Journal: Brain communications

Article Title: Spatial selectivity of ATase inhibition in mouse models of Charcot-Marie-Tooth disease.

doi: 10.1093/braincomms/fcae232

Figure Lengend Snippet: Figure 4 Phenotypic assessment of C3-PMP22 mice. (A) Phenotypic severity score represented as a sum of ledge, gait and hindlimb clasping at different ages (n = 7/group). **P < 0.005 and

Article Snippet: Cells were transiently transfected with Lipofectamine 2000 (Invitrogen #11668019) using the following constructs: mCherry-ER-3 plasmid, a gift from Michael Davidson (Addgene plasmid #55041); PMP22 human-tagged ORF clone (OriGene #RC216500); and MPZ human-tagged ORF clone (OriGene #RC202450).

Techniques:

Journal: PLoS ONE

Article Title: Inhibition of β-catenin dependent WNT signalling upregulates the transcriptional repressor NR0B1 and downregulates markers of an A9 phenotype in human embryonic stem cell-derived dopaminergic neurons: Implications for Parkinson’s disease

doi: 10.1371/journal.pone.0261730

Figure Lengend Snippet: Panels (a) and (b) respectively show the effects of the WNT modulators and NEUROD1 overexpression upon NR0B1 . Panels (c) and (d) show effects upon NR0B2 expression. Results are expressed as mean ± SEM of three (biological) replicate experiments. Analysis of panel (a) was via one-way ANOVA with post-hoc Dunnett’s test. For panels (b) and (d) analysis was via Student’s t-tests (at each time point as in ). For panel (c) analysis was via a non-parametric Kruskal-Wallis test with post-hoc Dunn’s test to accommodate undetectable levels of NR0B2 in two out of three samples, both undetectable values were assigned to 0.0028 (equal to the lowest value detected).

Article Snippet: At this time, cultures were transfected with NEUROD1 (SC118625, Origene), NR0B1 (SC319624, Origene), NR0B2 (SC122920, Origene) or blank plasmids (PCMV6XL5, Origene) according to manufacturer’s instructions.

Techniques: Over Expression, Expressing

Journal: PLoS ONE

Article Title: Inhibition of β-catenin dependent WNT signalling upregulates the transcriptional repressor NR0B1 and downregulates markers of an A9 phenotype in human embryonic stem cell-derived dopaminergic neurons: Implications for Parkinson’s disease

doi: 10.1371/journal.pone.0261730

Figure Lengend Snippet: Expression patterns following transfection with NR0B1 (green), NR0B2 (red) or blank (blue) plasmids show that NR0B1 transfection increases both NR0B1 and NR0B2 and impacts expression of NEUROD1 , TH , KCNJ6 , LMX1A and TUBB3 . Control wells underwent transfection with an empty plasmid. Results are expressed as mean ± SEM of three (biological) replicate experiments. Analysis was performed with One-way ANOVA followed by post-hoc Dunnett’s test where each post transfection timepoint represented a repeated measure (t = 0 was not included in analyses). * and ** indicate P<0.05 and P<0.01, respectively. shows increased NR0B1 and NR0B2 immunolabelling up to six days after transfection.

Article Snippet: At this time, cultures were transfected with NEUROD1 (SC118625, Origene), NR0B1 (SC319624, Origene), NR0B2 (SC122920, Origene) or blank plasmids (PCMV6XL5, Origene) according to manufacturer’s instructions.

Techniques: Expressing, Transfection, Control, Plasmid Preparation