Journal: Metabolomics

Article Title: Comparison of reversed-phase, hydrophilic interaction, and porous graphitic carbon chromatography columns for an untargeted toxicometabolomics study in pooled human liver microsomes, rat urine, and rat plasma

doi: 10.1007/s11306-024-02115-0

Figure Lengend Snippet: Overview of the used columns and their corresponding flow rates, gradients, and mobile phases

Article Snippet: Specification , Thermo Fisher Accucore Phenyl-Hexyl column , Thermo Fisher Hypersil Gold C 18 column , Waters ACQUITY UPLC BEH C 18 column , Macherey–Nagel HILIC Nucleodur column , Merck SeQuant ZIC HILIC , Merck PGC SupelTM Carbon LC.

Techniques: Hydrophilic Interaction Liquid Chromatography

Journal: Metabolomics

Article Title: Comparison of reversed-phase, hydrophilic interaction, and porous graphitic carbon chromatography columns for an untargeted toxicometabolomics study in pooled human liver microsomes, rat urine, and rat plasma

doi: 10.1007/s11306-024-02115-0

Figure Lengend Snippet: Overview of the analytical workflow used in this study. Sample types were prepared with different preparation methods; samples were then separated on different reversed-phase and hydrophilic interaction-phase columns; mass spectrometry acquisition was performed in positive and negative ionization mode; data processing and evaluation was done using an in-house R script based on XCMS; columns were compared in terms of their different outcomes. (Created with BioRender.com)

Article Snippet: Specification , Thermo Fisher Accucore Phenyl-Hexyl column , Thermo Fisher Hypersil Gold C 18 column , Waters ACQUITY UPLC BEH C 18 column , Macherey–Nagel HILIC Nucleodur column , Merck SeQuant ZIC HILIC , Merck PGC SupelTM Carbon LC.

Techniques: Mass Spectrometry

Journal: Metabolomics

Article Title: Comparison of reversed-phase, hydrophilic interaction, and porous graphitic carbon chromatography columns for an untargeted toxicometabolomics study in pooled human liver microsomes, rat urine, and rat plasma

doi: 10.1007/s11306-024-02115-0

Figure Lengend Snippet: Bar chart showing feature count detected after peak picking and their respective reproducibility evaluated by CV (coefficient of variation) in pooled human liver microsomes (HLM), rat urine, and rat plasma using different reversed-phase (A) and HILIC (B) columns. pos = positive, neg = negative, BEH = BEH C 18 , Gold = Hypersil Gold C 18 , Nucleodur = ammonium-sulfonic acid, ZicHILIC = sulfobetaine, PGC = porous graphitic carbon

Article Snippet: Specification , Thermo Fisher Accucore Phenyl-Hexyl column , Thermo Fisher Hypersil Gold C 18 column , Waters ACQUITY UPLC BEH C 18 column , Macherey–Nagel HILIC Nucleodur column , Merck SeQuant ZIC HILIC , Merck PGC SupelTM Carbon LC.

Techniques: Clinical Proteomics, Hydrophilic Interaction Liquid Chromatography

Journal: Metabolomics

Article Title: Comparison of reversed-phase, hydrophilic interaction, and porous graphitic carbon chromatography columns for an untargeted toxicometabolomics study in pooled human liver microsomes, rat urine, and rat plasma

doi: 10.1007/s11306-024-02115-0

Figure Lengend Snippet: Summary of study factors and the respective results from this study

Article Snippet: Specification , Thermo Fisher Accucore Phenyl-Hexyl column , Thermo Fisher Hypersil Gold C 18 column , Waters ACQUITY UPLC BEH C 18 column , Macherey–Nagel HILIC Nucleodur column , Merck SeQuant ZIC HILIC , Merck PGC SupelTM Carbon LC.

Techniques: