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    This is a recombinant monoclonal antibody This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format for improved compatibility with existing reagents assays
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    99
    Thermo Fisher er tracker blue white dpx
    Er Tracker Blue White Dpx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 860 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore dpx mounting medium
    Dpx Mounting Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1591 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore dpx mountant
    Dpx Mountant, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1347 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dpx  (Avantor)
    92
    Avantor dpx
    Dpx, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 801 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher dpx
    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from <t>HPTS/DPX</t> loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .
    Dpx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck & Co dpx
    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from <t>HPTS/DPX</t> loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .
    Dpx, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor dpx mounting medium
    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from <t>HPTS/DPX</t> loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .
    Dpx Mounting Medium, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dpx mounting medium - by Bioz Stars, 2021-01
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    92
    Merck KGaA dpx
    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from <t>HPTS/DPX</t> loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .
    Dpx, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific dpx
    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from <t>HPTS/DPX</t> loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .
    Dpx, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA dpx mounting medium
    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from <t>HPTS/DPX</t> loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .
    Dpx Mounting Medium, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA dpx mountant
    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from <t>HPTS/DPX</t> loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .
    Dpx Mountant, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cellpath dpx
    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from <t>HPTS/DPX</t> loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .
    Dpx, supplied by Cellpath, used in various techniques. Bioz Stars score: 92/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific dpx mounting medium
    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from <t>HPTS/DPX</t> loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .
    Dpx Mounting Medium, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dpx mounting medium - by Bioz Stars, 2021-01
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    92
    Fisher Scientific dpx mountant
    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from <t>HPTS/DPX</t> loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .
    Dpx Mountant, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agar Scientific dpx mounting medium
    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from <t>HPTS/DPX</t> loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .
    Dpx Mounting Medium, supplied by Agar Scientific, used in various techniques. Bioz Stars score: 92/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co dpx mounting medium
    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from <t>HPTS/DPX</t> loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .
    Dpx Mounting Medium, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore p xylene bis pyridinium bromide dpx permount
    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from <t>HPTS/DPX</t> loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .
    P Xylene Bis Pyridinium Bromide Dpx Permount, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Lys Arg 13C and 15N metabolically labeled recombinant human protein fragment
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    Image Search Results


    Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from HPTS/DPX loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .

    Journal: Nature Communications

    Article Title: The C. difficile toxin B membrane translocation machinery is an evolutionarily conserved protein delivery apparatus

    doi: 10.1038/s41467-020-14306-z

    Figure Lengend Snippet: Defining the evolutionarily conserved and minimal LCT translocase region. a Construct design of ADPR-[truncated TcdB T-domain]-DTR chimeras. b Dye release from HPTS/DPX loaded liposomes at pH 4.5, with truncated TcdB T-domains colored as follows: 800–1500 (red), 800–1473 (green), 800–1394 (yellow), and 800–1338 (blue). c Quantification of dye release from HPTS/DPX loaded liposomes after 20 min, at pH 4.5 and pH 8.0 ( N = 3). d Box plot of EC 50 values from cell viability and protein synthesis inhibition of Vero cells ( N = 4), where center line is the median, bounds of box represent the interquartile range, and the wiskers represent the minimum and maximum of data. Panels e – h the same as a – d , respectively, but with truncated TcdB T-domains 851–1500 (red), 851–1473 (green), 851–1394 (yellow), and 851–1338 (blue). Source data are provided as a Source Data File for Fig. 5 c , g , d , h .

    Article Snippet: After drying down with N2 , the lipid film was resuspended in 20 mM Tris, 150 mM NaCl, pH 8.0, 35 mM 8-Hydroxypyrene-1,3,6-trisulfonic acid (HPTS) and 50 mM p-xylene-bis-pyridinium bromide (DPX) (Thermo Fischer).

