Journal: Experimental & Molecular Medicine

Article Title: Obesity aggravates the joint inflammation in a collagen-induced arthritis model through deviation to Th17 differentiation

doi: 10.3858/emm.2012.44.7.047

Figure Lengend Snippet: The antigen-specific autoantibodies increased in the obese CIA mice. Ten weeks after the second immunization, sera from the obese CIA and CIA mice ( n = 6 for each group) were collected. The CII-specific total IgG (A) and IgG2a (B) concentrations were determined in the serum samples of the individual mice by ELISA. The data is expressed as the mean ± SD. * P < 0.01 compared with the CIA mice.

Article Snippet: The concentrations of IgG2a and IgG1 were measured using mouse IgG2a and IgG1 ELISA Quantitation Kits (Bethyl Laboratories, Montgomery, TX), respectively.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The lupus susceptibility locus Sle1 facilitates the peripheral development and selection of anti-DNA B cells through impaired receptor editing.

doi: 10.4049/jimmunol.1201558

Figure Lengend Snippet: FIGURE 1. Serum levels of IgM and IgG2a Abs to nuclear Ags in B6.56R and B6.Sle1.56R mice. Sera from 9–12-mo-old female B6.56R mice (s, n = 11) and B6.Sle1.56R mice (d, n = 12) were assayed for levels of IgM or IgG anti-ssDNA and anti-dsDNA Abs using ELISA. Each symbol represents a single mouse. Horizontal lines indicate the mean Ab levels in each strain. Mouse IgM and IgG Abs were used as standard control. The p values were obtained using Student t test comparison of B6.Sle1.56R data with the corresponding B6.56R data. The “a” denotes allotype originated from 56R VH chain (backcrossed with BALB/c).

Article Snippet: Mouse IgM and IgG ELISA Quantitative Kits (Bethyl Laboratories, Montgomery, TX) were used as a standard control.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Comparison