Journal: Emerging Infectious Diseases

Article Title: Domestic Pig Unlikely Reservoir for MERS-CoV

doi: 10.3201/eid2306.170096

Figure Lengend Snippet: Comparison of the amino acid residues shown to be essential in binding of Middle East respiratory syndrome coronavirus spike protein to DPP4 of human, dromedary camel, and domestic pig*

Article Snippet: We investigated whether DPP4 is expressed in the pig respiratory tract by performing immunohistochemical staining on the nasal mucosa and lung tissue obtained from healthy pigs using an antibody against DPP4 (mouse monoclonal anti-DPP4 [CD26], clone OTI11D7, 1:2,500; Origene Technologies, Inc., Rockville, MD, USA).

Techniques: Comparison, Binding Assay

Journal: Emerging Infectious Diseases

Article Title: Domestic Pig Unlikely Reservoir for MERS-CoV

doi: 10.3201/eid2306.170096

Figure Lengend Snippet: Dipeptidyl peptidase (DPP) 4 expression in the domestic pig respiratory tract. Tissues were stained by using a cross-reactive mouse monoclonal antibody against DPP4 (CD26, clone OTI11D7, 1:2,500; Origene Technologies, Inc., Rockville, MD, USA). DPP4 expression was absent in the nasal mucosa (A) but present in lung tissue (B) of healthy domestic pigs. Original magnification: nasal mucosa ×40; lung ×200.

Article Snippet: We investigated whether DPP4 is expressed in the pig respiratory tract by performing immunohistochemical staining on the nasal mucosa and lung tissue obtained from healthy pigs using an antibody against DPP4 (mouse monoclonal anti-DPP4 [CD26], clone OTI11D7, 1:2,500; Origene Technologies, Inc., Rockville, MD, USA).

Techniques: Expressing, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Double immunofluorescent staining of human subcutaneous adipose tissue sections with antibodies against T-cadherin (green) and DPP4 (red); nuclei were counterstained with DAPI (blue). Images were acquired using a Zeiss LSM 780 confocal microscope and ZEN2010 software, shown at lower magnification (A) and higher magnification (B) . A thick arrow points to a group of cells expressing both T-cadherin and DPP4 in the interstitium; thin arrows mark cells expressing only T-cadherin; ovals encircle adipocytes. Scale bar 50 µm. (C) The table shows the percentage of T-cadherin–positive, DPP4 + cells and double-positive cells (DPP4 + /T-cadherin + ), quantified from adipose tissue sections of two healthy donors.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Staining, Microscopy, Software, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Light microscopy of MSCs (of the two to three passages) isolated from human subcutaneous adipose tissue of a healthy donor (A) and immunofluorescent staining with antibodies against T-cadherin (green) (B) . Arrows indicate cells with low or no T-cadherin expression, whereas cells exhibiting green fluorescence corresponding to T-cadherin are clearly visible. Scale bar, 50 µm. Light microscopy of human MSCs (C) and double immunofluorescent staining with antibodies against T-cadherin green, (E) and DPP4 red, (F) nuclei were counterstained with DAPI blue, (D) . Arrows in (C–F) indicate one and the same cell co-expressing T-cadherin and DPP4. Images were acquired using a Leica DMI 6000B microscope equipped with a Leica DFC7000T digital camera and LAS X software. Scale bar, 20 µm. (G) Representative flow cytometry plot showing T-cadherin and DPP4 distribution in cultured MSCs. The proportion of double-positive (DPP4 + /T-cadherin + ) cells was 30.4%; 6.15% expressed only T-cadherin, and 14% expressed only DPP4.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Light Microscopy, Isolation, Staining, Expressing, Fluorescence, Microscopy, Software, Flow Cytometry, Cell Culture

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Individual UMAP plots showing the expression levels and distribution of CDH13 (encoding T-cadherin) in control MSCs (A) and MSCs after 4 days of adipogenic induction (B) . UMAP plots demonstrating DPP4 expression in control MSCs (C) and MSCs after 4 days of adipogenic induction (D) . (E) RT-qPCR analysis of MSCs cultured in control medium or under adipogenic induction conditions showing the dynamics of T-cadherin mRNA expression. T-cadherin/ CDH13 expression decreased by day 4 in adipogenic medium and remained low through day 10. RT-qPCR data are shown as the mean ± SD. T-test. **р< 0.01 *p < 0.05 vs. control media in corresponding experimental day. Results are representative of three biologically independent experiments.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Control, Quantitative RT-PCR, Cell Culture

