Journal: Virchows Archiv : an international journal of pathology

Article Title: Expression and clinical role of the dipeptidyl peptidases DPP8 and DPP9 in ovarian carcinoma.

doi: 10.1007/s00428-018-2487-x

Figure Lengend Snippet: Fig. 2 DPP8 and DPP9 protein expression in HGSC effusions. Cytoplasmic expression of DPP8 (a, b) and DPP9 (c, d) in tumor cells

Article Snippet: The DPP8 antibody was a mouse monoclonal antibody purchased from Novus Biologicals (cat no. NBP2-01830, clone OTI1D2; Littleton, CO), applied at a 1:200 dilution.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Inhibition of dipeptidyl peptidase 8/9 impairs preadipocyte differentiation

doi: 10.1038/srep12348

Figure Lengend Snippet: ( A ) Oil Red O staining of 3T3-L1 control cells (DMI) and cells treated with specific DPP inhibitors like DPP4 inhibitor MK-0431(DMI+ MK-0431), the DPP8/9 inhibitor 1G244 (DMI+1G244) and FAP inhibitor 3099 (DMI+3099) at day 8 of differentiation. ( B ) Adipocyte markers, FABP4, adiponectin (AdipoQ) and leptin were measured by real time PCR at day 8 of differentiation. β-actin expression was used as an internal control.

Article Snippet: For DPP8 or DPP9 knockdown: DPP8 shRNA plasmid (Origene, TF504979) was puromycin resistance, with sequence 5′ TTCCTGAGTCTGGAGAACACTATGAACTG-3′ ; DPP9 shRNA plasmid (Origene, TG515456) was puromycin resistance, with sequence 5′- TGTCAAGCTGCGAGAAGGAACTGGTACAG-3′.

Techniques: Staining, Control, Real-time Polymerase Chain Reaction, Expressing

Journal: Scientific Reports

Article Title: Inhibition of dipeptidyl peptidase 8/9 impairs preadipocyte differentiation

doi: 10.1038/srep12348

Figure Lengend Snippet: ( A ) Gene expression analysis of DPP8 and DPP9 during 3T3L1 differentiation. 3T3L1 cells were cultured in 10% FBS (NC) or treated with dexamethasone, isobutylmethylxanthine and insulin (DMI) from day 0 to day 2 of differentiation, mRNA levels of DPP8 and DPP9 were measured by real time PCR. β-actin expression was used as an internal control. ( B ) Oil Red O staining of control-shRNA-transduced cells (shCTL) and cells with DPP8/9 shRNAs (shDPP8, shDPP9) at day 8 of differentiation. ( C ) Adipocyte markers, FABP4, adiponectin (AdipoQ) and leptin, were measured by real time PCR at day 8 of differentiation. β-actin expression was used as an internal control.

Article Snippet: For DPP8 or DPP9 knockdown: DPP8 shRNA plasmid (Origene, TF504979) was puromycin resistance, with sequence 5′ TTCCTGAGTCTGGAGAACACTATGAACTG-3′ ; DPP9 shRNA plasmid (Origene, TG515456) was puromycin resistance, with sequence 5′- TGTCAAGCTGCGAGAAGGAACTGGTACAG-3′.

Techniques: Gene Expression, Cell Culture, Real-time Polymerase Chain Reaction, Expressing, Control, Staining, shRNA

Journal: Scientific Reports

Article Title: Inhibition of dipeptidyl peptidase 8/9 impairs preadipocyte differentiation

doi: 10.1038/srep12348

Figure Lengend Snippet: ( A ) PPARγ2, CEBPα, CEBPβ and CEBPδ mRNA levels were measured in control-shRNA-transduced 3T3-L1 cells (shCTL) and cells with DPP8/9 shRNAs (shDPP8, shDPP9) at basal level. ( B ) PPARγ2 and CEBPα mRNA levels were measured in control-shRNA-transduced cells (shCTL.) and cells with DPP8/9 shRNAs (shDPP8, shDPP9) after 48 hour treatment of DMI. CEBPβ and CEBPδ mRNA levels were measured after 6 hour treatment. ( C ) PPARγ2 and CEBPα mRNA levels were measured after 48 hour treatment of dexamethasone, isobutylmethylxanthine and insulin (DMI) with or without the DPP4 inhibitor MK-0431(DMI+MK-0431), the DPP8/9 inhibitor 1G244 (DMI+1G244), the FAP inhibitor 3099 (DMI+3099). CEBPβ and CEBPδ mRNA levels were measured in these cells after 6 hour treatment.

Article Snippet: For DPP8 or DPP9 knockdown: DPP8 shRNA plasmid (Origene, TF504979) was puromycin resistance, with sequence 5′ TTCCTGAGTCTGGAGAACACTATGAACTG-3′ ; DPP9 shRNA plasmid (Origene, TG515456) was puromycin resistance, with sequence 5′- TGTCAAGCTGCGAGAAGGAACTGGTACAG-3′.

Techniques: Control, shRNA

Journal: Scientific Reports

Article Title: Inhibition of dipeptidyl peptidase 8/9 impairs preadipocyte differentiation

doi: 10.1038/srep12348

Figure Lengend Snippet: ( A ) Representative western blot for the expression of PPARγ in stable 3T3-L1 cells transduced with control plasmid (vehicle) or PPARγ2 plasmid (PPARγ2). The blots were cropped, and the full-length blots are presented in the . ( B ) Oil Red O staining of control cells or PPARγ2 overexpressed 3T3-L1 cells, treated vehicle (NC), DMI (DMI), 500 μM non-selective DPP4 family inhibitor P32/98 (DMI+P32/98) or 20 μM DPP8/9 inhibitor 1G244 (DMI+1G244) at day 8 of differentiation. ( C ) Oil Red O staining of 3T3-L1 cells treated with 20μM DPP8/9 inhibitor 1G244 (DMI+1G244) or 1G244 plus 1 μM rosiglitazone(DMI+1G244+Rosi) or 5 μM troglitazone (DMI+1G244+Tro). ( D ) Adipocyte markers, FABP4, adiponectin (AdipoQ) and leptin, were measured in these cells by real time PCR at day 8 of differentiation. β-actin expression was used as an internal control.

Article Snippet: For DPP8 or DPP9 knockdown: DPP8 shRNA plasmid (Origene, TF504979) was puromycin resistance, with sequence 5′ TTCCTGAGTCTGGAGAACACTATGAACTG-3′ ; DPP9 shRNA plasmid (Origene, TG515456) was puromycin resistance, with sequence 5′- TGTCAAGCTGCGAGAAGGAACTGGTACAG-3′.

Techniques: Western Blot, Expressing, Transduction, Control, Plasmid Preparation, Staining, Real-time Polymerase Chain Reaction