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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: The PSI Domain of the MET Oncogene Encodes a Functional Disulfide Isomerase Essential for the Maturation of the Receptor Precursor
doi: 10.3390/ijms232012427
Figure Lengend Snippet: The MET PSI domain is indispensable for MET maturation through the CXXC motifs. ( A ) EBC1 cells were treated with indicated concentrations of 16F16 for 48 h or DMSO as vehicle. Proteins were resolved in 6% SDS-PAGE, and MET expression was assessed by Western blot. ( B ) TOV112D cells stably expressing MET wt or MET∆PSI were lysed and proteins’ thiol groups were labelled according to their redox state as described in the . Ctrl condition corresponds to cells labelled with Cy3-conjugated maleimide only to remove autofluorescence in the 647 channel. Reduced thiols are presented with the green pseudocolor and oxidized thiols are shown with the red pseudocolor. TOV112D ( C ) and HEK293T ( D , E ) cells were transiently transfected with indicated constructs, and cells were lysed 48 h post-transfection to assess MET expression with MET and 6X_HIS tag antibodies. Β-ACTIN was used as a loading control. Western blots were quantified using ImageJ software v2.1.0/1.53c. Results are mean +/− SEM with N = 3. ANOVA with Tukey’s post hoc test was performed to compare different groups with ns: not-significant; **: p < 0.01; ****: p < 0.0001.
Article Snippet: The human ovarian carcinoma TOV112D (RRID:CVCL_3612, ATCC CRL-11731TM), the
Techniques: SDS Page, Expressing, Western Blot, Stable Transfection, Transfection, Construct, Control, Software