dy3947 Search Results


human thrombomodulin duoset elisa  (R&D Systems)
93
R&D Systems human thrombomodulin duoset elisa
Assessments of mechanisms leading to increased sTM levels following stimulation with S. aureus proteins. HUVEC monolayers were stimulated for 22 h with supernatant of overnight cultures of clinically isolated strains of S. aureus (M37, P08) and α-toxin. sTM was assessed in the host cell culture supernatant of stimulated cells (A). <t>Thrombomodulin</t> gene (THBD) expression in HUVECs after stimulation was assessed by qPCR (B) and total TM levels were measured after cell lysis of stimulated cells (C). LDH and TM were measured in cell culture supernatants after stimulations, with and without addition of 1300 nM broad-spectrum MMP inhibitor Marimastat (MMPI) or 10,6 µM ADAM10 selective inhibitor GI254023X (A10I) (D-E). LDH release measurements indicate cytotoxicity. Quantification of sTM and LDH are given in corrected optical density values. Asterisks indicate statistically significant differences (* = p < 0.05, ** = p < 0.01, *** = p < 0.001) according to ANOVA followed by Fisher’s least significant difference (LSD) post hoc test (A, B, C) or student t-test (D, E). Colors indicate different stimuli and symbols indicate different biological replicates. Direct cleavage of 40 ng rTM by 100 ng rADAM10 (rA10) was evaluated by western blot after 2 h incubation at 37 °C, with or without MMPI or A10I (F). Cleavage products are indicated by arrows at approximately 60 and 40 kDa.
Human Thrombomodulin Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human thrombomodulin duoset elisa/product/R&D Systems
Average 93 stars, based on 1 article reviews
human thrombomodulin duoset elisa - by Bioz Stars, 2026-05
93/100 stars
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thrombomodulin concentration  (R&D Systems)
91
R&D Systems thrombomodulin concentration
Distribution of biomarker concentrations in plasma samples from cases with MPM and controls from Mexico
Thrombomodulin Concentration, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thrombomodulin concentration/product/R&D Systems
Average 91 stars, based on 1 article reviews
thrombomodulin concentration - by Bioz Stars, 2026-05
91/100 stars
  Buy from Supplier

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Assessments of mechanisms leading to increased sTM levels following stimulation with S. aureus proteins. HUVEC monolayers were stimulated for 22 h with supernatant of overnight cultures of clinically isolated strains of S. aureus (M37, P08) and α-toxin. sTM was assessed in the host cell culture supernatant of stimulated cells (A). Thrombomodulin gene (THBD) expression in HUVECs after stimulation was assessed by qPCR (B) and total TM levels were measured after cell lysis of stimulated cells (C). LDH and TM were measured in cell culture supernatants after stimulations, with and without addition of 1300 nM broad-spectrum MMP inhibitor Marimastat (MMPI) or 10,6 µM ADAM10 selective inhibitor GI254023X (A10I) (D-E). LDH release measurements indicate cytotoxicity. Quantification of sTM and LDH are given in corrected optical density values. Asterisks indicate statistically significant differences (* = p < 0.05, ** = p < 0.01, *** = p < 0.001) according to ANOVA followed by Fisher’s least significant difference (LSD) post hoc test (A, B, C) or student t-test (D, E). Colors indicate different stimuli and symbols indicate different biological replicates. Direct cleavage of 40 ng rTM by 100 ng rADAM10 (rA10) was evaluated by western blot after 2 h incubation at 37 °C, with or without MMPI or A10I (F). Cleavage products are indicated by arrows at approximately 60 and 40 kDa.

