e. coli xl1-blue cells Search Results


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  • 99
    ATCC competent e coli
    Competent E Coli, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher transformation competent e coli
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    Agilent technologies e coli xl1 blue cells
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    Stratagene e coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Thermo Fisher escherichia coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
    Escherichia Coli Xl1 Blue Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene competent e coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Agilent technologies competent e coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Stratagene electrocompetent e coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Thermo Fisher electrocompetent e coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Millipore competent e coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    GE Healthcare e coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Millipore e coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
    E Coli Xl1 Blue Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher competent e coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Stratagene ultracompetent escherichia coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Stratagene supercompetent e coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Agilent technologies electrocompetent e coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Stratagene log phase e coli xl1 blue cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
    Log Phase E Coli Xl1 Blue Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies e coli xl1 blue supercompetent cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Stratagene e coli xl1 blue mrf cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
    E Coli Xl1 Blue Mrf Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene e coli xl1 blue p2 cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
    E Coli Xl1 Blue P2 Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene escherichia coli xl1 blue mrf host cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
    Escherichia Coli Xl1 Blue Mrf Host Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene competent escherichia coli xl1 blue mrf cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
    Competent Escherichia Coli Xl1 Blue Mrf Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene electrocompetent e coli xl1 blue mrf cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Agilent technologies e coli xl1 blue electroporation competent cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
    E Coli Xl1 Blue Electroporation Competent Cells, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa infected escherichia coli xl1 blue bacterial cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
    Infected Escherichia Coli Xl1 Blue Bacterial Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene e coli xl1 blue host cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
    E Coli Xl1 Blue Host Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 81/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore e coli xl1 blue supercompetent cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Agilent technologies e coli xl1 blue chemical competent cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Stratagene escherichia coli xl1 blue mrf supercompetent cells
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Stratagene e coli xl1 blue strain
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Becton Dickinson e coli xl1 blue
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Agilent technologies e coli xl1 blue strain
    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli <t>XL1</t> Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.
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    Image Search Results


    Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli XL1 Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.

    Journal: Immunology

    Article Title: Phage display of human antibodies from a patient suffering from coeliac disease and selection of isotype-specific scFv against gliadin

    doi: 10.1046/j.1365-2567.2003.01728.x

    Figure Lengend Snippet: Isotype-specific phage enrichment during subsequent rounds of affinity selection. The number of phage was determined as colony-forming units (CFU) after infection of freshly grown Escherichia coli XL1 Blue cells. Notably, the absolute number of eluted phage significantly depends on the isotype library used for screening.

    Article Snippet: Bound phage were eluted with 0·5 ml of 0·1- m HCl (pH 2·2 adjusted with solid glycine), neutralized with 60 µl of 1- m Tris–HCl (pH 8·0) and used for infection of freshly cultured, exponentially growing E. coli XL1 Blue cells.

    Techniques: Selection, Infection

    Low-temperature growth of E. coli transformants harboring the icdII gene. (Upper panel) E. coli XL1-Blue was transformed with pMSF8 or pMS42. (Lower panel) E. coli DEK2004 was used as the host and transformed with the indicated plasmid. pBluescript was used as a vector. E. coli transformants harboring each plasmid were streaked on MOPS-succinate or MOPS-succinate-yeast extract agar plates and incubated at 10 or 15°C. Photographs were taken 2 weeks after the inoculation.

    Journal: Journal of Bacteriology

    Article Title: cis-Acting Elements Responsible for Low-Temperature-Inducible Expression of the Gene Coding for the Thermolabile Isocitrate Dehydrogenase Isozyme of a Psychrophilic Bacterium, Vibrio sp. Strain ABE-1

    doi:

    Figure Lengend Snippet: Low-temperature growth of E. coli transformants harboring the icdII gene. (Upper panel) E. coli XL1-Blue was transformed with pMSF8 or pMS42. (Lower panel) E. coli DEK2004 was used as the host and transformed with the indicated plasmid. pBluescript was used as a vector. E. coli transformants harboring each plasmid were streaked on MOPS-succinate or MOPS-succinate-yeast extract agar plates and incubated at 10 or 15°C. Photographs were taken 2 weeks after the inoculation.

