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Enzo Biochem
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ViraQuest Inc
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Microsynth ag
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Abnova
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Image Search Results
Journal: PLoS ONE
Article Title: Taurine Supplementation Reduces Blood Pressure and Prevents Endothelial Dysfunction and Oxidative Stress in Post-Weaning Protein-Restricted Rats
doi: 10.1371/journal.pone.0105851
Figure Lengend Snippet: Protein expression of gp91 phox (A), p47 phox (B), EC-SOD (C), CuZn-SOD (D), Mn-SOD (E), catalase (F), glutathione peroxidase (GPx) (G), eNOS (H) and phosphorylated Ser1177 (p)eNOS (I) were analyzed by Western-blot in aorta from rats fed normal-protein (NP) or low-protein (LP), with or without taurine (T). α-actin content was used as internal control in each sample. Values represent the mean ± SEM (Number of animals in each group = 5–9). 2-way ANOVA:Figure 4B and 4C showed significant interaction (LP diet x taurine).Figure 4E showed main effect of LP diet;Figure 4I showed significant main effects of LP diet and taurine. Bonferroni post-test (p<0.05): * vs . NP, + vs . LP, # vs . NPT.
Article Snippet: Primary antibodies: anti-eNOS from BD Transduction Laboratories (Franklin Lakes, NJ, USA); anti-phospho-eNOS (Ser 1177) and anti-CuZn-SOD from Cell Signaling Technology (Beverly, MA, USA);
Techniques: Expressing, Western Blot
Journal: Pulmonary Circulation
Article Title: Hydrocortisone normalizes phosphodiesterase-5 activity in pulmonary artery smooth muscle cells from lambs with persistent pulmonary hypertension of the newborn
doi: 10.1086/674903
Figure Lengend Snippet: Hydrocortisone (HC) increases expression of extracellular superoxide dismutase (ecSOD) in fetal pulmonary artery smooth muscle cells (FPASMCs) from lambs with persistent pulmonary hypertension of the newborn (PPHN). A, PPHN FPASMC were exposed to 21% O2–5% CO2 or 95% O2–5% CO2 ± 100 nM HC for 24 hours. Cells were harvested for total protein and subjected to ecSOD Western blot analysis, with β-actin normalization. Data are shown as means ± SEM (n = 7 for 21% untreated, 21% plus 100 nM HC, 95% untreated, n = 8 for 95% plus 100 nM HC). Number sign indicates P < 0.05 versus 95% untreated. B, Representative Western blots are shown for ecSOD and β-actin in PPHN FPASMC.
Article Snippet: 13 Membranes were blocked for 1 hour at room temperature with 5% nonfat dry milk in Tris-buffered saline containing 0.1% Tween 20 (1X TBST) and incubated overnight at 4°C with primary antibody in 5% milk plus 1X TBST at an appropriate dilution (1:500 for mouse anti-PDE5 [BD Transduction], 1∶500 for goat anti-IκB [Santa Cruz Biotechnology], 1∶1,000 for rabbit anti-Nox1 [Santa Cruz Biotechnology], 1∶1000 for rabbit anti-Nox4 [Santa Cruz Biotechnology], 1∶1,000 for
Techniques: Expressing, Western Blot
Journal: Pulmonary Circulation
Article Title: Hydrocortisone normalizes phosphodiesterase-5 activity in pulmonary artery smooth muscle cells from lambs with persistent pulmonary hypertension of the newborn
doi: 10.1086/674903
Figure Lengend Snippet: Model for the effects of hydrocortisone on phosphodiesterase-5 (PDE5) in fetal pulmonary artery smooth muscle cells (FPASMCs) from lambs with persistent pulmonary hypertension of the newborn (PPHN). Exposure to hyperoxia leads to increased reactive oxygen species (ROS) production, resulting in increased nuclear factor κ B (NFκB) activity with subsequent induction of PDE5 activity. Hydrocortisone disrupts this pathway by increasing extracellular superoxide dismutase (ecSOD) expression, leading to attenuation of ROS signaling and normalization of PDE5 activity. IκB: NFκB inhibitory protein.
Article Snippet: 13 Membranes were blocked for 1 hour at room temperature with 5% nonfat dry milk in Tris-buffered saline containing 0.1% Tween 20 (1X TBST) and incubated overnight at 4°C with primary antibody in 5% milk plus 1X TBST at an appropriate dilution (1:500 for mouse anti-PDE5 [BD Transduction], 1∶500 for goat anti-IκB [Santa Cruz Biotechnology], 1∶1,000 for rabbit anti-Nox1 [Santa Cruz Biotechnology], 1∶1000 for rabbit anti-Nox4 [Santa Cruz Biotechnology], 1∶1,000 for
Techniques: Activity Assay, Expressing