Journal:

Article Title: Corticotropin-releasing hormone triggers differentiation in HaCaT keratinocytes

doi: 10.1111/J.1365-2133.2005.06217.X

Figure Lengend Snippet: Corticotropin-releasing hormone (CRH) increases accumulation of cells in the G0/1 phase of the cell cycle. Cells were treated with 1 nmol L −1 CRH for 24 h in serum-free high-calcium Dulbecco’s modified Eagles’s medium (A) or with

Article Snippet: Dulbecco’s modified Eagles’s medium (DMEM), fetal calf serum (FCS), 0·25% trypsin/1 mmol L−1 ethylenediamine tetraacetic acid solution, phosphate buffered saline (PBS) and solution containing penicillin G, streptomycin and amphotericin B were purchased from Gibco, Invitrogen Life Technnologies (Carlsbad, CA, U.S.A).

Techniques: Modification

Journal:

Article Title: Corticotropin-releasing hormone triggers differentiation in HaCaT keratinocytes

doi: 10.1111/J.1365-2133.2005.06217.X

Figure Lengend Snippet: Corticotropin-releasing hormone (CRH) increases AP-1 binding activity in HaCaT cells. HaCaT cells were stimulated with 100 nmol L −1 CRH for 60 min in serum-free Dulbecco’s modified Eagles’s medium. Nuclear extracts were subjected

Article Snippet: Dulbecco’s modified Eagles’s medium (DMEM), fetal calf serum (FCS), 0·25% trypsin/1 mmol L−1 ethylenediamine tetraacetic acid solution, phosphate buffered saline (PBS) and solution containing penicillin G, streptomycin and amphotericin B were purchased from Gibco, Invitrogen Life Technnologies (Carlsbad, CA, U.S.A).

Techniques: Binding Assay, Activity Assay, Modification

Journal:

Article Title: Corticotropin-releasing hormone triggers differentiation in HaCaT keratinocytes

doi: 10.1111/J.1365-2133.2005.06217.X

Figure Lengend Snippet: Corticotropin-releasing hormone (CRH) triggers an early differentiation programme in HaCaT keratinocytes. HaCaT cells were stimulated with for 24 h in serum-free high-calcium Dulbecco’s modified Eagles’s medium (A, B) or for 48 h in supplement-free

Article Snippet: Dulbecco’s modified Eagles’s medium (DMEM), fetal calf serum (FCS), 0·25% trypsin/1 mmol L−1 ethylenediamine tetraacetic acid solution, phosphate buffered saline (PBS) and solution containing penicillin G, streptomycin and amphotericin B were purchased from Gibco, Invitrogen Life Technnologies (Carlsbad, CA, U.S.A).

Techniques: Modification

Journal: British Journal of Cancer

Article Title: Cell migration in paediatric glioma; characterisation and potential therapeutic targeting

doi: 10.1038/bjc.2015.16

Figure Lengend Snippet: Inhibition of paediatric glioma tumour sphere invasion by LiCl and BIO. Tumour spheroids (SF188) were embedded in collagen and incubated in ( A ) LiCl at 40, 20, 10, 5 m M in NaCl or ( B ) BIO at 10, 5, 1, 0.5 μ M in DMSO for 72 h. Migration was inhibited by both LiCl and BIO as shown in representative stills at 24, 48 and 72 h using the EVOS cell imaging system at × 40 magnification. Scale bar=1000 μ m. ( C ) Quantitative analysis of migration inhibition confirmed the results visualised by microscopy. Control medium used was Dulbecco's Modified Eagles' medium plus 10% heat-inactivated foetal calf serum. Both LiCl and BIO had statistically significant anti-migratory effects on the invasion zone and leading edge as indicated by * on all three cell lines (SF188, KNS42 and HSJD-DIPG-007). Results are representative of n =3 individual experiments. Error bars expressed as ±s.e.m. * P

Article Snippet: Cells were grown in Dulbecco's Modified Eagles' medium (Sigma-Aldrich, Dorset, UK) with 10% heat-inactivated foetal calf serum (Labtech, East Sussex, UK) and 0.5% penicillin-streptomycin (Sigma-Aldrich).

Techniques: Inhibition, Incubation, Migration, Imaging, Microscopy, Modification