ATCC
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dulbecco s modified eagle medium dmem Dulbecco S Modified Eagle Medium Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 21777 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/dulbecco s modified eagle medium dmem/product/Thermo Fisher Average 99 stars, based on 21777 article reviews Price from $9.99 to $1999.99
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Millipore
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Thermo Fisher
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Thermo Fisher
dulbecco s modified eagle s medium f12 ![]() Dulbecco S Modified Eagle S Medium F12, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2341 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/dulbecco s modified eagle s medium f12/product/Thermo Fisher Average 99 stars, based on 2341 article reviews Price from $9.99 to $1999.99
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ATCC
dulbecco s modified eagle s medium ![]() Dulbecco S Modified Eagle S Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1590 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/dulbecco s modified eagle s medium/product/ATCC Average 94 stars, based on 1590 article reviews Price from $9.99 to $1999.99
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dulbecco s modified eagle s medium ![]() Dulbecco S Modified Eagle S Medium, supplied by Welgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/dulbecco s modified eagle s medium/product/Welgene inc Average 92 stars, based on 1407 article reviews Price from $9.99 to $1999.99
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Optimized for use with embryonic stem ES cell cultures Each lot is qualified to support ES cell growth Contains 4500 mg L glucose sodium pyruvate and sodium bicarbonate Formulated for
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Image Search Results

Journal: Frontiers in Microbiology
Article Title: Identification of Chebulinic Acid and Chebulagic Acid as Novel Influenza Viral Neuraminidase Inhibitors
doi: 10.3389/fmicb.2020.00182
Figure Lengend Snippet: Reporter luciferase-based one-cycle infection inhibition assay. MDCK cells growing in 96-well plates were infected with reporter influenza PR8-PB2-Gluc virus at an MOI of 0.1, in the absence or presence of increasing concentrations of (A) CHLA and (B) CHLI. Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 4% fetal bovine serum (FBS) were used as culture medium during infection to avoid generation of infectious progeny viruses. At 24 h post infection (p.i.), Gluc expression was determined, and viral infectivities were evaluated. Data are means ± SD from three independent experiments. * p
Article Snippet: Cells, Viruses, and Compounds Madin–Darby canine kidney (MDCK) epithelial cells were grown in
Techniques: Luciferase, Infection, Inhibition, Modification, Expressing

Journal: International Journal of Oncology
Article Title: 2,3,7,8-tetrachlorodibenzo-p-dioxin suppresses the growth of human colorectal cancer cells in vitro: Implication of the aryl hydrocarbon receptor signaling
doi: 10.3892/ijo.2019.4703
Figure Lengend Snippet: TCDD suppresses colony formation in RKO human colorectal cancer cells in vitro . Cells (1×10 3 cells/well) were seeded into 6-well plates and cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, 1% penicillin/streptomycin and 1% fungizone in the presence of vehicle (1% dimethyl sulfoxide) or TCDD (1 or 10 nM) for 5 days when visible clones formed. The colonies were washed with PBS, fixed with methanol and then stained with 0.5% crystal violet. (A) Stained cells are presented as images (×10), and (B) the colonies containing > 50 cells were counted under a microscope. * P
Article Snippet:
Techniques: In Vitro, Cell Culture, Modification, Clone Assay, Staining, Microscopy

Journal: International Journal of Oncology
Article Title: 2,3,7,8-tetrachlorodibenzo-p-dioxin suppresses the growth of human colorectal cancer cells in vitro: Implication of the aryl hydrocarbon receptor signaling
doi: 10.3892/ijo.2019.4703
Figure Lengend Snippet: TCDD suppresses the proliferation of RKO human colorectal cancer cells in vitro . The cells (1×10 5 cells/well in 24-well plates) were cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, 1% penicillin/streptomycin and 1% fungizone in the presence of vehicle (1% dimethyl sulfoxide) or TCDD (0.01-100 nM) for (A) 3 or (B) 7 days. After culture, the numbers of attached cells were counted. Data are presented as mean ± standard deviation obtained from 8 wells of 2 replicate plates per dataset using different dishes and cell preparations. * P
Article Snippet:
Techniques: In Vitro, Cell Culture, Modification, Standard Deviation

