Journal: bioRxiv

Article Title: Starve-Feed Cycles Direct Quiescence to Proliferation Transitions in Drosophila Follicle Stem Cells via Transcriptional Regulation

doi: 10.64898/2026.01.22.701111

Figure Lengend Snippet: A-F) Germaria isolated from transgenic fly lines with Gal4 inserted into genes known to be expressed and functional in the germarium. Probes complementary to Gal4 (green) and Fas3 (red) were used for RNA in situ hybridization using the RNAScope approach. Nuclei are labeled (Draq5, blue). Scale bars are indicated for each image.

Article Snippet: Draq5 was used to label nuclei (1:1000, Miltenyi Biotec Cat# 130-117-344).

Techniques: Isolation, Transgenic Assay, Functional Assay, RNA In Situ Hybridization, RNAscope, Labeling

Journal: bioRxiv

Article Title: Starve-Feed Cycles Direct Quiescence to Proliferation Transitions in Drosophila Follicle Stem Cells via Transcriptional Regulation

doi: 10.64898/2026.01.22.701111

Figure Lengend Snippet: RNAScope of transgenic lines bearing insertion of Gal4 in Q->P regulatory candidates, using probes against Gal4 (green) and Fas3 (red). Nuclei are labeled (Draq5, blue). Candidates with no detectable Gal4 (top) or broad, non-specific expression (middle, bottom) are shown. Scale bars are indicated for each image.

Article Snippet: Draq5 was used to label nuclei (1:1000, Miltenyi Biotec Cat# 130-117-344).

Techniques: RNAscope, Transgenic Assay, Labeling, Expressing

Journal: bioRxiv

Article Title: Starve-Feed Cycles Direct Quiescence to Proliferation Transitions in Drosophila Follicle Stem Cells via Transcriptional Regulation

doi: 10.64898/2026.01.22.701111

Figure Lengend Snippet: RNAScope of transgenic lines bearing insertion of Gal4 in Q->P regulatory candidates, using probes against Gal4 (green) and Fas3 (red). Nuclei are labeled (Draq5, blue). Candidates with Gal4 in cells other than FSCs (A), or enriched in FSCs (B) are shown. The FSC region is indicated by a dotted oval in B. Scale bars are indicated for each image.

Article Snippet: Draq5 was used to label nuclei (1:1000, Miltenyi Biotec Cat# 130-117-344).

Techniques: RNAscope, Transgenic Assay, Labeling

Journal: bioRxiv

Article Title: Starve-Feed Cycles Direct Quiescence to Proliferation Transitions in Drosophila Follicle Stem Cells via Transcriptional Regulation

doi: 10.64898/2026.01.22.701111

Figure Lengend Snippet: A-E) RNAScope of germaria bearing Gal4 expression in the indicated genes. Probes against Gal4 (green) and Fas3 (red) indicate gene expression in nutrient-restricted (“starved”) or 6-hours fed germaria. Nuclei are labeled (Draq5, blue). The FSC niche is outlined by a dashed oval. Scale bars are indicated for each image. A) Feeding-dependence of previously characterized genes. B) Candidates in the Aq2 cluster, which are enriched in the TU-tagged fraction and increase by 3 hours after feeding. C) Candidates in the Aq1 cluster, which are enriched in the TU-tagged fraction and increase in both the Input and the streptavidin-precipitated fraction by 3 hours after feeding. D) Candidates in the bq1 cluster, which are enriched in the TU-tagged fraction and increase in both the Input and the streptavidin-precipitated fraction by 6 hours after feeding. E) nrv1-Gal4 germarium, a non-feeding-dependent example that shares function with nrv2 , a feeding-dependent gene.

Article Snippet: Draq5 was used to label nuclei (1:1000, Miltenyi Biotec Cat# 130-117-344).