    Article Snippet: E. coli DEK2004, a mutant defective in icd (a gift from Peter Thorsness), or E. coli XL1-Blue (Stratagene) was used as a host for transformation by icd genes.

    Techniques: Transformation Assay, Plasmid Preparation, Incubation

    Growth of E. coli transformants harboring 5′-deleted icdII or an icdII-icdI fusion gene. (A) E. coli DEK2004 was transformed with vector [pBluescript KS(+)] (○), pMS42 (▿), pIS202 (▴), or pMSF8 (▵). (B) E. coli DEK2004 was transformed with pTS601 (⧫), pTS602 (◊), or pSS512 (□). As a control, E. coli XL1-Blue, which has its own icd gene, was also transformed with the vector (●). Other symbols are the same as in panel A. Each transformant, precultured in LB medium at 37°C for 16 h, was inoculated in fresh LB medium and cultured at 15 or 37°C. (C) Specific activity of IDH determined with crude extract prepared from each transformant. Details of assay conditions are described in Materials and Methods. Note the thermolability of IDH-II at 37°C.

    Journal: Journal of Bacteriology

    Article Title: cis-Acting Elements Responsible for Low-Temperature-Inducible Expression of the Gene Coding for the Thermolabile Isocitrate Dehydrogenase Isozyme of a Psychrophilic Bacterium, Vibrio sp. Strain ABE-1

    doi:

    Figure Lengend Snippet: Growth of E. coli transformants harboring 5′-deleted icdII or an icdII-icdI fusion gene. (A) E. coli DEK2004 was transformed with vector [pBluescript KS(+)] (○), pMS42 (▿), pIS202 (▴), or pMSF8 (▵). (B) E. coli DEK2004 was transformed with pTS601 (⧫), pTS602 (◊), or pSS512 (□). As a control, E. coli XL1-Blue, which has its own icd gene, was also transformed with the vector (●). Other symbols are the same as in panel A. Each transformant, precultured in LB medium at 37°C for 16 h, was inoculated in fresh LB medium and cultured at 15 or 37°C. (C) Specific activity of IDH determined with crude extract prepared from each transformant. Details of assay conditions are described in Materials and Methods. Note the thermolability of IDH-II at 37°C.

    Article Snippet: E. coli DEK2004, a mutant defective in icd (a gift from Peter Thorsness), or E. coli XL1-Blue (Stratagene) was used as a host for transformation by icd genes.

    Techniques: Transformation Assay, Plasmid Preparation, Cell Culture, Activity Assay

    Optimization of expression conditions and screening background. (A) Colonies of E . coli BL21 transformed with TN-XXL cloned into the vector pRSETB (exposure time 4s, gain 2x, binning 1). Scale bar, 10 mm (B) Colonies of E . coli XL1 transformed with TN-XXL cloned into pRSETB and imaged under the same conditions. (C) Mean fluorescence intensities ± SD of colonies transformed with TN-XXL in either BL1 or XL1, imaged under identical conditions (n BL21 = 312, n XL1 = 558). (D) ΔR/R ± SD of E . coli colonies expressing TN-XXL cloned into BL21 or XL1 (n BL21 = 19, n XL1 = 15). (E) Auto-fluorescence of agar plate versus white blotting paper imaged under identical conditions with filters used for FRET imaging. Error bars indicate SD. (F) Basal ratio valuesR 0 ± SD of XL1 colonies transformed with the FRET sensor TN-XXL and imaged on agar plate or on filter paper. (n plate = 149 colonies, n blotting paper = 90 colonies) (G) ΔR/R ± SD of the same colonies following calcium application.

