dpbs Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Thermo Fisher dpbs powder
    Dpbs Powder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs powder/product/Thermo Fisher
    Average 94 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    dpbs powder - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    94
    Thermo Fisher dpbs
    Brightfield DIC microscopy images of (A) Oleo30G_S2C, (B) Oleo30G_T3C, (C) Oleo30G_T4C, (D) Oleo30G_T5C, (E) Oleo30G_T12C, (F) Oleo30G_T24C, (G) Oleo30G_S39C, and (H) Oleo30G. Protein solutions were at a concentration of 80 μM protein in <t>DPBS</t> with 1 mM <t>DTT.</t> Samples were chilled on ice for 10 minutes before transferring to chambered coverglass coated with pluronic F-127. As the droplets are denser than water, they fell to the bottom of the chamber. Images were taken at the coverglass. Scale bar = 20 μm
    Dpbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 14524 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Thermo Fisher
    Average 94 stars, based on 14524 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    93
    Lonza magnesium free dpbs dpbs
    CFHR5 competes with FH in serum for binding to PTX3, <t>CRP,</t> and MaxGel and causes enhanced C3 fragment deposition. ( A ) Wells were coated with 10 μg/ml PTX3, 10 μg/ml CRP, and MaxGel diluted 1:30 in <t>DPBS.</t> After blocking, the wells were incubated for 30 min at 37°C with 25% heat-inactivated human serum with or without 0.5 μM CFHR5 and CFHR4A. FH binding was detected with mAb A254 and the corresponding secondary Ab. The normalized values are means ± SD derived from three independent experiments. *** p
    Magnesium Free Dpbs Dpbs, supplied by Lonza, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnesium free dpbs dpbs/product/Lonza
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    magnesium free dpbs dpbs - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    96
    Thermo Fisher magnesium dpbs
    CFHR5 competes with FH in serum for binding to PTX3, <t>CRP,</t> and MaxGel and causes enhanced C3 fragment deposition. ( A ) Wells were coated with 10 μg/ml PTX3, 10 μg/ml CRP, and MaxGel diluted 1:30 in <t>DPBS.</t> After blocking, the wells were incubated for 30 min at 37°C with 25% heat-inactivated human serum with or without 0.5 μM CFHR5 and CFHR4A. FH binding was detected with mAb A254 and the corresponding secondary Ab. The normalized values are means ± SD derived from three independent experiments. *** p
    Magnesium Dpbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnesium dpbs/product/Thermo Fisher
    Average 96 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    magnesium dpbs - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    94
    Becton Dickinson dpbs
    Comparison of <t>TrypLE™</t> Select animal-free tyrpsin immobilization and cold PBS with cell scraping for harvesting mature HOCl-oxidized lysate-loaded DCs . (A) DCs were matured with LPS and IFN-γ for 16 h following lysate-loading. It was observed that mature lysate-loaded DCs that were harvested using TrypLE™ Select animal-free tyrpsin replacement exhibited approximately 30-40% reduction in the levels of MHC Class I ( P = 0.13; NS) and HLA-DR ( P = 0.15; NS) compared to DCs that were harvested with cold <t>DPBS</t> and cell scraping. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) ± SEM in research-scale Nunclon™Δ Surface T25 cm 2 flasks. P values were determined with unpaired Student's t test. NS indicated that P values were not significant. (B) Mature lysate-loaded DCs that were harvested with TrypLE™ Select animal-free tyrpsin replacement were as efficient as DCs that were harvested with cold DPBS and cell scraping in presenting MHC Class I-restricted HER-2/neu and MART-1 peptides and stimulating HER-2/neu and MART-1 CD8 + T cells in intracellular IFN-γ assessments.
    Dpbs, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 2962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Becton Dickinson
    Average 94 stars, based on 2962 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    92
    Biological Industries Inc dpbs
    Comparison of <t>TrypLE™</t> Select animal-free tyrpsin immobilization and cold PBS with cell scraping for harvesting mature HOCl-oxidized lysate-loaded DCs . (A) DCs were matured with LPS and IFN-γ for 16 h following lysate-loading. It was observed that mature lysate-loaded DCs that were harvested using TrypLE™ Select animal-free tyrpsin replacement exhibited approximately 30-40% reduction in the levels of MHC Class I ( P = 0.13; NS) and HLA-DR ( P = 0.15; NS) compared to DCs that were harvested with cold <t>DPBS</t> and cell scraping. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) ± SEM in research-scale Nunclon™Δ Surface T25 cm 2 flasks. P values were determined with unpaired Student's t test. NS indicated that P values were not significant. (B) Mature lysate-loaded DCs that were harvested with TrypLE™ Select animal-free tyrpsin replacement were as efficient as DCs that were harvested with cold DPBS and cell scraping in presenting MHC Class I-restricted HER-2/neu and MART-1 peptides and stimulating HER-2/neu and MART-1 CD8 + T cells in intracellular IFN-γ assessments.
    Dpbs, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Biological Industries Inc
    Average 92 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Cambrex dpbs
    Comparison of <t>TrypLE™</t> Select animal-free tyrpsin immobilization and cold PBS with cell scraping for harvesting mature HOCl-oxidized lysate-loaded DCs . (A) DCs were matured with LPS and IFN-γ for 16 h following lysate-loading. It was observed that mature lysate-loaded DCs that were harvested using TrypLE™ Select animal-free tyrpsin replacement exhibited approximately 30-40% reduction in the levels of MHC Class I ( P = 0.13; NS) and HLA-DR ( P = 0.15; NS) compared to DCs that were harvested with cold <t>DPBS</t> and cell scraping. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) ± SEM in research-scale Nunclon™Δ Surface T25 cm 2 flasks. P values were determined with unpaired Student's t test. NS indicated that P values were not significant. (B) Mature lysate-loaded DCs that were harvested with TrypLE™ Select animal-free tyrpsin replacement were as efficient as DCs that were harvested with cold DPBS and cell scraping in presenting MHC Class I-restricted HER-2/neu and MART-1 peptides and stimulating HER-2/neu and MART-1 CD8 + T cells in intracellular IFN-γ assessments.
    Dpbs, supplied by Cambrex, used in various techniques. Bioz Stars score: 92/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Cambrex
    Average 92 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Cellgro dpbs
    Comparison of <t>TrypLE™</t> Select animal-free tyrpsin immobilization and cold PBS with cell scraping for harvesting mature HOCl-oxidized lysate-loaded DCs . (A) DCs were matured with LPS and IFN-γ for 16 h following lysate-loading. It was observed that mature lysate-loaded DCs that were harvested using TrypLE™ Select animal-free tyrpsin replacement exhibited approximately 30-40% reduction in the levels of MHC Class I ( P = 0.13; NS) and HLA-DR ( P = 0.15; NS) compared to DCs that were harvested with cold <t>DPBS</t> and cell scraping. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) ± SEM in research-scale Nunclon™Δ Surface T25 cm 2 flasks. P values were determined with unpaired Student's t test. NS indicated that P values were not significant. (B) Mature lysate-loaded DCs that were harvested with TrypLE™ Select animal-free tyrpsin replacement were as efficient as DCs that were harvested with cold DPBS and cell scraping in presenting MHC Class I-restricted HER-2/neu and MART-1 peptides and stimulating HER-2/neu and MART-1 CD8 + T cells in intracellular IFN-γ assessments.
    Dpbs, supplied by Cellgro, used in various techniques. Bioz Stars score: 92/100, based on 315 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Cellgro
    Average 92 stars, based on 315 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Corning Life Sciences dpbs
    Urease has no effect on the survival of C . neoformans and killing of A . <t>castellanii</t> during co-incubation of C . neoformans and A . castellanii . A . castellanii (AC) was co-cultured with H99, ure1 Δ or ure1 Δ:: URE1 strain in <t>DPBS</t> with or without 7.5 mM urea. (A) The viability of A . castellanii was determined by Trypan Blue exclusion assay. The percentage of dead A . castellanii is determine by counting the number of Trypan Blue staining cells per total cell number counted. Two independent biological experiments were performed. Error bars are SD. P values shown in graph was calculated by Fisher’s exact test when each strain was compared with PBS alone at 48 h co-incubation. P > 0.05 when H99 and ure1 Δ:: URE1 were compared with ure1 Δ at 24 and 48 h by Fisher’s exact test. (B) The survival of cryptococcal strains were determined by colony form unit (CFU) after 0, 24 and 48 h. Two independent biological experiments were performed. Error bars are standard deviation (SD). P > 0.05 by Student’s t test.
    Dpbs, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 406 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Corning Life Sciences
    Average 92 stars, based on 406 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    EuroClone dpbs
    Urease has no effect on the survival of C . neoformans and killing of A . <t>castellanii</t> during co-incubation of C . neoformans and A . castellanii . A . castellanii (AC) was co-cultured with H99, ure1 Δ or ure1 Δ:: URE1 strain in <t>DPBS</t> with or without 7.5 mM urea. (A) The viability of A . castellanii was determined by Trypan Blue exclusion assay. The percentage of dead A . castellanii is determine by counting the number of Trypan Blue staining cells per total cell number counted. Two independent biological experiments were performed. Error bars are SD. P values shown in graph was calculated by Fisher’s exact test when each strain was compared with PBS alone at 48 h co-incubation. P > 0.05 when H99 and ure1 Δ:: URE1 were compared with ure1 Δ at 24 and 48 h by Fisher’s exact test. (B) The survival of cryptococcal strains were determined by colony form unit (CFU) after 0, 24 and 48 h. Two independent biological experiments were performed. Error bars are standard deviation (SD). P > 0.05 by Student’s t test.
    Dpbs, supplied by EuroClone, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/EuroClone
    Average 91 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    93
    GE Healthcare dpbs
    <t>Lhx8</t> knockdown accelerates mesenchyme differentiation ( A ) Schematic representation of the Lhx8 intervention and dental epithelium and dental mesenchyme recombination procedures. Dental epithelium and mesenchyme of mandibular first molar at E16.5 are separated and then the dental mesenchyme is placed onto upper chamber of Transwell cell culture insert and further incubated with either RNAi or lentivirus for 1 hr at RT. Meanwhile the dental epithelium is kept in ice cold <t>DPBS.</t> Then the mesenchyme and epithelium are recombined and cultured for up to 7 days. ( B and C ) Representative data showing the location ( B ) and morphology ( C ) of the tooth germ of E16.5 mandibular first molar. ( D–F ) The top view of the recombined tooth germ right after recombination ( D ), 1 day culture ( E ), 3 days culture ( F ) and 7 days culture ( G, control group; K , siLhx8 group). ( H–N ) The recombined tooth germs from control ( G–J ) and siLhx8 (K–N) were harvested at day 7 and chemically stained with HE ( H , I , L and M ) and immunostained with Dspp ( J , N ) ( n=5) . ( O–Q ) Immunofluorescence of the isolated E16.5 dental mesenchyme transfected with Lipofectamine RNAiMAX coated Cy3 labeled RNAi duplex before recombination. Strong positive Cy3 dots indicate high transfection efficiency. ( R ) Knockdown efficiency of Lhx8. ( S , T ) Quantification data of dentin area ( S ) and thickness ( T ). ( U ) Tooth development related gene expression were examined by qPCR in the recombined tooth germs 4 days after in vitro culture from both control and siLhx8 groups. Data presented are a representative of 3 experiments. de, dental epithelium; dm, dental mesenchyme; d, dentin; od, odontoblast; am, ameloblast; e, enamel. Scale bars: 1mm ( B–F, Q and K, O–Q) ; 500 μm ( I and M ); 250 μm ( J and N ); Data represent mean ± SD. * P
    Dpbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/GE Healthcare
    Average 93 stars, based on 773 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    93
    Mediatech dpbs
    Caco-2 cells were cultured in a 12 well plate until confluency. Fluorescently labeled <t>microparticles</t> suspended in <t>DPBS</t> were incubated with the Caco-2 cells for 30 minutes. After incubation, wells were washed (□) twice with HBSS to remove non-adherent
    Dpbs, supplied by Mediatech, used in various techniques. Bioz Stars score: 93/100, based on 442 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Mediatech
    Average 93 stars, based on 442 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    94
    Millipore dpbs
    Complement-mediated bacteriolysis activity of sera from mice inoculated with I-M28-47 and I-M28-47-114 against two different MDR A. <t>baumannii</t> growth conditions. The lysis activity percentages were determined using sera from mice inoculated with I-M28-47-114, I-M28-47 (control) or <t>DPBS</t> (placebo control) in presence of baby rabbit complement against MDR A. baumannii ( a ) cultured without imipenem treatment or ( b ) treated with 32 mg/L imipenem. The values are the means ± S.D. tested in duplicate. * P
    Dpbs, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 8108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Millipore
    Average 94 stars, based on 8108 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    92
    PAN - Biotech dpbs
    Complement-mediated bacteriolysis activity of sera from mice inoculated with I-M28-47 and I-M28-47-114 against two different MDR A. <t>baumannii</t> growth conditions. The lysis activity percentages were determined using sera from mice inoculated with I-M28-47-114, I-M28-47 (control) or <t>DPBS</t> (placebo control) in presence of baby rabbit complement against MDR A. baumannii ( a ) cultured without imipenem treatment or ( b ) treated with 32 mg/L imipenem. The values are the means ± S.D. tested in duplicate. * P
    Dpbs, supplied by PAN - Biotech, used in various techniques. Bioz Stars score: 92/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/PAN - Biotech
    Average 92 stars, based on 147 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    93
    Welgene inc dpbs
    The anticancer effects of <t>PGNO-media</t> in vivo . (a) Simple scheme of animal experiment. (b) Pictures of 12 mice with melanoma syngeneic mice after 11 days of daily administration of <t>DPBS</t> or PGNO-media. Arrows mark the positions of tumor. (c) Pictures of tumor tissue from each mice at day 12. (d) A table showing the volume of tumor tissue from each mice and the average and standard deviation for the two groups.
    Dpbs, supplied by Welgene inc, used in various techniques. Bioz Stars score: 93/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Welgene inc
    Average 93 stars, based on 147 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Biowest SAS dpbs
    The anticancer effects of <t>PGNO-media</t> in vivo . (a) Simple scheme of animal experiment. (b) Pictures of 12 mice with melanoma syngeneic mice after 11 days of daily administration of <t>DPBS</t> or PGNO-media. Arrows mark the positions of tumor. (c) Pictures of tumor tissue from each mice at day 12. (d) A table showing the volume of tumor tissue from each mice and the average and standard deviation for the two groups.
    Dpbs, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Biowest SAS
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Sangon Biotech dpbs
    The anticancer effects of <t>PGNO-media</t> in vivo . (a) Simple scheme of animal experiment. (b) Pictures of 12 mice with melanoma syngeneic mice after 11 days of daily administration of <t>DPBS</t> or PGNO-media. Arrows mark the positions of tumor. (c) Pictures of tumor tissue from each mice at day 12. (d) A table showing the volume of tumor tissue from each mice and the average and standard deviation for the two groups.
    Dpbs, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Sangon Biotech
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    dpbs  (Sony)
    92
    Sony dpbs
    The anticancer effects of <t>PGNO-media</t> in vivo . (a) Simple scheme of animal experiment. (b) Pictures of 12 mice with melanoma syngeneic mice after 11 days of daily administration of <t>DPBS</t> or PGNO-media. Arrows mark the positions of tumor. (c) Pictures of tumor tissue from each mice at day 12. (d) A table showing the volume of tumor tissue from each mice and the average and standard deviation for the two groups.
    Dpbs, supplied by Sony, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Sony
    Average 92 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    93
    Biochrom dpbs
    The anticancer effects of <t>PGNO-media</t> in vivo . (a) Simple scheme of animal experiment. (b) Pictures of 12 mice with melanoma syngeneic mice after 11 days of daily administration of <t>DPBS</t> or PGNO-media. Arrows mark the positions of tumor. (c) Pictures of tumor tissue from each mice at day 12. (d) A table showing the volume of tumor tissue from each mice and the average and standard deviation for the two groups.
    Dpbs, supplied by Biochrom, used in various techniques. Bioz Stars score: 93/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Biochrom
    Average 93 stars, based on 114 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Fisher Scientific dpbs
    The anticancer effects of <t>PGNO-media</t> in vivo . (a) Simple scheme of animal experiment. (b) Pictures of 12 mice with melanoma syngeneic mice after 11 days of daily administration of <t>DPBS</t> or PGNO-media. Arrows mark the positions of tumor. (c) Pictures of tumor tissue from each mice at day 12. (d) A table showing the volume of tumor tissue from each mice and the average and standard deviation for the two groups.
    Dpbs, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Fisher Scientific
    Average 92 stars, based on 188 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Regis Technologies dpbs
    The anticancer effects of <t>PGNO-media</t> in vivo . (a) Simple scheme of animal experiment. (b) Pictures of 12 mice with melanoma syngeneic mice after 11 days of daily administration of <t>DPBS</t> or PGNO-media. Arrows mark the positions of tumor. (c) Pictures of tumor tissue from each mice at day 12. (d) A table showing the volume of tumor tissue from each mice and the average and standard deviation for the two groups.
    Dpbs, supplied by Regis Technologies, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Regis Technologies
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    XBiotech dpbs
    The anticancer effects of <t>PGNO-media</t> in vivo . (a) Simple scheme of animal experiment. (b) Pictures of 12 mice with melanoma syngeneic mice after 11 days of daily administration of <t>DPBS</t> or PGNO-media. Arrows mark the positions of tumor. (c) Pictures of tumor tissue from each mice at day 12. (d) A table showing the volume of tumor tissue from each mice and the average and standard deviation for the two groups.
    Dpbs, supplied by XBiotech, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/XBiotech
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Caliper Life Sciences dpbs
    The anticancer effects of <t>PGNO-media</t> in vivo . (a) Simple scheme of animal experiment. (b) Pictures of 12 mice with melanoma syngeneic mice after 11 days of daily administration of <t>DPBS</t> or PGNO-media. Arrows mark the positions of tumor. (c) Pictures of tumor tissue from each mice at day 12. (d) A table showing the volume of tumor tissue from each mice and the average and standard deviation for the two groups.
    Dpbs, supplied by Caliper Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Caliper Life Sciences
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Brightfield DIC microscopy images of (A) Oleo30G_S2C, (B) Oleo30G_T3C, (C) Oleo30G_T4C, (D) Oleo30G_T5C, (E) Oleo30G_T12C, (F) Oleo30G_T24C, (G) Oleo30G_S39C, and (H) Oleo30G. Protein solutions were at a concentration of 80 μM protein in DPBS with 1 mM DTT. Samples were chilled on ice for 10 minutes before transferring to chambered coverglass coated with pluronic F-127. As the droplets are denser than water, they fell to the bottom of the chamber. Images were taken at the coverglass. Scale bar = 20 μm

    Journal: Soft matter

    Article Title: Redox Sensitive Protein Droplets from Recombinant Oleosin

    doi: 10.1039/c8sm01047a

    Figure Lengend Snippet: Brightfield DIC microscopy images of (A) Oleo30G_S2C, (B) Oleo30G_T3C, (C) Oleo30G_T4C, (D) Oleo30G_T5C, (E) Oleo30G_T12C, (F) Oleo30G_T24C, (G) Oleo30G_S39C, and (H) Oleo30G. Protein solutions were at a concentration of 80 μM protein in DPBS with 1 mM DTT. Samples were chilled on ice for 10 minutes before transferring to chambered coverglass coated with pluronic F-127. As the droplets are denser than water, they fell to the bottom of the chamber. Images were taken at the coverglass. Scale bar = 20 μm

    Article Snippet: Protein aliquots were thawed and dialyzed into DPBS (Thermo Fisher Scientific) with 1 mM DTT.

    Techniques: Microscopy, Concentration Assay, Transferring

    (A) UV-vis spectroscopy traces of Oleo30G and Oleo30G-cys mutants. Protein solutions were at a concentration of 80 μM protein in DPBS with 1 mM DTT. Measurements were taken starting at 37 °C and cooling at a rate of 1 °C per minute. Measurements were taken in increments if 0.5 °C. An increase in the absorbance indicates the formation of droplets. For clarity, data is shown only for 20 °C and below. (B) Temperature at which the absorbance measured by UV-vis spectroscopy while cooling at rate of 1 °C per minute reached 0.2 (a.u.) plotted against the position of the added cysteine. Scale bars show the standard deviation from three independent measurements. The transition temperature drops for variants with the cysteine further from the N-terminus of the protein. No values are shown for Oleo30G or Oleo30G_S39C because the absorbance did not reach 0.2.

    Journal: Soft matter

    Article Title: Redox Sensitive Protein Droplets from Recombinant Oleosin

    doi: 10.1039/c8sm01047a

    Figure Lengend Snippet: (A) UV-vis spectroscopy traces of Oleo30G and Oleo30G-cys mutants. Protein solutions were at a concentration of 80 μM protein in DPBS with 1 mM DTT. Measurements were taken starting at 37 °C and cooling at a rate of 1 °C per minute. Measurements were taken in increments if 0.5 °C. An increase in the absorbance indicates the formation of droplets. For clarity, data is shown only for 20 °C and below. (B) Temperature at which the absorbance measured by UV-vis spectroscopy while cooling at rate of 1 °C per minute reached 0.2 (a.u.) plotted against the position of the added cysteine. Scale bars show the standard deviation from three independent measurements. The transition temperature drops for variants with the cysteine further from the N-terminus of the protein. No values are shown for Oleo30G or Oleo30G_S39C because the absorbance did not reach 0.2.

    Article Snippet: Protein aliquots were thawed and dialyzed into DPBS (Thermo Fisher Scientific) with 1 mM DTT.

    Techniques: UV-Vis Spectroscopy, Concentration Assay, Standard Deviation

    (A) Brightfield DIC microscopy images of Oleo30G_S2C shows spherical droplets. The sample was at a concentration of 80 μM protein in DPBS with 1 mM DTT. The sample was chilled on ice for 10 minutes before transferring to chambered covergla0073s coated with pluronic F-127. Because the droplets are denser than water, they fell to the bottom of the chamber. Image was taken at the coverglass after droplets settled at the bottom. Scale bar = 20 μm. (B) Time-lapse images of Oleo30G_S2C shows the fusion of two droplets over the course of several minutes. Time starts after droplets settled onto the coverglass. Scale bar = 5 μm. (C) UV-vis spectroscopy traces of Oleo30G_S2C. The sample was at a concentration of 80 μM protein in DPBS with 1 mM DTT. Cooling trace was taken starting at 37 °C and cooling at a rate of 1 °C per minute. An increase in the absorbance indicates the formation of droplets. Heating trace was taken starting at 0 °C and heating at a rate of 1 °C per minute. Return of absorbance to near zero indicates return to a single phase. Measurements were taken in increments if 0.5 °C. (D) UV-vis spectroscopy traces of Oleo30G_S2C at various salt concentrations. The sample was at a concentration of 80 μM protein, 1mM DTT, and salt concentrations of 280 mM, 140 mM, and 70 mM NaCl. Measurements were taken starting at 50 °C and cooling at a rate of 1 °C per minute. Measurements were taken in increments if 0.5 °C.

    Journal: Soft matter

    Article Title: Redox Sensitive Protein Droplets from Recombinant Oleosin

    doi: 10.1039/c8sm01047a

    Figure Lengend Snippet: (A) Brightfield DIC microscopy images of Oleo30G_S2C shows spherical droplets. The sample was at a concentration of 80 μM protein in DPBS with 1 mM DTT. The sample was chilled on ice for 10 minutes before transferring to chambered covergla0073s coated with pluronic F-127. Because the droplets are denser than water, they fell to the bottom of the chamber. Image was taken at the coverglass after droplets settled at the bottom. Scale bar = 20 μm. (B) Time-lapse images of Oleo30G_S2C shows the fusion of two droplets over the course of several minutes. Time starts after droplets settled onto the coverglass. Scale bar = 5 μm. (C) UV-vis spectroscopy traces of Oleo30G_S2C. The sample was at a concentration of 80 μM protein in DPBS with 1 mM DTT. Cooling trace was taken starting at 37 °C and cooling at a rate of 1 °C per minute. An increase in the absorbance indicates the formation of droplets. Heating trace was taken starting at 0 °C and heating at a rate of 1 °C per minute. Return of absorbance to near zero indicates return to a single phase. Measurements were taken in increments if 0.5 °C. (D) UV-vis spectroscopy traces of Oleo30G_S2C at various salt concentrations. The sample was at a concentration of 80 μM protein, 1mM DTT, and salt concentrations of 280 mM, 140 mM, and 70 mM NaCl. Measurements were taken starting at 50 °C and cooling at a rate of 1 °C per minute. Measurements were taken in increments if 0.5 °C.

    Article Snippet: Protein aliquots were thawed and dialyzed into DPBS (Thermo Fisher Scientific) with 1 mM DTT.

    Techniques: Microscopy, Concentration Assay, Transferring, UV-Vis Spectroscopy

    (A) Brightfield DIC microscopy image of Oleo30G_S2C at a concentration of 80 μM protein in DPBS with 1 mM DTT. (B) Brightfield DIC microscopy image of Oleo30G_S2C after the addition of βME to a final concentration of 80 mM. Samples were chilled on ice for 10 minutes before imaging. Scale bar = 20 μm. (C) UV-vis spectroscopy traces of Oleo30G_S2C. The protein solution was at a concentration of 80 μM protein in DPBS with 1 mM DTT (blue curve). Measurements were taken in increments if 0.5 °C starting at 37 °C and cooling at a rate of 1 °C per minute. Measurements were taken again after addition of βME to a final concentration of 80 mM (grey curve). For clarity, data is shown only for 25 °C and below. (D) UV-vis spectroscopy traces of Oleo30G_S2C/T5C. Addition of βME reduced the phase transition temperature of the protein. Unlike the Oleo30G variants containing only a single cysteine, the double cysteine mutant had a measurable increase in turbidity upon cooling after the addition of βME. Solutions were at a concentration of 80 μM protein in DPBS with 1 mM DTT. Measurements were taken starting at 37 °C and cooling at a rate of 1 °C per minute to a final temperature of 0 °C. Measurements were taken in increments if 0.5 °C. For clarity, data is shown only for 25 °C and below.

    Journal: Soft matter

    Article Title: Redox Sensitive Protein Droplets from Recombinant Oleosin

    doi: 10.1039/c8sm01047a

    Figure Lengend Snippet: (A) Brightfield DIC microscopy image of Oleo30G_S2C at a concentration of 80 μM protein in DPBS with 1 mM DTT. (B) Brightfield DIC microscopy image of Oleo30G_S2C after the addition of βME to a final concentration of 80 mM. Samples were chilled on ice for 10 minutes before imaging. Scale bar = 20 μm. (C) UV-vis spectroscopy traces of Oleo30G_S2C. The protein solution was at a concentration of 80 μM protein in DPBS with 1 mM DTT (blue curve). Measurements were taken in increments if 0.5 °C starting at 37 °C and cooling at a rate of 1 °C per minute. Measurements were taken again after addition of βME to a final concentration of 80 mM (grey curve). For clarity, data is shown only for 25 °C and below. (D) UV-vis spectroscopy traces of Oleo30G_S2C/T5C. Addition of βME reduced the phase transition temperature of the protein. Unlike the Oleo30G variants containing only a single cysteine, the double cysteine mutant had a measurable increase in turbidity upon cooling after the addition of βME. Solutions were at a concentration of 80 μM protein in DPBS with 1 mM DTT. Measurements were taken starting at 37 °C and cooling at a rate of 1 °C per minute to a final temperature of 0 °C. Measurements were taken in increments if 0.5 °C. For clarity, data is shown only for 25 °C and below.

    Article Snippet: Protein aliquots were thawed and dialyzed into DPBS (Thermo Fisher Scientific) with 1 mM DTT.

    Techniques: Microscopy, Concentration Assay, Imaging, UV-Vis Spectroscopy, Sublimation, Mutagenesis

    (A) UV-vis spectroscopy traces of Oleo30G_S2C, Oleo30G, and Oleo30G_S2C/Oleo30G blends. Protein solutions were at a concentration of 80 μM protein in DPBS with 1 mM DTT. Blends had a total protein concentration of 80 μM. Blend molar ratios used were 75%/25%, 50%/50%, and 25%/75% Oleo30G_S2C/Oleo30G. Measurements were taken starting at 37 °C and cooling at a rate of 1 °C per minute. Measurements were taken in increments if 0.5 °C. An increase in the absorbance indicates the formation of droplets. For clarity, data is shown only for 20 °C and below. Brightfield DIC microscopy images were taken of (B) Oleo30G_S2C, (C) 75%/25% Oleo30G_S2C/Oleo30G, (D) 50%/50% Oleo30G_S2C/Oleo30G, (E) 25%/75% Oleo30G_S2C/Oleo30G, (F) Oleo30G. Solutions were at a total protein concentration of 80 μM in DPBS with 1 mM DTT. Samples were chilled on ice for 10 minutes before transferring to chambered coverglass coated with pluronic F-127. As the droplets are denser than water, they fell to the bottom of the chamber. Images were taken at the coverglass. Scale bar = 20 μm.

    Journal: Soft matter

    Article Title: Redox Sensitive Protein Droplets from Recombinant Oleosin

    doi: 10.1039/c8sm01047a

    Figure Lengend Snippet: (A) UV-vis spectroscopy traces of Oleo30G_S2C, Oleo30G, and Oleo30G_S2C/Oleo30G blends. Protein solutions were at a concentration of 80 μM protein in DPBS with 1 mM DTT. Blends had a total protein concentration of 80 μM. Blend molar ratios used were 75%/25%, 50%/50%, and 25%/75% Oleo30G_S2C/Oleo30G. Measurements were taken starting at 37 °C and cooling at a rate of 1 °C per minute. Measurements were taken in increments if 0.5 °C. An increase in the absorbance indicates the formation of droplets. For clarity, data is shown only for 20 °C and below. Brightfield DIC microscopy images were taken of (B) Oleo30G_S2C, (C) 75%/25% Oleo30G_S2C/Oleo30G, (D) 50%/50% Oleo30G_S2C/Oleo30G, (E) 25%/75% Oleo30G_S2C/Oleo30G, (F) Oleo30G. Solutions were at a total protein concentration of 80 μM in DPBS with 1 mM DTT. Samples were chilled on ice for 10 minutes before transferring to chambered coverglass coated with pluronic F-127. As the droplets are denser than water, they fell to the bottom of the chamber. Images were taken at the coverglass. Scale bar = 20 μm.

    Article Snippet: Protein aliquots were thawed and dialyzed into DPBS (Thermo Fisher Scientific) with 1 mM DTT.

    Techniques: UV-Vis Spectroscopy, Concentration Assay, Protein Concentration, Microscopy, Transferring

    CFHR5 competes with FH in serum for binding to PTX3, CRP, and MaxGel and causes enhanced C3 fragment deposition. ( A ) Wells were coated with 10 μg/ml PTX3, 10 μg/ml CRP, and MaxGel diluted 1:30 in DPBS. After blocking, the wells were incubated for 30 min at 37°C with 25% heat-inactivated human serum with or without 0.5 μM CFHR5 and CFHR4A. FH binding was detected with mAb A254 and the corresponding secondary Ab. The normalized values are means ± SD derived from three independent experiments. *** p

    Journal: The Journal of Immunology Author Choice

    Article Title: Factor H–Related Protein 5 Interacts with Pentraxin 3 and the Extracellular Matrix and Modulates Complement Activation

    doi: 10.4049/jimmunol.1403121

    Figure Lengend Snippet: CFHR5 competes with FH in serum for binding to PTX3, CRP, and MaxGel and causes enhanced C3 fragment deposition. ( A ) Wells were coated with 10 μg/ml PTX3, 10 μg/ml CRP, and MaxGel diluted 1:30 in DPBS. After blocking, the wells were incubated for 30 min at 37°C with 25% heat-inactivated human serum with or without 0.5 μM CFHR5 and CFHR4A. FH binding was detected with mAb A254 and the corresponding secondary Ab. The normalized values are means ± SD derived from three independent experiments. *** p

    Article Snippet: Interaction of CFHR5 with pCRP was measured in TBS containing Ca2+ and Mg2+ and with mCRP, which was generated from commercially available CRP as described , in DPBS (Lonza, Cologne, Germany).

    Techniques: Binding Assay, Blocking Assay, Incubation, Derivative Assay

    Comparison of TrypLE™ Select animal-free tyrpsin immobilization and cold PBS with cell scraping for harvesting mature HOCl-oxidized lysate-loaded DCs . (A) DCs were matured with LPS and IFN-γ for 16 h following lysate-loading. It was observed that mature lysate-loaded DCs that were harvested using TrypLE™ Select animal-free tyrpsin replacement exhibited approximately 30-40% reduction in the levels of MHC Class I ( P = 0.13; NS) and HLA-DR ( P = 0.15; NS) compared to DCs that were harvested with cold DPBS and cell scraping. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) ± SEM in research-scale Nunclon™Δ Surface T25 cm 2 flasks. P values were determined with unpaired Student's t test. NS indicated that P values were not significant. (B) Mature lysate-loaded DCs that were harvested with TrypLE™ Select animal-free tyrpsin replacement were as efficient as DCs that were harvested with cold DPBS and cell scraping in presenting MHC Class I-restricted HER-2/neu and MART-1 peptides and stimulating HER-2/neu and MART-1 CD8 + T cells in intracellular IFN-γ assessments.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Comparison of TrypLE™ Select animal-free tyrpsin immobilization and cold PBS with cell scraping for harvesting mature HOCl-oxidized lysate-loaded DCs . (A) DCs were matured with LPS and IFN-γ for 16 h following lysate-loading. It was observed that mature lysate-loaded DCs that were harvested using TrypLE™ Select animal-free tyrpsin replacement exhibited approximately 30-40% reduction in the levels of MHC Class I ( P = 0.13; NS) and HLA-DR ( P = 0.15; NS) compared to DCs that were harvested with cold DPBS and cell scraping. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) ± SEM in research-scale Nunclon™Δ Surface T25 cm 2 flasks. P values were determined with unpaired Student's t test. NS indicated that P values were not significant. (B) Mature lysate-loaded DCs that were harvested with TrypLE™ Select animal-free tyrpsin replacement were as efficient as DCs that were harvested with cold DPBS and cell scraping in presenting MHC Class I-restricted HER-2/neu and MART-1 peptides and stimulating HER-2/neu and MART-1 CD8 + T cells in intracellular IFN-γ assessments.

    Article Snippet: DC harvesting After stimulation with LPS and IFN-γ for 6 to 16 h, some of the mature lysate-loaded DCs were harvested using either TrypLE™ Select animal-free tyrpsin replacement (Gibco, Carlsbad, CA) or with cold DPBS and cell scrapers (BD Falcon™, San Jose, CA).

    Techniques:

    Urease has no effect on the survival of C . neoformans and killing of A . castellanii during co-incubation of C . neoformans and A . castellanii . A . castellanii (AC) was co-cultured with H99, ure1 Δ or ure1 Δ:: URE1 strain in DPBS with or without 7.5 mM urea. (A) The viability of A . castellanii was determined by Trypan Blue exclusion assay. The percentage of dead A . castellanii is determine by counting the number of Trypan Blue staining cells per total cell number counted. Two independent biological experiments were performed. Error bars are SD. P values shown in graph was calculated by Fisher’s exact test when each strain was compared with PBS alone at 48 h co-incubation. P > 0.05 when H99 and ure1 Δ:: URE1 were compared with ure1 Δ at 24 and 48 h by Fisher’s exact test. (B) The survival of cryptococcal strains were determined by colony form unit (CFU) after 0, 24 and 48 h. Two independent biological experiments were performed. Error bars are standard deviation (SD). P > 0.05 by Student’s t test.

    Journal: PLoS Pathogens

    Article Title: Cryptococcus neoformans urease affects the outcome of intracellular pathogenesis by modulating phagolysosomal pH

    doi: 10.1371/journal.ppat.1007144

    Figure Lengend Snippet: Urease has no effect on the survival of C . neoformans and killing of A . castellanii during co-incubation of C . neoformans and A . castellanii . A . castellanii (AC) was co-cultured with H99, ure1 Δ or ure1 Δ:: URE1 strain in DPBS with or without 7.5 mM urea. (A) The viability of A . castellanii was determined by Trypan Blue exclusion assay. The percentage of dead A . castellanii is determine by counting the number of Trypan Blue staining cells per total cell number counted. Two independent biological experiments were performed. Error bars are SD. P values shown in graph was calculated by Fisher’s exact test when each strain was compared with PBS alone at 48 h co-incubation. P > 0.05 when H99 and ure1 Δ:: URE1 were compared with ure1 Δ at 24 and 48 h by Fisher’s exact test. (B) The survival of cryptococcal strains were determined by colony form unit (CFU) after 0, 24 and 48 h. Two independent biological experiments were performed. Error bars are standard deviation (SD). P > 0.05 by Student’s t test.

    Article Snippet: Briefly, A . castellanii were washed twice with DPBS (Corning) and diluted in DPBS to appropriate density.

    Techniques: Incubation, Cell Culture, Trypan Blue Exclusion Assay, Staining, Standard Deviation

    Lhx8 knockdown accelerates mesenchyme differentiation ( A ) Schematic representation of the Lhx8 intervention and dental epithelium and dental mesenchyme recombination procedures. Dental epithelium and mesenchyme of mandibular first molar at E16.5 are separated and then the dental mesenchyme is placed onto upper chamber of Transwell cell culture insert and further incubated with either RNAi or lentivirus for 1 hr at RT. Meanwhile the dental epithelium is kept in ice cold DPBS. Then the mesenchyme and epithelium are recombined and cultured for up to 7 days. ( B and C ) Representative data showing the location ( B ) and morphology ( C ) of the tooth germ of E16.5 mandibular first molar. ( D–F ) The top view of the recombined tooth germ right after recombination ( D ), 1 day culture ( E ), 3 days culture ( F ) and 7 days culture ( G, control group; K , siLhx8 group). ( H–N ) The recombined tooth germs from control ( G–J ) and siLhx8 (K–N) were harvested at day 7 and chemically stained with HE ( H , I , L and M ) and immunostained with Dspp ( J , N ) ( n=5) . ( O–Q ) Immunofluorescence of the isolated E16.5 dental mesenchyme transfected with Lipofectamine RNAiMAX coated Cy3 labeled RNAi duplex before recombination. Strong positive Cy3 dots indicate high transfection efficiency. ( R ) Knockdown efficiency of Lhx8. ( S , T ) Quantification data of dentin area ( S ) and thickness ( T ). ( U ) Tooth development related gene expression were examined by qPCR in the recombined tooth germs 4 days after in vitro culture from both control and siLhx8 groups. Data presented are a representative of 3 experiments. de, dental epithelium; dm, dental mesenchyme; d, dentin; od, odontoblast; am, ameloblast; e, enamel. Scale bars: 1mm ( B–F, Q and K, O–Q) ; 500 μm ( I and M ); 250 μm ( J and N ); Data represent mean ± SD. * P

    Journal: Biomaterials

    Article Title: Lhx8 Mediated Wnt and TGFβ Pathways in Tooth Development and Regeneration

    doi: 10.1016/j.biomaterials.2015.06.004

    Figure Lengend Snippet: Lhx8 knockdown accelerates mesenchyme differentiation ( A ) Schematic representation of the Lhx8 intervention and dental epithelium and dental mesenchyme recombination procedures. Dental epithelium and mesenchyme of mandibular first molar at E16.5 are separated and then the dental mesenchyme is placed onto upper chamber of Transwell cell culture insert and further incubated with either RNAi or lentivirus for 1 hr at RT. Meanwhile the dental epithelium is kept in ice cold DPBS. Then the mesenchyme and epithelium are recombined and cultured for up to 7 days. ( B and C ) Representative data showing the location ( B ) and morphology ( C ) of the tooth germ of E16.5 mandibular first molar. ( D–F ) The top view of the recombined tooth germ right after recombination ( D ), 1 day culture ( E ), 3 days culture ( F ) and 7 days culture ( G, control group; K , siLhx8 group). ( H–N ) The recombined tooth germs from control ( G–J ) and siLhx8 (K–N) were harvested at day 7 and chemically stained with HE ( H , I , L and M ) and immunostained with Dspp ( J , N ) ( n=5) . ( O–Q ) Immunofluorescence of the isolated E16.5 dental mesenchyme transfected with Lipofectamine RNAiMAX coated Cy3 labeled RNAi duplex before recombination. Strong positive Cy3 dots indicate high transfection efficiency. ( R ) Knockdown efficiency of Lhx8. ( S , T ) Quantification data of dentin area ( S ) and thickness ( T ). ( U ) Tooth development related gene expression were examined by qPCR in the recombined tooth germs 4 days after in vitro culture from both control and siLhx8 groups. Data presented are a representative of 3 experiments. de, dental epithelium; dm, dental mesenchyme; d, dentin; od, odontoblast; am, ameloblast; e, enamel. Scale bars: 1mm ( B–F, Q and K, O–Q) ; 500 μm ( I and M ); 250 μm ( J and N ); Data represent mean ± SD. * P

    Article Snippet: In overexpression experiments, dental mesenchyme was incubated with Lhx8 over-expressing lentivirus, whereas dental epithelium was incubated in DPBS (HyClone, Thermo Scientific) followed by reconstitution[ ], and culture for up to 7 days[ ].

    Techniques: Cell Culture, Incubation, Staining, Immunofluorescence, Isolation, Transfection, Labeling, Expressing, Real-time Polymerase Chain Reaction, In Vitro

    Caco-2 cells were cultured in a 12 well plate until confluency. Fluorescently labeled microparticles suspended in DPBS were incubated with the Caco-2 cells for 30 minutes. After incubation, wells were washed (□) twice with HBSS to remove non-adherent

    Journal: Biomacromolecules

    Article Title: Wheat Germ Agglutinin Functionalized Complexation Hydrogels for Oral Insulin Delivery

    doi: 10.1021/bm701274p

    Figure Lengend Snippet: Caco-2 cells were cultured in a 12 well plate until confluency. Fluorescently labeled microparticles suspended in DPBS were incubated with the Caco-2 cells for 30 minutes. After incubation, wells were washed (□) twice with HBSS to remove non-adherent

    Article Snippet: P(MAA-g-EG)-PolyFluor™ 407 and P(MAA-g-EG)-PolyFluor™ 407-WGA microparticles were suspended in DPBS, pH 7.4 (Mediatech, Herndon, VA), pre-warmed to 37 °C at a concentration of 1 mg/ml.

    Techniques: Cell Culture, Labeling, Incubation

    Complement-mediated bacteriolysis activity of sera from mice inoculated with I-M28-47 and I-M28-47-114 against two different MDR A. baumannii growth conditions. The lysis activity percentages were determined using sera from mice inoculated with I-M28-47-114, I-M28-47 (control) or DPBS (placebo control) in presence of baby rabbit complement against MDR A. baumannii ( a ) cultured without imipenem treatment or ( b ) treated with 32 mg/L imipenem. The values are the means ± S.D. tested in duplicate. * P

    Journal: Scientific Reports

    Article Title: An Inactivated Antibiotic-Exposed Whole-Cell Vaccine Enhances Bactericidal Activities Against Multidrug-Resistant Acinetobacter baumannii

    doi: 10.1038/srep22332

    Figure Lengend Snippet: Complement-mediated bacteriolysis activity of sera from mice inoculated with I-M28-47 and I-M28-47-114 against two different MDR A. baumannii growth conditions. The lysis activity percentages were determined using sera from mice inoculated with I-M28-47-114, I-M28-47 (control) or DPBS (placebo control) in presence of baby rabbit complement against MDR A. baumannii ( a ) cultured without imipenem treatment or ( b ) treated with 32 mg/L imipenem. The values are the means ± S.D. tested in duplicate. * P

    Article Snippet: Antimicrobial susceptibility test A loop of A. baumannii was inoculated into DPBS (Sigma-Aldrich, Missouri, United States) until an 0.5 McFarland standard (Biomérieux, Marcy-l'Étoile, France) turbidity was obtained.

    Techniques: Activity Assay, Mouse Assay, Lysis, Cell Culture

    The anticancer effects of PGNO-media in vivo . (a) Simple scheme of animal experiment. (b) Pictures of 12 mice with melanoma syngeneic mice after 11 days of daily administration of DPBS or PGNO-media. Arrows mark the positions of tumor. (c) Pictures of tumor tissue from each mice at day 12. (d) A table showing the volume of tumor tissue from each mice and the average and standard deviation for the two groups.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Anticancer Activity of Liquid Treated with Microwave Plasma-Generated Gas through Macrophage Activation

    doi: 10.1155/2020/2946820

    Figure Lengend Snippet: The anticancer effects of PGNO-media in vivo . (a) Simple scheme of animal experiment. (b) Pictures of 12 mice with melanoma syngeneic mice after 11 days of daily administration of DPBS or PGNO-media. Arrows mark the positions of tumor. (c) Pictures of tumor tissue from each mice at day 12. (d) A table showing the volume of tumor tissue from each mice and the average and standard deviation for the two groups.

    Article Snippet: After 24 h incubation with PGNO-media, cells were stained with 10 μ M DAF-FM DA in DPBS (LB001-02, Welgene) for 20 min at 37°C.

    Techniques: In Vivo, Mouse Assay, Standard Deviation