dulbecco 's phosphate-buffered saline Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher phosphate buffered saline dulbecco s
    Pop#8 exclusive in vitro generation of MPCs. After 5 days in MPC-selective culture conditions (DMEM/10%PhABS) Pop#8 generated MPC-like cells characterized by the peculiar fried egg-shaped morphology (A) . CD3/20/14/CD66 + cells were sorted in parallel, as control population (B) . Immunofluorescence analysis of Pop#8 -derived MPCs, revealed podosome-like actin organization ( red in C) and intense positivity to nestin ( green in C) . The MPC phenotype was confirmed by flow cytometry (D) . After 7 days of culture under mesengenic-differentiating conditions (MesenPRO ® RS medium), Pop#8 -derived MPCs gave rise to confluent cells showing MSC phenotype (E) and morphology (F) . To confirm the mesenchymal nature of Pop#8 MPC-derived MSCs, cultures were further exposed to adipogenic or osteogenic stimuli. Lipid droplet ( red in G) accumulation and calcium deposition ( green in G ) were detected only in terminally differentiated cultures (G , upper panels ). No fluorescence signals were detected in cultures maintained in MesenPRO RS medium (G) ( lower panels ). DMEM, <t>Dulbecco's</t> modified Eagle's medium; PhABS, pooled human AB-type serum; RS, reduced serum.
    Phosphate Buffered Saline Dulbecco S, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline dulbecco s/product/Thermo Fisher
    Average 99 stars, based on 447 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline dulbecco s - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Millipore dulbecco s phosphatebuffered saline
    Pop#8 exclusive in vitro generation of MPCs. After 5 days in MPC-selective culture conditions (DMEM/10%PhABS) Pop#8 generated MPC-like cells characterized by the peculiar fried egg-shaped morphology (A) . CD3/20/14/CD66 + cells were sorted in parallel, as control population (B) . Immunofluorescence analysis of Pop#8 -derived MPCs, revealed podosome-like actin organization ( red in C) and intense positivity to nestin ( green in C) . The MPC phenotype was confirmed by flow cytometry (D) . After 7 days of culture under mesengenic-differentiating conditions (MesenPRO ® RS medium), Pop#8 -derived MPCs gave rise to confluent cells showing MSC phenotype (E) and morphology (F) . To confirm the mesenchymal nature of Pop#8 MPC-derived MSCs, cultures were further exposed to adipogenic or osteogenic stimuli. Lipid droplet ( red in G) accumulation and calcium deposition ( green in G ) were detected only in terminally differentiated cultures (G , upper panels ). No fluorescence signals were detected in cultures maintained in MesenPRO RS medium (G) ( lower panels ). DMEM, <t>Dulbecco's</t> modified Eagle's medium; PhABS, pooled human AB-type serum; RS, reduced serum.
    Dulbecco S Phosphatebuffered Saline, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s phosphatebuffered saline/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    dulbecco s phosphatebuffered saline - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher d pbs
    Zinc acetate in HEC does not prevent vaginal or rectal HSV-2 infection in mice. <t>Medroxyprogesterone</t> acetate-treated (vaginal; A) or untreated (rectal; B) mice had the indicated gels (versus <t>D-PBS</t> or the zinc acetate solution) applied vaginally or rectally 10 min before being challenged with 10 6 PFU (via the respective routes). Survival was monitored for up to 20 days, and the survival curves are shown for each condition ( n = 15 to 20 for each treatment group).
    D Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d pbs/product/Thermo Fisher
    Average 99 stars, based on 2650 article reviews
    Price from $9.99 to $1999.99
    d pbs - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher d pbs buffer
    Zinc acetate in HEC does not prevent vaginal or rectal HSV-2 infection in mice. <t>Medroxyprogesterone</t> acetate-treated (vaginal; A) or untreated (rectal; B) mice had the indicated gels (versus <t>D-PBS</t> or the zinc acetate solution) applied vaginally or rectally 10 min before being challenged with 10 6 PFU (via the respective routes). Survival was monitored for up to 20 days, and the survival curves are shown for each condition ( n = 15 to 20 for each treatment group).
    D Pbs Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d pbs buffer/product/Thermo Fisher
    Average 88 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    d pbs buffer - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    Image Search Results


    Pop#8 exclusive in vitro generation of MPCs. After 5 days in MPC-selective culture conditions (DMEM/10%PhABS) Pop#8 generated MPC-like cells characterized by the peculiar fried egg-shaped morphology (A) . CD3/20/14/CD66 + cells were sorted in parallel, as control population (B) . Immunofluorescence analysis of Pop#8 -derived MPCs, revealed podosome-like actin organization ( red in C) and intense positivity to nestin ( green in C) . The MPC phenotype was confirmed by flow cytometry (D) . After 7 days of culture under mesengenic-differentiating conditions (MesenPRO ® RS medium), Pop#8 -derived MPCs gave rise to confluent cells showing MSC phenotype (E) and morphology (F) . To confirm the mesenchymal nature of Pop#8 MPC-derived MSCs, cultures were further exposed to adipogenic or osteogenic stimuli. Lipid droplet ( red in G) accumulation and calcium deposition ( green in G ) were detected only in terminally differentiated cultures (G , upper panels ). No fluorescence signals were detected in cultures maintained in MesenPRO RS medium (G) ( lower panels ). DMEM, Dulbecco's modified Eagle's medium; PhABS, pooled human AB-type serum; RS, reduced serum.

    Journal: Stem Cells and Development

    Article Title: Mesangiogenic Progenitor Cells Derived from One Novel CD64brightCD31brightCD14neg Population in Human Adult Bone Marrow

    doi: 10.1089/scd.2015.0344

    Figure Lengend Snippet: Pop#8 exclusive in vitro generation of MPCs. After 5 days in MPC-selective culture conditions (DMEM/10%PhABS) Pop#8 generated MPC-like cells characterized by the peculiar fried egg-shaped morphology (A) . CD3/20/14/CD66 + cells were sorted in parallel, as control population (B) . Immunofluorescence analysis of Pop#8 -derived MPCs, revealed podosome-like actin organization ( red in C) and intense positivity to nestin ( green in C) . The MPC phenotype was confirmed by flow cytometry (D) . After 7 days of culture under mesengenic-differentiating conditions (MesenPRO ® RS medium), Pop#8 -derived MPCs gave rise to confluent cells showing MSC phenotype (E) and morphology (F) . To confirm the mesenchymal nature of Pop#8 MPC-derived MSCs, cultures were further exposed to adipogenic or osteogenic stimuli. Lipid droplet ( red in G) accumulation and calcium deposition ( green in G ) were detected only in terminally differentiated cultures (G , upper panels ). No fluorescence signals were detected in cultures maintained in MesenPRO RS medium (G) ( lower panels ). DMEM, Dulbecco's modified Eagle's medium; PhABS, pooled human AB-type serum; RS, reduced serum.

    Article Snippet: Isolation, fractioning, and plating of hBM mononuclear cells Fresh bone marrow samples were diluted 1:4 in Dulbecco's modified phosphate-buffered saline (D-PBS; Life Technologies, Carlsbad, CA) and gently layered on Ficoll-Paque™ PREMIUM (GE Healthcare, Uppsala, Sweden).

    Techniques: In Vitro, Generated, Immunofluorescence, Derivative Assay, Flow Cytometry, Cytometry, Fluorescence, Modification

    Specific motif for inhibition of elastolysis predicted for fibroblast HS and epithelial HS. ( A ) Chemical structure of the disaccharide pair that defines the cluster domain. Disaccharides excluded from the cluster domain belong to the connector domain. ( B ) Schematic diagram of HS chains showing domain organization with specific motif for inhibition of elastolysis. Yellow blocks are cluster domains; blue blocks are connector domains; dark blue blocks are connector domains that meet the size requirement for effective elastase inhibition. Chain lengths are 250 disaccharides. Minimum block length is 1 disaccharide. ( C ) Size distribution of connector domains in HS chains. N = 250 disaccharides and M = 100 chains. Crosshatched bars indicate lengths of connector domains (2–20 disaccharides) for effective elastase inhibition. Although the distribution is shown for connector domains up to 100 disaccharides in length, longer domains are present in both sets of HS chains. For fibroblast HS, connector domains extend to 226 disaccharides with an average size of 52±5 disaccharides (average ±95% confidence limits). For epithelial HS, connector domains extend to 184 disaccharides with an average size of 36±3 disaccharides. ( D ) Inhibition of elastolysis by GAG preparations. Relative rate = (elastin digestion with inhibitor)/(elastin digestion without inhibitor). Bar height equals the average of duplicate readings; error bar shows the propagation-of-error estimate using standard errors. Control = no inhibitor; Hep = commercial heparin (17–19 kDa); HS = commercial heparan sulfate (8–10 kDa); Fibro HS = HS preparation from rat pulmonary fibroblasts; Epi HS = HS preparation from rat pulmonary epithelial cells. Reaction conditions: [Inhibitor] = 5.0 µg/mL; [HNE (human neutrophil elastase)] = 120 nM; [Elastin] = 0.93 mg/mL; buffer = Dulbecco's phosphate-buffered saline without calcium and magnesium salts; temperature = 37°C; time = 4 hours; volume = 1.073 mL.

    Journal: PLoS ONE

    Article Title: A Computational Approach for Deciphering the Organization of Glycosaminoglycans

    doi: 10.1371/journal.pone.0009389

    Figure Lengend Snippet: Specific motif for inhibition of elastolysis predicted for fibroblast HS and epithelial HS. ( A ) Chemical structure of the disaccharide pair that defines the cluster domain. Disaccharides excluded from the cluster domain belong to the connector domain. ( B ) Schematic diagram of HS chains showing domain organization with specific motif for inhibition of elastolysis. Yellow blocks are cluster domains; blue blocks are connector domains; dark blue blocks are connector domains that meet the size requirement for effective elastase inhibition. Chain lengths are 250 disaccharides. Minimum block length is 1 disaccharide. ( C ) Size distribution of connector domains in HS chains. N = 250 disaccharides and M = 100 chains. Crosshatched bars indicate lengths of connector domains (2–20 disaccharides) for effective elastase inhibition. Although the distribution is shown for connector domains up to 100 disaccharides in length, longer domains are present in both sets of HS chains. For fibroblast HS, connector domains extend to 226 disaccharides with an average size of 52±5 disaccharides (average ±95% confidence limits). For epithelial HS, connector domains extend to 184 disaccharides with an average size of 36±3 disaccharides. ( D ) Inhibition of elastolysis by GAG preparations. Relative rate = (elastin digestion with inhibitor)/(elastin digestion without inhibitor). Bar height equals the average of duplicate readings; error bar shows the propagation-of-error estimate using standard errors. Control = no inhibitor; Hep = commercial heparin (17–19 kDa); HS = commercial heparan sulfate (8–10 kDa); Fibro HS = HS preparation from rat pulmonary fibroblasts; Epi HS = HS preparation from rat pulmonary epithelial cells. Reaction conditions: [Inhibitor] = 5.0 µg/mL; [HNE (human neutrophil elastase)] = 120 nM; [Elastin] = 0.93 mg/mL; buffer = Dulbecco's phosphate-buffered saline without calcium and magnesium salts; temperature = 37°C; time = 4 hours; volume = 1.073 mL.

    Article Snippet: Dulbecco's phosphate-buffered saline (PBS) without calcium and magnesium salts was ordered from Invitrogen (Carlsbad, CA).

    Techniques: Inhibition, Blocking Assay, Relative Rate

    Conversion of iPS cells into neuronal lineages in adherent monoculture. (A) FoxO3a -wild type and -null iPS cells were plated onto 0.1% gelatin-coated tissue culture plastic in Dulbecco's modified Eagle's medium/F12 with N2 and B27. At 0, 6, and 12 days,

    Journal: Stem Cells and Development

    Article Title: FoxO3a Contributes to the Reprogramming Process and the Differentiation of Induced Pluripotent Stem Cells

    doi: 10.1089/scd.2013.0044

    Figure Lengend Snippet: Conversion of iPS cells into neuronal lineages in adherent monoculture. (A) FoxO3a -wild type and -null iPS cells were plated onto 0.1% gelatin-coated tissue culture plastic in Dulbecco's modified Eagle's medium/F12 with N2 and B27. At 0, 6, and 12 days,

    Article Snippet: The head and visceral tissues were removed and the remaining bodies were washed in sterile Dulbecco's phosphate buffered saline (Invitrogen) and treated with 0.25% trypsin/1 mM ethylenediaminetetraacetic acid solution (Sigma-Aldrich).

    Techniques: Modification

    Schematic illustration of coprecipitating LN within biomimetic apatite. Abbreviations: LN, laminin; mDPBS, modified Dulbecco’s phosphate-buffered saline.

    Journal: International Journal of Nanomedicine

    Article Title: Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells

    doi: 10.2147/IJN.S176596

    Figure Lengend Snippet: Schematic illustration of coprecipitating LN within biomimetic apatite. Abbreviations: LN, laminin; mDPBS, modified Dulbecco’s phosphate-buffered saline.

    Article Snippet: Materials Dulbecco’s phosphate-buffered saline (DPBS, without calcium and magnesium), phosphate-buffered saline (PBS), accutase cell dissociation reagent, micro BCA protein assay kit (mBCA), serum albumin and live/dead viability/cytotoxicity kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Modification

    Zinc acetate in HEC does not prevent vaginal or rectal HSV-2 infection in mice. Medroxyprogesterone acetate-treated (vaginal; A) or untreated (rectal; B) mice had the indicated gels (versus D-PBS or the zinc acetate solution) applied vaginally or rectally 10 min before being challenged with 10 6 PFU (via the respective routes). Survival was monitored for up to 20 days, and the survival curves are shown for each condition ( n = 15 to 20 for each treatment group).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Zinc Acetate/Carrageenan Gels Exhibit Potent Activity In Vivo against High-Dose Herpes Simplex Virus 2 Vaginal and Rectal Challenge

    doi: 10.1128/AAC.05461-11

    Figure Lengend Snippet: Zinc acetate in HEC does not prevent vaginal or rectal HSV-2 infection in mice. Medroxyprogesterone acetate-treated (vaginal; A) or untreated (rectal; B) mice had the indicated gels (versus D-PBS or the zinc acetate solution) applied vaginally or rectally 10 min before being challenged with 10 6 PFU (via the respective routes). Survival was monitored for up to 20 days, and the survival curves are shown for each condition ( n = 15 to 20 for each treatment group).

