Journal: Langenbeck's Archives of Surgery
Article Title: A fast intraoperative PTH point-of-care assay on the Philips handheld magnotech system
Figure Lengend Snippet: Fifty-percent drop of PTH levels 10 min after resection of the parathyroid adenoma. Various EDTA-plasma samples were collected during two surgeries of parathyroid adenomas; samples before surgery ( M1 ), just before resection ( M2 ), 5 min ( M3 ), 10 min ( M4 ), and 20 min ( M5 ) after resection. These samples were measured with the STAT-intraoperative intact-PTH assay on site ( a and c ) and, after a freeze-thaw step, the signal (arbitrary units) induced by these samples were measured in cartridges that contained a detection spot with anti-C-terminal PTH antibodies and anti-N-terminal PTH antibody-coated beads, using 0.4 μL detection volume and an 8-min assay protocol. The PTH levels in sample M2 were used to normalize the signal obtained with the magnotech ioPTH assay to 100% ( b and d )
Article Snippet: To prove the linear correlation between PTH concentrations covering the clinical relevant range ( > 70 pg/mL), and the signals obtained with the magnotech ioPTH assay, we used 20 EDTA-plasma samples with PTH levels measured with a commercially available reference PTH assay (Intact PTH, Roche Modular Analytics E170, Roche Diagnostics, Germany).