Journal: British Journal of Cancer
Article Title: Cell migration in paediatric glioma; characterisation and potential therapeutic targeting
Figure Lengend Snippet: Inhibition of paediatric glioma tumour sphere invasion by LiCl and BIO. Tumour spheroids (SF188) were embedded in collagen and incubated in ( A ) LiCl at 40, 20, 10, 5 m M in NaCl or ( B ) BIO at 10, 5, 1, 0.5 μ M in DMSO for 72 h. Migration was inhibited by both LiCl and BIO as shown in representative stills at 24, 48 and 72 h using the EVOS cell imaging system at × 40 magnification. Scale bar=1000 μ m. ( C ) Quantitative analysis of migration inhibition confirmed the results visualised by microscopy. Control medium used was Dulbecco's Modified Eagles' medium plus 10% heat-inactivated foetal calf serum. Both LiCl and BIO had statistically significant anti-migratory effects on the invasion zone and leading edge as indicated by * on all three cell lines (SF188, KNS42 and HSJD-DIPG-007). Results are representative of n =3 individual experiments. Error bars expressed as ±s.e.m. * P
Article Snippet: Cells were grown in Dulbecco's Modified Eagles' medium (Sigma-Aldrich, Dorset, UK) with 10% heat-inactivated foetal calf serum (Labtech, East Sussex, UK) and 0.5% penicillin-streptomycin (Sigma-Aldrich).
Techniques: Inhibition, Incubation, Migration, Imaging, Microscopy, Modification