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  • 91
    GE Healthcare enhanced chemiluminescence ecl
    Proteins in extracts from broken and fractionated spores were separated on an SDS–12% polyacrylamide gel and transferred to <t>PVDF</t> membrane for Western blotting by the Amersham <t>ECL</t> chemiluminescence detection system. Each sample track contains ca. 11 μg of protein. Detection of GerAC was achieved using anti-GerAC antibody, diluted at 1/500. The secondary antibody was used at 1/15,000 dilution. Lane 1 contained biotinylated protein standards (Sigma SDS-6B: sizes of 205, 116, 97, 58, 40, 29, 20, 14.3, and 6.5 kDa). Arrows by the marker lane indicate the 58 and 40-kDa markers, and the arrow on the right of the gel indicates the GerAC band. Lane 2 contained prestained protein markers, lane 3 contained total extract from broken spores of strain 1604, and lanes 4 to 6 contained integument, membrane, and soluble fractions, respectively, of strain 1604. Lanes 7 and 8 contained total spore extracts of strains AM418 ( gerA Δ) and AM1422 ( gerA Δ gerB null), respectively.
    Enhanced Chemiluminescence Ecl, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 547 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co enhanced chemiluminescence ecl
    Proteins in extracts from broken and fractionated spores were separated on an SDS–12% polyacrylamide gel and transferred to <t>PVDF</t> membrane for Western blotting by the Amersham <t>ECL</t> chemiluminescence detection system. Each sample track contains ca. 11 μg of protein. Detection of GerAC was achieved using anti-GerAC antibody, diluted at 1/500. The secondary antibody was used at 1/15,000 dilution. Lane 1 contained biotinylated protein standards (Sigma SDS-6B: sizes of 205, 116, 97, 58, 40, 29, 20, 14.3, and 6.5 kDa). Arrows by the marker lane indicate the 58 and 40-kDa markers, and the arrow on the right of the gel indicates the GerAC band. Lane 2 contained prestained protein markers, lane 3 contained total extract from broken spores of strain 1604, and lanes 4 to 6 contained integument, membrane, and soluble fractions, respectively, of strain 1604. Lanes 7 and 8 contained total spore extracts of strains AM418 ( gerA Δ) and AM1422 ( gerA Δ gerB null), respectively.
    Enhanced Chemiluminescence Ecl, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Beyotime ecl enhanced chemiluminescence kit
    Proteins in extracts from broken and fractionated spores were separated on an SDS–12% polyacrylamide gel and transferred to <t>PVDF</t> membrane for Western blotting by the Amersham <t>ECL</t> chemiluminescence detection system. Each sample track contains ca. 11 μg of protein. Detection of GerAC was achieved using anti-GerAC antibody, diluted at 1/500. The secondary antibody was used at 1/15,000 dilution. Lane 1 contained biotinylated protein standards (Sigma SDS-6B: sizes of 205, 116, 97, 58, 40, 29, 20, 14.3, and 6.5 kDa). Arrows by the marker lane indicate the 58 and 40-kDa markers, and the arrow on the right of the gel indicates the GerAC band. Lane 2 contained prestained protein markers, lane 3 contained total extract from broken spores of strain 1604, and lanes 4 to 6 contained integument, membrane, and soluble fractions, respectively, of strain 1604. Lanes 7 and 8 contained total spore extracts of strains AM418 ( gerA Δ) and AM1422 ( gerA Δ gerB null), respectively.
    Ecl Enhanced Chemiluminescence Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare ecl enhanced chemiluminescence kit
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Ecl Enhanced Chemiluminescence Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Denville Scientific hyglo enhanced chemiluminescence ecl
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Hyglo Enhanced Chemiluminescence Ecl, supplied by Denville Scientific, used in various techniques. Bioz Stars score: 90/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare hyperfilm enhanced chemiluminescence ecl
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Hyperfilm Enhanced Chemiluminescence Ecl, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    GE Healthcare ecl enhanced chemiluminescence reagent
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Ecl Enhanced Chemiluminescence Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EuroClone enhanced chemiluminescence ecl system
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Enhanced Chemiluminescence Ecl System, supplied by EuroClone, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer ecl enhanced chemiluminescence kit
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Ecl Enhanced Chemiluminescence Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Lumigen ecl enhanced chemiluminescence substrate
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Ecl Enhanced Chemiluminescence Substrate, supplied by Lumigen, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ecl enhanced chemiluminescence substrate
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Ecl Enhanced Chemiluminescence Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore enhanced chemiluminescence ecl
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Enhanced Chemiluminescence Ecl, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad ecl enhanced chemiluminescence agent
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Ecl Enhanced Chemiluminescence Agent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher enhanced chemiluminescence ecl kit
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Enhanced Chemiluminescence Ecl Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer luminol enhanced chemiluminescence ecl
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Luminol Enhanced Chemiluminescence Ecl, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa ecl enhanced chemiluminescence kit
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Ecl Enhanced Chemiluminescence Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boster Bio ecl enhanced chemiluminescence
    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using <t>HRP-conjugated</t> secondary antibodies and an enhanced chemiluminescence kit.
    Ecl Enhanced Chemiluminescence, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Proteins in extracts from broken and fractionated spores were separated on an SDS–12% polyacrylamide gel and transferred to PVDF membrane for Western blotting by the Amersham ECL chemiluminescence detection system. Each sample track contains ca. 11 μg of protein. Detection of GerAC was achieved using anti-GerAC antibody, diluted at 1/500. The secondary antibody was used at 1/15,000 dilution. Lane 1 contained biotinylated protein standards (Sigma SDS-6B: sizes of 205, 116, 97, 58, 40, 29, 20, 14.3, and 6.5 kDa). Arrows by the marker lane indicate the 58 and 40-kDa markers, and the arrow on the right of the gel indicates the GerAC band. Lane 2 contained prestained protein markers, lane 3 contained total extract from broken spores of strain 1604, and lanes 4 to 6 contained integument, membrane, and soluble fractions, respectively, of strain 1604. Lanes 7 and 8 contained total spore extracts of strains AM418 ( gerA Δ) and AM1422 ( gerA Δ gerB null), respectively.

