Journal: BMC Plant Biology
Article Title: A repeat region from the Brassica juncea HMA4 gene BjHMA4R is specifically involved in Cd2+ binding in the cytosol under low heavy metal concentrations
Figure Lengend Snippet: Growth of E. coli expressing BjHMA4R . BL21 transformants contained a pEASY-Blunt E1 expression vector (negative control) and BjHMA4R , respectively. The cultures were adjusted to an OD 600 of 1 and were serially diluted 10-fold in water. Five-microliter aliquots of each dilution were spotted either on nonselective LB plates or on LB plates supplemented with 600 μM CdCl 2 and 2.5 mM ZnCl 2, Ni(NO3) 2 , Co(NO3) 2 and Pb(NO3) 2 . After 1 day of incubation at 37 °C, the plates were imaged. The dilutions are indicated in the above figure, and three individual clones of each E. coli transformant were analyzed
Article Snippet: The E. coli strain BL21 was part of a commercial batch from Transgen Biotech, China.
Techniques: Expressing, Plasmid Preparation, Negative Control, Incubation, Clone Assay