Techniques: RNAscope, Expressing, Gene Expression, Labeling

Journal: Memórias do Instituto Oswaldo Cruz

Article Title: Past and future of trypanosomatids high-throughput phenotypic screening

doi: 10.1590/0074-02760210402

Figure Lengend Snippet: HCS-based assays employed in trypanosomatid drug discovery

Article Snippet: , L. donovani , Laboratory strain: MHOM/SD/62/1S-CL2D , Intracellular amastigotes , None , Human acute monocytic leukemia cells (THP-1) , Number of host cells (cytotoxicity evaluation), number of amastigotes per cell and infection ratio , Parasites DNA spots as well as cell nucleus and cytoplasm detected by Draq5 , 1,742 bioactive compounds from MedChem Express , Primary screening and cytotoxicity evaluation performed in the same assay. A similar method was used to generate the dose-response curves. Protocol based on a previous report (82) , (95).

Techniques: Infection, Immunostaining, Cytotoxicity Assay, Activity Assay, Luciferase, Proliferation Assay, Imaging, Time-Kill Assay, Derivative Assay, Expressing, Drug discovery, Staining, Labeling, SYBR Green Assay

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of left ventricular tissue from a 2-day-old newborn. The ConA expression (green channel, A1 ) shows affinity for the cellular membrane of the LV. Nuclei are stained with DRAQ5 (red channel, A2 ). Merged images are shown for all samples ( A3 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Expressing, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of left ventricular tissue from cats in control group. ( A ) Comparison of BS expression (green channel, A1 ) with WGA expression (blue channel, A2 ) in a 6-month-old kitten. The BS expression is nearly absent, while WGA shows preferential binding to the cellular membrane. ( B ) Comparison of Tomato expression (green channel, B1 ) with ConA expression (blue channel, B2 ) in a 7-year-old mature adult. The Tomato shows strong membrane localization, whereas ConA displays cytoplasmic affinity. Sporadic lipofuscin (asterisk *) accumulation is observed in the cytoplasm and is visible across all channels ( B1 – B4 ). ( C ) Comparison of Tomato expression (green channel, C1 ) with ConA expression (blue channel, C2 ) in a 14-year-old senior cat. The Tomato shows strong affinity for both the cellular membrane and vascular wall, while ConA displays more pronounced cytoplasmic localization ( B2 ). Marked lipofuscin (asterisk *) accumulation is present in the cytoplasm, visible across all channels ( C1 – C4 ). Nuclei are stained with DRAQ5 (red channel; A3 , B3 , C3 ). Merged images are shown for all samples ( A4 , B4 , C4 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Control, Comparison, Expressing, Binding Assay, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of right ventricular tissue from a 2-day-old newborn. The ConA expression (green channel; A1 ) shows affinity for the cellular membrane of the RV. Nuclei are stained with DRAQ5 (red channel; A2 ). Merged images are shown for all samples ( A3 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Expressing, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of the right ventricular tissue from cats. ( A ) Comparison of Tomato expression (green channel, A1 ) with ConA expression (blue channel, A2 ) in a 6-month-old kitten. Both lectins exhibit strong membrane localization. ( B ) Comparison of BS expression (green channel, B1 ) with WGA expression (blue channel, B2 ) in a 3-year-old young adult with HCM, where both lectins show moderate cytoplasmic affinity. Nuclei are stained with DRAQ5 (red channel; A3 , B3 ). Merged images are shown for all samples ( A4 , B4 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Comparison, Expressing, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of atrial tissue from a 2-day-old newborn. The ConA expression (green channel; A1 ) shows affinity for both the cellular membrane and cytoplasm of the AT. Nuclei are stained with DRAQ5 (red channel; A2 ). Merged images are shown for all samples ( A3 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Expressing, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of atrial tissue from cats. ( A ) Comparison of Tomato (green channel, A1 ) and ConA (blue channel, A2 ) expression in a 4-year-old young adult with HCM. Both lectins bind to the cellular membrane, with Tomato exhibiting more intense binding. ( B ) Comparison of Tomato (green channel, B1 ) and ConA (blue channel, B2 ) expression in a 5-year-old young adult with HCM. Both lectins show affinity for both the cellular membrane and cytoplasm, with stronger membrane localization. ( C ) Comparison of BS expression (green channel, C1 ) and WGA expression (blue channel, C2 ) in a 12.5-year-old senior cat. The BS shows low membrane affinity and higher binding to red blood cells (asterisk *) ( C1 , C4 ), while WGA demonstrates strong membrane affinity and moderate red blood cells (asterisk *) and cytoplasmic localization ( C2 , C4 ). Sporadic lipofuscin accumulation is present in the cytoplasm, visible across WGA and Draq5 channels as high intensity points ( C2 , C3 ) and can be observed as magenta points ( C4 ). Nuclei are stained with DRAQ5 (red channel; A3 , B3 , C3 ). Merged images are shown for all samples ( A4 , B4 , C4 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Comparison, Expressing, Membrane, Binding Assay, Staining

