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Image Search Results
Journal: Physiological Genomics
Article Title: Proteomics of novel induced pluripotent stem cell-derived vascular endothelial cells reveal extensive similarity with an immortalized human endothelial cell line
doi: 10.1152/physiolgenomics.00166.2022
Figure Lengend Snippet: Efficient and reproducible generation of vascular endothelial cells (iECs) from 5 different iPSC lines. A : schematic of the iEC differentiation protocol. B : protein expression of vascular endothelial markers CD144/CD31 and functional LDL uptake assay of iECs on day 21 using 5 different iPSC lines: CS0007iCTR-n07 (07n07), EDi028-A, C0002iCTR-n11 (02n11), EDi042-A, and CS0003iCTR-n14 (03n14). HUVECs and iPSCs are also included as controls. Scale bar = 150 µm. C : quantification of CD144/CD31 protein expression and LDL uptake dye (% positive cells of DAPI). Data are shown as means ± SE. Immunocytochemistry (ICC) and LDL uptake images shown here are representative results from 18 independent sites acquired, per iPSC cell line. EC, endothelial cell; ECGM, EC growth medium MV2; HUVECs, human umbilical vein endothelial cells; iECs, induced endothelial cells; iPSCs, induced pluripotent stem cells; VEGF, vascular endothelial growth factor.
Article Snippet: The base medium used for phases I and II was STEMdiffAPEL2 (STEMCELL Tech) and for phases III and IV ,
Techniques: Expressing, Functional Assay, Immunocytochemistry
Journal: Cancers
Article Title: Human Adipose-Derived Stem/Stromal Cells Promote Proliferation and Migration in Head and Neck Cancer Cells
doi: 10.3390/cancers13112751
Figure Lengend Snippet: Top left: Branches (yellow lines) in each well were counted using ImageJ Angiogenesis Analyzer software. Top right: Box-whisker plots depicting tube formation (number of branches normalized to MSCM 2 control after subtraction of ECGM with 30 nM Suramin control) of HUVECs cultured with the MSCM 2 control, ECGM (without Suramin) control, or ASC-derived supernatants from five different patients (“ASC (Pat. 1–5)”). Lower half: Representative photographs depicting tube formation in wells corresponding to patients 1–5 (“ASC (Pat. 1–5)”) and controls. p -values ** < 0.01, *** < 0.001.
Article Snippet: Subsequently, cells were resuspended in either the ASC-derived supernatants (cultured with MSCM 2), fresh Endothelial Cell Growth Medium MV (ECGM, PromoCell, Heidelberg, Germany), fresh MSCM 2 (PromoCell, Heidelberg, Germany), or
Techniques: Software, Whisker Assay, Cell Culture, Derivative Assay