    Article Snippet: Mice received subcutaneously 100 μl of medroxyprogesterone acetate (Depo-Provera; Upjohn, Kalamazoo, MI) at 25 mg/ml in D-PBS (Invitrogen).

    Techniques: Infection, Mouse Assay

    Repeated treatment with zinc-carrageenan formulations does not increase HSV-2 susceptibility in the mouse model. The indicated gel formulations were delivered intravaginally daily for 7 days to medroxyprogesterone acetate-treated BALB/c mice ( n = 20 to 24 per group). Twelve hours after the last gel was applied, mice were challenged with 2 × 10 3 PFU of HSV-2 strain G. Mice were examined and scored daily for 19 days. Percent survival over time is shown for each treatment group. There was not a significant difference between each individual gel (D, E, or G; P > 0.096) or the combination of the data from those three gels ( P > 0.055) compared to D-PBS or carrageenan gel. A very significant difference ( P = 0.0009 or P = 0.0004) was observed when comparing Gynol versus D-PBS or carrageenan, respectively.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Zinc Acetate/Carrageenan Gels Exhibit Potent Activity In Vivo against High-Dose Herpes Simplex Virus 2 Vaginal and Rectal Challenge

    doi: 10.1128/AAC.05461-11

    Figure Lengend Snippet: Repeated treatment with zinc-carrageenan formulations does not increase HSV-2 susceptibility in the mouse model. The indicated gel formulations were delivered intravaginally daily for 7 days to medroxyprogesterone acetate-treated BALB/c mice ( n = 20 to 24 per group). Twelve hours after the last gel was applied, mice were challenged with 2 × 10 3 PFU of HSV-2 strain G. Mice were examined and scored daily for 19 days. Percent survival over time is shown for each treatment group. There was not a significant difference between each individual gel (D, E, or G; P > 0.096) or the combination of the data from those three gels ( P > 0.055) compared to D-PBS or carrageenan gel. A very significant difference ( P = 0.0009 or P = 0.0004) was observed when comparing Gynol versus D-PBS or carrageenan, respectively.

    Article Snippet: Mice received subcutaneously 100 μl of medroxyprogesterone acetate (Depo-Provera; Upjohn, Kalamazoo, MI) at 25 mg/ml in D-PBS (Invitrogen).

    Techniques: Mouse Assay

    Zinc-carrageenan formulations do not negatively impact the architecture of cervicovaginal and rectal epithelia in vivo . Eight-week-old female BALB/c mice were treated with medroxyprogesterone acetate or fasted and anesthetized before adding D-PBS, carrageenan-based gel, formulation D, formulation G, or Gynol II to evaluate the normal architecture in the cervicovaginal and rectal mucosae. Mice were sacrificed at 1, 6, and 24 h after gel applications, and the entire reproductive or rectal tract was surgically excised, fixed, and embedded in paraffin before preparing tissue sections for morphological analyses using H E staining. Panel A (cervicovaginal mucosa) and panel B (rectal mucosa) represent a sample of the 6 sections of two or three animals that were analyzed per formulation. The original magnification is 20×, and the bar length represents 0.1 mm.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Zinc Acetate/Carrageenan Gels Exhibit Potent Activity In Vivo against High-Dose Herpes Simplex Virus 2 Vaginal and Rectal Challenge

    doi: 10.1128/AAC.05461-11

    Figure Lengend Snippet: Zinc-carrageenan formulations do not negatively impact the architecture of cervicovaginal and rectal epithelia in vivo . Eight-week-old female BALB/c mice were treated with medroxyprogesterone acetate or fasted and anesthetized before adding D-PBS, carrageenan-based gel, formulation D, formulation G, or Gynol II to evaluate the normal architecture in the cervicovaginal and rectal mucosae. Mice were sacrificed at 1, 6, and 24 h after gel applications, and the entire reproductive or rectal tract was surgically excised, fixed, and embedded in paraffin before preparing tissue sections for morphological analyses using H E staining. Panel A (cervicovaginal mucosa) and panel B (rectal mucosa) represent a sample of the 6 sections of two or three animals that were analyzed per formulation. The original magnification is 20×, and the bar length represents 0.1 mm.

    Article Snippet: Mice received subcutaneously 100 μl of medroxyprogesterone acetate (Depo-Provera; Upjohn, Kalamazoo, MI) at 25 mg/ml in D-PBS (Invitrogen).

    Techniques: In Vivo, Mouse Assay, Staining