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Accurate Titration of Infectious AAV Particles Requires Measurement of Biologically Active Vector Genomes and Suitable Controls

doi: 10.1016/j.omtm.2018.07.004

Figure Lengend Snippet: Immunofluorescence Analysis of Intracellular Localization of AAV8 Particles (A) Representative pictures of infected HeLa cells. Cells were non-infected (no AAV) or infected with AAV8 control or AAV8ΔVP1 at a multiplicity of 20,000 VG/cell, and then they were fixed after 1, 5, or 16 hr. Cell nuclei stained with DraQ5 appear in red, and assembled AAV8 particles stained with Alexa Fluor 555 appear in blue, green, or cyan, depending on their localization (cytoplasmic, intranuclear, or perinuclear, respectively). (B) Quantitative analysis of the immunofluorescence pictures. AAV8-assembled particles were quantified in the intranuclear, perinuclear, and cytoplasmic cellular compartments at 1, 5, and 16 hr post-infection with AAV8-GFP (upper panel) or AAV8ΔVP1-GFP (lower panel). Results obtained with AAV8ΔVP1 and AAV8 were compared by a two-tailed Mann-Whitney test for each cell compartment. *p < 0.05, **p < 0.005, ***p ≤ 0.0001; N = total number of AAV8 particles counted at each time point. Data are presented as mean ± SD.

Article Snippet: Cells were then incubated with anti-mouse Alexa Fluor 555 secondary antibody (1:200 in PBS), washed with PBS, and incubated with DraQ5 (1:1,000 in PBS) for nuclei staining (Interchim, Montluçon, France).

Techniques: Immunofluorescence, Infection, Control, Staining, Two Tailed Test, MANN-WHITNEY

Journal: Physiological Genomics

Article Title: RNO3 QTL regulates vascular structure and arterial stiffness in the spontaneously hypertensive rat

doi: 10.1152/physiolgenomics.00038.2021

Figure Lengend Snippet: MPM imaging of arterial sections. SHR.BN3 ( A ) and SHR ( B ) arterial cross section at ×10 ( i ) and ×40 ( ii ) magnification. Nuclei are labeled with DRAQ5 (blue), elastin (green), and collagen (red). C : average number of nuclei in the adventitia and media of arteries in the SHR.BN3 ( n = 12) and SHR ( n = 17). D : quantification of collagen and collagen bundles in arteries. E : immunohistochemical detection and quantification of Ki-67 in formalin-fixed paraffin-embedded arterial cross sections of SHR.BN3 ( F ; n = 12) and SHR ( G ; n = 17). Slides were stained with DAB and hematoxylin (positive cells brown, nucleus blue). Scale bars equal 100 µm and 50 µm as indicated. Values are expressed as means ± SE. * P < 0.05, statistically significant. DAB, diaminobenzidine; DRAQ5, Deep Red Anthraquinone 5; Ki-67, nuclear protein Ki-67; MPM, multiphoton microscopy; SHR, spontaneously hypertensive rat.

Article Snippet: Using MetaMorph software, nuclei stained with DRAQ5 were counted at three locations within the tunica media and adventitia, and the counts were averaged.

Techniques: Imaging, Labeling, Immunohistochemical staining, Formalin-fixed Paraffin-Embedded, Staining, Microscopy