ebc-1 cell line Search Results


90
Creative Bioarray Inc human lung squamous cell ebc1
The MET PSI domain is indispensable for MET maturation through the CXXC motifs. ( A ) <t>EBC1</t> cells were treated with indicated concentrations of 16F16 for 48 h or DMSO as vehicle. Proteins were resolved in 6% SDS-PAGE, and MET expression was assessed by Western blot. ( B ) TOV112D cells stably expressing MET wt or MET∆PSI were lysed and proteins’ thiol groups were labelled according to their redox state as described in the . Ctrl condition corresponds to cells labelled with Cy3-conjugated maleimide only to remove autofluorescence in the 647 channel. Reduced thiols are presented with the green pseudocolor and oxidized thiols are shown with the red pseudocolor. TOV112D ( C ) and HEK293T ( D , E ) cells were transiently transfected with indicated constructs, and cells were lysed 48 h post-transfection to assess MET expression with MET and 6X_HIS tag antibodies. Β-ACTIN was used as a loading control. Western blots were quantified using ImageJ software v2.1.0/1.53c. Results are mean +/− SEM with N = 3. ANOVA with Tukey’s post hoc test was performed to compare different groups with ns: not-significant; **: p < 0.01; ****: p < 0.0001.
Human Lung Squamous Cell Ebc1, supplied by Creative Bioarray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
human lung squamous cell ebc1 - by Bioz Stars, 2026-02
90/100 stars
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90
Crown Bioscience human nsclc cell line ebc-1
The MET PSI domain is indispensable for MET maturation through the CXXC motifs. ( A ) <t>EBC1</t> cells were treated with indicated concentrations of 16F16 for 48 h or DMSO as vehicle. Proteins were resolved in 6% SDS-PAGE, and MET expression was assessed by Western blot. ( B ) TOV112D cells stably expressing MET wt or MET∆PSI were lysed and proteins’ thiol groups were labelled according to their redox state as described in the . Ctrl condition corresponds to cells labelled with Cy3-conjugated maleimide only to remove autofluorescence in the 647 channel. Reduced thiols are presented with the green pseudocolor and oxidized thiols are shown with the red pseudocolor. TOV112D ( C ) and HEK293T ( D , E ) cells were transiently transfected with indicated constructs, and cells were lysed 48 h post-transfection to assess MET expression with MET and 6X_HIS tag antibodies. Β-ACTIN was used as a loading control. Western blots were quantified using ImageJ software v2.1.0/1.53c. Results are mean +/− SEM with N = 3. ANOVA with Tukey’s post hoc test was performed to compare different groups with ns: not-significant; **: p < 0.01; ****: p < 0.0001.
Human Nsclc Cell Line Ebc 1, supplied by Crown Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human nsclc cell line ebc-1/product/Crown Bioscience
Average 90 stars, based on 1 article reviews
human nsclc cell line ebc-1 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


The MET PSI domain is indispensable for MET maturation through the CXXC motifs. ( A ) EBC1 cells were treated with indicated concentrations of 16F16 for 48 h or DMSO as vehicle. Proteins were resolved in 6% SDS-PAGE, and MET expression was assessed by Western blot. ( B ) TOV112D cells stably expressing MET wt or MET∆PSI were lysed and proteins’ thiol groups were labelled according to their redox state as described in the . Ctrl condition corresponds to cells labelled with Cy3-conjugated maleimide only to remove autofluorescence in the 647 channel. Reduced thiols are presented with the green pseudocolor and oxidized thiols are shown with the red pseudocolor. TOV112D ( C ) and HEK293T ( D , E ) cells were transiently transfected with indicated constructs, and cells were lysed 48 h post-transfection to assess MET expression with MET and 6X_HIS tag antibodies. Β-ACTIN was used as a loading control. Western blots were quantified using ImageJ software v2.1.0/1.53c. Results are mean +/− SEM with N = 3. ANOVA with Tukey’s post hoc test was performed to compare different groups with ns: not-significant; **: p < 0.01; ****: p < 0.0001.

Journal: International Journal of Molecular Sciences

Article Title: The PSI Domain of the MET Oncogene Encodes a Functional Disulfide Isomerase Essential for the Maturation of the Receptor Precursor

doi: 10.3390/ijms232012427

Figure Lengend Snippet: The MET PSI domain is indispensable for MET maturation through the CXXC motifs. ( A ) EBC1 cells were treated with indicated concentrations of 16F16 for 48 h or DMSO as vehicle. Proteins were resolved in 6% SDS-PAGE, and MET expression was assessed by Western blot. ( B ) TOV112D cells stably expressing MET wt or MET∆PSI were lysed and proteins’ thiol groups were labelled according to their redox state as described in the . Ctrl condition corresponds to cells labelled with Cy3-conjugated maleimide only to remove autofluorescence in the 647 channel. Reduced thiols are presented with the green pseudocolor and oxidized thiols are shown with the red pseudocolor. TOV112D ( C ) and HEK293T ( D , E ) cells were transiently transfected with indicated constructs, and cells were lysed 48 h post-transfection to assess MET expression with MET and 6X_HIS tag antibodies. Β-ACTIN was used as a loading control. Western blots were quantified using ImageJ software v2.1.0/1.53c. Results are mean +/− SEM with N = 3. ANOVA with Tukey’s post hoc test was performed to compare different groups with ns: not-significant; **: p < 0.01; ****: p < 0.0001.

Article Snippet: The human ovarian carcinoma TOV112D (RRID:CVCL_3612, ATCC CRL-11731TM), the human lung squamous cell EBC1 (RRID:CVCL_2891, Creative Bioarray CSC-C6336J), and the human embryonic kidney HEK293T (RRID:CVCL_0063, ATCC CRL-1573TM) cell lines were purchased and cultured according to manufacturer’s instructions in a humidified atmosphere at 37 °C.

Techniques: SDS Page, Expressing, Western Blot, Stable Transfection, Transfection, Construct, Control, Software