    Journal: Journal of Bacteriology

    Article Title: Localization of GerAA and GerAC Germination Proteins in the Bacillus subtilis Spore

    doi: 10.1128/JB.183.14.4317-4322.2001

    Figure Lengend Snippet: Proteins in extracts from broken and fractionated spores were separated on an SDS–12% polyacrylamide gel and transferred to PVDF membrane for Western blotting by the Amersham ECL chemiluminescence detection system. Each sample track contains ca. 11 μg of protein. Detection of GerAC was achieved using anti-GerAC antibody, diluted at 1/500. The secondary antibody was used at 1/15,000 dilution. Lane 1 contained biotinylated protein standards (Sigma SDS-6B: sizes of 205, 116, 97, 58, 40, 29, 20, 14.3, and 6.5 kDa). Arrows by the marker lane indicate the 58 and 40-kDa markers, and the arrow on the right of the gel indicates the GerAC band. Lane 2 contained prestained protein markers, lane 3 contained total extract from broken spores of strain 1604, and lanes 4 to 6 contained integument, membrane, and soluble fractions, respectively, of strain 1604. Lanes 7 and 8 contained total spore extracts of strains AM418 ( gerA Δ) and AM1422 ( gerA Δ gerB null), respectively.

    Article Snippet: Proteins were blotted on polyvinylidene difluoride (PVDF) membrane and detected using the Amersham enhanced chemiluminescence (ECL) procedures, according to the manufacturer's instructions.

    Techniques: Western Blot, Marker

    Coimmunoprecipitation of Ku with transcription proteins. Immunoprecipitation was performed with anti-RNAP IIo (MAb H5), anti-RNAP IIa (MAb 8WG16), anti-Ku80 (MAb S10B1), anti-DSIF (MAb; Pharmingen), or control (mixture of mouse IgG and IgM, unless otherwise indicated) antibody. Antibodies were cross-linked to agarose beads as described in Materials and Methods. Immunoblots were developed with the ECL enhanced chemiluminescence system (Amersham Pharmacia Biotech) unless otherwise indicated. In all panels, input represents 5 to 10% of material used for immunoprecipitation. (A) HCT116 cell immunoprecipitates probed with antibodies to markers of the elongation complex as indicated. Immunoblots were developed with bromochloroindolyl phosphate and nitroblue tetrazolium (left panel) or with the ECL system (right panel). (B) HCT116 cell immunoprecipitates probed with antibodies to markers of the initiation complex. (C) HeLa cell immunoprecipitates probed with markers to the elongation complex. (D) HeLa cell immunoprecipitates probed with markers to the initiation complex. An asterisk by the CDK9 panel indicates a nonspecific background band.

    Journal: Molecular and Cellular Biology

    Article Title: Subnuclear Localization of Ku Protein: Functional Association with RNA Polymerase II Elongation Sites

    doi: 10.1128/MCB.22.22.8088-8099.2002

    Figure Lengend Snippet: Coimmunoprecipitation of Ku with transcription proteins. Immunoprecipitation was performed with anti-RNAP IIo (MAb H5), anti-RNAP IIa (MAb 8WG16), anti-Ku80 (MAb S10B1), anti-DSIF (MAb; Pharmingen), or control (mixture of mouse IgG and IgM, unless otherwise indicated) antibody. Antibodies were cross-linked to agarose beads as described in Materials and Methods. Immunoblots were developed with the ECL enhanced chemiluminescence system (Amersham Pharmacia Biotech) unless otherwise indicated. In all panels, input represents 5 to 10% of material used for immunoprecipitation. (A) HCT116 cell immunoprecipitates probed with antibodies to markers of the elongation complex as indicated. Immunoblots were developed with bromochloroindolyl phosphate and nitroblue tetrazolium (left panel) or with the ECL system (right panel). (B) HCT116 cell immunoprecipitates probed with antibodies to markers of the initiation complex. (C) HeLa cell immunoprecipitates probed with markers to the elongation complex. (D) HeLa cell immunoprecipitates probed with markers to the initiation complex. An asterisk by the CDK9 panel indicates a nonspecific background band.

    Article Snippet: Except as noted, immunoblots were developed with the ECL enhanced chemiluminescence system (Amersham Pharmacia Biotech).

    Techniques: Immunoprecipitation, Western Blot

    Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using HRP-conjugated secondary antibodies and an enhanced chemiluminescence kit.

    Journal: Archaea

    Article Title: Identification of Residues Important for the Activity of Haloferax volcanii AglD, a Component of the Archaeal N-Glycosylation Pathway

    doi: 10.1155/2010/315108

    Figure Lengend Snippet: Expression levels of the various versions of CBD-AglD. Hfx. volcanii cells expressing the various AglD mutants considered in this study were grown to OD 550 1.0 and their protein contents were separated on 10% SDS-PAGE. The CBD-AglD content of each strain was subsequently assessed by immunoblot using polyclonal antiCBD antibodies. Antibody binding was detected using HRP-conjugated secondary antibodies and an enhanced chemiluminescence kit.

    Article Snippet: Antibody binding was detected using goat antirabbit horseradish peroxidase-(HRP-) conjugated antibodies (1 : 4000, BioRad, Hercules, CA) and an ECL-enhanced chemiluminescence kit (Amersham, Buckingham, UK).

    Techniques: Expressing, SDS Page, Binding Assay