Journal: Aging Cell

Article Title: Aging‐associated decrease of PGC ‐1α promotes pain chronification

doi: 10.1111/acel.14177

Figure Lengend Snippet: Inhibition of interneuron activity in S1HL cortex results in hyperalgesia in older mice. (a) Representative immunostaining images. Brain slices throughout S1HL cortex were selected and stained with anti‐GABA receptor. Immunofluorescence imaging showed 80% dTomato+ cells were GABA+ cells in S1HL cortex. N = 4, data are represented as mean ± SD. (b) Diagram and sketch depict experiment design. AAV9‐Dlx‐Gi DREADD‐dTomato was injected into S1HL of adult mice. A representative image of brain slice with box region zoomed verified viral transfection in S1HL cortex. (c) Mechanical withdrawal threshold was significantly decreased after C21 administrated. (d) Hindpaw withdrawal latency in response to S1HL inhibition. Comparing with the mice subject to vector virus, mice injected Gi GREADDs exhibited significant decreasing in hindpaw withdrawal latency after C21 administration. N = 8, data are presented as mean ± SD, two‐tailed paired t test, ** p < 0.01, NS: not significant ( p > 0.05).

Article Snippet: C21 (Tocris, catalog 5548) was intraperitoneally administered at 1 mg/kg.

Techniques: Inhibition, Activity Assay, Immunostaining, Staining, Immunofluorescence, Imaging, Injection, Slice Preparation, Transfection, Plasmid Preparation, Virus, Two Tailed Test

Journal: Aging Cell

Article Title: Aging‐associated decrease of PGC ‐1α promotes pain chronification

doi: 10.1111/acel.14177

Figure Lengend Snippet: Activation of interneurons in S1HL alleviates aging‐associated pain chronification. (a) Diagram and sketch depict experimental design. AAV9‐Dlx‐Gq DREADD‐dTomato was injected into S1HL of adult mice. Following baseline behavioral test, mice received CCI surgery and C21 was administrated at day 14 after surgery. Pain behaviors were assessed 30 min after C21. (b) Mechanical withdrawal threshold. N = 8 mice, in mice received vector virus, before versus after surgery *** p < 0.001, before versus after C21 *** p < 0.001; in mice received Gq DREADD, before versus after surgery *** p < 0.001, before versus after C21 ** p < 0.01. (c) Hindpaw withdrawal latency. Comparing with mice subjected to vector virus, mice received Gq DREADD injection in S1HL and C21 administration exhibited significant increasing in mechanical withdrawal threshold and hindpaw withdrawal latency. N = 8, data are presented as mean ± SD in panel B. One‐way ANOVA followed by Tukey post hoc test was performed for the comparison. ** p < 0.01, *** p < 0.001. NS: not significant ( p > 0.05). (d) Representative immunofluorescent images. Brain slices throughout S1HL cortex were selected and stained with anti‐GABA. Immunofluorescence imaging showed 80% dTomato+ cells were GABA + cells in S1HL cortex. N = 4, data are represented as mean ± SD.

Article Snippet: C21 (Tocris, catalog 5548) was intraperitoneally administered at 1 mg/kg.

Techniques: Activation Assay, Injection, Plasmid Preparation, Virus, Comparison, Staining, Immunofluorescence, Imaging

Journal: bioRxiv

Article Title: Selective reduction of KCNA4 in vulnerable glutamatergic-serotonin neurons of the dorsal raphe nucleus in Alzheimer’s Disease

doi: 10.1101/2025.10.17.683113

Figure Lengend Snippet: A: Graphical overview of the htau DREADDs experiment. Htau mice were intracranially administered hM3Dq (n = 14), hM4Di (n=14), or control virus (n = 9) at 2 months’ age. DREADDs agonist (CNO DHC) was administered via water bottle for 6 weeks. B , C : RNAscope assessment of Fos expression in virally transduced Tph2 + cells before CNO washout (acute) and after 7-day washout. Two data points were collected per mouse in ( C ). No statistical test was performed in ( C ), data were qualitatively compared. D,E : Novel objection recognition (NOR). D : Percent preference for novel object and E , discrimination index. 1w ANOVA with Tukey’s post hoc . F,G : Novelty-induced suppression of feeding (NSF). F : latency to feed and G , time spent in the corners of the arena during 10-minute assessment period. H–K : Elevated plus maze (EPM) task. H : Percent of total time spent in open arm, I , entries to open arm, J , total distance traveled, and K , average velocity during the task. L–R : Three-chamber social interaction test (SIT). L : Total social interaction time, M , number of social interactions, N , social interaction time as a percentage of time spent in the social chamber, and O , time spent in the center chamber. P–R Representative heat maps, normalized within trial. D–F,H–K : 1w ANOVA w/ Dunnett’s post hoc . G , 1w Welch’s ANOVA w/Dunnett’s post hoc . L-O : 1w ANOVA w/ Tukey’s post hoc .* p < 0.05, ** p < 0.01

Article Snippet: After a 7-day recovery period, mice were continuously administered the DREADD agonist CNO DHC (clozapine-n-oxide dihydrochloride, Hello Bio #HB6149, Princeton, NJ, USA) at a concentration of 6.06 mg/ml (equivalent to 5 mg/ml CNO) via water bottle in a home-cage environment.

Techniques: Control, Virus, RNAscope, Expressing