Journal: Cell reports

Article Title: TIFAB Regulates USP15-Mediated p53 Signaling during Stressed and Malignant Hematopoiesis

doi: 10.1016/j.celrep.2020.01.093

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: For colony forming assays in methylcellulose, isolation and enrichment of hematopoietic stem/progenitor cells (HSPCs) from Tifab+/+ and Tifab−/− mice was performed using the EasyStep Mouse Hematopoietic Progenitor Cell Enrichment Kit (Stem Cell Technologies 19856) for lineage depletion, or by AutoMACS positive selection using CD117 murine microbeads for ckit+ enrichment.

Techniques: Ubiquitin Proteomics, Virus, Plasmid Preparation, Recombinant, Silver Staining, Transfection, Purification, Lysis, Microarray, Negative Control, Software

Journal: American Journal of Cancer Research

Article Title: cAMP induces cell apoptosis in multiple myeloma and overcomes bortezomib resistance

doi:

Figure Lengend Snippet: The combination of 8-CPT-cAMP and bortezomib synergistically induced apoptosis in primary CD138+ MM cells. A. Primary CD138+ cells isolated from MM patients were exposed to 8-CPT-cAMP, bortezomib, or their combinations for 24 h; cell apoptosis was evaluated by Annexin V/PI staining; B. CD138+ MM cell co-cultured with bone marrow stromal cells (BMSCs) for 24 h and treated with 8-CPT-cAMP, bortezomib, or their combination for 24 h; cell apoptosis was evaluated by Annexin V/PI staining. *P < 0.05, **P < 0.01. The experiments were performed in triplicate.

Article Snippet: CD138 + cells were isolated from MNCs using EasyStep CD138 + magnetic nanoparticles (Stem Cell Technologies, Vancouver, BC, Canada) according to the manufacturer’s instructions.

Techniques: Isolation, Staining, Cell Culture