Journal: Journal of cellular physiology

Article Title: Mitogen-Dependent Regulation of DUSP1 Governs ERK and p38 Signaling During Early 3T3-L1 Adipocyte Differentiation

doi: 10.1002/jcp.25248

Figure Lengend Snippet: DUSP and inflammatory genes analyzed in this study

Article Snippet: Dusp16 , MKP-7, MKP-M , III , ● , ● , ● , J/P , NM_130447 , Mm00459935_m1 , 25.

Techniques:

Journal: Cancer Cell International

Article Title: Exosomal transfer of miR-769-5p promotes osteosarcoma proliferation and metastasis by targeting DUSP16

doi: 10.1186/s12935-021-02257-4

Figure Lengend Snippet: DUSP16 is down-regulated in OS and is a downstream target gene of miR-769-5p. A Bioinformatics analysis showed that hsa-miR-769-5p has a total of 14 target genes in miRDB, miRTarBase, and TargetScan; B, C The mRNA expression level of DUSP16 in OS cell lines and hFOB1.19 ( B ) and clinical samples ( C ); D , E The protein level of DUSP16 in OS cell lines and hFOB1.19 ( D ) and clinical samples ( E ); F Representative immunohistochemical staining of DUSP16 between OS tissues and adjacent tissues; G Negative correlation between miR-769-5p and DUSP16 expression in OS tissues; H Luciferase reporter assay was performed to confirm that miR-769-5p directly bound to the 3′-UTR region of DUSP16. Luciferase activity was analyzed in OS cells co-transfected with miR-769-5p mimics or negative control with pGL3-DUSP16-WT or pGL3- DUSP16-MUT; I , J The expression of DUSP16 in OS cells after alteration of miR-769-5p expression was detected by qRT-PCR ( I ) and western blot ( J ); K , L Western blot ( K ) and immunohistochemical analysis ( L ) were performed to evaluate DUSP16 expression in vivo; n = 5 mice/group. Data are presented as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: GenScript (Nanjing, China) synthesized wild-type DUSP16 (WT-DUSP16-3 UTR) and mutant DUSP16 (MUT-DUSP16-3 UTR).

Techniques: Expressing, Immunohistochemical staining, Staining, Luciferase, Reporter Assay, Activity Assay, Transfection, Negative Control, Quantitative RT-PCR, Western Blot, In Vivo

Journal: Cancer Cell International

Article Title: Exosomal transfer of miR-769-5p promotes osteosarcoma proliferation and metastasis by targeting DUSP16

doi: 10.1186/s12935-021-02257-4

Figure Lengend Snippet: miR-769-5p promotes EMT and proliferation by suppressing DUSP16 in OS cells. A Western blot analysis was conducted to evaluate the EMT-related protein levels. Rescue experiments for miR-769-5p mimics were conducted via the ectopic expression of DUSP16 in MG63 cells. Rescue experiments for miR-769-5p inhibitor were conducted by downregulating DUSP16 in 143B cells; B-F Rescue experiments were conducted using the Scratch assay ( B , D ), transwell migration assay ( C , E ) and transwell invasion assay ( C , F ); G Western blot analysis was also conducted to evaluate the cell-cycle-related proteins level; H–K Rescue experiments were also conducted using the EdU assay ( H , I ), clone formation assay ( H , J ) and CCK-8 assay ( K ). Data are presented as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: GenScript (Nanjing, China) synthesized wild-type DUSP16 (WT-DUSP16-3 UTR) and mutant DUSP16 (MUT-DUSP16-3 UTR).

Techniques: Western Blot, Expressing, Wound Healing Assay, Transwell Migration Assay, Transwell Invasion Assay, EdU Assay, Tube Formation Assay, CCK-8 Assay

Journal: Cancer Cell International

Article Title: Exosomal transfer of miR-769-5p promotes osteosarcoma proliferation and metastasis by targeting DUSP16

doi: 10.1186/s12935-021-02257-4

Figure Lengend Snippet: miR-769-5p regulates the JNK/p38 MAPK signaling pathway by targeting DUSP16. A Representative images of western blot analysis of p-JNK, JNK, p-ERK, ERK, p-P38, P38 and DUSP16 levels in transfected MG63 and 143B cells; B Representative images of western blot analysis of p-JNK, JNK, N-cadherin, Vimentin, E-cadherin, c-Myc, CDK4 and cyclin D1 levels in transfected MG63 and 143B cells treated with the JNK inhibitor sp600125 and the JNK activator Anisomycin, respectively

Article Snippet: GenScript (Nanjing, China) synthesized wild-type DUSP16 (WT-DUSP16-3 UTR) and mutant DUSP16 (MUT-DUSP16-3 UTR).

Techniques: Western Blot, Transfection

Journal: Cancer Cell International

Article Title: Exosomal transfer of miR-769-5p promotes osteosarcoma proliferation and metastasis by targeting DUSP16

doi: 10.1186/s12935-021-02257-4

Figure Lengend Snippet: BMSC-derived exosomes can be taken up by OS cells. A Transmission electron micrograph of exosomes derived from BSMCs; B Nanoparticle tracking analysis of the diameter and concentration of exosomes; C Exosomal biomarkers protein were further examined by western blot; D Uptake of Dil-labelled exosomes by MG63 and 143B cells was detected; E , F The mRNA expression level of miR-769-5p ( E ) and DUSP16 ( F ) was detected by qRT-PCR in different groups; G The expression of DUSP16 protein was detected by western blotting in different groups. Data are presented as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: GenScript (Nanjing, China) synthesized wild-type DUSP16 (WT-DUSP16-3 UTR) and mutant DUSP16 (MUT-DUSP16-3 UTR).

Techniques: Derivative Assay, Transmission Assay, Concentration Assay, Western Blot, Expressing, Quantitative RT-PCR

Journal: Cancer Cell International

Article Title: Exosomal transfer of miR-769-5p promotes osteosarcoma proliferation and metastasis by targeting DUSP16

doi: 10.1186/s12935-021-02257-4

Figure Lengend Snippet: Identification of Exosomes in the Serum of Patients and Graphical Abstract. A Transmission electron micrographs of exosomes derived from OS patients and healthy volunteers; B The expression of miR-769-5p in serum exosomal of OS patients compared to volunteers; C miR-769-5p derived from BMSCs exosomes promotes OS cells migration, invasion and proliferation by downregulating DUSP16 and then activating the JNK/P38 MAPK signaling pathway. Data are presented as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: GenScript (Nanjing, China) synthesized wild-type DUSP16 (WT-DUSP16-3 UTR) and mutant DUSP16 (MUT-DUSP16-3 UTR).

Techniques: Transmission Assay, Derivative Assay, Expressing, Migration