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Integrated object. (A) FeaturePlot–UMAP-plot showing principal distribution of CDH13 gene expression (encoding for T-cadherin) in the integrated object; CDH13 expressing cells corresponds to Cluster 3 (more than 1-fold change of the average expression level); (B) FeaturePlot–UMAP-plot showing principal distribution of DPP4 gene expression (encoding for T-cadherin) in the integrated object; DPP4 expressing cells correspond to Cluster 3 (more than 1-fold change of the average expression level) (C) DimPlot–Integrated object UMAP-clustering. Sample proportion diagrams depict the ratio between the cell counts in the control MSC sample (Salmon) and in the MSC sample (Iris blue) after a 4-day induction of adipogenic differentiation within the Clusters. (D) DimPlot–Integrated object grouped by samples. CDH13 expression in the control MSC sample (Salmon) and MSC sample (Iris blue) after a 4-day induction of adipogenic differentiation. Cluster 3 predominantly contains cells from the control sample.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Gene Expression, Expressing, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Integrated object. FeaturePlot. Each cluster is denoted by color. Cluster 0 (Salmon) primarily contains cells expressing fibroblast markers and genes responsible for cell cycle regulation. Cluster 1 (Khaki) encompasses cells expressing preadipocyte-specific genes, such as CEBPB , PPARγ, CD36 and markers of mature adipocytes ( ADIPOQ , Perilipin1 , Perilipin4 ). In Cluster 2 (green), cells predominantly express genes related to mitosis. Cluster 3 (Blue) contains cells of interest with high level of T-cadherin expression, as well as classical MSC markers ( CD90 , PDGFR ), Wnt signaling genes , and DPP4 . In a separate remote Cluster 4 (Magenta), besides CDH13 , cells express Nestin , a marker of neural crest cells, and CD36 , a marker of adipocyte progenitors.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Marker

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Split violin-plots showing the relative expression levels and distribution of CDH13 (A) and DPP4 (B) genes in the control MSC sample (Salmon) and MSC sample after a 4-day induction of adipogenic differentiation (Iris blue). The highest CDH13 expression was detected in Cluster 3 in MSCs of the control sample compared to MSCs after a 4-day adipogenic induction. Similarly, the highest expression of DPP4 was found in Cluster 3 in MSCs of the control sample. Split violin plots were generated using the R package Seurat and the function VlnPlot with the argument split.by = “sample”.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Control, Generated

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: (A) DimPlot– GSE182158 object UMAP-clustering; (B) 2 cluster manual cell type annotation, the red oval marks cluster 2; (C) FeaturePlot–UMAP-plot showing principal distribution of CDH13 gene expression in the GSE182158 object; (D) FeaturePlot–UMAP-plot showing principal distribution of DPP4 gene expression (encoding for T-cadherin) in the GSE182158 object.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Gene Expression

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Elevated DPP4 expression in MSCs after lentiviral transduction in T-cadherin-overexpressing cells was verified using RT-qPCR (A) and Western blot (B) . β-tubulin was used as the loading control for Western blot analysis. Representative results from one of two biologically independent RT-qPCR and eight Western blot experiments are shown. ANOVA with multiple comparisons, **p < 0.01.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Transduction, Quantitative RT-PCR, Western Blot, Control

Journal: Cell Reports Methods

Article Title: Identification of activity-based biomarkers for early-stage pancreatic tumors in blood using single-molecule enzyme activity screening

doi: 10.1016/j.crmeth.2023.100688

Figure Lengend Snippet: Development of PMAC as a core scaffold for the microdevice-based assay (A) Concept of single-molecule enzyme activity analysis using microfabricated chambered device. (B) The design of the microfabricated chambered device for single-molecule enzyme activity analysis. (C) Structure of PMAC-based fluorogenic probes of amidases, peptidases, and proteases. (D) Absorbance spectra of EP-PMAC (5 μM) before (black line) and after (red line) a reaction with recombinant DPP4 (300 ng/mL) in Tris-HCl buffer (pH 7.4, 100 mM) containing CHAPS (0.1%) at 25°C. (E) Fluorescence spectra of EP-PMAC in the same condition as in (d). Fluorescence spectra were measured every 10 min. λ ex. = 350 nm. (F) Structures of GP-AMC and EP-PMAC and their kinetic parameters in the reaction with DPP4 in 384-well plate-based assay. (G) Fluorescence images of microdevice loaded with GP-PMAC or EP-PMAC (50 μM) and recombinant DPP4 (10 ng/mL) in HEPES buffer (pH 7.4, 100 mM) containing CaCl 2 (1 mM), MgCl 2 (1 mM), DTT (1 mM), and Triton X-100 (3 mM). Images were taken after incubation at 37°C for 2 h.

Article Snippet: Anti-DPP4 (CD26) mouse monoclonal antibody , Origene , Cat. #TA500733 Lot #F002; RRID: AB_11140529.

Techniques: Activity Assay, Recombinant, Fluorescence, Incubation

Journal: Cell Reports Methods

Article Title: Identification of activity-based biomarkers for early-stage pancreatic tumors in blood using single-molecule enzyme activity screening

doi: 10.1016/j.crmeth.2023.100688

Figure Lengend Snippet: Identification of high- and low-activity species of single-molecule DPP4 and its alteration in blood samples of patients with pancreatic tumors (A) Fluorescence images of microdevice loaded with EP-PMAC (50 μM) and plasma samples (1:5,000 dilution; top) or recombinant DPP4 (1 ng/mL; bottom). The histograms indicate the activity distribution of single-molecule enzymes. White arrows indicate the low-activity DPP4 species detected in plasma. (B) Histograms of single-molecular activities of EP-PMAC (50 μM) with blood samples (1:5,000 dilution) of patients with early-stage pancreatic tumor (stages I and II, 30 individuals) and healthy subjects (30 individuals). Samples with aberrant ratio of high- and low-activity species of DPP4 were indicated by red square. (C) Left: dotted plot of the proportion of high-activity DPP4 species in plasma of early-stage (stages I and II) pancreatic tumor patients or of healthy subjects in (B). p value was calculated using Student’s t test. Right: dot plot of the total DPP4 activity in plasma samples monitored using conventional 384-well plate-based fluorometric assay. EP-PMAC (10 μM) was incubated with plasma samples (1:500 dilution) in HEPES buffer (pH 7.4, 100 mM) containing CaCl 2 (1 mM), MgCl 2 (1 mM), DTT (1 mM), and CHAPS (0.1%) at 25°C for 30 min. p value was calculated using Student’s t test. (D) Western blot analysis of DPP4 of plasma samples of patients with pancreatic tumors that showed altered DPP4 populations or that of healthy subjects.

Article Snippet: Anti-DPP4 (CD26) mouse monoclonal antibody , Origene , Cat. #TA500733 Lot #F002; RRID: AB_11140529.

Techniques: Activity Assay, Fluorescence, Clinical Proteomics, Recombinant, Incubation, Western Blot

Journal: Cell Reports Methods

Article Title: Identification of activity-based biomarkers for early-stage pancreatic tumors in blood using single-molecule enzyme activity screening

doi: 10.1016/j.crmeth.2023.100688

Figure Lengend Snippet: Decision tree analysis and receiver operating characteristic (ROC) curves in identification of early-stage (stages I and II) pancreatic tumor patients and healthy subjects by determining CD13 and DPP4 activities (A) ROC curves generated from dataset 1 (DS1; 30 patients with pancreatic tumors and 30 healthy subjects, blue line; Table S5 ) and dataset 2 (DS2; 30 patients with pancreatic tumors and 34 healthy subjects not included in DS1, red line; Table S6 ) are shown. (B) ROC curves generated by DPP4 activity, CD13 activity, and CA19-9 level in identification of pancreatic tumor patients (stages I and II) and healthy subjects for DS2. (C) ROC curves of activity-based biomarkers in the identification of pancreatic tumor patients. Left: ROC curves of the set of plasma samples in DS1. Right: ROC curves of the set of plasma samples in DS2. (D) Decision tree analysis of the first and second sets of plasma samples. (E) Decision tree analysis of the second set of plasma samples in combination with CA19-9. The analysis was also performed with the data after shuffling the label of CA19-9 or that of CD13 + DPP4.

Article Snippet: Anti-DPP4 (CD26) mouse monoclonal antibody , Origene , Cat. #TA500733 Lot #F002; RRID: AB_11140529.

Techniques: Generated, Activity Assay, Clinical Proteomics

Journal: Cell Reports Methods

Article Title: Identification of activity-based biomarkers for early-stage pancreatic tumors in blood using single-molecule enzyme activity screening

doi: 10.1016/j.crmeth.2023.100688

Figure Lengend Snippet:

Article Snippet: Anti-DPP4 (CD26) mouse monoclonal antibody , Origene , Cat. #TA500733 Lot #F002; RRID: AB_11140529.

Techniques: Recombinant, Clear Native PAGE, Electrophoresis, Purification

Journal: STAR Protocols

Article Title: Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

doi: 10.1016/j.xpro.2022.101951

Figure Lengend Snippet: Panel for flow cytometry analysis of sorted populations

Article Snippet: CD26 Antibody, anti-mouse, REAfinity- PE (1:100 dilution) , Miltenyi Biotec , Cat# 130-122-775, RRID: AB_2801934.

Techniques: Flow Cytometry, Marker, Staining

Journal: STAR Protocols

Article Title: Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

doi: 10.1016/j.xpro.2022.101951

Figure Lengend Snippet: Sample detail for fluorescence compensation settings

Article Snippet: CD26 Antibody, anti-mouse, REAfinity- PE (1:100 dilution) , Miltenyi Biotec , Cat# 130-122-775, RRID: AB_2801934.

Techniques: Fluorescence, Staining, Suspension

Journal: STAR Protocols

Article Title: Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

doi: 10.1016/j.xpro.2022.101951

Figure Lengend Snippet: Flow cytometry gating and isolated fraction purity/viability (A) Gating strategy for cellular subsets identification. Singlet live cells were obtained by using SSC/Time, FSC/SSC and FSC/mortality dye (LD) parameters. (B) To appreciate each fraction purity, dot plot with CD45, CD31, Lin and CD26 (CAFs marker) staining were shown in CD45+ TILs, tumor cells and CAFs in total tumor suspension. (C) Proportion of CD45+ TILs, Endothelial cells, Tumor cells and CAFs in tumor cell suspension and purity of each cell subsets in isolated fractions.

Article Snippet: CD26 Antibody, anti-mouse, REAfinity- PE (1:100 dilution) , Miltenyi Biotec , Cat# 130-122-775, RRID: AB_2801934.

Techniques: Flow Cytometry, Isolation, Marker, Staining, Suspension

Journal: STAR Protocols

Article Title: Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

doi: 10.1016/j.xpro.2022.101951

Figure Lengend Snippet: Phenotypic and transcriptomic analysis (A and B) Representative flow cytometry analysis of CAFs markers (CD26, FAPa, PDPN, PDGFRa, PDGFRb) on tumor cells and CAFs fractions (A). Normalized expression of each marker (B). (C) Analysis of CAFs, CD45+ TILs and tumor associated genes expression by RT-qPCR in each isolated fraction (n = 3 samples/fraction).

Article Snippet: CD26 Antibody, anti-mouse, REAfinity- PE (1:100 dilution) , Miltenyi Biotec , Cat# 130-122-775, RRID: AB_2801934.

Techniques: Flow Cytometry, Expressing, Marker, Quantitative RT-PCR, Isolation

Journal: STAR Protocols

Article Title: Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

doi: 10.1016/j.xpro.2022.101951

Figure Lengend Snippet:

Article Snippet: CD26 Antibody, anti-mouse, REAfinity- PE (1:100 dilution) , Miltenyi Biotec , Cat# 130-122-775, RRID: AB_2801934.

Techniques: Recombinant, Flow Cytometry, Staining, Isolation, Cell Isolation, Software, Real-time Polymerase Chain Reaction, Blocking Assay, Gentle