and immunoblotted against BTK to assay for effective siRNA-mediated BTK downregulation. (B) Similar to panel A, where cell lysates were further probed with α-caspase 3 and α-PARP antibodies as markers of program cell death. Western blots against β-actin were performed as a loading control. (C) Similar to panel A, where HLM-1 cells were transfected with siLuc, siBTK #1 and siBTK #2 (50 nM). Viability was assessed using CellTiter-Glo assay 72 hours post-treatment. Results are shown as mean of three independent experiments ± SD.

    Journal: Journal of neurovirology

    Article Title: Role of Bruton’s Tyrosine Kinase inhibitors in HIV-1 infected cells

    doi: 10.1007/s13365-015-0323-5

    Figure Lengend Snippet: siRNA mediated BTK knockdown induces apoptosis in HIV-1 infected cells (A) Latently infected HLM-1 cells were used for selective depletion with siLuc (control) or two different siRNAs targeting BTK (siBTK #1 and siBTK #2). Log phase growing cells (5×10 5 /ml) were transfected with either siLuc or siBTK (10 nm) using Effectene. Cells were harvested at 24, 48 and 72 h post-transfection, and 20 µg of whole cell lysates were resolved by SDS-PAGE and immunoblotted against BTK to assay for effective siRNA-mediated BTK downregulation. (B) Similar to panel A, where cell lysates were further probed with α-caspase 3 and α-PARP antibodies as markers of program cell death. Western blots against β-actin were performed as a loading control. (C) Similar to panel A, where HLM-1 cells were transfected with siLuc, siBTK #1 and siBTK #2 (50 nM). Viability was assessed using CellTiter-Glo assay 72 hours post-treatment. Results are shown as mean of three independent experiments ± SD.

    Article Snippet: HIV-1 proviral DNA construct pNL4.3 (5 µg) was also transfected in U937 and Jurkat cells using Effectene (Qiagen) according to the manufacturer's instructions ( ).

    Techniques: Infection, Transfection, SDS Page, Western Blot, Glo Assay

    Activation of NF-κB by P. aeruginosa is dependent on flagellin protein. HAECs were prepared and transfected in 24-well plates as described in Materials and Methods. Using the Effectene reagent, plasmids containing 0.3 μg of the hBD2 promoter-driven

    Journal:

    Article Title: Human Airway Epithelial Cells Sense Pseudomonas aeruginosa Infection via Recognition of Flagellin by Toll-Like Receptor 5

    doi: 10.1128/IAI.73.11.7151-7160.2005

    Figure Lengend Snippet: Activation of NF-κB by P. aeruginosa is dependent on flagellin protein. HAECs were prepared and transfected in 24-well plates as described in Materials and Methods. Using the Effectene reagent, plasmids containing 0.3 μg of the hBD2 promoter-driven

    Article Snippet: Efficient transfection of the primary HAECs was obtained by using the Effectene reagent.

    Techniques: Activation Assay, Transfection

    PACA as a covalent activator for PPAR-γ. ( A ) Docking of OXO-PACA to PPAR-γ LBD. OXO-PACA was docked to PPAR-γ LBD (PDB:) by Autodock vina. ( B ) Amino acid residues for recognizing OXO-PACA. Hydrogen bonds are shown in green, whereas pi-alkyl interactions are shown in pink. ( C ) Luciferase assay for PPAR-γ activation. RAW264.7 macrophages were transiently transfected with PPRE-X3-TK luciferase and pRL control using Effectene transfection reagent from Qiagen. Transfected macrophages were treated with PACA for 24 h. Luciferase activities were measured using the Dual-Luciferase reporter assay system. Data were expressed as mean ± SD (n = 5). * p

    Journal: Aging (Albany NY)

    Article Title: N-Propargyl caffeate amide (PACA) prevents cardiac fibrosis in experimental myocardial infarction by promoting pro-resolving macrophage polarization

    doi: 10.18632/aging.102959

    Figure Lengend Snippet: PACA as a covalent activator for PPAR-γ. ( A ) Docking of OXO-PACA to PPAR-γ LBD. OXO-PACA was docked to PPAR-γ LBD (PDB:) by Autodock vina. ( B ) Amino acid residues for recognizing OXO-PACA. Hydrogen bonds are shown in green, whereas pi-alkyl interactions are shown in pink. ( C ) Luciferase assay for PPAR-γ activation. RAW264.7 macrophages were transiently transfected with PPRE-X3-TK luciferase and pRL control using Effectene transfection reagent from Qiagen. Transfected macrophages were treated with PACA for 24 h. Luciferase activities were measured using the Dual-Luciferase reporter assay system. Data were expressed as mean ± SD (n = 5). * p

    Article Snippet: Luciferase reporter assays RAW264.7 macrophages in 12-well plated were co-transfected with 300 ng of PPRE-X3-TK controlled Firefly luciferase plasmid from Addgene (Cambridge, MA, USA) and 30 ng of pRL-TK-renilla luciferase plasmid using Effectene transfection reagent from Qiagen (Hilden, Germany).

    Techniques: Luciferase, Activation Assay, Transfection, Reporter Assay