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Image Search Results
Journal: Molecular Cell
Article Title: BUB1 and CENP-U, Primed by CDK1, Are the Main PLK1 Kinetochore Receptors in Mitosis
doi: 10.1016/j.molcel.2020.10.040
Figure Lengend Snippet:
Article Snippet: After blocking the cells with either 3% BSAor 5% boiled goat serum in PHEM buffer supplemented with 0.1% Triton X-100 (PHEM-T), they were incubated at room temperature for 1-2 or 16 hours at 4°C with the following primary antibodies: anti-CENP-QU (in-house generated; goat 1:100), anti CENP-O (rabbit; 1:500) , anti-PLK1 (Abcam #ab17057, mouse monoclonal antibody; 1:300), anti-H3-pS10 (Abcam # ab5176, rabbit polyclonal antibody, 1:2000), rabbit anti-CEP135 (gift from Alex Bird [ ], 1:3000), anti-BUB1 (Abcam #ab9000, rabbit polyclonal antibody, 1:1000), anti-BubR1 (rabbit polyclonal, Abcam, ab70544, 1:500), anti-Aurora B (mouse monoclonal, Abcam, ab3609, 1:500),
Techniques: Affinity Purification, Virus, Recombinant, Transfection, Labeling, Protease Inhibitor, Chromatography, Staining, Software