Journal: International Journal of Oncology
Article Title: 2,3,7,8-tetrachlorodibenzo-p-dioxin suppresses the growth of human colorectal cancer cells in vitro: Implication of the aryl hydrocarbon receptor signaling
doi: 10.3892/ijo.2019.4703
Figure Lengend Snippet: The TCDD-induced increase in CYP1A1 levels are suppressed in the regucalcin-overexpressing RKO human colorectal cancer cells in vitro . The wild-type RKO cells or regucalcin-overexpressing transfectants (1×10 6 cells/dish) were cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, 1% penicillin/streptomycin and 1% fungizone in the presence or absence of vehicle (1% dimethyl sulfoxide) or TCDD (10 nM) for 3 days and then cell lysates were centrifuged. Subsequently, 40 µ g of the supernatant protein per lane were separated by SDS-PAGE and transferred to nylon membranes for western blotting using specific antibodies against the indicated proteins. Representative data from three independent experiments using different cell preparations are presented, and data are presented as mean ± standard deviation. (A) Representative film images of the TCDD effect. (B) Presented relative to β-actin of the TCDD effect. * P
Article Snippet:
Techniques: In Vitro, Cell Culture, Modification, SDS Page, Western Blot, Standard Deviation

Journal: International Journal of Oncology
Article Title: 2,3,7,8-tetrachlorodibenzo-p-dioxin suppresses the growth of human colorectal cancer cells in vitro: Implication of the aryl hydrocarbon receptor signaling
doi: 10.3892/ijo.2019.4703
Figure Lengend Snippet: TCDD regulates the expression of proteins associated with AHR signaling in RKO human colorectal cancer cells in vitro . The cells (1×10 6 cells/dish) were cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, 1% penicillin/streptomycin and 1% fungizone in the presence of vehicle (1% dimethyl sulfoxide) or TCDD (10 nM) for 3 days. Cell lysates were prepared and centrifuged, and 40 µ g of the supernatant protein per lane were separated by SDS-PAGE and transferred to nylon membranes for western blotting using specific antibodies against various proteins as indicated. Data represent a typical figure of three independent experiments using different cell preparations, and also are presented as mean ± standard deviation. (A) Representative film image for cell signaling-associated proteins. (B) Relative to β-actin cell signaling-associated protein levels. (C) Representative film image of tumor suppressor proteins.(D) Relative to β-actin tumor suppressor proteins. * P
Article Snippet:
Techniques: Expressing, In Vitro, Cell Culture, Modification, SDS Page, Western Blot, Standard Deviation

Journal: International Journal of Oncology
Article Title: 2,3,7,8-tetrachlorodibenzo-p-dioxin suppresses the growth of human colorectal cancer cells in vitro: Implication of the aryl hydrocarbon receptor signaling
doi: 10.3892/ijo.2019.4703
Figure Lengend Snippet: AHR and CYP1A1 levels are suppressed in regucalcin-overexpressing RKO human colorectal cancer cells in vitro . The wild-type RKO cells or regucalcin-overexpressing RKO cells (1×10 6 cells/per dish) were cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, 1% penicillin/streptomycin and 1% fungizone in the presence or absence of vehicle (1% dimethyl sulfoxide) for 3 days. After culture, resulting cell lysates were centrifuged, and 40 µ g of the supernatant protein per lane were separated by SDS-PAGE and transferred to nylon membranes for western blotting using specific antibodies as indicated. Data represent a typical figure obtained from three independent experiments using different cell preparations, and also are presented as mean ± standard deviation. (A) Representative film image for regucalcin. (B) Relative to β-actin regucalcin level. (C) Representative film image of AHR and CYP1A1. (D) Relative to β-actin AHR and CYP1A1 levels. * P
Article Snippet:
Techniques: In Vitro, Cell Culture, Modification, SDS Page, Western Blot, Standard Deviation

