du145 cell lines Search Results


hspc338  (CLS Cell Lines Service GmbH)
94
CLS Cell Lines Service GmbH hspc338
Hspc338, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/du145+cell+lines/DU-145+Cells/pm24848933__41435_2014_BFgene201420_MOESM11_ESM-592-0-43
Average 94 stars, based on 1 article reviews
hspc338 - by Bioz Stars, 2026-11
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lentiviral stable transduction du145 cells  (Genecopoeia)
94
Genecopoeia lentiviral stable transduction du145 cells
Lentiviral Stable Transduction Du145 Cells, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/du145+cell+lines/Human+cell+line+DU145+stably+expressing+CRISPR+Cas9%2C+randomly+inserted%2C+single+clone/pm32618200-36-3-25
Average 94 stars, based on 1 article reviews
lentiviral stable transduction du145 cells - by Bioz Stars, 2026-11
94/100 stars
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human prostate cancer cell line  (Genecopoeia)
94
Genecopoeia human prostate cancer cell line
Human Prostate Cancer Cell Line, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/du145+cell+lines/DU145+human+prostate+carcinoma+cancer+cell+line+dual-labeled+with+luciferase+and+GFP/10__3390_slash_app12157920-137-21-31
Average 94 stars, based on 1 article reviews
human prostate cancer cell line - by Bioz Stars, 2026-11
94/100 stars
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hela  (Korean Cell Line Bank)
90
Korean Cell Line Bank hela
EXP‐J reaction for lung cancer diagnosis. a) Heatmap showing the expression patterns of miR‐21 and miR‐92a in six cell lines (A549, WI‐38, <t>DU145,</t> HeLa, PC3, and SK‐BR‐3) using the EXP‐J assays. Each assay was repeated three times. Analytical agreement between EXP‐J assay and RT‐qPCR for b) miR‐21 and c) miR‐92a across cell lines. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. d) Schematic representation of EXP‐J reaction for lung cancer diagnosis (Created by Biorender.com). EXP‐J reaction was performed to detect miR‐21 and miR‐92a from the clinical plasma samples. Analysis of e,f) miR‐21 and g,h) miR‐92a from lung cancer patients (n = 20) and healthy controls (n = 20) by e,g) EXP‐J assay and f,h) RT‐qPCR. Both methods show the overexpression of miR‐21 and miR‐92a in plasma samples from lung cancer patients. Data points represent the mean of three replicates of each measurement for each method. Error bars indicate SD. Statistical analysis was performed using a two‐tailed t‐test with Welch correction where the asterisks (****p ≤ 0.0001) denote significant differences. All statistical analyses were performed at 95% confidence level. Correlation between EXP‐J assay and RT‐qPCR results for i) miR‐21 and j) miR‐92a detection. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. ROC curves for differentiation of lung cancer from healthy controls by k) miR‐21 and l) miR‐92a detection results using EXP‐J assay and RT‐qPCR. The AUC for each of the ROC curves is annotated.
Hela, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/du145+cell+lines/du145/pmc11600238-239-2-12
Average 90 stars, based on 1 article reviews
hela - by Bioz Stars, 2026-11
90/100 stars
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human prostate cancer cell line du145  (Kamei Co Ltd)
90
Kamei Co Ltd human prostate cancer cell line du145
EXP‐J reaction for lung cancer diagnosis. a) Heatmap showing the expression patterns of miR‐21 and miR‐92a in six cell lines (A549, WI‐38, <t>DU145,</t> HeLa, PC3, and SK‐BR‐3) using the EXP‐J assays. Each assay was repeated three times. Analytical agreement between EXP‐J assay and RT‐qPCR for b) miR‐21 and c) miR‐92a across cell lines. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. d) Schematic representation of EXP‐J reaction for lung cancer diagnosis (Created by Biorender.com). EXP‐J reaction was performed to detect miR‐21 and miR‐92a from the clinical plasma samples. Analysis of e,f) miR‐21 and g,h) miR‐92a from lung cancer patients (n = 20) and healthy controls (n = 20) by e,g) EXP‐J assay and f,h) RT‐qPCR. Both methods show the overexpression of miR‐21 and miR‐92a in plasma samples from lung cancer patients. Data points represent the mean of three replicates of each measurement for each method. Error bars indicate SD. Statistical analysis was performed using a two‐tailed t‐test with Welch correction where the asterisks (****p ≤ 0.0001) denote significant differences. All statistical analyses were performed at 95% confidence level. Correlation between EXP‐J assay and RT‐qPCR results for i) miR‐21 and j) miR‐92a detection. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. ROC curves for differentiation of lung cancer from healthy controls by k) miR‐21 and l) miR‐92a detection results using EXP‐J assay and RT‐qPCR. The AUC for each of the ROC curves is annotated.
