Journal: bioRxiv

Article Title: Loss of developmentally derived Irf8+ macrophages promotes hyperinnervation and arrhythmia in the adult zebrafish heart

doi: 10.1101/2024.04.17.589909

Figure Lengend Snippet: (A) Cartoon depiction of the rack-compatible swim tunnel with metered flow. Following a 5-minute acclimation period within the tunnel, 12 mpf zebrafish were subjected to increasing flow rate intervals for a total of 1 hour. (B) Cartoon depiction of an ECG recording and a representative ECG trace from an anesthetized fish placed ventral-side up in a sponge surrounded by anesthesia solution. (C) Representative ECG traces for wild type and irf8 mutant zebrafish following the swim assay. Black arrows indicate unique electrical disturbances occurring above any baseline noise. (D) Percentage of fish with an abnormal electrical event during the ECG recording. Male n = 7 per genotype, ****p < 0.0001. Female n = 4-8 per genotype, ****p < 0.0001. Fisher’s exact test. (E) Categorization of ECG abnormalities observed throughout all wild type and irf8 mutant fish.

Article Snippet: To record electrical impulses, we used the CardioPhys TM ECG system (World Precision Instruments), which included a low-noise gold ECG probe, micromanipulator, bio-amplifier and ground electrode, in combination with the PowerLab 2/26 data acquisition unit (ADInstruments, PL2602).

Techniques: Mutagenesis

Journal: bioRxiv

Article Title: Loss of developmentally derived Irf8+ macrophages promotes hyperinnervation and arrhythmia in the adult zebrafish heart

doi: 10.1101/2024.04.17.589909

Figure Lengend Snippet: (A) Cartoon depiction of an adult zebrafish undergoing an echocardiogram with the transducer in the long-axis (LAX) position. Echocardiograms were performed on unchallenged fish. (B) Cartoon of an adult zebrafish heart from the perspective of the echocardiography recording, denoting the atrium (A), atrioventricular (AV) valve, ventriculobulbar (VB) valve, and bulbus arteriosus (BA). The boxes around the AV valve and VB valve depict the gating strategies to determine inward flow and outward flow of blood from the ventricle. (C) Still image from an adult zebrafish echocardiography recording with the ventricle and bulbus arteriosus outlined in red. (D) Representative echocardiogram trace gating on the AV valve with the early filling (E) wave, late filling (A) wave, diastasis time, isovolumic contraction time (IVCT), aortic ejection time (AET), and isovolumic relaxation time (IVRT) labeled. (E-G) Quantifications of IVRT (E), AET (F), and pressure within the VB valve (G) in male and female wild type and irf8 mutant fish at 6 and 12 mpf. Male n = 10-21 per genotype and timepoint. Female n = 9-14 per genotype and timepoint. Each point represents individual beats per fish. 8-10 beats were analyzed per fish. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (H) Quantification of heart in male and female wild type and irf8 mutants. (I) Representative echocardiogram traces from a wild type and irf8 mutant recording. (J-M) Quantification of diastasis time in wild type males (J) and females (K) at 6 mpf and 12 mpf, as well as diastasis time in irf8 mutant males (L) and females (M) at 6 mpf and 12 mpf. A Gaussian distribution was created by analyzing 8 beats per sample and grouping diastasis duration in defined increments. Male n = 10-21 per genotype and timepoint. Female n = 9-14 per genotype and timepoint. Horizontal bars represent the range of diastasis duration. (N-O) 95% confidence interval of the average diastasis time durations in wild type (gray) and irf8 mutant (blue) males (N) and females (O).

Article Snippet: To record electrical impulses, we used the CardioPhys TM ECG system (World Precision Instruments), which included a low-noise gold ECG probe, micromanipulator, bio-amplifier and ground electrode, in combination with the PowerLab 2/26 data acquisition unit (ADInstruments, PL2602).

Techniques: Labeling, Mutagenesis

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Pretreatment of Tribulus terrestris L. causes anti-ischemic cardioprotection through MAPK mediated anti-apoptotic pathway in rat.

doi: 10.1016/j.biopha.2019.01.033

Figure Lengend Snippet: Fig. 4. Effect of TTM on Electrocardiogram patterns. Electrocardiogram of control rats showed normal ECG patterns, and isoproterenol treatment (85 mg/kg) showed a marked ST-segment elevation. Pretreatment of TTM (250 and 500 mg/kg) prior to isoproterenol treatment showed near normal ECG patterns. There was an increase in heart rate on isoproterenol treatment and TTM (250 and 500 mg/kg) pretreatment prior to isoproterenol treatment was able to normalize the increase in heart rate. Rats were pretreated with TTM 250 and 500 mg/kg body weight and positive control (propranolol) 15 mg/kg body weight for 21 days. Isoproterenol was injected, and after 24 h, rats were anaesthetised using ketamine (65 mg/kg body weight) and xylazine (5 mg/kg body weight) and subjected to ECG analysis.

Article Snippet: ECG recording was performed using a PowerLab data acquisition system and analysed with ECG Analysis Module (AD Instruments, Australia).

