Journal: mSystems

Article Title: Characterization of the Plasmidome Encoding Carbapenemase and Mechanisms for Dissemination of Carbapenem-Resistant Enterobacteriaceae

doi: 10.1128/mSystems.00759-20

Figure Lengend Snippet: MICs of meropenem and conjugation efficiency in transformants with plasmids from representative isolates in each group ^a

Article Snippet: , IncF , E305 , <1 , (7.5 ± 2.3) × 10 −4.

Techniques: Conjugation Assay

Journal: mSystems

Article Title: Characterization of the Plasmidome Encoding Carbapenemase and Mechanisms for Dissemination of Carbapenem-Resistant Enterobacteriaceae

doi: 10.1128/mSystems.00759-20

Figure Lengend Snippet: Comparison of E. coli isolates E305 and E318 ^a

Article Snippet: , IncF , E305 , <1 , (7.5 ± 2.3) × 10 −4.

Techniques: Comparison, Plasmid Preparation

Journal: mSystems

Article Title: Characterization of the Plasmidome Encoding Carbapenemase and Mechanisms for Dissemination of Carbapenem-Resistant Enterobacteriaceae

doi: 10.1128/mSystems.00759-20

Figure Lengend Snippet: Genomic structure of group IncF plasmid pE305_IMP6 and enhanced transcription of bla IMP-6 . (A) Genomic structure of plasmid pE305_IMP6. De novo assembly graph of plasmid pE305_IMP6 visualized by Bandage displays the connections between contigs. The length and depth of each contig are shown. Contig2 connects Contig1 with Contig3 or Contig4, and Contig5 connects Contig6 with Contig3 or Contig4. (B) Sizes of plasmids pE305_IMP6 and pE318_IMP6. PFGE of S1-digested genomic DNA from E. coli isolates E305 and E318, followed by Southern blotting with a bla IMP-6 probe, indicated the size of each plasmid. M, DNA size marker (lambda ladder; Bio-Rad). (C) Hypothetical structure of pE305_IMP6. The colors correspond to the colors of contigs in panel A. (D) Genomic comparison of pE318_IMP6 and hypothetical pE305_IMP6 single . According to the overlap between contigs of pE305_IMP6, the hypothetical sequence shown was assembled and compared to the sequence of plasmid pE318_IMP6. Except for the repeats, pE305_IMP6 single and pE318_IMP6 were highly similar. Block arrows indicate confirmed or putative ORFs and their orientations. The color code, arrows, and similarity are as described in the legend of Fig. 2 . The colors under arrows of pE305_IMP6 single correspond to the colors of contigs in panel A. (E) Transcript levels of bla IMP-6 in E. coli isolates E305 and E318. qPCR revealed significantly higher transcription of bla IMP-6 in isolate E305 than in isolate E318. The bar chart represents the mRNA transcript ratio of bla IMP-6 to the housekeeping gene rrsA , which was used as a reference gene. Bars indicate means ± standard deviations, calculated from sextuplet experiments. The P value was calculated by using the Mann-Whitney U test.

Article Snippet: , IncF , E305 , <1 , (7.5 ± 2.3) × 10 −4.

Techniques: Plasmid Preparation, Southern Blot, Marker, Comparison, Sequencing, Blocking Assay, MANN-WHITNEY