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Johns Hopkins HealthCare
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Cold Spring Harbor Laboratory Meetings
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Image Search Results
Journal: Journal of Virology
Article Title: Suppression of Adenovirus Replication by Cardiotonic Steroids
doi: 10.1128/JVI.01623-16
Figure Lengend Snippet: Effect of digoxin and digitoxin on adenoviral E4orf6 protein expression. A549 cells were infected with HAdV-C5, treated with digoxin or digitoxin immediately after the adsorption period, and then fixed at 8 h p.i. (A) and at 24 h p.i. (B) for immunodetection of E4orf6. Nuclei are stained with DAPI. The proportion of cells positive for E4orf6 protein expression is based on total counts of ∼500 cells in 12 to 14 random fields for each condition in panel A and 300 to 400 cells in 8 or 9 random fields for each condition in panel B. Error bars show the standard error of the mean from two (B) or three (A) experiments. An unpaired, 2-tailed Student t test was used to determine P values.
Article Snippet: We appreciate the gift of
Techniques: Expressing, Infection, Adsorption, Immunodetection, Staining