Human Prostate Cancer Cell Line Du145, supplied by Kamei Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/du145+cell+lines/human+prostate+cancer+cell+line+du145/pm21986945-146-30-60
Average 90 stars, based on 1 article reviews
human prostate cancer cell line du145 - by Bioz Stars, 2026-11
90/100 stars
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du145 cell line  (Genetica Inc)
90
Genetica Inc du145 cell line
EXP‐J reaction for lung cancer diagnosis. a) Heatmap showing the expression patterns of miR‐21 and miR‐92a in six cell lines (A549, WI‐38, <t>DU145,</t> HeLa, PC3, and SK‐BR‐3) using the EXP‐J assays. Each assay was repeated three times. Analytical agreement between EXP‐J assay and RT‐qPCR for b) miR‐21 and c) miR‐92a across cell lines. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. d) Schematic representation of EXP‐J reaction for lung cancer diagnosis (Created by Biorender.com). EXP‐J reaction was performed to detect miR‐21 and miR‐92a from the clinical plasma samples. Analysis of e,f) miR‐21 and g,h) miR‐92a from lung cancer patients (n = 20) and healthy controls (n = 20) by e,g) EXP‐J assay and f,h) RT‐qPCR. Both methods show the overexpression of miR‐21 and miR‐92a in plasma samples from lung cancer patients. Data points represent the mean of three replicates of each measurement for each method. Error bars indicate SD. Statistical analysis was performed using a two‐tailed t‐test with Welch correction where the asterisks (****p ≤ 0.0001) denote significant differences. All statistical analyses were performed at 95% confidence level. Correlation between EXP‐J assay and RT‐qPCR results for i) miR‐21 and j) miR‐92a detection. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. ROC curves for differentiation of lung cancer from healthy controls by k) miR‐21 and l) miR‐92a detection results using EXP‐J assay and RT‐qPCR. The AUC for each of the ROC curves is annotated.
Du145 Cell Line, supplied by Genetica Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/du145+cell+lines/du145+cell+line/10__1074_slash_jbc__ra120__013694-166-0-6
Average 90 stars, based on 1 article reviews
du145 cell line - by Bioz Stars, 2026-11
90/100 stars
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prostate cancer cell line du145  (Janssen)
90
Janssen prostate cancer cell line du145
EXP‐J reaction for lung cancer diagnosis. a) Heatmap showing the expression patterns of miR‐21 and miR‐92a in six cell lines (A549, WI‐38, <t>DU145,</t> HeLa, PC3, and SK‐BR‐3) using the EXP‐J assays. Each assay was repeated three times. Analytical agreement between EXP‐J assay and RT‐qPCR for b) miR‐21 and c) miR‐92a across cell lines. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. d) Schematic representation of EXP‐J reaction for lung cancer diagnosis (Created by Biorender.com). EXP‐J reaction was performed to detect miR‐21 and miR‐92a from the clinical plasma samples. Analysis of e,f) miR‐21 and g,h) miR‐92a from lung cancer patients (n = 20) and healthy controls (n = 20) by e,g) EXP‐J assay and f,h) RT‐qPCR. Both methods show the overexpression of miR‐21 and miR‐92a in plasma samples from lung cancer patients. Data points represent the mean of three replicates of each measurement for each method. Error bars indicate SD. Statistical analysis was performed using a two‐tailed t‐test with Welch correction where the asterisks (****p ≤ 0.0001) denote significant differences. All statistical analyses were performed at 95% confidence level. Correlation between EXP‐J assay and RT‐qPCR results for i) miR‐21 and j) miR‐92a detection. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. ROC curves for differentiation of lung cancer from healthy controls by k) miR‐21 and l) miR‐92a detection results using EXP‐J assay and RT‐qPCR. The AUC for each of the ROC curves is annotated.