    Journal: PLoS ONE

    Article Title: Large Scale Bacterial Colony Screening of Diversified FRET Biosensors

    doi: 10.1371/journal.pone.0119860

    Figure Lengend Snippet: Optimization of expression conditions and screening background. (A) Colonies of E . coli BL21 transformed with TN-XXL cloned into the vector pRSETB (exposure time 4s, gain 2x, binning 1). Scale bar, 10 mm (B) Colonies of E . coli XL1 transformed with TN-XXL cloned into pRSETB and imaged under the same conditions. (C) Mean fluorescence intensities ± SD of colonies transformed with TN-XXL in either BL1 or XL1, imaged under identical conditions (n BL21 = 312, n XL1 = 558). (D) ΔR/R ± SD of E . coli colonies expressing TN-XXL cloned into BL21 or XL1 (n BL21 = 19, n XL1 = 15). (E) Auto-fluorescence of agar plate versus white blotting paper imaged under identical conditions with filters used for FRET imaging. Error bars indicate SD. (F) Basal ratio valuesR 0 ± SD of XL1 colonies transformed with the FRET sensor TN-XXL and imaged on agar plate or on filter paper. (n plate = 149 colonies, n blotting paper = 90 colonies) (G) ΔR/R ± SD of the same colonies following calcium application.

    Article Snippet: Bacterial plate screening Libraries were transformed into E .coli XL1-Blue cells or E .coli BL21-Gold cells (both Stratagene) and plated on LB agar plates containing ampicillin (100 μg/ml) with a desired colony density of ~700–800 colonies per plate.

    Techniques: Expressing, Transformation Assay, Clone Assay, Plasmid Preparation, Fluorescence, Imaging

    Permeabilizing E . coli to exogenous calcium (A) Average ΔR/R ± SD of a FRET sensor (Twitch-1) expressed in XL1 cells pretreated with poly-L-lysine and ionomycin and subjected to different concentrations of extracellular solution calcium (n = 6 colonies per concentration). (B-C) FRET trace (± standard error of the mean, grey) and individual donor and acceptor traces (± standard error of the mean, grey) of colonies of E . coli XL1 transformed with TN-XXL. Arrows indicate when calcium was applied. (n 100mM = 197 colonies, n 1M = 222 colonies). (D-E) Representative ΔR/R values following calcium application (100 mM) of colonies pretreated with different concentrations of either poly-L-lysine (PL) or ionomycin (IM), while the concentration of the second substance was kept constant. Grey dashed lines represent signal changes after the application of poly-L-lysine or ionomycin, black lines represent additional signal change after the application of calcium, and dark lines indicate total signal changes.

    Journal: PLoS ONE

    Article Title: Large Scale Bacterial Colony Screening of Diversified FRET Biosensors

    doi: 10.1371/journal.pone.0119860

    Figure Lengend Snippet: Permeabilizing E . coli to exogenous calcium (A) Average ΔR/R ± SD of a FRET sensor (Twitch-1) expressed in XL1 cells pretreated with poly-L-lysine and ionomycin and subjected to different concentrations of extracellular solution calcium (n = 6 colonies per concentration). (B-C) FRET trace (± standard error of the mean, grey) and individual donor and acceptor traces (± standard error of the mean, grey) of colonies of E . coli XL1 transformed with TN-XXL. Arrows indicate when calcium was applied. (n 100mM = 197 colonies, n 1M = 222 colonies). (D-E) Representative ΔR/R values following calcium application (100 mM) of colonies pretreated with different concentrations of either poly-L-lysine (PL) or ionomycin (IM), while the concentration of the second substance was kept constant. Grey dashed lines represent signal changes after the application of poly-L-lysine or ionomycin, black lines represent additional signal change after the application of calcium, and dark lines indicate total signal changes.

    Article Snippet: Bacterial plate screening Libraries were transformed into E .coli XL1-Blue cells or E .coli BL21-Gold cells (both Stratagene) and plated on LB agar plates containing ampicillin (100 μg/ml) with a desired colony density of ~700–800 colonies per plate.

    Techniques: Concentration Assay, Transformation Assay