Journal: Drug Design, Development and Therapy
Article Title: (+)-Grandifloracin, an antiausterity agent, induces autophagic PANC-1 pancreatic cancer cell death
doi: 10.2147/DDDT.S52168
Figure Lengend Snippet: Effect of conventional anticancer agents against PANC-1 cells after 24 hours in NDM and DMEM. Data are expressed as the mean ± standard deviation, n=3. Abbreviations: NDM, nutrient-deprived medium; DMEM, Dulbecco’s Modified Eagle’s Medium; 5-FU, 5-fluorouracil; 2-DG, 2-deoxyglucose.
Article Snippet:
Techniques: Standard Deviation, Modification

Journal: Drug Design, Development and Therapy
Article Title: (+)-Grandifloracin, an antiausterity agent, induces autophagic PANC-1 pancreatic cancer cell death
doi: 10.2147/DDDT.S52168
Figure Lengend Snippet: Assessment of cytotoxicity of conventional anticancer agents against PANC-1 cells in Dulbecco’s Modified Eagle’s Medium. Data are expressed as the mean ± standard deviation, n=3. * P
Article Snippet:
Techniques: Modification, Standard Deviation

Journal: Drug Design, Development and Therapy
Article Title: (+)-Grandifloracin, an antiausterity agent, induces autophagic PANC-1 pancreatic cancer cell death
doi: 10.2147/DDDT.S52168
Figure Lengend Snippet: Effect of GF against Akt, mTOR, LC3A/B I, and LC3A/B II. Abbreviations: GF, (+)-grandifloracin; NDM, nutrient-deprived medium; DMEM, Dulbecco’s Modified Eagle’s Medium.
Article Snippet:
Techniques: Modification

Journal: Biomolecules
Article Title: High Levels of ROS Impair Lysosomal Acidity and Autophagy Flux in Glucose-Deprived Fibroblasts by Activating ATM and Erk Pathways
doi: 10.3390/biom10050761
Figure Lengend Snippet: Autophagy flux is impaired in glucose-deprived fibroblasts. ( A ) Human fibroblasts on cover slips were cultivated in normal (Glu(+)) or glucose-free Dulbecco’s modified Eagle’s medium (DMEM) (Glu(-)) for the indicated periods and processed for immunostaining for the LC3 protein (red). Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Cells were examined by confocal microscopy. ( B ) Cells cultivated in glucose-free medium were examined by immunoblotting analysis for p62 sequestosome 1 (p62). ( C ) Change in LC3 protein level was determined by immunoblotting in cells cultivated in the absence of glucose for indicated times. The cells were either mock-treated or treated with 200-nM bafilomycin A1 (bafA1) or 50 μM chloroquine (CQ) for 6 h prior to collection for immunoblotting. Relative autophagic flux is shown in the bar graphs (below) of LC-II proteins levels quantitated by densitometry. The LC3-II level in the cells treated with bafA1 or CQ (+) was normalized by that in the cells untreated (-), and the ratio (R) is presented as an indication of the relative flux. ( D ) Cells transfected with plasmid that expresses tandem fluorescence LC3 (tfLC3) were cultivated in the absence of glucose for indicated periods or treated with 250-nM torin1 for 24 h. After fixing, tfLC3 fluorescence was visualized by confocal microscopy. In the right-most column, merged images (yellow) of the green and red fluorescence visualizes structures emitting both green and red fluorescence of GFP and RFP. All scale bars in microscopy indicate 5 μM.
Article Snippet: Cell Culture and Chemical Treatments Normal human fibroblasts isolated from healthy newborn foreskins and provided by Dr. Jin-Ho Chung (IRB No. H-1101-116-353 of the School of Medicine, Seoul National University, Korea) were maintained in
Techniques: Modification, Immunostaining, Staining, Confocal Microscopy, Transfection, Plasmid Preparation, Fluorescence, Microscopy