Prostate Cancer Cell Line Du145, supplied by Janssen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/du145+cell+lines/du145+cell+line/2R2829lM0ivDgITVEhiTw2bY2kSAhyCOR7bmqaoyPaCZzqWYe78yErrXsCVQakXc8YkjH9PXTXlA8fDS2-73-1-11
Average 90 stars, based on 1 article reviews
prostate cancer cell line du145 - by Bioz Stars, 2026-11
90/100 stars
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du145 human prostate cancer cell lines  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures du145 human prostate cancer cell lines
EXP‐J reaction for lung cancer diagnosis. a) Heatmap showing the expression patterns of miR‐21 and miR‐92a in six cell lines (A549, WI‐38, <t>DU145,</t> HeLa, PC3, and SK‐BR‐3) using the EXP‐J assays. Each assay was repeated three times. Analytical agreement between EXP‐J assay and RT‐qPCR for b) miR‐21 and c) miR‐92a across cell lines. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. d) Schematic representation of EXP‐J reaction for lung cancer diagnosis (Created by Biorender.com). EXP‐J reaction was performed to detect miR‐21 and miR‐92a from the clinical plasma samples. Analysis of e,f) miR‐21 and g,h) miR‐92a from lung cancer patients (n = 20) and healthy controls (n = 20) by e,g) EXP‐J assay and f,h) RT‐qPCR. Both methods show the overexpression of miR‐21 and miR‐92a in plasma samples from lung cancer patients. Data points represent the mean of three replicates of each measurement for each method. Error bars indicate SD. Statistical analysis was performed using a two‐tailed t‐test with Welch correction where the asterisks (****p ≤ 0.0001) denote significant differences. All statistical analyses were performed at 95% confidence level. Correlation between EXP‐J assay and RT‐qPCR results for i) miR‐21 and j) miR‐92a detection. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. ROC curves for differentiation of lung cancer from healthy controls by k) miR‐21 and l) miR‐92a detection results using EXP‐J assay and RT‐qPCR. The AUC for each of the ROC curves is annotated.
Du145 Human Prostate Cancer Cell Lines, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/du145+cell+lines/du145+human+prostate+cancer+cell+lines/pmc12071716-162-33-57
Average 90 stars, based on 1 article reviews
du145 human prostate cancer cell lines - by Bioz Stars, 2026-11
90/100 stars
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du 145 cell line  (Eisai Inc)
90
Eisai Inc du 145 cell line
EXP‐J reaction for lung cancer diagnosis. a) Heatmap showing the expression patterns of miR‐21 and miR‐92a in six cell lines (A549, WI‐38, <t>DU145,</t> HeLa, PC3, and SK‐BR‐3) using the EXP‐J assays. Each assay was repeated three times. Analytical agreement between EXP‐J assay and RT‐qPCR for b) miR‐21 and c) miR‐92a across cell lines. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. d) Schematic representation of EXP‐J reaction for lung cancer diagnosis (Created by Biorender.com). EXP‐J reaction was performed to detect miR‐21 and miR‐92a from the clinical plasma samples. Analysis of e,f) miR‐21 and g,h) miR‐92a from lung cancer patients (n = 20) and healthy controls (n = 20) by e,g) EXP‐J assay and f,h) RT‐qPCR. Both methods show the overexpression of miR‐21 and miR‐92a in plasma samples from lung cancer patients. Data points represent the mean of three replicates of each measurement for each method. Error bars indicate SD. Statistical analysis was performed using a two‐tailed t‐test with Welch correction where the asterisks (****p ≤ 0.0001) denote significant differences. All statistical analyses were performed at 95% confidence level. Correlation between EXP‐J assay and RT‐qPCR results for i) miR‐21 and j) miR‐92a detection. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. ROC curves for differentiation of lung cancer from healthy controls by k) miR‐21 and l) miR‐92a detection results using EXP‐J assay and RT‐qPCR. The AUC for each of the ROC curves is annotated.
