Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: RDT types and brands verified with microscopy

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques:

Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: New Summary of results table

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques: Diagnostic Assay, Microscopy, Sampling

Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: Paracheck‐Pf.

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques:

Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: ParaHIT‐F.

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques:

Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: PCR adjusted microscopy, Type 1, Paracheck‐PF (All).

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques: Microscopy

Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: PCR, Type 1, ParaHIT‐F.

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques:

Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: Data tables by test

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques: Microscopy

Journal: PLoS ONE

Article Title: Impact of Whole-Genome and Tandem Duplications in the Expansion and Functional Diversification of the F-Box Family in Legumes (Fabaceae)

doi: 10.1371/journal.pone.0055127

Figure Lengend Snippet: The outer circle shows numbered chromosomes of each species in gray ( M. truncatula , Mt), light gray ( G. max , Gm) and dark gray ( V. vinifera , Vv). Local duplications are represented in the second outer circle, where red denotes higher density of tandem duplications in a particular region. The line plot illustrates the number of FBX genes in each interval of 100 genes. If 5 or more FBX genes are present in a given region, the peak is colored in red. Internal arcs connect syntenic regions between V. vinifera / G. max (blue) and V. vinifera / M. truncatula (green). Colored triangles represent tandemly-duplicated FBX genes with preferential expression in late-development seeds (green), late embryogenesis seeds (red), nodules (yellow). For Gm: no detectable transcription (gray), apical meristem (green), nodule (blue), flower (yellow) and leaves (purple).

Article Snippet: For example, while many Glycine max FBX content emerged from segmental duplications, Medicago truncatula shows a high prevalence of FBX gene duplications in tandem.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Impact of Whole-Genome and Tandem Duplications in the Expansion and Functional Diversification of the F-Box Family in Legumes (Fabaceae)

doi: 10.1371/journal.pone.0055127

Figure Lengend Snippet: Normalized transcriptional levels were obtained from Severin at al (A) and Libault et al (B) ( G. max ) and Benedito et al ( M. truncatula ) (C) . For each independent study, gene expression values were standardized using Z-score and clustered with Hierarchical Clustering (MeV package). Numbered labels in the right refer to tandem FBX arrays (i.e. if two genes have the same number, they are very close to each other in the genome). These labels are qualitative and thus there is no correlation between label number and genomic closeness of the tandem FBX arrays.

Article Snippet: For example, while many Glycine max FBX content emerged from segmental duplications, Medicago truncatula shows a high prevalence of FBX gene duplications in tandem.

Techniques: Gene Expression