Journal: Journal of Functional Biomaterials

Article Title: Influence of Micro-Nanostructured Anatase-Coated SLA Titanium on Macrophage Behavior

doi: 10.3390/jfb17030111

Figure Lengend Snippet: Behavior of M0-, M1-, and M2-MDMs on differently modified titanium Surfaces. ( A ) Representative CLSM images showing M0-, M1-, and M2-MDMs on coverslip, Ti-machined, Ti-SLA, and Ti-SLA-anatase surfaces after 48 h of incubation. The cytoskeleton (F-actin) was stained with FITC-Phalloidin (green), and nuclei were stained with DRAQ5 (purple). ( B ) Representative SEM images of M0-, M1, and M2 macrophages on coverslip and titanium surface modifications. ( C ) Metabolic activity was assessed using the CCK-8 assay, with values normalized to coverslip M0 (set as 100%). Data are presented as mean ± SEM. n = 3. No significant differences were observed between groups (Friedman test with Dunn’s multiple comparisons test).

Article Snippet: Samples were blocked in 1% bovine serum albumin (BSA; VWR International, Leuven, Belgium; Cat No. 422361V) and 0.05% Tween-20 (Roth, Karlsruhe, Germany; Cat. No. 9127.1) in PBS for 30 min. Cytoskeletal staining was performed using 5 μg/mL phalloidin–FITC (Sigma Aldrich, Saint Louis, MI, USA; Cat. No. P5282) in blocking buffer for 1 h, followed by nuclear staining with 10 μM DRAQ5 (Miltenyi Biotec, Bergisch Gladbach, Germany; Cat No. 130-117-344) for 15 min at RT.

Techniques: Modification, Incubation, Staining, Activity Assay, CCK-8 Assay

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of left ventricular tissue from a 2-day-old newborn. The ConA expression (green channel, A1 ) shows affinity for the cellular membrane of the LV. Nuclei are stained with DRAQ5 (red channel, A2 ). Merged images are shown for all samples ( A3 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Expressing, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of left ventricular tissue from cats in control group. ( A ) Comparison of BS expression (green channel, A1 ) with WGA expression (blue channel, A2 ) in a 6-month-old kitten. The BS expression is nearly absent, while WGA shows preferential binding to the cellular membrane. ( B ) Comparison of Tomato expression (green channel, B1 ) with ConA expression (blue channel, B2 ) in a 7-year-old mature adult. The Tomato shows strong membrane localization, whereas ConA displays cytoplasmic affinity. Sporadic lipofuscin (asterisk *) accumulation is observed in the cytoplasm and is visible across all channels ( B1 – B4 ). ( C ) Comparison of Tomato expression (green channel, C1 ) with ConA expression (blue channel, C2 ) in a 14-year-old senior cat. The Tomato shows strong affinity for both the cellular membrane and vascular wall, while ConA displays more pronounced cytoplasmic localization ( B2 ). Marked lipofuscin (asterisk *) accumulation is present in the cytoplasm, visible across all channels ( C1 – C4 ). Nuclei are stained with DRAQ5 (red channel; A3 , B3 , C3 ). Merged images are shown for all samples ( A4 , B4 , C4 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Control, Comparison, Expressing, Binding Assay, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of right ventricular tissue from a 2-day-old newborn. The ConA expression (green channel; A1 ) shows affinity for the cellular membrane of the RV. Nuclei are stained with DRAQ5 (red channel; A2 ). Merged images are shown for all samples ( A3 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Expressing, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of the right ventricular tissue from cats. ( A ) Comparison of Tomato expression (green channel, A1 ) with ConA expression (blue channel, A2 ) in a 6-month-old kitten. Both lectins exhibit strong membrane localization. ( B ) Comparison of BS expression (green channel, B1 ) with WGA expression (blue channel, B2 ) in a 3-year-old young adult with HCM, where both lectins show moderate cytoplasmic affinity. Nuclei are stained with DRAQ5 (red channel; A3 , B3 ). Merged images are shown for all samples ( A4 , B4 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Comparison, Expressing, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of atrial tissue from a 2-day-old newborn. The ConA expression (green channel; A1 ) shows affinity for both the cellular membrane and cytoplasm of the AT. Nuclei are stained with DRAQ5 (red channel; A2 ). Merged images are shown for all samples ( A3 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Expressing, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of atrial tissue from cats. ( A ) Comparison of Tomato (green channel, A1 ) and ConA (blue channel, A2 ) expression in a 4-year-old young adult with HCM. Both lectins bind to the cellular membrane, with Tomato exhibiting more intense binding. ( B ) Comparison of Tomato (green channel, B1 ) and ConA (blue channel, B2 ) expression in a 5-year-old young adult with HCM. Both lectins show affinity for both the cellular membrane and cytoplasm, with stronger membrane localization. ( C ) Comparison of BS expression (green channel, C1 ) and WGA expression (blue channel, C2 ) in a 12.5-year-old senior cat. The BS shows low membrane affinity and higher binding to red blood cells (asterisk *) ( C1 , C4 ), while WGA demonstrates strong membrane affinity and moderate red blood cells (asterisk *) and cytoplasmic localization ( C2 , C4 ). Sporadic lipofuscin accumulation is present in the cytoplasm, visible across WGA and Draq5 channels as high intensity points ( C2 , C3 ) and can be observed as magenta points ( C4 ). Nuclei are stained with DRAQ5 (red channel; A3 , B3 , C3 ). Merged images are shown for all samples ( A4 , B4 , C4 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Comparison, Expressing, Membrane, Binding Assay, Staining

Journal: iScience

Article Title: Adenosine deaminase and deoxyadenosine regulate intracellular immune response in C. elegans

doi: 10.1016/j.isci.2025.111950

Figure Lengend Snippet:

Article Snippet: DRAQ5 , Novus Biologicals , NBP2-81125.

Techniques: Purification, Virus, Recombinant, Cell Culture, Control, Plasmid Preparation, Software, Microscopy