Du 145 Cell Line, supplied by Eisai Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/du145+cell+lines/du+145+cell+line/10__1021_slash_np100430r-69-40-52
Average 90 stars, based on 1 article reviews
du 145 cell line - by Bioz Stars, 2026-11
90/100 stars
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prostein-negative prostate tumor cell line du-145  (Verlag GmbH)
90
Verlag GmbH prostein-negative prostate tumor cell line du-145
EXP‐J reaction for lung cancer diagnosis. a) Heatmap showing the expression patterns of miR‐21 and miR‐92a in six cell lines (A549, WI‐38, <t>DU145,</t> HeLa, PC3, and SK‐BR‐3) using the EXP‐J assays. Each assay was repeated three times. Analytical agreement between EXP‐J assay and RT‐qPCR for b) miR‐21 and c) miR‐92a across cell lines. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. d) Schematic representation of EXP‐J reaction for lung cancer diagnosis (Created by Biorender.com). EXP‐J reaction was performed to detect miR‐21 and miR‐92a from the clinical plasma samples. Analysis of e,f) miR‐21 and g,h) miR‐92a from lung cancer patients (n = 20) and healthy controls (n = 20) by e,g) EXP‐J assay and f,h) RT‐qPCR. Both methods show the overexpression of miR‐21 and miR‐92a in plasma samples from lung cancer patients. Data points represent the mean of three replicates of each measurement for each method. Error bars indicate SD. Statistical analysis was performed using a two‐tailed t‐test with Welch correction where the asterisks (****p ≤ 0.0001) denote significant differences. All statistical analyses were performed at 95% confidence level. Correlation between EXP‐J assay and RT‐qPCR results for i) miR‐21 and j) miR‐92a detection. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. ROC curves for differentiation of lung cancer from healthy controls by k) miR‐21 and l) miR‐92a detection results using EXP‐J assay and RT‐qPCR. The AUC for each of the ROC curves is annotated.
Prostein Negative Prostate Tumor Cell Line Du 145, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/du145+cell+lines/prostein+negative+prostate+tumor+cell+line+du+145/pm15048720-116-16-9
Average 90 stars, based on 1 article reviews
prostein-negative prostate tumor cell line du-145 - by Bioz Stars, 2026-11
90/100 stars
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du-145 cell line  (Inserm Transfert)
90
Inserm Transfert du-145 cell line
EXP‐J reaction for lung cancer diagnosis. a) Heatmap showing the expression patterns of miR‐21 and miR‐92a in six cell lines (A549, WI‐38, <t>DU145,</t> HeLa, PC3, and SK‐BR‐3) using the EXP‐J assays. Each assay was repeated three times. Analytical agreement between EXP‐J assay and RT‐qPCR for b) miR‐21 and c) miR‐92a across cell lines. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. d) Schematic representation of EXP‐J reaction for lung cancer diagnosis (Created by Biorender.com). EXP‐J reaction was performed to detect miR‐21 and miR‐92a from the clinical plasma samples. Analysis of e,f) miR‐21 and g,h) miR‐92a from lung cancer patients (n = 20) and healthy controls (n = 20) by e,g) EXP‐J assay and f,h) RT‐qPCR. Both methods show the overexpression of miR‐21 and miR‐92a in plasma samples from lung cancer patients. Data points represent the mean of three replicates of each measurement for each method. Error bars indicate SD. Statistical analysis was performed using a two‐tailed t‐test with Welch correction where the asterisks (****p ≤ 0.0001) denote significant differences. All statistical analyses were performed at 95% confidence level. Correlation between EXP‐J assay and RT‐qPCR results for i) miR‐21 and j) miR‐92a detection. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. ROC curves for differentiation of lung cancer from healthy controls by k) miR‐21 and l) miR‐92a detection results using EXP‐J assay and RT‐qPCR. The AUC for each of the ROC curves is annotated.
Du 145 Cell Line, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/du145+cell+lines/du+145+cell+line/pm24768038-55-21-29
Average 90 stars, based on 1 article reviews
du-145 cell line - by Bioz Stars, 2026-11
90/100 stars
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du-145  (Dainippon Sumitomo)
90
Dainippon Sumitomo du-145
EXP‐J reaction for lung cancer diagnosis. a) Heatmap showing the expression patterns of miR‐21 and miR‐92a in six cell lines (A549, WI‐38, <t>DU145,</t> HeLa, PC3, and SK‐BR‐3) using the EXP‐J assays. Each assay was repeated three times. Analytical agreement between EXP‐J assay and RT‐qPCR for b) miR‐21 and c) miR‐92a across cell lines. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. d) Schematic representation of EXP‐J reaction for lung cancer diagnosis (Created by Biorender.com). EXP‐J reaction was performed to detect miR‐21 and miR‐92a from the clinical plasma samples. Analysis of e,f) miR‐21 and g,h) miR‐92a from lung cancer patients (n = 20) and healthy controls (n = 20) by e,g) EXP‐J assay and f,h) RT‐qPCR. Both methods show the overexpression of miR‐21 and miR‐92a in plasma samples from lung cancer patients. Data points represent the mean of three replicates of each measurement for each method. Error bars indicate SD. Statistical analysis was performed using a two‐tailed t‐test with Welch correction where the asterisks (****p ≤ 0.0001) denote significant differences. All statistical analyses were performed at 95% confidence level. Correlation between EXP‐J assay and RT‐qPCR results for i) miR‐21 and j) miR‐92a detection. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. ROC curves for differentiation of lung cancer from healthy controls by k) miR‐21 and l) miR‐92a detection results using EXP‐J assay and RT‐qPCR. The AUC for each of the ROC curves is annotated.
