Journal: International journal of medical sciences

Article Title: Ube2S regulates Wnt/β-catenin signaling and promotes the progression of non-small cell lung cancer.

doi: 10.7150/ijms.40243

Figure Lengend Snippet: Figure 1. Immunostaining of Ube2S in normal lung tissues and NSCLC tissues. Bronchial epithelial cells (black arrow) and alveolar cells (grey arrow) show negative immunostaining for Ube2S (A). Bronchial epithelial cells (black arrow) and submucosal glands (grey arrow) show negative immunostaining for Ube2S (B). Weak and focal immunostaining of Ube2S is evident in some bronchial epithelial cells (C) and submucosal glands (D). Strong immunostaining of Ube2S is present in the cytoplasm and nuclei of squamous cell carcinoma (E) and adenocarcinoma (F) cells. (A: ×100; B, D, F: ×200; C, E: ×400)

Article Snippet: Ube2S cDNA clone was purchased from Origene (Rockville, MD, USA).

Techniques: Immunostaining

Journal: International journal of medical sciences

Article Title: Ube2S regulates Wnt/β-catenin signaling and promotes the progression of non-small cell lung cancer.

doi: 10.7150/ijms.40243

Figure Lengend Snippet: Figure 2. Survival function. Ube2S expression in non-small cell lung cancer is significantly associated with poor patient survival (34.9 ± 5.5 versus 56.4 ± 7.3 months) (Log rank test, p < 0.05)

Article Snippet: Ube2S cDNA clone was purchased from Origene (Rockville, MD, USA).

Techniques: Expressing

Journal: International journal of medical sciences

Article Title: Ube2S regulates Wnt/β-catenin signaling and promotes the progression of non-small cell lung cancer.

doi: 10.7150/ijms.40243

Figure Lengend Snippet: Figure 3. The function of Ube2S in lung cancer cells. Western blots show that Ube2S is expressed in bronchial epithelial HBE cells, and lung cancer A549 and NCI-H1299 cells (A). The MTT assay shows that overexpression of Ube2S in A549 cells significantly promotes cancer cell proliferation (B) (p < 0.05). The wound scratch healing assay shows that overexpression of Ube2S in A549 cells significantly promotes cancer cell migration (C) (*p < 0.05)

Article Snippet: Ube2S cDNA clone was purchased from Origene (Rockville, MD, USA).

Techniques: Western Blot, MTT Assay, Over Expression, Migration

Journal: International journal of medical sciences

Article Title: Ube2S regulates Wnt/β-catenin signaling and promotes the progression of non-small cell lung cancer.

doi: 10.7150/ijms.40243

Figure Lengend Snippet: Figure 4. Ube2S regulates Wnt/β-catenin signaling molecules and activity. Western blots show that overexpression of Ube2S in A549 cells significantly upregulates Wnt/β-catenin signaling molecules including β-catenin, cyclin D1, and MMP7 (A) (p < 0.05). The luciferase assay shows that overexpression of Ube2S in A549 cells significantly upregulates Wnt/β-catenin signaling activity (B) (p < 0.05)

Article Snippet: Ube2S cDNA clone was purchased from Origene (Rockville, MD, USA).

Techniques: Activity Assay, Western Blot, Over Expression, Luciferase

Journal: International journal of medical sciences

Article Title: Ube2S regulates Wnt/β-catenin signaling and promotes the progression of non-small cell lung cancer.

doi: 10.7150/ijms.40243

Figure Lengend Snippet: Figure 5. Wnt/β-catenin signaling inhibitor abolished the function of Ube2s in lung cancer cells. The MTT assay shows that addition of the Wnt/β-catenin signaling inhibitor, ETC-159, significantly inhibits the ability of Ube2S to promote cancer cell proliferation

Article Snippet: Ube2S cDNA clone was purchased from Origene (Rockville, MD, USA).

Techniques: MTT Assay

Journal: The Journal of Biological Chemistry

Article Title: An E2-guided E3 Screen Identifies the RNF17-UBE2U Pair as Regulator of the Radiosensitivity, Immunodeficiency, Dysmorphic Features, and Learning Difficulties (RIDDLE) Syndrome Protein RNF168 *

doi: 10.1074/jbc.M116.758854

Figure Lengend Snippet: Sequences of siRNAs

Article Snippet: The siRNA libraries targeting 37 E2s and 64 UBE2U-interacting E3s were purchased from GenePharma ( ). siRNAs for cell depletion of UBC13, RNF8, and RNF168 were described previously ( 31 , 56 ).

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: An E2-guided E3 Screen Identifies the RNF17-UBE2U Pair as Regulator of the Radiosensitivity, Immunodeficiency, Dysmorphic Features, and Learning Difficulties (RIDDLE) Syndrome Protein RNF168 *

doi: 10.1074/jbc.M116.758854

Figure Lengend Snippet: Screen of UBE2U-interacting E3s identifies novel regulators of DSB responses. A, network illustration of interactions between UBE2U and its potential E3 partners (35, 36). B and C, U2OS cells transfected with an E3-targeting siRNA library or the indicated siRNAs were irradiated (10 Gy). 4 h post-IR treatment, cells were fixed, permeabilized, and immunostained with antibodies against 53BP1 and γH2AX, and the percentage of 53BP1 focus-positive cells (>10 foci per cell) was quantified. Ranking by z score of all samples was plotted as described under “Experimental Procedures.” Representative images following RNAi-mediated depletion of RNF17, TRIM34, TRIM65, or RNF10 are shown (C). Non-targeting siRNAs (siCTR) or RNF168-targeting siRNAs (siRNF168) were used as negative and positive controls, respectively.

Article Snippet: The siRNA libraries targeting 37 E2s and 64 UBE2U-interacting E3s were purchased from GenePharma ( ). siRNAs for cell depletion of UBC13, RNF8, and RNF168 were described previously ( 31 , 56 ).

Techniques: Transfection, Irradiation