    Techniques: Construct, Inhibition

    TcdB 851-1473 is an autonomous protein domain. a TcdA T-domain (pdb: 4R04), with 851–1473 colored in green. b TcdB 851–1473 induced dye release from HPTS/DPX loaded liposomes from pH 4.0 to pH 7.0 in 0.5 pH increments. c Stability of TcdB 851–1473 in aqueous buffer and with d 20 mM DPC from pH 4.0 to pH 7.5 in 0.5 pH increments. e Circular dichroism (CD) spectroscopy of TcdB 851–1473 in aqueous buffer and f 20 mM DPC from pH 4.0 to pH 7.5 in 0.5 pH. Coloring for each pH in f is the same as in e . All experiments are N = 3. Source data are provided as a Source Data File for Fig. 7c, d.

    Journal: Nature Communications

    Article Title: The C. difficile toxin B membrane translocation machinery is an evolutionarily conserved protein delivery apparatus

    doi: 10.1038/s41467-020-14306-z

    Figure Lengend Snippet: TcdB 851-1473 is an autonomous protein domain. a TcdA T-domain (pdb: 4R04), with 851–1473 colored in green. b TcdB 851–1473 induced dye release from HPTS/DPX loaded liposomes from pH 4.0 to pH 7.0 in 0.5 pH increments. c Stability of TcdB 851–1473 in aqueous buffer and with d 20 mM DPC from pH 4.0 to pH 7.5 in 0.5 pH increments. e Circular dichroism (CD) spectroscopy of TcdB 851–1473 in aqueous buffer and f 20 mM DPC from pH 4.0 to pH 7.5 in 0.5 pH. Coloring for each pH in f is the same as in e . All experiments are N = 3. Source data are provided as a Source Data File for Fig. 7c, d.

    Article Snippet: After drying down with N2 , the lipid film was resuspended in 20 mM Tris, 150 mM NaCl, pH 8.0, 35 mM 8-Hydroxypyrene-1,3,6-trisulfonic acid (HPTS) and 50 mM p-xylene-bis-pyridinium bromide (DPX) (Thermo Fischer).

    Techniques: Spectroscopy

    Characterization of S. marcescens (Sm) toxin. a Light microscopy images of HeLa cells transfected with PiggyBac Dual Promoter PB5131B-1 vector, expressing empty plasmid or Sm toxin effector (1–600 or 36–600) after 24 h. b Addition of Insp6 (+) to Sm toxin results in cleavage of the effector (band 3) and the CPD (band 4) from the full-length toxin (band 1). Band 2 represents full-length Sm toxin without the effector and CPD. c Dye release from HPTS/DPX loaded liposomes at pH 4.5 and pH 8.0. d Quantification of dye release from HPTS/DPX loaded liposomes after 20 minutes. N = 3 for all experiments. Source data are provided as a Source Data File for Fig. 3e. e Light microscopy images of HCT-116 cells with no toxin (untreated) or with varying concentrations of Sm toxin after 24 h.

    Journal: Nature Communications

    Article Title: The C. difficile toxin B membrane translocation machinery is an evolutionarily conserved protein delivery apparatus

    doi: 10.1038/s41467-020-14306-z

    Figure Lengend Snippet: Characterization of S. marcescens (Sm) toxin. a Light microscopy images of HeLa cells transfected with PiggyBac Dual Promoter PB5131B-1 vector, expressing empty plasmid or Sm toxin effector (1–600 or 36–600) after 24 h. b Addition of Insp6 (+) to Sm toxin results in cleavage of the effector (band 3) and the CPD (band 4) from the full-length toxin (band 1). Band 2 represents full-length Sm toxin without the effector and CPD. c Dye release from HPTS/DPX loaded liposomes at pH 4.5 and pH 8.0. d Quantification of dye release from HPTS/DPX loaded liposomes after 20 minutes. N = 3 for all experiments. Source data are provided as a Source Data File for Fig. 3e. e Light microscopy images of HCT-116 cells with no toxin (untreated) or with varying concentrations of Sm toxin after 24 h.

    Article Snippet: After drying down with N2 , the lipid film was resuspended in 20 mM Tris, 150 mM NaCl, pH 8.0, 35 mM 8-Hydroxypyrene-1,3,6-trisulfonic acid (HPTS) and 50 mM p-xylene-bis-pyridinium bromide (DPX) (Thermo Fischer).

    Techniques: Light Microscopy, Transfection, Plasmid Preparation, Expressing