Techniques: Control, Positive Control, Injection

Journal: Sensors (Basel, Switzerland)

Article Title: Ambulatory Electrocardiographic Monitoring and Ectopic Beat Detection in Conscious Mice

doi: 10.3390/s20143867

Figure Lengend Snippet: Characteristics of human ambulatory electrocardiography (AECG) and ECG techniques available in mice.

Article Snippet: Next, using a custom-made amplifier (based on TL074 Opamp, Texas Instruments), the ECG signals were high-pass filtered at 0.15 Hz and amplified 512 times.

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Ambulatory Electrocardiographic Monitoring and Ectopic Beat Detection in Conscious Mice

doi: 10.3390/s20143867

Figure Lengend Snippet: Mouse in supine position with schematic representation of the obtained lead configuration (lead I, lead II and lead III) with representative ECG-traces. P-wave, QRS complex, and T-wave are annotated on the ECG-trace in lead I configuration. Vertical and horizontal scale bars represent 1.5 mV and 100 ms, respectively. G; ground, RFP; right front paw, LFP; left front paw, RHP; right hind paw, LHP; left hind paw.

Article Snippet: Next, using a custom-made amplifier (based on TL074 Opamp, Texas Instruments), the ECG signals were high-pass filtered at 0.15 Hz and amplified 512 times.

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Ambulatory Electrocardiographic Monitoring and Ectopic Beat Detection in Conscious Mice

doi: 10.3390/s20143867

Figure Lengend Snippet: Schematic representation of the different pre-processing steps applied to the input file and resulting effect on the ECG trace. FFT; Fast Fourier Transformation.

Article Snippet: Next, using a custom-made amplifier (based on TL074 Opamp, Texas Instruments), the ECG signals were high-pass filtered at 0.15 Hz and amplified 512 times.

Techniques: Transformation Assay

Journal: Sensors (Basel, Switzerland)

Article Title: Ambulatory Electrocardiographic Monitoring and Ectopic Beat Detection in Conscious Mice

doi: 10.3390/s20143867

Figure Lengend Snippet: Sensitivity, precision and specificity of R-peak detection compared between our automated custom algorithms and PhysioZoo ( n = 10 ECG traces of twenty minutes).

Article Snippet: Next, using a custom-made amplifier (based on TL074 Opamp, Texas Instruments), the ECG signals were high-pass filtered at 0.15 Hz and amplified 512 times.

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Ambulatory Electrocardiographic Monitoring and Ectopic Beat Detection in Conscious Mice

doi: 10.3390/s20143867

Figure Lengend Snippet: Graphical user interface presentation of the automatically calculated ECG parameters after running the custom MATLAB ® scripts on a representative twenty-minute murine ECG trace. ( a ) Detected R-peaks are visualized with blue dots on the (compressed) murine ECG trace. Total amount of detected R-peaks and calculated heart rate is presented in the title of the graph. ( b ) Histogram representing the distribution of the observed RR-intervals. X-values are subdivided in intervals of 1 ms. Automated calculation of the mean RR-interval and Full-Width at Half-Maximum (representative for heart rate variability) is presented in the title of the graph. The level of the Full-Width at Half-Maximum value is visualized by means of a red line within the graph. ECG; electrocardiogram, BPM; beats per minute, FWHM; Full-Width at Half-Maximum.

Article Snippet: Next, using a custom-made amplifier (based on TL074 Opamp, Texas Instruments), the ECG signals were high-pass filtered at 0.15 Hz and amplified 512 times.

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Ambulatory Electrocardiographic Monitoring and Ectopic Beat Detection in Conscious Mice

doi: 10.3390/s20143867

Figure Lengend Snippet: Sensitivity, precision and specificity of R-peak detection of the same ECG file at different sample rates analyzed with our custom algorithms.

Article Snippet: Next, using a custom-made amplifier (based on TL074 Opamp, Texas Instruments), the ECG signals were high-pass filtered at 0.15 Hz and amplified 512 times.

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Ambulatory Electrocardiographic Monitoring and Ectopic Beat Detection in Conscious Mice

doi: 10.3390/s20143867

Figure Lengend Snippet: Ectopic beat detection. ( a ) Representative output obtained for ectopic beat detection showing the aberrant RR-interval. Detected R-peaks are indicated with blue dots on the band-pass filtered ECG-trace. ( b ) Correlation analysis between the percentage of bad quality of the ECG trace and false positive and true positive ectopic beat detection ( n = 8 ECG traces of twenty minutes). The number of false positive and true positive ectopic beats are depicted with a grey x and a black +, respectively. ROI; Region of interest.

Article Snippet: Next, using a custom-made amplifier (based on TL074 Opamp, Texas Instruments), the ECG signals were high-pass filtered at 0.15 Hz and amplified 512 times.

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Ambulatory Electrocardiographic Monitoring and Ectopic Beat Detection in Conscious Mice

doi: 10.3390/s20143867

Figure Lengend Snippet: P-wave analysis on representative mouse ECG files at a sample rate of 2000 Hz. ( a ) Blocked P-wave based on the presence of a P-wave (indicated with arrow) without subsequent QRS-complex. ( b ) Sinus arrest based on the absence of an extra P-wave in between a long RR-interval. Vertical and horizontal scale bars represent 0.5 mV and 100 ms, respectively. Hashtag indicates absence of p-wave indicating sinus arrest.