Du 145, supplied by Dainippon Sumitomo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/du145+cell+lines/human+prostate+cancer+cell+lines+du145/10__2974_slash_kmj__58__363-34-9-13
Average 90 stars, based on 1 article reviews
du-145 - by Bioz Stars, 2026-11
90/100 stars
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Image Search Results


EXP‐J reaction for lung cancer diagnosis. a) Heatmap showing the expression patterns of miR‐21 and miR‐92a in six cell lines (A549, WI‐38, DU145, HeLa, PC3, and SK‐BR‐3) using the EXP‐J assays. Each assay was repeated three times. Analytical agreement between EXP‐J assay and RT‐qPCR for b) miR‐21 and c) miR‐92a across cell lines. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. d) Schematic representation of EXP‐J reaction for lung cancer diagnosis (Created by Biorender.com). EXP‐J reaction was performed to detect miR‐21 and miR‐92a from the clinical plasma samples. Analysis of e,f) miR‐21 and g,h) miR‐92a from lung cancer patients (n = 20) and healthy controls (n = 20) by e,g) EXP‐J assay and f,h) RT‐qPCR. Both methods show the overexpression of miR‐21 and miR‐92a in plasma samples from lung cancer patients. Data points represent the mean of three replicates of each measurement for each method. Error bars indicate SD. Statistical analysis was performed using a two‐tailed t‐test with Welch correction where the asterisks (****p ≤ 0.0001) denote significant differences. All statistical analyses were performed at 95% confidence level. Correlation between EXP‐J assay and RT‐qPCR results for i) miR‐21 and j) miR‐92a detection. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. ROC curves for differentiation of lung cancer from healthy controls by k) miR‐21 and l) miR‐92a detection results using EXP‐J assay and RT‐qPCR. The AUC for each of the ROC curves is annotated.

Journal: Advanced Science

Article Title: Unveiling Cas12j Trans‐Cleavage Activity for CRISPR Diagnostics: Application to miRNA Detection in Lung Cancer Diagnosis

doi: 10.1002/advs.202402580

Figure Lengend Snippet: EXP‐J reaction for lung cancer diagnosis. a) Heatmap showing the expression patterns of miR‐21 and miR‐92a in six cell lines (A549, WI‐38, DU145, HeLa, PC3, and SK‐BR‐3) using the EXP‐J assays. Each assay was repeated three times. Analytical agreement between EXP‐J assay and RT‐qPCR for b) miR‐21 and c) miR‐92a across cell lines. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. d) Schematic representation of EXP‐J reaction for lung cancer diagnosis (Created by Biorender.com). EXP‐J reaction was performed to detect miR‐21 and miR‐92a from the clinical plasma samples. Analysis of e,f) miR‐21 and g,h) miR‐92a from lung cancer patients (n = 20) and healthy controls (n = 20) by e,g) EXP‐J assay and f,h) RT‐qPCR. Both methods show the overexpression of miR‐21 and miR‐92a in plasma samples from lung cancer patients. Data points represent the mean of three replicates of each measurement for each method. Error bars indicate SD. Statistical analysis was performed using a two‐tailed t‐test with Welch correction where the asterisks (****p ≤ 0.0001) denote significant differences. All statistical analyses were performed at 95% confidence level. Correlation between EXP‐J assay and RT‐qPCR results for i) miR‐21 and j) miR‐92a detection. Data points represent the mean of three replicates of each measurement for each method. Linear fitting of data points was performed to generate linear correlation curves. Dashed lines represent 95% confidence bands of the best fit line. ROC curves for differentiation of lung cancer from healthy controls by k) miR‐21 and l) miR‐92a detection results using EXP‐J assay and RT‐qPCR. The AUC for each of the ROC curves is annotated.

Article Snippet: A549, WI‐38, DU145, HeLa, PC3, and SK‐BR‐3 cell lines were obtained from Korean Cell Line Bank (Seoul, Korea).

Techniques: Expressing, Quantitative RT-PCR, Over Expression, Two Tailed Test