Journal: Scientific Reports

Article Title: Styrene maleic acid recovers proteins from mammalian cells and tissues while avoiding significant cell death

doi: 10.1038/s41598-019-51896-1

Figure Lengend Snippet: Low-dose styrene maleic acid application effectively samples proteins from cells. ( a ) Labelling of cells with biotin prior to 6.25 ppm SMA application allowed confirmation with biotin immunoblotting of the presence of a range of proteins (20–200 kDa) in the obtained suspension. ( b ) Proteins were absent from the final post-biotinylation Dulbecco’s PBS cell wash, as confirmed across repetitions (ANOVA plus Tukey’s test * p

Article Snippet: Cell culture Both CFs and VSMCs were plated and maintained in the following medium: Dulbecco’s Modified Eagles’ Medium (DMEM, cat. no. 21969); 10% Foetal Calf Serum (FCS); 1% Glutamax; 1% Penicillin-Streptomycin; 0.1% Fungizone (all Life Technologies; percentages v/v).

Techniques:

Journal: British Journal of Cancer

Article Title: Cell migration in paediatric glioma; characterisation and potential therapeutic targeting

doi: 10.1038/bjc.2015.16

Figure Lengend Snippet: Inhibition of paediatric glioma tumour sphere invasion by LiCl and BIO. Tumour spheroids (SF188) were embedded in collagen and incubated in ( A ) LiCl at 40, 20, 10, 5 m M in NaCl or ( B ) BIO at 10, 5, 1, 0.5 μ M in DMSO for 72 h. Migration was inhibited by both LiCl and BIO as shown in representative stills at 24, 48 and 72 h using the EVOS cell imaging system at × 40 magnification. Scale bar=1000 μ m. ( C ) Quantitative analysis of migration inhibition confirmed the results visualised by microscopy. Control medium used was Dulbecco's Modified Eagles' medium plus 10% heat-inactivated foetal calf serum. Both LiCl and BIO had statistically significant anti-migratory effects on the invasion zone and leading edge as indicated by * on all three cell lines (SF188, KNS42 and HSJD-DIPG-007). Results are representative of n =3 individual experiments. Error bars expressed as ±s.e.m. * P

Article Snippet: Cells were grown in Dulbecco's Modified Eagles' medium (Sigma-Aldrich, Dorset, UK) with 10% heat-inactivated foetal calf serum (Labtech, East Sussex, UK) and 0.5% penicillin-streptomycin (Sigma-Aldrich).

Techniques: Inhibition, Incubation, Migration, Imaging, Microscopy, Modification

Journal: International Journal of Molecular Sciences

Article Title: Catabolism of Fibromodulin in Developmental Rudiment and Pathologic Articular Cartilage Demonstrates Novel Roles for MMP-13 and ADAMTS-4 in C-terminal Processing of SLRPs

doi: 10.3390/ijms20030579

Figure Lengend Snippet: Glycosaminoglycan (GAG; A ) and collagen content ( B ) of ovine articular cartilage explants after 5 or 12 days of culture in Dulbecco’s Modified Eagles Medium (DMEM) without (control) or with 5 ng/mL IL-1 and 50 ng/mL oncostatin M (IL-1/OSM) ± 300 nM MMP-inhibitor PGE3162689 (expressed as % of the total in explant plus media; mean ± standard deviation) ( n = 6). Significant differences between control and treated cultures were defined as * p

Article Snippet: Ovine Cartilage Explant Cultures Full-depth articular cartilage explants were harvested from the trochlear groove of 6–12-month-old ovine knee joints and cultured at 37 °C, 5% (v /v ) CO2 for 48 h in Dulbecco’s Modified Eagles Medium (DMEM; Sigma, Castle Hill, NSW, Australia), buffered with sodium bicarbonate 3.7 g/L (Fronine, Riverstone, NSW, Australia) and containing 10% (v /v ) Fetal Calf Serum (FCS; Trace Biosciences Pty.

Techniques: Modification, Standard Deviation