Article Snippet: Next, using a custom-made amplifier (based on TL074 Opamp, Texas Instruments), the ECG signals were high-pass filtered at 0.15 Hz and amplified 512 times.

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Ambulatory Electrocardiographic Monitoring and Ectopic Beat Detection in Conscious Mice

doi: 10.3390/s20143867

Figure Lengend Snippet: Representative ectopic beats recorded in mice using lead I, II and III configurations. ( a ) An ectopic beat with either an atrial or junctional origin, based on a narrow QRS complex; ( b , c ) ectopic beats with origin in the ventricles (based on a broad QRS complex); for ( b ) an inferior axis and for ( c ) a superior axis can be inferred based on the ECG pattern. Vertical and horizontal scale bars represent 1 mV and 100 ms, respectively. Ectopic beats are indicated with asterisks.

Article Snippet: Next, using a custom-made amplifier (based on TL074 Opamp, Texas Instruments), the ECG signals were high-pass filtered at 0.15 Hz and amplified 512 times.

Techniques:

Journal: bioRxiv

Article Title: Loss of developmentally derived Irf8+ macrophages promotes hyperinnervation and arrhythmia in the adult zebrafish heart

doi: 10.1101/2024.04.17.589909

Figure Lengend Snippet: (A) Cartoon depiction of the rack-compatible swim tunnel with metered flow. Following a 5-minute acclimation period within the tunnel, 12 mpf zebrafish were subjected to increasing flow rate intervals for a total of 1 hour. (B) Cartoon depiction of an ECG recording and a representative ECG trace from an anesthetized fish placed ventral-side up in a sponge surrounded by anesthesia solution. (C) Representative ECG traces for wild type and irf8 mutant zebrafish following the swim assay. Black arrows indicate unique electrical disturbances occurring above any baseline noise. (D) Percentage of fish with an abnormal electrical event during the ECG recording. Male n = 7 per genotype, ****p < 0.0001. Female n = 4-8 per genotype, ****p < 0.0001. Fisher’s exact test. (E) Categorization of ECG abnormalities observed throughout all wild type and irf8 mutant fish.

Article Snippet: To record electrical impulses, we used the CardioPhys TM ECG system (World Precision Instruments), which included a low-noise gold ECG probe, micromanipulator, bio-amplifier and ground electrode, in combination with the PowerLab 2/26 data acquisition unit (ADInstruments, PL2602).

Techniques: Mutagenesis

Journal: bioRxiv

Article Title: Loss of developmentally derived Irf8+ macrophages promotes hyperinnervation and arrhythmia in the adult zebrafish heart

doi: 10.1101/2024.04.17.589909

Figure Lengend Snippet: (A) Cartoon depiction of an adult zebrafish undergoing an echocardiogram with the transducer in the long-axis (LAX) position. Echocardiograms were performed on unchallenged fish. (B) Cartoon of an adult zebrafish heart from the perspective of the echocardiography recording, denoting the atrium (A), atrioventricular (AV) valve, ventriculobulbar (VB) valve, and bulbus arteriosus (BA). The boxes around the AV valve and VB valve depict the gating strategies to determine inward flow and outward flow of blood from the ventricle. (C) Still image from an adult zebrafish echocardiography recording with the ventricle and bulbus arteriosus outlined in red. (D) Representative echocardiogram trace gating on the AV valve with the early filling (E) wave, late filling (A) wave, diastasis time, isovolumic contraction time (IVCT), aortic ejection time (AET), and isovolumic relaxation time (IVRT) labeled. (E-G) Quantifications of IVRT (E), AET (F), and pressure within the VB valve (G) in male and female wild type and irf8 mutant fish at 6 and 12 mpf. Male n = 10-21 per genotype and timepoint. Female n = 9-14 per genotype and timepoint. Each point represents individual beats per fish. 8-10 beats were analyzed per fish. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (H) Quantification of heart in male and female wild type and irf8 mutants. (I) Representative echocardiogram traces from a wild type and irf8 mutant recording. (J-M) Quantification of diastasis time in wild type males (J) and females (K) at 6 mpf and 12 mpf, as well as diastasis time in irf8 mutant males (L) and females (M) at 6 mpf and 12 mpf. A Gaussian distribution was created by analyzing 8 beats per sample and grouping diastasis duration in defined increments. Male n = 10-21 per genotype and timepoint. Female n = 9-14 per genotype and timepoint. Horizontal bars represent the range of diastasis duration. (N-O) 95% confidence interval of the average diastasis time durations in wild type (gray) and irf8 mutant (blue) males (N) and females (O).

Article Snippet: To record electrical impulses, we used the CardioPhys TM ECG system (World Precision Instruments), which included a low-noise gold ECG probe, micromanipulator, bio-amplifier and ground electrode, in combination with the PowerLab 2/26 data acquisition unit (ADInstruments, PL2602).

Techniques: Labeling, Mutagenesis