Journal: PLoS ONE

Article Title: Exploring Genetic Factors Involved in Huntington Disease Age of Onset: E2F2 as a New Potential Modifier Gene

doi: 10.1371/journal.pone.0131573

Figure Lengend Snippet: Multiple linear regression analysis between SNP genotypes and eAO.

Article Snippet: For Taqman-based assays, Hs00918089_m1 probe, which hybridizes with the 3–4 exon boundary of the E2F2 gene, was used.

Techniques:

Journal: PLoS ONE

Article Title: Exploring Genetic Factors Involved in Huntington Disease Age of Onset: E2F2 as a New Potential Modifier Gene

doi: 10.1371/journal.pone.0131573

Figure Lengend Snippet: Multiple linear regression analysis between SNP genotypes and mAO.

Article Snippet: For Taqman-based assays, Hs00918089_m1 probe, which hybridizes with the 3–4 exon boundary of the E2F2 gene, was used.

Techniques:

Journal: PLoS ONE

Article Title: Exploring Genetic Factors Involved in Huntington Disease Age of Onset: E2F2 as a New Potential Modifier Gene

doi: 10.1371/journal.pone.0131573

Figure Lengend Snippet: Two methods were used. In Taqman assay, the expression of E2F2 gene was analyzed in 31 samples (N TT = 4; N GT = 12, N GG = 15) with Hs00918089_m1 Taqman probe; the expression values were normalized respect to expression of B2M and YWHAZ reference genes. In SYBR Green assay, the E2F2 gene expression was estimated in 31 samples (N TT = 5; N GT = 14, N GG = 12); the expression values were normalized to expression of UBC and YWHAZ reference genes. Results are expressed as fold over respective GG individuals. Asterisk denotes statistically significant differences (P<0.05) between GG and any other group, according to DataAssist software analysis (T-test) or REST software analysis (Pair Wise Reallocation Randomization test).

Article Snippet: For Taqman-based assays, Hs00918089_m1 probe, which hybridizes with the 3–4 exon boundary of the E2F2 gene, was used.

Techniques: TaqMan Assay, Expressing, SYBR Green Assay, Gene Expression, Software