Journal: Virulence

Article Title: Staphylococcus aureus toxins mediate endothelial Thrombomodulin release during severe invasive infections

doi: 10.1080/21505594.2025.2605767

Figure Lengend Snippet: Assessments of mechanisms leading to increased sTM levels following stimulation with S. aureus proteins. HUVEC monolayers were stimulated for 22 h with supernatant of overnight cultures of clinically isolated strains of S. aureus (M37, P08) and α-toxin. sTM was assessed in the host cell culture supernatant of stimulated cells (A). Thrombomodulin gene (THBD) expression in HUVECs after stimulation was assessed by qPCR (B) and total TM levels were measured after cell lysis of stimulated cells (C). LDH and TM were measured in cell culture supernatants after stimulations, with and without addition of 1300 nM broad-spectrum MMP inhibitor Marimastat (MMPI) or 10,6 µM ADAM10 selective inhibitor GI254023X (A10I) (D-E). LDH release measurements indicate cytotoxicity. Quantification of sTM and LDH are given in corrected optical density values. Asterisks indicate statistically significant differences (* = p < 0.05, ** = p < 0.01, *** = p < 0.001) according to ANOVA followed by Fisher’s least significant difference (LSD) post hoc test (A, B, C) or student t-test (D, E). Colors indicate different stimuli and symbols indicate different biological replicates. Direct cleavage of 40 ng rTM by 100 ng rADAM10 (rA10) was evaluated by western blot after 2 h incubation at 37 °C, with or without MMPI or A10I (F). Cleavage products are indicated by arrows at approximately 60 and 40 kDa.

Article Snippet: The Human Thrombomodulin DuoSet ELISA (R&D Systems, DY3947) was used according to the manufacturer’s instructions to measure sTM levels.

Techniques: Isolation, Cell Culture, Expressing, Lysis, Western Blot, Incubation

Distribution of biomarker concentrations in plasma samples from cases with MPM and controls from Mexico

Journal: International Journal of Medical Sciences

Article Title: Biomarkers for Predicting Malignant Pleural Mesothelioma in a Mexican Population

doi: 10.7150/ijms.23939

Figure Lengend Snippet: Distribution of biomarker concentrations in plasma samples from cases with MPM and controls from Mexico

Article Snippet: Thrombomodulin concentration was determined according to the manufacturer´s instructions (DY3947, R&D Systems).

Techniques: Biomarker Assay, Significance Assay

Odds ratios from logistic regression analyses as estimates of the relative risk for an MPM based on the plasma concentrations in cases and controls

Journal: International Journal of Medical Sciences

Article Title: Biomarkers for Predicting Malignant Pleural Mesothelioma in a Mexican Population

doi: 10.7150/ijms.23939

Figure Lengend Snippet: Odds ratios from logistic regression analyses as estimates of the relative risk for an MPM based on the plasma concentrations in cases and controls

Article Snippet: Thrombomodulin concentration was determined according to the manufacturer´s instructions (DY3947, R&D Systems).

Techniques:

ROC curves of MPM biomarkers in incident cases and controls by gender (all CIs were 95%). A, Males: mesothelin (AUC=0.90, CI:0.85-0.95), calretinin (AUC=0.88, CI:0.82-0.94), and thrombomodulin (AUC=0.51, CI:0.41-0.61) B, Females: mesothelin (AUC=0.92, CI:0.79-1.00), calretinin (AUC=0.77, CI:0.61-0.93), and thrombomodulin (AUC=0.52, CI: 0.32-0.72).

Journal: International Journal of Medical Sciences

Article Title: Biomarkers for Predicting Malignant Pleural Mesothelioma in a Mexican Population

doi: 10.7150/ijms.23939

Figure Lengend Snippet: ROC curves of MPM biomarkers in incident cases and controls by gender (all CIs were 95%). A, Males: mesothelin (AUC=0.90, CI:0.85-0.95), calretinin (AUC=0.88, CI:0.82-0.94), and thrombomodulin (AUC=0.51, CI:0.41-0.61) B, Females: mesothelin (AUC=0.92, CI:0.79-1.00), calretinin (AUC=0.77, CI:0.61-0.93), and thrombomodulin (AUC=0.52, CI: 0.32-0.72).

Article Snippet: Thrombomodulin concentration was determined according to the manufacturer´s instructions (DY3947, R&D Systems).

Techniques: