Journal: Antonie Van Leeuwenhoek

Article Title: New genus-specific primers for PCR identification of Rubrobacter strains

doi: 10.1007/s10482-019-01314-3

Figure Lengend Snippet: Type strains of Rubrobacter species and those of related genera, their growth requirements and 16S rRNA gene sequence accession numbers

Article Snippet: Rubrobacter aplysinae , DSM 27440 T , DSMZ medium No. 514 + 1% starch , 25 , GU318365 , Kämpfer et al. ( ) .

Techniques: Sequencing, Starch

Journal: Antonie Van Leeuwenhoek

Article Title: New genus-specific primers for PCR identification of Rubrobacter strains

doi: 10.1007/s10482-019-01314-3

Figure Lengend Snippet: Conserved nucleotide regions of 16S rRNA genes of Rubrobacter type strains used to design the specific primers Rubro223f and Rubro454r. The bar represents the 16S rRNA gene sequence of Escherichia coli ; black boxes indicate conserved regions and the grey ones variable regions (V) with corresponding numbers (Brosius et al. ; Yarza et al. ). Arrows above the bar represent the position of the primers within the 16S rRNA gene sequence. Nucleotide alignment for primers Rubro223f and Rubro454r and for primer Rubro749r (Holmes et al. ) are highlighted in orange boxes and nucleotides in white represent those unique to the genus Rubrobacter and hence absent in the type strains of species classified in the genera Conexibacter , Gaiella , Parviterribacter , Patulibacter, Solirubrobacter and Thermoleophilum . (Color figure online)

Article Snippet: Rubrobacter aplysinae , DSM 27440 T , DSMZ medium No. 514 + 1% starch , 25 , GU318365 , Kämpfer et al. ( ) .

Techniques: Sequencing

Journal: Antonie Van Leeuwenhoek

Article Title: New genus-specific primers for PCR identification of Rubrobacter strains

doi: 10.1007/s10482-019-01314-3

Figure Lengend Snippet: Assessment of the specificity of primers Rubro223f and Rubro454r using the RDP database

Article Snippet: Rubrobacter aplysinae , DSM 27440 T , DSMZ medium No. 514 + 1% starch , 25 , GU318365 , Kämpfer et al. ( ) .

Techniques:

Journal: Antonie Van Leeuwenhoek

Article Title: New genus-specific primers for PCR identification of Rubrobacter strains

doi: 10.1007/s10482-019-01314-3

Figure Lengend Snippet: Verification of the specificity of primers Rubro223f and Rubro454r in PCR runs using genomic DNA extracted from Rubrobacter type strains and corresponding strains of the closely-related genera. Electrophoresis in 2% agarose gels shows PCR amplification of a region of 267 nt that was only found in the Rubrobacter strains

Article Snippet: Rubrobacter aplysinae , DSM 27440 T , DSMZ medium No. 514 + 1% starch , 25 , GU318365 , Kämpfer et al. ( ) .

Techniques: Electrophoresis, Amplification